Abstract:
:The 83-residue conopeptide (p21a) and its corresponding nonhydroxylated analog were isolated from the injected venom of Conus purpurascens. The complete conopeptide sequences were derived from Edman degradation sequencing of fragments from tryptic, chymotryptic and cyanogen bromide digestions, p21a has a unique, 10-cystine/5-disulfide 7-loop framework with extended 10-residue N-terminus and a 5-residue C-terminus tails, respectively. p21a has a 48% sequence homology with a recently described 13-cystine conopeptide, con-ikot-ikot, isolated from the dissected venom of the fish-hunting snail Conus striatus. However, unlike con-ikot-ikot, p21a does not form a dimer of dimers. MALDI-TOF mass spectrometry suggests that p21a may form a noncovalent dimer. p21a is an unusually large conotoxin and in so far is the largest isolated from injected venom. p21a provides evidence that the Conus venom arsenal includes larger molecules that are directly injected into the prey. Therefore, cone snails can utilize toxins that are comparable in size to the ones commonly found in other venomous animals.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Möller C,Marí Fdoi
10.1002/bip.21406subject
Has Abstractpub_date
2011-01-01 00:00:00pages
158-65issue
2eissn
0006-3525issn
1097-0282journal_volume
96pub_type
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