Abstract：
:Bronchial subepithelial fibrosis is an histological characteristic of asthma. Cytokines and other mediators, such as PDGF-BB, TGF-beta1 and ET-1 found in the asthmatic submucosa can potentially activate a repair process that leads to fibroblast proliferation and collagen synthesis. The mechanisms of modulation of the repair process leading to extracellular matrix deposition are still to be documented. In this study, we assessed the in vitro proliferation and collagen synthesis of bronchial fibroblasts isolated from normal and asthmatic subjects in response to ET-1, platelet-derived growth factor (PDGF)-BB and transforming growth factor (TGF)-beta1 alone or in combination, in the presence or absence of dexamethasone. The combination of ET-1 with one of the other two growth factors, or the triple combination, significantly increased DNA synthesis and collagen production of bronchial fibroblasts isolated from both normal and asthmatic subjects, but the same growth factors used separately had no significant effect on the same parameters. These results suggest that the simultaneous presence of ET-1, PDGF-BB and TGF-beta1 in both normal and asthmatic subjects is necessary to activate bronchial fibroblast proliferation and collagen synthesis. As these mediators are present in the submucosa of the asthmatic bronchi, they could be responsible, at least in part, for the accumulation of collagen in the mucosa.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Dubé J,Chakir J,Dubé C,Grimard Y,Laviolette M,Boulet LPdoi
10.1046/j.1365-2613.2000.00173.xsubject
Has Abstractpub_date
2000-12-01 00:00:00pages
429-37issue
6eissn
0959-9673issn
1365-2613pii
iep173journal_volume
81pub_type
杂志文章abstract：:One of the consequences of neoplastic transformation is deregulation of tissue collagen metabolism. Although metalloproteinases initiate the breakdown of collagen in lung carcinoma, the final step of collagen degradation is mediated by prolidase (E.C.3.4.13.9). We investigated whether prolidase activity could reflect ...
journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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abstract：:Diets enriched with fat, especially unsaturated fat, promote experimental pancreatic carcinogenesis, but little is known of the effects of individual fatty acids. The effect of stearic and oleic acid on pancreatic fatty acids and atypical acinar cell nodules (preneoplastic lesions) was studied in 14-day-old weanling m...
journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
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journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2613.2007.00537.x
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2001.00202.x
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2009.00692.x
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2000.00150.x
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journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
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journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2613.2001.iep0082-0149-x
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
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doi：
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
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journal_title：International journal of experimental pathology
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journal_title：International journal of experimental pathology
pub_type： 杂志文章
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