Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. II. Amide absorption bands of polypeptides and fibrous proteins in alpha-, beta-, and random coil conformations.

abstract：:Decomposition of CD spectra for the unfolding of both coiled-coil and single-helical molecules is carried out via the convex constraint algorithm (CCA) [A. Perczel, M. Hollósi, G. Tusnády, and G. D. Fasman (1991) Protein Engineering, Vol. 4, pp. 669-679]. Examined are (1) our thermal unfolding data for rabbit alpha al...

journal_title：Biopolymers

authors： Holtzer ME,Holtzer A

更新日期：1995-09-01 00:00:00

abstract：:While our understanding of the folding and structure of water-soluble proteins has progressed to the point where they can be artificially designed and produced from first principles, there has been only limited work toward the de novo design of membrane proteins. Such studies have been hindered in large part due to th...

journal_title：Biopolymers

authors： Tulumello DV,Johnson RM,Isupov I,Deber CM

更新日期：2012-01-01 00:00:00

abstract：:The experimental thermodynamic data for the dissolution of five simple hydrocarbon molecules in water were combined with the solute-solvent interaction energy from a computer simulation study to yield data on the enthalpy change of solvent reorganization. Similar data were generated for dissolving these same solute mo...

journal_title：Biopolymers

authors： Lee B

更新日期：1991-07-01 00:00:00

abstract：:The title compound represents the smallest member of cyclic proline peptides corresponding to the general formula c(DDLL-Pro4)n with a strictly D,D,L,L double-alternating sequence of the chiral amino acid residues. The cyclopeptides with n greater than or equal to 2 could be synthesized from both DDLL-Pro4 (1) and DLL...

journal_title：Biopolymers

authors： Mästle W,Link U,Witschel W,Thewalt U,Weber T,Rothe M

更新日期：1991-05-01 00:00:00

abstract：:Molecular modeling is used to demonstrate that the major structural deformations of DNA caused by four different minor groove binding proteins, TBP, SRY, LEF-1, and PurR, can all be mimicked by stretching the double helix between two 3'-phosphate groups flanking the binding region. This deformation reproduces the wide...

journal_title：Biopolymers

authors： Lebrun A,Lavery R

更新日期：1999-04-15 00:00:00

abstract：:Alpha-synuclein (alpha-Syn) has been identified as a component of intracellular fibrillar deposits in Parkinson's disease. Though the real pathogenesis is still unknown, many investigations have revealed that conformational alteration and fibril formation of alpha-Syn protein have an important role in causing the dise...

journal_title：Biopolymers

authors： Lin XJ,Zhang F,Xie YY,Bao WJ,He JH,Hu HY

更新日期：2006-10-15 00:00:00

abstract：:A suite of FORTRAN programs, PREF, is described for calculating preference functions from the data base of known protein structures and for comparing smoothed profiles of sequence-dependent preferences in proteins of unknown structure. Amino acid preferences for a secondary structure are considered as functions of a s...

journal_title：Biopolymers

authors： Juretić D,Lee B,Trinajstić N,Williams RW

更新日期：1993-02-01 00:00:00

abstract：:The presence of non-native kinetic traps in the free energy landscape of a protein may significantly lengthen the overall folding time so that the folding process becomes unreliable. We use a computational model alpha-helical hairpin peptide to calculate structural free energy landscapes and relate them to the kinetic...

journal_title：Biopolymers

authors： Chapagain PP,Gerstman BS

更新日期：2006-02-15 00:00:00

abstract：:The solution structure of a hexapeptide, cyclo (Gln-Trp-Phe-Gly-Leu-Met), which is a selective NK-2 antagonist, has been studied by a combination of two-dimensional nmr and molecular dynamics (MD) techniques. The simulation based on nmr and MD data resulted in the convergence to a family of structures. Free molecular ...

journal_title：Biopolymers

authors： Zhang M,Quinn TP,Wong TC

更新日期：1994-09-01 00:00:00

abstract：:Dynamic and static intensity light scattering techniques were employed to identify conditions allowing preparation of homogeneous solutions of distinct oligomeric states of RecA protein. These hydrodynamically distinguishable oligomer populations of RecA protein were obtained in homogeneous pure quantities sufficient ...

journal_title：Biopolymers

authors： Budzynski DM,Gao X,Benight AS

更新日期：1996-04-01 00:00:00

abstract：:Cyclotides are multifunctional plant cyclic peptides containing 28-37 amino acid residues and a pattern of three disulfide bridges, forming a motif known as the cyclic cystine knot. Due to their high biotechnological potential, the sequencing and characterization of cyclotide genes are crucial not only for cloning and...

journal_title：Biopolymers

authors： Cunha NB,Barbosa AE,de Almeida RG,Porto WF,Maximiano MR,Álvares LC,Munhoz CB,Eugênio CU,Viana AA,Franco OL,Dias SC

更新日期：2016-11-01 00:00:00

abstract：:Flow linear dichroism measurements extended into the vacuum uv region yield inclinations for the base normal from the helix axis of 21 degrees for dA and 40 degrees for dT in the X-form of poly(dAdT).poly(dAdT). These inclination angles are similar to the B form of the synthetic polymer, but the axes around which the ...

journal_title：Biopolymers

authors： Riazance-Lawrence JH,Kang H,Chou PJ,Johnson WC Jr,Vorlícková M

更新日期：1994-11-01 00:00:00

abstract：:In the native structure of hen egg white lysozyme (HEL), the amino acid sequence 87-97 (HEL 87-97) forms an amphiphilic helix, with hydrophilic residues in the sequence directed toward the solvent. A synthetic version of the HEL 87-97 sequence (with the cysteine corresponding to position 94 of HEL replaced by alanine)...

journal_title：Biopolymers

authors： Vuilleumier S,Mutter M

更新日期：1993-03-01 00:00:00

abstract：:Myosins are a large family of molecular motors that use the common P-loop, Switch 1 and Switch 2 nucleotide binding motifs to recognize ATP, to create a catalytic site than can efficiently hydrolyze ATP and to communicate the state of the nucleotide pocket to other allosteric binding sites on myosin. The energy of ATP...

journal_title：Biopolymers

authors： Geeves MA

更新日期：2016-08-01 00:00:00

abstract：:A highly potent and selective agonist to the tachykinin NK-3 receptor, [pGlu6,N-MePhe8,Aib9] substance P (6-11) (I), was synthesized via the solid phase method. The ED50 of I was 4 nM in the guinea pig ileum in the absence of atropine (NK-1+NK-3 receptors) and this agonist was 5000-fold less potent in the presence of ...

journal_title：Biopolymers

authors： Tallon M,Ron D,Halle D,Amodeo P,Saviano G,Temussi PA,Selinger Z,Naider F,Chorev M

更新日期：1993-06-01 00:00:00

abstract：:Although the structural analysis of membrane proteins is advancing, an understanding of the basic principles that underlie their folding and assembly remains limited because of the high insolubility intrinsic to these molecules and concomitant challenges in obtaining crystals. Fortunately, from an experimental standpo...

journal_title：Biopolymers

authors： Rath A,Johnson RM,Deber CM

更新日期：2007-01-01 00:00:00

abstract：:The oxidation of methionine to methionine sulfoxide both in vivo and in vitro can lead to the loss of biological activity in a variety of proteins. This loss of activity can be reversed by an enzyme called methionine sulfoxide reductase. The gene for this enzyme has been cloned and sequenced from a variety of prokaryo...

journal_title：Biopolymers

authors： Brot N,Weissbach H

更新日期：2000-01-01 00:00:00

abstract：:Conformational analysis of the neurohypophyseal hormones oxytocin (OT) and arginine-vasopressin (AVP) has been carried out using two different computational approaches and three force fields, namely by the Electrostatically Driven Monte Carlo (EDMC) method, with the Empirical Conformational Energy Program for Peptides...

journal_title：Biopolymers

authors： Liwo A,Tempczyk A,Ołdziej S,Shenderovich MD,Hruby VJ,Talluri S,Ciarkowski J,Kasprzykowski F,Lankiewicz L,Grzonka Z

更新日期：1996-02-01 00:00:00

abstract：:Aqueous solutions of DNA fragments with a contour length (500 A) near the persistence length at DNA concentrations ranging from 10 to 290 mg/mL solvent and a constant supporting electrolyte concentration of 0.01 M (predominantly NaCl) were examined by 23Na-nmr spectroscopy at temperatures of 20, 40, and 60 degrees C. ...

journal_title：Biopolymers

authors： Strzelecka TE,Rill RL

更新日期：1990-01-01 00:00:00

abstract：:Simulated ir absorption and vibrational CD (VCD) spectra of four alanine-based octapeptides, each having its main chain constrained to a different secondary structure conformation, were analyzed and compared with experimental results for several different peptides. The octapeptide simulations were based on transfer of...

journal_title：Biopolymers

authors： Bour P,Kubelka J,Keiderling TA

更新日期：2000-04-15 00:00:00

abstract：:In this study, we identified and chemically synthesized three cationic and amphipathic peptides (Glycinin-17, BCAS-16, and BCBS-11) from soybean proteins. These peptides had high isoelectric points, high positive net charges, and included multiple hydrophobic amino acids. Subsequently, we identified multiple functions...

journal_title：Biopolymers

authors： Taniguchi M,Saito K,Nomoto T,Namae T,Ochiai A,Saitoh E,Tanaka T

更新日期：2017-07-01 00:00:00

abstract：:RADA-16-I is a self-assembling peptide which forms biocompatible fibrils and hydrogels. We used molecular dynamics simulations, atomic-force microscopy, NMR spectroscopy, and thioflavin T binding assay to examine size, structure, and morphology of RADA-16-I aggregates. We used the native form of RADA-16-I (H-(ArgAlaAs...

journal_title：Biopolymers

authors： Bagrov D,Gazizova Y,Podgorsky V,Udovichenko I,Danilkovich A,Prusakov K,Klinov D

更新日期：2016-01-01 00:00:00

abstract：:Peptide-conjugated polysaccharide matrices using bioactive laminin-derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide-polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde-alginate was s...

journal_title：Biopolymers

authors： Fujimori C,Kumai J,Nakamura K,Gu Y,Katagiri F,Hozumi K,Kikkawa Y,Nomizu M

更新日期：2017-01-01 00:00:00

abstract：:Despite the positive results observed in vitro and in vivo, clinical trials with bioactive peptides are generally hampered by their fast degradation in the biological system. Two bioactive peptides, P20 (CSSRTMHHC) and the combined peptide C (CVNHPAFACGYGHTMYYHHYQHHL) have been identified as anticancer therapeutics. C...

journal_title：Biopolymers

authors： Arruda DC,de Oliveira TD,Cursino PHF,Maia VSC,Berzaghi R,Travassos LR,Tada DB

更新日期：2017-09-01 00:00:00

abstract：:The presence of low pKa N-hydroxylamines is beneficial in peptide chemistry as they reduce some base-mediated side reactions. Here we evaluated the applicability and buffering capacity of Ethyl 2-cyano-2-(hydroxyimino) acetate (Oxyma) in the prevention of aspartimide/piperidide formation and Pro-based overcoupling and...

journal_title：Biopolymers

authors： Subirós-Funosas R,El-Faham A,Albericio F

更新日期：2012-01-01 00:00:00

abstract：:In our continuing series of measurements of the complex index of refraction for representative samples of biological materials, we measured ovalbumin (egg albumin) over the spectral region from 0.130 (76,923 cm(-1)) to 2.50 microm (4000 cm(-1)). Films of ovalbumin suitable for optical analyses were prepared and measur...

journal_title：Biopolymers

authors： Arakawa ET,Tuminello PS,Khare BN,Milham ME

更新日期：2001-01-01 00:00:00

abstract：:The interaction of an RGD-containing epitope from the hepatitis A virus VP3 capsid protein and its RGA-analogue with lipid membranes was studied by biophysical methods. Two types of model membrane were used: vesicles and monolayers spread at the air/water interface, with a composition that closely resembles the lipid ...

journal_title：Biopolymers

authors： Chávez A,Pujol M,Haro I,Alsina MA,Cajal Y

更新日期：2001-01-01 00:00:00

abstract：:This work advances bottom-up design of bioinspired materials built from peptide-amphiphiles, which are a class of bioconjugates in which a biofunctional peptide is covalently attached to a hydrophobic moiety that drives self-assembly in aqueous solution. Specifically, this work highlights the importance of peptide con...

journal_title：Biopolymers

authors： Marullo R,Kastantin M,Drews LB,Tirrell M

更新日期：2013-09-01 00:00:00

abstract：:From Auricularia auricula-judae, a water soluble beta-D-glucan, named as AAG, was isolated by extraction with 70% ethanol/water solution. Its chemical structure was analyzed by gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), matrix-assisted laser desorption /ionization (MALDI)-time of flight (TO...

journal_title：Biopolymers

authors： Ma Z,Wang J,Zhang L

更新日期：2008-07-01 00:00:00

abstract：:A three-dimensional model of DNA/RNA triple helix that contains a poly(L-deoxyadenosine) (L-dA) chain is proposed based on computer-assisted model building and energy calculations. The model building was performed by a new method that systematically searches possible conformations of nucleotide units in the helical ch...

journal_title：Biopolymers

authors： Tomioka N,Itai A

更新日期：1992-12-01 00:00:00