The use of spectral decomposition via the convex constraint algorithm in interpreting the CD-observed unfolding transitions of coiled coils.

abstract：:We describe here a new method for determining the conformational stability of antiparallel beta-sheets. Due to coupling between the transition dipoles, beta-sheet conformations typically exhibit a characteristic high-frequency amide I component centered at approximately 1680 cm(-1). Using one beta-sheet protein and tw...

journal_title：Biopolymers

authors： Wang T,Xu Y,Du D,Gai F

更新日期：2004-10-05 00:00:00

abstract：:Nucleolar targeting peptides (NrTPs) are a family of cell penetrating peptides (CPPs) derived from crotamine, a rattlesnake venom toxin. They were named NrTPs for their remarkable nucleolus-homing properties and have been studied for their potential as drug delivery vehicles. Live cell microscopy experiments were cond...

journal_title：Biopolymers

authors： Rodrigues M,Andreu D,Santos NC

更新日期：2015-03-01 00:00:00

abstract：:The currently understood function for Ras in signal transduction is in mediating the transmission of signals from external growth factors to the cell nucleus. Mutated forms of this GTP-binding protein are found in 30% of human cancers with particularly high prevalence in colon and pancreatic carcinomas. These mutation...

journal_title：Biopolymers

authors： Qian Y,Sebti SM,Hamilton AD

更新日期：1997-01-01 00:00:00

abstract：:The discovery of the prototype delta opioid antagonists TIPP (H-Tyr-Tic-Phe-Phe-OH) and TIP (H-Tyr-Tic-Phe-OH) in 1992 was followed by extensive structure-activity relationship studies, leading to the development of analogues that are of interest as pharmacological tools or as potential therapeutic agents. Stable TIPP...

journal_title：Biopolymers

authors： Schiller PW,Weltrowska G,Berezowska I,Nguyen TM,Wilkes BC,Lemieux C,Chung NN

更新日期：1999-01-01 00:00:00

abstract：:Vibrational spectroscopy coupled with thermogravimetry (TG) and differential scanning calorimetry (DSC) was used to characterize racemic propanoic acid, 2-methyl-5,6,7,8-tetrahydro-6-(methylamino)-1,2-naphthalenediyl ester hydrochloride (CHF-1035), which is a new DA2 dopaminergic receptor/alpha2 agonist and beta block...

journal_title：Biopolymers

authors： Taddei P,Torreggiani A,Fini G

更新日期：2002-01-01 00:00:00

abstract：:We report on an unrestrained molecular dynamics simulation of the flavin mononucleotide (FMN)-RNA aptamer. The simulated average structure maintains both cross-strand and intermolecular FMN-RNA nuclear Overhauser effects from the nmr experiments and has all qualitative features of the nmr structure including the G10-U...

journal_title：Biopolymers

authors： Schneider C,Sühnel J

更新日期：1999-09-01 00:00:00

abstract：:An examination of x-ray structures of single-cluster [4Fe-4S] proteins in the Protein Data Bank has revealed that all redox proteins and the glutamine 5-phosphoribosyl-l-pyrophosphate amidotransferase from Bacillus subtilis have a topological configuration arbitrarily designated as D, whereas the DNA repair enzyme end...

journal_title：Biopolymers

authors： Liang C,Mislow K

更新日期：1997-10-05 00:00:00

abstract：:In Monte Carlo simulations of polymeric chains, the chains are most often represented as spheres, or cylinders with flat ends. In this methodological paper, we adopt a representation of the chains as spherocylinders (continuous cylinders ending in semispheres). With such a representation the testing for chain overlap,...

journal_title：Biopolymers

authors： Yeramian E,Schaeffer F,Caudron B,Claverie P,Buc H

更新日期：1989-12-01 00:00:00

abstract：:Synthesis and CD and (13)C(alpha)-NMR studies in a near-neutral saline buffer are reported for a 65-residue peptide ((190)Tm(254)) comprising residues 190-254 of the alpha-tropomyosin chain. CD on a version disulfide cross-linked via the N-terminal cysteine side chains indicates that this dimer is highly helical and m...

journal_title：Biopolymers

authors： Emerson Holtzer M,Mints L,Hogue Angeletti R,d'Avignon DA,Holtzer A

更新日期：2001-10-05 00:00:00

abstract：:We present a method for analysis of a fitness landscape of a biopolymer with significantly epistatic sites. The analysis is based on a quasi-additive fitness model. The fitness model is constructed with additive terms conducted by "site-fitness" and epistatic terms conducted by "pair-fitness," where the site-fitness i...

journal_title：Biopolymers

authors： Aita T,Hamamatsu N,Nomiya Y,Uchiyama H,Shibanaka Y,Husimi Y

更新日期：2002-07-05 00:00:00

abstract：:Peptides based on 2-amino-2,3-dihydro-1H-cyclopenta[b]anthracene-2-carboxylic acid (antAib), a fluorescent, achiral, alpha-amino acid belonging to the class of C(i) (alpha)-->C(i) (alpha) cyclized, C(alpha,alpha)-disubstituted glycines, combined with L-Ala, up to the hexamer level, were synthesized by solution methods...

journal_title：Biopolymers

authors： Wright K,Lohier JF,Wakselman M,Mazaleyrat JP,Peggion C,Formaggio F,Toniolo C

更新日期：2007-01-01 00:00:00

abstract：:RNA and DNA molecules experience motions on a wide range of time scales, ranging from rapid localized motions to much slower collective motions of entire helical domains. The many functions of RNA in biology very often require this molecule to change its conformation in response to biological signals in the form of sm...

journal_title：Biopolymers

authors： Shajani Z,Varani G

更新日期：2007-08-05 00:00:00

abstract：:Software to automate the process of extracting molecular interactions from three-dimensional (3D) structures has been developed that records these as Biomolecular Interaction Network Database (BIND) pairwise interaction records. Full annotation of BIND records is provided through a database processing tool called MMDB...

journal_title：Biopolymers

authors： Salama JJ,Donaldson I,Hogue CW

更新日期：2001-01-01 00:00:00

abstract：:The kinetics of the hydrodynamic volume change associated with the unfolding and refolding of a globular protein can be observed using high performance size exclusion chromatography. Chromatographic profiles that evidence such dynamics can be simulated using equations in which chromatographic partitioning and the conf...

journal_title：Biopolymers

authors： Shalongo W,Jagannadham M,Stellwagen E

更新日期：1993-01-01 00:00:00

abstract：:For mimicking macromolecular crowding of DNA quadruplexes, various crowding agents have been used, typically PEG, with quadruplexes of micromolar strand concentrations. Thermal and thermodynamic stabilities of these quadruplexes increased with the concentration of the agents, the rise depended on the crowder used. A d...

journal_title：Biopolymers

authors： Kejnovská I,Vorlíčková M,Brázdová M,Sagi J

更新日期：2014-04-01 00:00:00

abstract：:The use of peptide carriers, termed "cell-penetrating peptides (CPPs)" has attracted much attention due to their potential for cellular delivery of hydrophilic molecules with pharmacological interest, overcoming the membrane barrier. These peptides are able to deliver attached cargos in a nontoxic manner, with the upt...

journal_title：Biopolymers

authors： Henriques ST,Castanho MA,Pattenden LK,Aguilar MI

更新日期：2010-01-01 00:00:00

abstract：:For the controlled synthesis of even the simplest dipeptide, the N(alpha)-amino group of one of the amino acids and the C-terminal carboxyl group of the other should both be blocked with suitable protecting groups. Formation of the desired amide bond can now occur upon activation of the free carboxyl group. After coup...

journal_title：Biopolymers

authors： Albericio F

更新日期：2000-01-01 00:00:00

abstract：:Synthetic model membrane-interactive peptides--both of natural and designed sequence--have become convenient and systematic tools for determination of how the membrane-spanning segments within integral membrane proteins confer protein structure and biology. Conformational studies on these peptides demonstrate that the...

journal_title：Biopolymers

authors： Deber CM,Li SC

更新日期：1995-01-01 00:00:00

abstract：:Gold nanoparticles are known to aggregate in the presence of proper multifunctional compounds. For those larger than 3 nm, the process can be followed with the naked eye because the surface plasmon absorption band of the particles shifts to longer wavelengths and the solution color changes from red to blue. To exploit...

journal_title：Biopolymers

authors： Martínez Á,Scrimin P

更新日期：2018-08-01 00:00:00

abstract：:A topochemical model to explain the biological activity of dendrotoxin-I (DTX-I), a potent blocker for potassium channels, was developed by searching common spatial arrangements of functionally important residues between DTX-I, alpha-dendrotoxin, dendrotoxin-K, BgK, ShK, and charybdotoxin. The first three are structur...

journal_title：Biopolymers

authors： Katoh E,Nishio H,Inui T,Nishiuchi Y,Kimura T,Sakakibara S,Yamazaki T

更新日期：2000-07-01 00:00:00

abstract：:The elucidation of structure-function relationships in insoluble elastin is often approached using elastin-like polypeptides. In this manner, the characterization of the different regions in this extensive biopolymer may be facilitated in a "piece-wise" manner. Our solid-state NMR experiments indicate that (LGGVG)n ha...

journal_title：Biopolymers

authors： Kumashiro KK,Kurano TL,Niemczura WP,Martino M,Tamburro AM

更新日期：2003-10-01 00:00:00

abstract：:Insect neuropeptides of the insect kinin class share a common C-terminal pentapeptide sequence F(1)X(1)(2)X(2)(3)W(4)G(5)-NH(2) (X(2)(3) = P, S, A) and regulate such critical physiological processes as water balance and digestive enzyme release. Analogs of the insect kinin class, in which the critical residues of F(1)...

journal_title：Biopolymers

authors： Zubrzak P,Williams H,Coast GM,Isaac RE,Reyes-Rangel G,Juaristi E,Zabrocki J,Nachman RJ

更新日期：2007-01-01 00:00:00

abstract：:The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...

journal_title：Biopolymers

authors： Subirós-Funosas R,El-Faham A,Albericio F

更新日期：2012-01-01 00:00:00

abstract：:Purified integral membrane proteins require amphipathic molecules to maintain their solubility in aqueous solutions. These complexes, in turn, are used in studies to characterise the protein structures by a variety of biophysical and structural techniques, including spectroscopy, crystallography, and cryo-electron mic...

journal_title：Biopolymers

authors： Ireland SM,Sula A,Wallace BA

更新日期：2018-08-01 00:00:00

abstract：:G protein-coupled receptor kinase 2 (GRK2) plays a central role in the cellular transduction network. In particular, during chronic heart failure GRK2 is upregulated and believed to contribute to disease progression. Thereby, its inhibition offers a potential therapeutic solution to several pathological conditions. In...

journal_title：Biopolymers

authors： Gomez-Monterrey I,Carotenuto A,Cipolletta E,Sala M,Vernieri E,Limatola A,Bertamino A,Musella S,Grieco P,Trimarco B,Novellino E,Iaccarino G,Campiglia P

更新日期：2014-01-01 00:00:00

abstract：:Cathelicidins are a numerous group of mammalian proteins that carry diverse antimicrobial peptides at the C-terminus of a highly conserved preproregion. These peptides, which become active when released from the proregion, display a remarkable variety of sizes, sequences, and structures, and in fact comprise represent...

journal_title：Biopolymers

authors： Gennaro R,Zanetti M

更新日期：2000-01-01 00:00:00

abstract：:The advent of kinetically controlled Native Chemical Ligation has permitted more efficient sequential Native Chemical Ligation reactions to take place in one pot where one or more of the peptide fragments contains an N-terminal cysteine residue and a C-terminal thioester. The reactivity of the thioester component can ...

journal_title：Biopolymers

authors： Ackrill T,Anderson DW,Macmillan D

更新日期：2010-01-01 00:00:00

abstract：:Bovine carbonmonoxy hemoglobin investigated with light scattering studies is found to dissociate from its native tetramer structure into dimers and monomers. The values of the hydrated tetramer radius, RT = 32.1 A, and the fractional dissociation vs pH, have been obtained at different ionic strengths from the autocorr...

journal_title：Biopolymers

authors： Lunelli L,Zuliani P,Baldini G

更新日期：1994-06-01 00:00:00

abstract：:The solution conformations of a hybrid sequence peptide related to the bee venom peptide apamin have been determined using two-dimensional 1H-nmr. Apamin is an 18 amino acid peptide containing a C-terminal helix that is stabilized by two disulfide bonds. The deletion of one residue (K4) of the N-terminal "scaffold" re...

journal_title：Biopolymers

authors： Volkman BF,Wemmer DE

更新日期：1997-04-05 00:00:00

abstract：:The sweet protein brazzein isolated from the fruit of the African plant, Pentadiplandra brazzeana Baillon is 2000-500 times sweeter than sucrose and consists of 54 amino acid residues with four intramolecular disulfide bonds. Brazzein was prepared by the fluoren-9-yl-methoxycarbonyl solid-phase method, and was identic...

journal_title：Biopolymers

authors： Izawa H,Ota M,Kohmura M,Ariyoshi Y

更新日期：1996-07-01 00:00:00