Abstract:
:The design of biomimetic materials through molecular self-assembly is a growing area of modern nanotechnology. With problems of protein folding, self-assembly, and sequence-structure relationships as essential in nanotechnology as in biology, the effect of the nucleation of β-hairpin formation by proline on the folding process has been investigated in model studies. Previously such studies were limited to investigations of the influence of proline on the formation of turns in short peptide sequences. The effect of proline-based triads on the folding of an 11-kDa amyloidogenic peptide GH6[(GA)3GY(GA)3GE]8 GAH6 (YE8) was investigated by selective substitution of the proline-substituted triads at the γ-turn sites. The folding and fibrillation of the singly proline-substituted polypeptides, e.g., GH6-[(GA)3GY(GA)3GE]7(GA)3GY(GA)3PD-GAH6 (8PD), and doubly proline-substituted polypeptides, e.g., GH6-[(GA)3GY(GA)3GE]3(GA)3GY(GA)3PD[(GA)3GY(GA)3GE]3(GA)3GY(GA)3PD-GAH6 (4,8PD), were directly monitored by circular dichroism and deep UV resonance Raman and fluorescence spectroscopies. These findings were used to identify the essential folding domains, i.e., the minimum number of β-strands necessary for stable folding. These experimental findings may be especially useful in the design and construction of peptidic materials for a wide range of applications as well as in understanding the mechanisms of folding critical to fibril formation.
journal_name
Biopolymersjournal_title
Biopolymersauthors
Takor GA,Higashiya S,Sikirzhytski VK,Seeley JP,Lednev IK,Welch JTdoi
10.1002/bip.22622subject
Has Abstractpub_date
2015-06-01 00:00:00pages
339-50issue
6eissn
0006-3525issn
1097-0282journal_volume
103pub_type
杂志文章相关文献
BIOPOLYMERS文献大全abstract::The equilibrium structural ensemble of a 20-residue polyglutamic acid peptide (E(20)) was studied with FRET, circular dichroism, and molecular dynamics (MD) simulations. A FRET donor, o-aminobenzamide, and acceptor, 3-nitrotyrosine, were introduced at the N- and C-termini, respectively. Circular dichroism, steady stat...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20719
更新日期:2007-06-15 00:00:00
abstract::Bombolitins are five structurally related heptadecapeptides originally isolated from the venom of a bumblebee, acting at membrane level and able to enhance the activity of Phospholipase A2. The biological activity of this class of natural peptides seems to be related to the their ability to form amphiphilic helical st...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(1997)43:6<419::AID-BIP4>3.
更新日期:1997-01-01 00:00:00
abstract::Molecular structures for parallel DNA and RNA double helices with Hoogsteen pairing are proposed for the first time. The DNA helices have sugars in the C2'-endo region and the phosphodiester conformations are (trans, gauche-), and the RNA helices have sugars in the C3'-endo region and the phosphodiester conformations ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360341202
更新日期:1994-12-01 00:00:00
abstract::Additive physical properties of DNA double strand polymers have been expanded in terms of 8 irreducible parameters. This provided consistency relations among the corresponding 10 duplex dimer contributions. To allow for oligomer analysis, end parameters were often added, and this would add extra degrees of freedom to ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22214
更新日期:2013-08-01 00:00:00
abstract::Fe(III)-Mimochrome VI (MC6) is a recently reported artificial heme-peptide conjugate system with a high peroxidase-like activity. By design, its structure features a five-coordinated Fe(III)-deuteroporphyrin active site, embedded in a compact α-helix-heme-α-helix "sandwich" motif. Up to now, no detailed MC6 structural...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23225
更新日期:2018-08-01 00:00:00
abstract::The development of differential scanning calorimetry has resulted in an increased interest in studies of the unfolding process in proteins with the aim of identifying domains and interactions with ligands or other proteins. Several of these studies were done with actin and showed that the thermal unfolding of F-actin ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20567
更新日期:2006-11-01 00:00:00
abstract::Hepcidin is a four disulfide 25-residue peptide hormone which has a central role in the regulation of iron homeostasis. To support studies on hepcidin we have sought to establish reliable and robust synthetic methods for the preparation of correctly folded materials. While correctly-folded hepcidin has good aqueous so...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21383
更新日期:2010-01-01 00:00:00
abstract::Partially acetylated, high molecular weight glucuronans were produced by a Sinorhizobium meliloti mutant strain. Two native glucuronan samples with various degrees of acetylation were sonicated to obtain lower molecular weight samples and with low viscosity suitable for chemical modification and (13)C NMR experiments....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10134
更新日期:2002-06-01 00:00:00
abstract::The aim of this study was to develop a nondestructive method to quantitate relative amounts of n-3 and n-6 polyunsaturated fatty acid (PUFA) species in vegetable oils and oil seeds using Fourier transform IR spectroscopy (FTIR). The alkene Cbond;H stretching vibrations of unsaturated fatty acids in oils showed IR abso...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10505
更新日期:2003-12-01 00:00:00
abstract::The role of phosphorylation in stabilizing the N-termini of alpha-helices is examined using computer simulations of model peptides. The models comprise either a phosphorylated or unphosphorylated serine at the helix N-terminus, followed by nine alanines. Monte Carlo/stochastic Dynamics simulations were performed on th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(199903)49:3<225::AID-BIP4>
更新日期:1999-03-01 00:00:00
abstract::Despite the positive results observed in vitro and in vivo, clinical trials with bioactive peptides are generally hampered by their fast degradation in the biological system. Two bioactive peptides, P20 (CSSRTMHHC) and the combined peptide C (CVNHPAFACGYGHTMYYHHYQHHL) have been identified as anticancer therapeutics. C...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.23029
更新日期:2017-09-01 00:00:00
abstract::Time-resolved Förster resonance energy transfer (trFRET) has been used to obtain interdye distance distributions. These distributions give the most probable distance as well as a parameter, sigma, that characterize the width of the distribution. This latter parameter contains information not only on the flexibility of...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10138
更新日期:2001-01-01 00:00:00
abstract::Inhibitor design against viral targets must take into account the peculiar characteristics of viral biology-in particular, the plasticity of their replicative machinery. This includes maturational cleavage of the polyprotein, which is mediated by virally encoded proteases. Designing against a movable target is particu...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.10230
更新日期:2002-01-01 00:00:00
abstract::Cathelicidins are a numerous group of mammalian proteins that carry diverse antimicrobial peptides at the C-terminus of a highly conserved preproregion. These peptides, which become active when released from the proregion, display a remarkable variety of sizes, sequences, and structures, and in fact comprise represent...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/1097-0282(2000)55:1<31::AID-BIP40>3.0.CO;2
更新日期:2000-01-01 00:00:00
abstract::The present study characterizes the unfolding and folding processes of recombinant manganese peroxidase. This enzyme contains five disulfide bonds, two calcium ions, and one heme prosthetic group. Circular dichroism in the far UV was used to monitor global changes of the protein secondary structure, whereas UV-visible...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10283
更新日期:2003-01-01 00:00:00
abstract::Synthetic RNA poly[r(A-T)] has been synthesized and its CD spectral properties compared to those of poly[r(A-U)], poly[d(A-T)], and poly[d(A-U)] in various salt and ethanolic solutions. The CD spectra of poly[r(A-T)] in an aqueous buffer and of poly[d(A-T)] in 70.8% v/v ethanol are very similar, suggesting that they b...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.360290210
更新日期:1990-02-05 00:00:00
abstract::Structural formation of segments plays pivotal roles in protein folding and stability, but how the segment influences the structural ensemble remains elusive. We engineered two hybrid proteins by replacing the central helical segment of immunoglobulin G binding domain of streptococcal protein G with an alpha-helix or ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20283
更新日期:2005-09-01 00:00:00
abstract::Circular dichroism using synchrotron radiation (SRCD) can extend the spectral range down to approximately 130 nm for dry proteins, potentially providing new structural information. Using a selection of dried model proteins, including alpha-helical, beta-sheet, and mixed-structure proteins, we observe a low-wavelength ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21011
更新日期:2008-09-01 00:00:00
abstract::A new family of small plant peptides was recently described and found to be widespread throughout the Millereae and Heliantheae tribes of the sunflower family Asteraceae. These peptides originate from the post-translational processing of unusual seed-storage albumin genes, and have been termed PawS-derived peptides (P...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22901
更新日期:2016-11-01 00:00:00
abstract::Simulated ir absorption and vibrational CD (VCD) spectra of four alanine-based octapeptides, each having its main chain constrained to a different secondary structure conformation, were analyzed and compared with experimental results for several different peptides. The octapeptide simulations were based on transfer of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0282(20000415)53:5<380::AID-BIP
更新日期:2000-04-15 00:00:00
abstract::MD simulations have been carried out to understand the dynamical behavior of the DNA substrate of the Thermus aquaticus DNA methyltransferase (M.TaqI) in the methylation process at N6 of adenine. As starting structures, an x-ray structure of M.TaqI in complex with DNA and cofactor analogue (PDB code: 1G 38) and free d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20341
更新日期:2005-10-15 00:00:00
abstract::Insect neuropeptides of the insect kinin class share a common C-terminal pentapeptide sequence F(1)X(1)(2)X(2)(3)W(4)G(5)-NH(2) (X(2)(3) = P, S, A) and regulate such critical physiological processes as water balance and digestive enzyme release. Analogs of the insect kinin class, in which the critical residues of F(1)...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20638
更新日期:2007-01-01 00:00:00
abstract::A water-insoluble polysaccharide (TM8) was isolated from sclerotium of Pleurotus tuber-regium by extraction with 0.5M NaOH aqueous solutions at 120 degrees C. Its chemical structure was confirmed by infrared, high performance liquid chromatography, gas chromatography, and (13)C NMR in dimethylsulfoxide (DMSO) to be co...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200111)59:6<457::AID-BIP1050>3.0
更新日期:2001-11-01 00:00:00
abstract::Cytochrome c3 from Desulfovibrio gigas is electrostatically adsorbed on Ag electrodes coated with self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid. The redox equilibria and electron transfer dynamics of the adsorbed four-heme protein are studied by surface enhanced resonance Raman spectroscopy. Immobiliz...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10101
更新日期:2002-01-01 00:00:00
abstract::Electronic absorbance and fluorescence titrations are used to probe the interaction of chlorobenzylidine with DNA. The binding of chlorobenzylidine to DNA results in hypochromism, a small shift to a longer wavelength in the absorption spectra, and emission quenching in the fluorescence spectra. These spectral characte...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10001
更新日期:2001-01-01 00:00:00
abstract::The use of peptide carriers, termed "cell-penetrating peptides (CPPs)" has attracted much attention due to their potential for cellular delivery of hydrophilic molecules with pharmacological interest, overcoming the membrane barrier. These peptides are able to deliver attached cargos in a nontoxic manner, with the upt...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21367
更新日期:2010-01-01 00:00:00
abstract::This work concerns the aggregation properties of (Gly-Val-Gly-Val-Pro)(251) rec, a polypentapeptide reflecting a highly conserved repetitive unit of the bioelastomer, elastin. On raising the temperature of aqueous solutions above 25 degrees C, this polypeptide was already known to undergo concurrent conformational cha...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/1097-0282(200107)59:1<51::AID-BIP1005>3.0.
更新日期:2001-07-01 00:00:00
abstract::The various motifs of RNA molecules are closely related to their structural and functional properties. To better understand the nature and distributions of such structural motifs (i.e., paired and unpaired bases in stems, junctions, hairpin loops, bulges, and internal loops) and uncover characteristic features, we ana...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10525
更新日期:2004-02-15 00:00:00
abstract::Inherent chemical programmability available in peptide-based hydrogels has allowed diversity in the development of these materials for use in biomedical applications. Within the 20 natural amino acids, a range of chemical moieties are present. Here we used a mixing-induced self-assembly of two oppositely charged pepti...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.22194
更新日期:2013-04-01 00:00:00
abstract::The enhanced nucleophilicity and redox sensitivity of the thiol group renders cysteine residues susceptible to numerous electrophilic and oxidative post-translational modifications to form disulfides, sulfenic acids, nitrosothiols, and lipid-modified species. Outside of these well-characterized modifications of cystei...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.22252
更新日期:2014-02-01 00:00:00