Abstract:
:The kinetics of the hydrodynamic volume change associated with the unfolding and refolding of a globular protein can be observed using high performance size exclusion chromatography. Chromatographic profiles that evidence such dynamics can be simulated using equations in which chromatographic partitioning and the conformational transition are described in terms of a finite difference algorithm incorporating an apparent binding model to generate broad and asymmetric peaks. Application of these equations to the simple two-state unfolding transition of ribonuclease A in guanidine hydrochloride indicates that reliable kinetic parameters can be obtained using these equations.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Shalongo W,Jagannadham M,Stellwagen Edoi
10.1002/bip.360330113subject
Has Abstractpub_date
1993-01-01 00:00:00pages
135-45issue
1eissn
0006-3525issn
1097-0282journal_volume
33pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::Formation of toxic amyloid structures is believed to be associated with various late-onset neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. The fact that many proteins in addition to those that are associated with clinical conditions have the potential to form amyloid fibrils in vitro provides...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21477
更新日期:2010-10-01 00:00:00
abstract::The indirect solvent-induced effect on the free energy of binding of biopolymers is examined within the framework of classical statistical mechanics. We focus specifically on the role of the solute-solvent hydrogen bonding. In particular, we have estimated the first order solvent effect on the indirect interaction bet...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290604
更新日期:1990-05-01 00:00:00
abstract::RADA-16-I is a self-assembling peptide which forms biocompatible fibrils and hydrogels. We used molecular dynamics simulations, atomic-force microscopy, NMR spectroscopy, and thioflavin T binding assay to examine size, structure, and morphology of RADA-16-I aggregates. We used the native form of RADA-16-I (H-(ArgAlaAs...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22755
更新日期:2016-01-01 00:00:00
abstract::Natural cellulose molecules have a strong tendency of being aggregated into larger structures. Thus, the imaging of isolated individual cellulose molecules is hampered for a long time. In this work, we manage to observe, for the first time, the isolated individual natural cellulose chains on a sample surface by means ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21070
更新日期:2008-12-01 00:00:00
abstract::Poly(dimer acid-brassylic acid) [P(DA-BA)] copolymers and poly(dimer acid-pentadecandioic acid) [P(DA-PA)] copolymers were prepared by melt polycondensation of the corresponding mixed anhydride prepolymers. The copolymers were characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), dif...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20078
更新日期:2004-06-15 00:00:00
abstract::Multiple, independent sites or domains behave, on chemical change, in a manner predicted by Langmuir. Distortions of this behavior have been attributed to interactions between the domains, which vary with the progress of the changes occurring at the sites or domains. The two main models for nearest neighbor interactio...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199905)49:6<425::AID-BIP1>
更新日期:1999-05-01 00:00:00
abstract::In the native structure of hen egg white lysozyme (HEL), the amino acid sequence 87-97 (HEL 87-97) forms an amphiphilic helix, with hydrophilic residues in the sequence directed toward the solvent. A synthetic version of the HEL 87-97 sequence (with the cysteine corresponding to position 94 of HEL replaced by alanine)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360330307
更新日期:1993-03-01 00:00:00
abstract::G protein-coupled receptor kinase 2 (GRK2) plays a central role in the cellular transduction network. In particular, during chronic heart failure GRK2 is upregulated and believed to contribute to disease progression. Thereby, its inhibition offers a potential therapeutic solution to several pathological conditions. In...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22295
更新日期:2014-01-01 00:00:00
abstract::We have utilized a de novo designed two-stranded α-helical coiled-coil template to display conserved α-helical epitopes from the stem region of hemagglutinin (HA) glycoproteins of influenza A. The immunogens have all the surface-exposed residues of the native α-helix in the native HA protein of interest displayed on t...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22808
更新日期:2016-03-01 00:00:00
abstract::The comprehensive understanding of disassembly mechanism of amyloid fibrils requires nano-scale characterization of the mechanical properties of amyloid fibrils during the disassembly process. In this work, gemini surfactant C12 C6 C12 Br2 micelles were used as a probe to disassemble Aβ(1-40) fibrils. The microstructu...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22992
更新日期:2017-02-01 00:00:00
abstract::Through optimization of the linker region and key stabilizing mutations, it has been possible to improve the stability of the circularly permuted (cp) Trp-cage miniprotein. However, even the most stable Trp-cage circular permutants are still less stable than the analogous standard topology (std) Trp-cages. Extending m...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23327
更新日期:2019-12-01 00:00:00
abstract::Relevant parameters and stereochemical consequences of helices [alpha-helix, 3(10)-helix, beta-bend ribbon spiral, gamma-helix, 2.0(5)-helix, poly(Pro)(n) type-I and -II helices, and collagen triple helix] of peptides based on alpha-amino acids for use as templates in various branches of chemistry are briefly discusse...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20357
更新日期:2006-01-01 00:00:00
abstract::alpha,beta-Dehydro amino acid residues are known to constrain the peptide backbone to the beta-bend conformation. A pentapeptide containing only one alpha,beta-dehydrophenylalanine (delta Phe) residue has been synthesized and crystallized, and its solid state conformation has been determined. The pentapeptide Boc-Leu-...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360350202
更新日期:1995-02-01 00:00:00
abstract::The absorption, CD, and fluorescence emission spectra, and the fluorescence emission and depolarization lifetimes of the human granulocyte-macrophage colony-stimulating factor (hGM-CSF) and related peptides previously tested for their immunological activity, were measured in water at various pHs and temperatures to ob...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360360102
更新日期:1995-07-01 00:00:00
abstract::Dehydrophenylalanine, ΔPhe, is the most commonly studied α,β-dehydroamino acid. In nature, further modifications of the α,β-dehydroamino acids were found, for example, replacement of the C-terminal amide group by oxazole ring. The conformational properties of oxazole-dehydrophenylalanine residue (ΔPhe-Ozl), both isome...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22852
更新日期:2016-05-01 00:00:00
abstract::Ultraviolet hyperchromicity experiments indicate that in DNA duplex formation, a C-T mismatch is destabilizing in the center of a duplex, but behaves as a stable base pair at the terminus of a duplex. The C-T base pair is thought to contain two hydrogen bonds, but has thermodynamic parameters (delta Ho and delta Go of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360310908
更新日期:1991-08-01 00:00:00
abstract::Simulated ir absorption and vibrational CD (VCD) spectra of four alanine-based octapeptides, each having its main chain constrained to a different secondary structure conformation, were analyzed and compared with experimental results for several different peptides. The octapeptide simulations were based on transfer of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(20000415)53:5<380::AID-BIP
更新日期:2000-04-15 00:00:00
abstract::A novel nucleic acid model using peptide ribonucleic acid (PRNA), which contains 5'-amino-5'-deoxypyrimidine ribonucleoside as a recognition site for nucleic acids, has been designed, synthesized and applied to the external reversible control of recognition behavior of the complementary polynucleotide through the orie...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10563
更新日期:2004-01-01 00:00:00
abstract::Recent advances in the application of solid state nmr spectroscopy to uniformly aligned biopolymers have opened a window through which to view the detailed structure of biological macromolecules that are unable to be seen with standard techniques for structure determination such as x-ray diffraction. Atomic resolution...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360330513
更新日期:1993-05-01 00:00:00
abstract::Infrared spectra of the amino acid residues in H2O solution have been obtained in the 1800-1400-cm-1 region. It has been established that amino acid residues of arginine, asparagine, glutamine, aspartic and glutamic acids, lysine, tyrosine, histidine, and phenylalanine have intensive absorption in this spectral region...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360301309
更新日期:1990-01-01 00:00:00
abstract::The pressure-induced structural changes of a de novo designed four-helix bundle protein, (alpha-l-alpha)(2), in aqueous solution have been investigated by FTIR spectroscopy. Changes in the amide I' band intensity show that pressure induces disruption of tertiary interactions and stabilizes the solvated alpha-helical f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20628
更新日期:2007-02-05 00:00:00
abstract::While our understanding of the folding and structure of water-soluble proteins has progressed to the point where they can be artificially designed and produced from first principles, there has been only limited work toward the de novo design of membrane proteins. Such studies have been hindered in large part due to th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22149
更新日期:2012-01-01 00:00:00
abstract::A short peptide-based molecule has been found to form a strong hydrogel at phosphate buffer solution of pH 7.46. The hydrogel has been characterized thoroughly using various techniques including field emission scanning electron microscopy (FE-SEM), wide angle powder X-ray diffraction (PXRD), and rheological analysis. ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22915
更新日期:2017-01-01 00:00:00
abstract::In this study, we identified and chemically synthesized three cationic and amphipathic peptides (Glycinin-17, BCAS-16, and BCBS-11) from soybean proteins. These peptides had high isoelectric points, high positive net charges, and included multiple hydrophobic amino acids. Subsequently, we identified multiple functions...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23023
更新日期:2017-07-01 00:00:00
abstract::The 83-residue conopeptide (p21a) and its corresponding nonhydroxylated analog were isolated from the injected venom of Conus purpurascens. The complete conopeptide sequences were derived from Edman degradation sequencing of fragments from tryptic, chymotryptic and cyanogen bromide digestions, p21a has a unique, 10-cy...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21406
更新日期:2011-01-01 00:00:00
abstract::Although many anticancer drugs exert their biological activity by forming DNA interstrand crosslinks (ICLs), the thermodynamics of biologically relevant long crosslinked DNAs has not been intensively studied in contrast to short duplexes. Here, we carry out computer modeling of the shift of melting temperature of long...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22077
更新日期:2012-10-01 00:00:00
abstract::Because of its involvement in HIV entry, the chemokine receptor CXCR4 is an attractive target for antiretroviral drugs. Despite the large number of CXCR4 inhibitors studied, the 3D pharmacophore for binding to CXCR4 remains elusive, mainly as a result of conformational flexibility inherent in the identified ligands. I...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20508
更新日期:2006-01-01 00:00:00
abstract::This work advances bottom-up design of bioinspired materials built from peptide-amphiphiles, which are a class of bioconjugates in which a biofunctional peptide is covalently attached to a hydrophobic moiety that drives self-assembly in aqueous solution. Specifically, this work highlights the importance of peptide con...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22217
更新日期:2013-09-01 00:00:00
abstract::In this article, we review current knowledge about the silk produced by the larvae of bees, ants, and hornets [Apoidea and Vespoidea: Hymenoptera]. Different species use the silk either alone or in composites for a variety of purposes including mechanical reinforcement, thermal regulation, or humidification. The chara...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21702
更新日期:2012-06-01 00:00:00
abstract::We present a method for analysis of a fitness landscape of a biopolymer with significantly epistatic sites. The analysis is based on a quasi-additive fitness model. The fitness model is constructed with additive terms conducted by "site-fitness" and epistatic terms conducted by "pair-fitness," where the site-fitness i...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10126
更新日期:2002-07-05 00:00:00