Cloning and characterization of novel cyclotides genes from South American plants.

abstract：:Gelsolin is an actin-severing protein whose action is initiated by Ca(2+) and inhibited by binding to phosphorylated inositol lipid or phosphoinositides. The regions of gelsolin responsible for phosphoinositide binding are comprised of residues 150-169 (G150-169) and 135-142 (G135-142). The corresponding peptides poss...

journal_title：Biopolymers

authors： Liepiņa I,Czaplewski C,Janmey P,Liwo A

更新日期：2003-01-01 00:00:00

abstract：:The spatial variation in the composition of nonexpanded biopolymer blends prepared by extrusion of mixtures of gelatin with either native or pregelatinized waxy maize starch was studied using a 30-microm aperture FTIR microspectroscopy technique. The ratio of the areas of the "saccharide" bands (953-1180 cm(-1)) and t...

journal_title：Biopolymers

authors： Mousia Z,Farhat IA,Pearson M,Chesters MA,Mitchell JR

更新日期：2001-01-01 00:00:00

abstract：:Neurokinins and their receptors are a complex system consisting of at least three endogenous agents--substance P (SP), neurokinin A (NKA), and neurokinin B (NKB)--and their corresponding receptor types, respectively, NK-1, NK-2, and NK-3. Investigations on receptors have been made using sensitive and fairly selective ...

journal_title：Biopolymers

authors： Regoli D,Dion S,Rhaleb NE,Rouissi N,Tousignant C,Jukic D,D'Orleans-Juste P,Drapeau G

更新日期：1989-01-01 00:00:00

abstract：:Our previous studies of the potential utility of the C(alpha)D(alpha) stretch frequency, nu(CD), as a tool for determining conformation in peptide systems (Mirkin and Krimm, J Phys Chem A 2004, 108, 10923-10924; 2007, 111, 5300-5303) dealt with the spectroscopic characteristics of isolated alanine peptides with alpha(...

journal_title：Biopolymers

authors： Mirkin NG,Krimm S

更新日期：2009-09-01 00:00:00

abstract：:Actinomycin-D (actD) binds to natural DNA at two different classes of binding sites, weak and strong. The affinity for these sites is highly dependent on DNA sequence and solution conditions, and the interaction appears to be purely entropic driven. Although the entropic character of this reaction has been attributed ...

journal_title：Biopolymers

authors： Ruggiero Neto J,Colombo MF

更新日期：2000-01-01 00:00:00

abstract：:Functional RNAs such as ribosomal RNA and structured domains of mRNA are targets for small molecule ligands that can act as modulators of the RNA biological activity. Natural ligands for RNA display a bewildering structural and chemical complexity that has yet to be matched by synthetic RNA binders. Comparison of natu...

journal_title：Biopolymers

authors： Hermann T

更新日期：2003-09-01 00:00:00

abstract：:Ribonuclease A is the archetype of a functionally diverse superfamily of vertebrate-specific ribonucleases. Inhibitors of its action have potential use in the elucidation of the in vivo roles of these enzymes and in the treatment of pathologies associated therewith. Derivatives of adenosine 5'-pyrophosphate are the mo...

journal_title：Biopolymers

authors： Holloway DE,Chavali GB,Leonidas DD,Baker MD,Acharya KR

更新日期：2009-12-01 00:00:00

abstract：:Piscidin 1 (Pis-1) has a high broad-spectrum activity against bacteria, fungi, and viruses but it also has a moderate hemolytic activities. To improve the antibacterial activity and to reduce toxicity, mutants Pis-1AA (G8A/G13A double mutant) and Pis-1PG (G8P mutant) have been designed based on the crystal structure o...

journal_title：Biopolymers

authors： Yuan T,Zhang X,Hu Z,Wang F,Lei M

更新日期：2012-12-01 00:00:00

abstract：:The conformation of basic fatty acid binding protein from chicken liver and the binding properties of the apo protein toward 11-dansylamino-undecanoic acid were investigated by CD and fluorescence spectroscopy. In one set of experiments the binding process was followed by the appearance of induced optical activity in ...

journal_title：Biopolymers

authors： Schievano E,Quarzago D,Spadon P,Monaco HL,Zanotti G,Peggion E

更新日期：1994-07-01 00:00:00

abstract：:A new series of oxy-peptide nucleic acids (pyrrolidine-based oxy-peptide nucleic acids = POPNAs) of four different stereoisomeric forms (cis-L, cis-D, trans-L, trans-D) have been synthesized. To find a favorable stereoisomer of POPNA for hybridization with DNA or RNA, thermodynamic parameters and conformations of the ...

journal_title：Biopolymers

authors： Kitamatsu M,Shigeyasu M,Saitoh M,Sisido M

更新日期：2006-01-01 00:00:00

abstract：:We have extended our studies of Trp/Trp to other Aryl/Aryl through-space interactions that stabilize hairpins and other small polypeptide folds. Herein we detail the NMR and CD spectroscopic features of these types of interactions. NMR data remains the best diagnostic for characterizing the common T-shape orientation....

journal_title：Biopolymers

authors： Anderson JM,Kier BL,Jurban B,Byrne A,Shu I,Eidenschink LA,Shcherbakov AA,Hudson M,Fesinmeyer RM,Andersen NH

更新日期：2016-06-01 00:00:00

abstract：:Pig citrate synthase (PCS) can be used as a model enzyme to gain some insight into the structural basis of protein thermostability. The thermal unfolding characteristics of the specific secondary structure elements within PCS were monitored in detail by following changes in its amide I band components. The result of o...

journal_title：Biopolymers

authors： Severcan F,Haris PI

更新日期：2003-08-01 00:00:00

abstract：:Two-dimensional (2-D) Raman and 2-D IR correlation spectroscopy are applied to analyze changes in the nu(4) region of the IR spectrum and in the nu(1) region of the Raman spectrum during the maturation of hydroxyapatite (HA) following the solution-mediated conversion of amorphous calcium phosphate (ACP) to HA. The nu(...

journal_title：Biopolymers

authors： Ou-Yang H,Paschalis EP,Boskey AL,Mendelsohn R

更新日期：2000-01-01 00:00:00

abstract：:Software to automate the process of extracting molecular interactions from three-dimensional (3D) structures has been developed that records these as Biomolecular Interaction Network Database (BIND) pairwise interaction records. Full annotation of BIND records is provided through a database processing tool called MMDB...

journal_title：Biopolymers

authors： Salama JJ,Donaldson I,Hogue CW

更新日期：2001-01-01 00:00:00

abstract：:The interhelical interfaces have been examined in seven high-resolution globin chains. The profiles of hydrophobic contact, as measured by the residue solvent-accessible area loss upon folding, have been calculated. The seven globins studied differ in their overall loss of solvent-accessible area upon packing of their...

journal_title：Biopolymers

authors： Weaver DL

更新日期：1992-05-01 00:00:00

abstract：:Vibrational absorption and vibrational circular dichroism (VCD) spectra of valinomycin are measured, in different solvents, in the ester and amide carbonyl stretching regions. The influence of cations, namely Li(+), Na(+), K(+), and Cs(+), in methanol-d(4) solvent is also investigated. Ab initio quantum mechanical cal...

journal_title：Biopolymers

authors： Wang F,Zhao C,Polavarapu PL

更新日期：2004-09-01 00:00:00

abstract：:We report on an unrestrained molecular dynamics simulation of the flavin mononucleotide (FMN)-RNA aptamer. The simulated average structure maintains both cross-strand and intermolecular FMN-RNA nuclear Overhauser effects from the nmr experiments and has all qualitative features of the nmr structure including the G10-U...

journal_title：Biopolymers

authors： Schneider C,Sühnel J

更新日期：1999-09-01 00:00:00

abstract：:The methods used to study the relative reaction rates of 45 different aliphatic and aromatic carboxylic acids when coupled to resin-bound amino acid amides is described. Competition experiments involving the coupling of incoming carboxylic acids to resin-bound amino acid amides were performed. The relative composition...

journal_title：Biopolymers

authors： Acharya AN,Ostresh JM,Houghten RA

更新日期：2002-10-05 00:00:00

abstract：:The process of protein folding is obviously driven by forces exerted on the atoms of the amino-acid chain. These forces arise from interactions with other parts of the protein itself (direct forces), as well as from interactions with the solvent (solvent-induced forces). We present a statistical-mechanical formalism t...

journal_title：Biopolymers

authors： Durell SR,Ben-Naim A

更新日期：2017-08-01 00:00:00

abstract：:The de novo design of peptides and proteins has emerged as an approach for investigating protein structure and function. The success relies heavily on the ability to design relatively short peptides that can adopt stable secondary structures. To this end, substitution with α,β-dehydroamino acids, especially α,β-didehy...

journal_title：Biopolymers

authors： Gupta M,Chauhan VS

更新日期：2011-03-01 00:00:00

abstract：:In recent years, a revolution has occurred in the basic sciences, which exploits novel single molecule detection and manipulation tools to track and analyze biopolymers in unprecedented detail. A recent Gordon Research Conference style meeting, hosted by the University of Michigan, highlighted current status and futur...

journal_title：Biopolymers

authors： Walter NG,Meiners JC,Meyhofer E,Neubig RR,Sunahara RK,Perkins NC,Steel DG,Swanson JA

更新日期：2007-02-05 00:00:00

abstract：:Multiconfiguration thermodynamic integration was used to determine the relative binding strength of tacrine and 6-chlorotacrine by Torpedo californica acetylcholinesterase. 6-Chlorotacrine appears to be bound stronger by 0.7+/-0.4 kcal/mol than unsubstituted tacrine when the active site triad residue His-440 is deprot...

journal_title：Biopolymers

authors： Wlodek ST,Antosiewicz J,McCammon JA,Straatsma TP,Gilson MK,Briggs JM,Humblet C,Sussman JL

更新日期：1996-01-01 00:00:00

abstract：:The creation of peptide libraries by site-selective modification of a few peptide substrates would increase the efficiency of discovery processes, but still is a real synthetic challenge. The site-selective modification of small peptides at serine or threonine residues, by using a short scission-addition procedure, al...

journal_title：Biopolymers

authors： Romero-Estudillo I,Saavedra C,Boto A,Álvarez E

更新日期：2015-09-01 00:00:00

abstract：:The solid state conformational analysis of [Tyr4] cyclolinopeptide A has been carried out by x-ray diffraction studies. The crystal structure of the monoclinic form, grown from a dioxane-water mixture [alpha = 9.849 (5) A, b = 20.752 (4) A, c = 16.728 (5) A, beta = 98.83 (3) degrees, space group P21, Z = 2], shows the...

journal_title：Biopolymers

authors： Saviano M,Rossi F,Filizola M,Isernia C,Di Blasio B,Benedetti E,Pedone C,Siemion IZ,Pedyczak A

更新日期：1995-10-01 00:00:00

abstract：:DNA sequences containing four types of analog nucleosides are described. All four are pyridine derivatives constructed as C-nucleosides so that they mimic the pyrimidine derivatives 2'-deoxyuridine, thymidine or 2'-deoxycytidine, but in all cases the analogs lack the corresponding O2-carbonyls that in duplex DNA are l...

journal_title：Biopolymers

authors： Sun Z,McLaughlin LW

更新日期：2007-10-05 00:00:00

abstract：:Solid state nmr methods were applied to the study of the motions and structural heterogeneity in polyglycine, sodium poly(L-glutamate), and poly(L-alanine). The response of both the main-chain and side-chain resonances to the addition of water was studied using static and magic-angle sample-spinning line shapes as wel...

journal_title：Biopolymers

authors： Kennedy SD,Bryant RG

更新日期：1990-01-01 00:00:00

abstract：:Recently, the use of hybrid double network (DN) hydrogels has become prominent due to their enhanced mechanical properties, which has opened the door for new applications of these soft materials. Only a few of these gels have demonstrated both injectable and moldable capabilities. In this work, we report the mechanica...

journal_title：Biopolymers

authors： Qiao Z,Mieles M,Ji HF

更新日期：2020-06-01 00:00:00

abstract：:The kinetics of the hydrodynamic volume change associated with the unfolding and refolding of a globular protein can be observed using high performance size exclusion chromatography. Chromatographic profiles that evidence such dynamics can be simulated using equations in which chromatographic partitioning and the conf...

journal_title：Biopolymers

authors： Shalongo W,Jagannadham M,Stellwagen E

更新日期：1993-01-01 00:00:00

abstract：:A series of CD measurements were carried out on members of peptides in the endothelin and sarafotoxin families. The helical structures taken by these peptides containing the helical motif with the sequences of Cys-X-X-X-Cys and Cys-X-Cys [Y. Kobayashi et al. (1991) Neurochemistry International Vol. 18, pp. 525-534] ar...

journal_title：Biopolymers

authors： Tamaoki H,Kyogoku Y,Nakajima K,Sakakibara S,Hayashi M,Kobayashi Y

更新日期：1992-04-01 00:00:00

abstract：:Gold nanoparticles are known to aggregate in the presence of proper multifunctional compounds. For those larger than 3 nm, the process can be followed with the naked eye because the surface plasmon absorption band of the particles shifts to longer wavelengths and the solution color changes from red to blue. To exploit...

journal_title：Biopolymers

authors： Martínez Á,Scrimin P

更新日期：2018-08-01 00:00:00