Biopolymer excreted by pseudoalteromonas antarctica NF(3), as a coating and protective agent of liposomes against dodecyl maltoside

Abstract:

:The ability of an exopolymer of glycoproteic character (GP) excreted by a new gram-negative species Pseudoalteromonas antarctica NF(3), to coat phosphatidylcholine (PC) liposomes and to protect these bilayers against the action of the nonionic surfactant dodecyl maltoside was investigated. Transmission electron microscopy (TEM) micrographs of freeze fractured liposome/GP aggregates reveal that the addition of the glycoprotein to liposomes led to the formation of a film (polymer adsorbed onto the bilayers) that tightly coated PC bilayers. The complete coating was already achieved at a PC : GP weight ratio of about 9:1. Image analysis profiles of digitalized TEM micrographs (PC : GP weight ratio 8:2) show that this film was formed by a multilayer structure. The periods of the average distance of the pattern ordering in layer structures (9-10 layers) were of about 2-3 nm and the thickness of the complete film was of about 25 nm. Higher amounts of glycoprotein resulted in a growth of this film, which exhibited at the highest proportion of this compound (50% in weight) a multifilm structure. An increasing resistance of liposomes to be affected by dodecyl maltoside both at subsolubilizing and solubilizing levels occurred as the proportion of the glycoprotein in the system rose, although this protective effect was more effective at low proportions of this compound (PC : GP weight ratios from 9:1 to 8:2). Thus, although a direct dependence was found between the growth of the enveloping structure and the resistance of the coated liposomes to be affected by the surfactant, the more effective protection occurred when this structure was a thin film formed by the assembly of various layers of GP of about 2-3 nm. Copyright 1999 John Wiley & Sons, Inc.

journal_name

Biopolymers

journal_title

Biopolymers

authors

de La Maza A,Codech L,Lopez O,Parra JL,Sabes M,Guinea J

doi

10.1002/(SICI)1097-0282(199911)50:6<579::AID-BIP2>

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

579-88

issue

6

eissn

0006-3525

issn

1097-0282

pii

10.1002/(SICI)1097-0282(199911)50:6<579::AID-BIP2>

journal_volume

50

pub_type

杂志文章
  • Structure and chain conformation of beta-glucan isolated from Auricularia auricula-judae.

    abstract::From Auricularia auricula-judae, a water soluble beta-D-glucan, named as AAG, was isolated by extraction with 70% ethanol/water solution. Its chemical structure was analyzed by gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), matrix-assisted laser desorption /ionization (MALDI)-time of flight (TO...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.20971

    authors: Ma Z,Wang J,Zhang L

    更新日期:2008-07-01 00:00:00

  • Collagen oligomers modulate physical and biological properties of three-dimensional self-assembled matrices.

    abstract::Elucidation of mechanisms underlying collagen fibril assembly and matrix-induced guidance of cell fate will contribute to the design and expanded use of this biopolymer for research and clinical applications. Here, we define how Type I collagen oligomers affect in-vitro polymerization kinetics as well as fibril micros...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.21537

    authors: Bailey JL,Critser PJ,Whittington C,Kuske JL,Yoder MC,Voytik-Harbin SL

    更新日期:2011-02-01 00:00:00

  • Interaction of anticancer drug cisplatin with guanine: density functional theory and surface-enhanced Raman spectroscopy study.

    abstract::Surface-enhanced Raman spectroscopy (SERS) in a silver sol assisted by density functional theory (DFT) calculations is shown to be a promising tool in the characterization of platinum complexes and their interaction with nucleic acid bases. This is demonstrated using cisplatin and guanine as a model. The energies and ...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.10480

    authors: Giese B,McNaughton D

    更新日期:2003-01-01 00:00:00

  • Secondary structure of a core protein from pig skin proteodermatan sulfate: CD and Fourier transform IR spectroscopic studies in solution.

    abstract::The secondary structure of a 38 kDa core protein from pig skin proteodermatan sulfate (PDS), was investigated in solution using CD and Fourier transform (FT) ir spectroscopy. Both techniques generally have provided complementary data on the secondary structures of proteins. CD spectral analysis has shown that the core...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.360281109

    authors: Renugopalakrishnan V,Damle SP,Horowitz PM,Moore S,Hutson TB,Gregory JD

    更新日期:1989-11-01 00:00:00

  • Method for generation of peptide-specific IgY antibodies directed to Staphylococcus aureus extracellular fibrinogen binding protein epitope.

    abstract::The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococc...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.22695

    authors: Walczak M,Grzywa R,Łupicka-Słowik A,Skoreński M,Bobrek K,Nowak D,Boivin S,Brown EL,Oleksyszyn J,Sieńczyk M

    更新日期:2015-09-01 00:00:00

  • Influence of structure of human, rat, and bovine serum albumins on binding properties of photoactive drug hypericin.

    abstract::Fluorescence binding measurements and molecular modeling were employed to study the interaction of hypericin (Hyp) with human (HSA), rat (RSA), and bovine (BSA) serum albumins. Fluorescence emission data show the solubility of Hyp increasing in the order BSA, HSA, and RSA. Molecular modeling was used to construct the ...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.10110

    authors: Hritz J,Kascakova S,Ulicny J,Miskovsky P

    更新日期:2002-01-01 00:00:00

  • S curve, a graphic representation of protein secondary structure sequence and its applications.

    abstract::A secondary structure sequence is a symbolic string composed of three kinds of letters, indicating the helix, strand, and coil (including turns), respectively. A graphic representation for this abstract symbolic sequence is proposed here, called the S curve. The S curve is the unique representation for a given seconda...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0282(200006)53:7<539::AID-BIP2>

    authors: Zhang CT,Zhang R

    更新日期:2000-06-01 00:00:00

  • Importance of the spatial display of charged residues in heparin-peptide interactions.

    abstract::Many studies have examined consensus sequences required for protein-glycosaminoglycan interactions. Through the synthesis of helical heparin binding peptides, this study probes the relationship between spatial arrangement of positive charge and heparin binding affinity. Peptides with a linear distribution of positive ...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.21339

    authors: Rullo A,Nitz M

    更新日期:2010-03-01 00:00:00

  • Crystal structure of cyclic (APGVGV)2, an analog of elastin, and a suggested mechanism for elongation/contraction of the molecule.

    abstract::Tropoelastin is a complex polymeric protein composed primarily of repeating segments of Val-Pro-Gly-Gly, Val-Pro-Gly-Val-Gly, and Ala-Pro-Gly-Val-Gly-Val that occurs in connective tissue and arteries. It has rubber-like extensible properties. A synthetic cyclic dodecapeptide, with a double repeat of the hexapeptide se...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.20214

    authors: Karle IL,Urry DW

    更新日期:2005-03-01 00:00:00

  • Alternative packing modes leading to amyloid polymorphism in five fragments studied with molecular dynamics.

    abstract::Amyloid aggregates have been implicated in the pathogenesis of diseases such as type 2 diabetes, Alzheimer's, Parkinson's, and prion disease. Recently determined microcrystal structures of several short peptide segments derived from fibril-forming proteins revealed coexistence of alternative aggregation modes (amyloid...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.21731

    authors: Berhanu WM,Masunov AE

    更新日期:2012-01-01 00:00:00

  • Enhanced deoxyribozyme-catalyzed RNA ligation in the presence of organic cosolvents.

    abstract::Deoxyribozyme and aptamer selections are typically conducted in aqueous buffer solutions. Using nonaqueous cosolvents in selection experiments will help expand the activity of deoxyribozymes with non-oligonucleotide substrates and will allow identification of new aptamers for nonprotein targets. We undertook in vitro ...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.22191

    authors: Behera AK,Schlund KJ,Mason AJ,Alila KO,Han M,Grout RL,Baum DA

    更新日期:2013-06-01 00:00:00

  • Diverse cyclic seed peptides in the Mexican zinnia (Zinnia haageana).

    abstract::A new family of small plant peptides was recently described and found to be widespread throughout the Millereae and Heliantheae tribes of the sunflower family Asteraceae. These peptides originate from the post-translational processing of unusual seed-storage albumin genes, and have been termed PawS-derived peptides (P...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.22901

    authors: Franke B,Jayasena AS,Fisher MF,Swedberg JE,Taylor NL,Mylne JS,Rosengren KJ

    更新日期:2016-11-01 00:00:00

  • Optimizing the fold stability of the circularly permuted Trp-cage motif.

    abstract::Through optimization of the linker region and key stabilizing mutations, it has been possible to improve the stability of the circularly permuted (cp) Trp-cage miniprotein. However, even the most stable Trp-cage circular permutants are still less stable than the analogous standard topology (std) Trp-cages. Extending m...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.23327

    authors: Graham KA,Byrne A,Mason M,Andersen NH

    更新日期:2019-12-01 00:00:00

  • Multinuclear NMR and FTIR studies of new polyoxaalkyl esters of lasalocid and their complexes with lithium and sodium cations.

    abstract::Three new polyoxaalkyl esters of lasalocid are synthesized. Their ability to form complexes with Li(+) and Na(+) cations is studied using multinuclear NMR methods, FTIR spectroscopy in the middle and far IR regions, and mass spectrometry. It is found that lasalocid esters form only 1:1 complexes with the metal cations...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.10194

    authors: Pankiewicz R,Schroeder G,Gierczyk B,Brzezinski B,Bartl F

    更新日期:2002-10-15 00:00:00

  • Association behavior of native beta-lactoglobulin.

    abstract::The association behavior of beta-lactoglobulin has been studied by small-angle neutron scattering as a function of protein concentration, temperature, pH, and NaCl concentration of the solution. By indirect Fourier transformation of the spectra, pair-distance distribution functions for the various samples were obtaine...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0282(199901)49:1<11::AID-BIP2>3

    authors: Verheul M,Pedersen JS,Roefss SP,de Kruif KG

    更新日期:1999-01-01 00:00:00

  • Oxidative folding of hepcidin at acidic pH.

    abstract::Hepcidin is a four disulfide 25-residue peptide hormone which has a central role in the regulation of iron homeostasis. To support studies on hepcidin we have sought to establish reliable and robust synthetic methods for the preparation of correctly folded materials. While correctly-folded hepcidin has good aqueous so...

    journal_title:Biopolymers

    pub_type: 杂志文章,评审

    doi:10.1002/bip.21383

    authors: Zhang J,Diamond S,Arvedson T,Sasu BJ,Miranda LP

    更新日期:2010-01-01 00:00:00

  • A minimalistic 3D pharmacophore model for cyclopentapeptide CXCR4 antagonists.

    abstract::Because of its involvement in HIV entry, the chemokine receptor CXCR4 is an attractive target for antiretroviral drugs. Despite the large number of CXCR4 inhibitors studied, the 3D pharmacophore for binding to CXCR4 remains elusive, mainly as a result of conformational flexibility inherent in the identified ligands. I...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.20508

    authors: Våbenø J,Nikiforovich GV,Marshall GR

    更新日期:2006-01-01 00:00:00

  • Isolation, characterization, and magnesium-induced self-association kinetics of discrete aggregates of RecA protein from Escherichia coli.

    abstract::Dynamic and static intensity light scattering techniques were employed to identify conditions allowing preparation of homogeneous solutions of distinct oligomeric states of RecA protein. These hydrodynamically distinguishable oligomer populations of RecA protein were obtained in homogeneous pure quantities sufficient ...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0282(199604)38:4<471::aid-bip4>

    authors: Budzynski DM,Gao X,Benight AS

    更新日期:1996-04-01 00:00:00

  • Osmolyte-induced changes in protein conformational equilibria.

    abstract::Examining solute-induced changes in protein conformational equilibria is a long-standing method for probing the role of water in maintaining protein stability. Interpreting the molecular details governing the solute-induced effects, however, remains controversial. We present experimental and theoretical data for osmol...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0282(20000405)53:4<293::AID-BIP

    authors: Saunders AJ,Davis-Searles PR,Allen DL,Pielak GJ,Erie DA

    更新日期:2000-04-05 00:00:00

  • Keratin-lipid structural organization in the corneous layer of snake.

    abstract::The shed epidermis (molt) of snakes comprises four distinct layers. The upper two layers, here considered as beta-layer, contain essentially beta-keratin. The following layer, known as mesos-layer, is similar to the human stratum corneum, and is formed by thin cells surrounded by intercellular lipids. The latter layer...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.21184

    authors: Ripamonti A,Alibardi L,Falini G,Fermani S,Gazzano M

    更新日期:2009-12-01 00:00:00

  • Total chemical synthesis and oxidative folding of delta-conotoxin PVIA containing an N-terminal propeptide.

    abstract::Small disulfide-rich peptides are translated as larger precursors typically containing an N-terminal prepro sequence. In this study, we investigated the role of a propeptide in the oxidative folding of an extremely hydrophobic delta-conotoxin, PVIA. delta-Conotoxin PVIA (delta-PVIA) is a 29-amino acid neurotoxin stabi...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.20211

    authors: Buczek P,Buczek O,Bulaj G

    更新日期:2005-01-01 00:00:00

  • Phosphorylation stabilizes the N-termini of alpha-helices.

    abstract::The role of phosphorylation in stabilizing the N-termini of alpha-helices is examined using computer simulations of model peptides. The models comprise either a phosphorylated or unphosphorylated serine at the helix N-terminus, followed by nine alanines. Monte Carlo/stochastic Dynamics simulations were performed on th...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0282(199903)49:3<225::AID-BIP4>

    authors: Smart JL,McCammon JA

    更新日期:1999-03-01 00:00:00

  • Challenges in mass spectrometry-based quantification of bioactive peptides: a case study exploring the neuropeptide Y family.

    abstract::The study of biologically active peptides is critical to the understanding of physiological pathways, especially those involved in the development of disease. Historically, the measurement of biologically active endogenous peptides has been undertaken by radioimmunoassay, a highly sensitive and robust technique that p...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.22109

    authors: Xi L,Jin Y,Parker EA,Josh P,Jones A,Wijffels G,Colgrave ML

    更新日期:2012-01-01 00:00:00

  • Conformational studies on a synthetic C-terminal fragment of the alpha subunit of G(S) proteins.

    abstract::It has recently been reported that synthetic peptides corresponding to the C-terminal sequence of G alpha, can be used to study the molecular mechanisms of interaction between this protein and G protein coupled receptors (Hamm et al., Science, 1988, Vol. 241, pp. 832-835). A conformational analysis on a 11 amino acids...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/1097-0282(200009)54:3<186::AID-BIP50>3.0.C

    authors: Albrizio S,D'Ursi A,Fattorusso C,Galoppini C,Greco G,Mazzoni MR,Novellino E,Rovero P

    更新日期:2000-09-01 00:00:00

  • Effects of the minor groove pyrimidine nucleobase functional groups on the stability of duplex DNA: the impact of uncompensated minor groove amino groups.

    abstract::DNA sequences containing four types of analog nucleosides are described. All four are pyridine derivatives constructed as C-nucleosides so that they mimic the pyrimidine derivatives 2'-deoxyuridine, thymidine or 2'-deoxycytidine, but in all cases the analogs lack the corresponding O2-carbonyls that in duplex DNA are l...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.20817

    authors: Sun Z,McLaughlin LW

    更新日期:2007-10-05 00:00:00

  • The TIPP opioid peptide family: development of delta antagonists, delta agonists, and mixed mu agonist/delta antagonists.

    abstract::The discovery of the prototype delta opioid antagonists TIPP (H-Tyr-Tic-Phe-Phe-OH) and TIP (H-Tyr-Tic-Phe-OH) in 1992 was followed by extensive structure-activity relationship studies, leading to the development of analogues that are of interest as pharmacological tools or as potential therapeutic agents. Stable TIPP...

    journal_title:Biopolymers

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0282(1999)51:6<411::AID-BIP4>3.

    authors: Schiller PW,Weltrowska G,Berezowska I,Nguyen TM,Wilkes BC,Lemieux C,Chung NN

    更新日期:1999-01-01 00:00:00

  • Solid-supported and pegylated H-Pro-Pro-Asp-NHR as catalysts for asymmetric aldol reactions.

    abstract::H-Pro-Pro-Asp-NH2 is a highly active and selective catalyst for asymmetric aldol reactions. Here, the versatility of H-Pro-Pro-Asp-NH2 has been further improved by immobilization on a solid support and functionalization with a short polyethylene glycol linker at the C-terminus. The development, synthesis, and the cata...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.20393

    authors: Revell JD,Gantenbein D,Krattiger P,Wennemers H

    更新日期:2006-01-01 00:00:00

  • Fast membrane association is a crucial factor in the peptide pep-1 translocation mechanism: a kinetic study followed by surface plasmon resonance.

    abstract::The use of peptide carriers, termed "cell-penetrating peptides (CPPs)" has attracted much attention due to their potential for cellular delivery of hydrophilic molecules with pharmacological interest, overcoming the membrane barrier. These peptides are able to deliver attached cargos in a nontoxic manner, with the upt...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.21367

    authors: Henriques ST,Castanho MA,Pattenden LK,Aguilar MI

    更新日期:2010-01-01 00:00:00

  • Review: Progresses in understanding N-ethylmaleimide sensitive factor (NSF) mediated disassembly of SNARE complexes.

    abstract::N-ethylmaleimide sensitive factor (NSF) is a key protein of intracellular membrane traffic. NSF is a highly conserved protein belonging to the ATPases associated with other activities (AAA+ proteins). AAA+ share common domains and all transduce ATP hydrolysis into major conformational movements that are used to carry ...

    journal_title:Biopolymers

    pub_type: 杂志文章,评审

    doi:10.1002/bip.22854

    authors: Ryu JK,Jahn R,Yoon TY

    更新日期:2016-08-01 00:00:00

  • Biasing a Monte Carlo chain growth method with Ramachandran's plot: application to twenty-L-alanine.

    abstract::In this paper, we explore the possibility of using experimental observations in the Monte Carlo chain growth method that we have previously developed. In this method, the macromolecule (peptide, protein, nuclei acid, etc.) is grown atom-by-atom (or residue-by-residue, etc.) and partial chains are replicated according ...

    journal_title:Biopolymers

    pub_type: 杂志文章

    doi:10.1002/bip.360331210

    authors: Bascle J,Garel T,Orland H,Velikson B

    更新日期:1993-12-01 00:00:00