Flow linear dichroism and Fourier transform IR spectra reveal geometry for X-form DNA.

Abstract:

:Flow linear dichroism measurements extended into the vacuum uv region yield inclinations for the base normal from the helix axis of 21 degrees for dA and 40 degrees for dT in the X-form of poly(dAdT).poly(dAdT). These inclination angles are similar to the B form of the synthetic polymer, but the axes around which the bases incline are different. Hydrogen-bonded base pairs are consistent with the geometry for the standard B, C, D, and Z forms of natural DNA, but will not fit into the A form. Fourier transform ir spectra indicate that the X form has sugar pucker and phosphate geometry similar to B-form DNA, and supports the dinucleotide repeat with two kinds of phosphates seen in earlier work, in analogy to Z-form DNA. Clearly, X-form DNA has a unique geometry.

journal_name

Biopolymers

journal_title

Biopolymers

authors

Riazance-Lawrence JH,Kang H,Chou PJ,Johnson WC Jr,Vorlícková M

doi

10.1002/bip.360341104

subject

Has Abstract

pub_date

1994-11-01 00:00:00

pages

1469-76

issue

11

eissn

0006-3525

issn

1097-0282

journal_volume

34

pub_type

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