Abstract:
:We review the neighbor exclusion model and some of its applications to analyze the binding data of DNA-ligand complexes. We revisit the closed form of the model developed by McGhee and von Hippel in 1974, showing that this classic model can be used to help studying the behavior of DNA contour and persistence lengths when interacting with intercalating ligands. We present methods to quantitatively analyze the variation of these two quantities, allowing one to determine important parameters of the interaction such as the intrinsic binding constant and the exclusion number of the ligand.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Rocha MSdoi
10.1002/bip.21296subject
Has Abstractpub_date
2010-01-01 00:00:00pages
1-7issue
1eissn
0006-3525issn
1097-0282journal_volume
93pub_type
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