Abstract：
:Intratracheal instillation (IT) of bleomycin is a widely used experimental model for lung fibrosis. In this study we describe the time-course of bleomycin-induced lung fibrosis in mice using computer-assisted morphometry. C57Bl/6J mice were treated with a single IT dose of bleomycin or control saline. Animals were killed 3, 6, 14 and 21 days post-IT. Lung injury was evaluated by analysis of bronchoalveolar lavage (BAL) fluid, hydroxyproline concentration in the lung, routine light microscopic examination resulting in a semiquantitative morphological index (SMI) of lung injury, and quantitative morphological measurements (fibrosis fraction and alveolar wall area fraction) aided by optimas image analysis software. Changes in BAL fluid attributed to bleomycin treatment include increased total cell count (days 14 and 21), and increased percentage of neutrophils (days 3 and 6) followed by a sustained increase in lymphocytes (days 6, 14 and 21). Hydroxyproline levels increased in bleomycin-treated mice on days 14 and 21. Median SMI grades were significantly elevated on days 3, 14 and 21. Computer-assisted morphometry demonstrated a 3-fold increase in fibrosis fraction and a 1.3-fold increase in wall area fraction in bleomycin-treated mice on day 14, with no further increase on day 21. These data also demonstrate that the most suitable time point for assessing lung fibrosis in this model is 14 days after IT instillation of bleomycin, based on the observation that at 14 days the animals developed extensive fibrosis, but had less variability in the fibrotic response and lower mortality than later at 21 days. Computer-assisted morphometry provides objective and quantitative measurements that are a useful tool for the evaluation of bleomycin-induced lung injury.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Izbicki G,Segel MJ,Christensen TG,Conner MW,Breuer Rdoi
10.1046/j.1365-2613.2002.00220.xsubject
Has Abstractpub_date
2002-06-01 00:00:00pages
111-9issue
3eissn
0959-9673issn
1365-2613pii
220journal_volume
83pub_type
杂志文章abstract：:p23 is a cochaperone of heat shock protein 90 and also interacts functionally with numerous steroid receptors and kinases. However, the in vivo roles of p23 remain unclear. To explore its in vivo function, we generated the transgenic (TG) mice ubiquitously overexpressing p23. The p23 TG mice spontaneously developed ki...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2011.00762.x
更新日期：2011-08-01 00:00:00
abstract：:Polymorphonuclear cells (PMN) are believed to be important effector cells responsible the myocardial damage seen following ischaemia. However, the exact kinetics of their migration remains controversial. Isolated PMN (10 x 10(6) cells) labelled with (51)Cr were injected into four groups of Lewis rats: 0 h (T0h; n = 13...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2007.00541.x
更新日期：2007-08-01 00:00:00
abstract：:Lysyl oxidase, which cross-links collagen and elastin, was obtained from chick embryo bone and cartilage and its substrate, elastin, from aorta. The enzyme was studied using an improved assay which enabled the stability of the substrate to be monitored. The enzyme was fully inhibited in vivo by beta-aminopropionitrile...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:Low-dose chemotherapy drugs can suppress tumours by restraining tumour vessel growth and preventing the repair of damaged vascular endothelial cells. Cisplatin is a broad-spectrum, cell cycle-non-specific drug, but has serious side effects if used at high doses. There have been few reports on the anti-angiogenic effec...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2009.00684.x
更新日期：2010-02-01 00:00:00
abstract：:Up to 40% of children with Reye's syndrome have hypoglycaemia that could contribute to the patient's encephalopathy. We developed a mouse model in which intravenous inoculation of influenza B/Lee virus produced a non-permissive infection of hepatocytes and cerebral endothelial cells and caused many clinical, biochemic...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:Regression of submandibular acinar cell hyperplasia after withdrawal of the mitogenic stimulus induced by isoprenaline was studied in male Wistar rats. Intraperitoneal administration of 39 mg of isoprenaline, in divided doses over a 9-day period, resulted in a marked increase in gland size and weight. The proportional...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:The degenerative and regenerative changes in murine skeletal muscle after injection of Bothrops asper venom were studied by histological, lectin histochemical and immunocytochemical techniques. According to our observations, the process was divided into four main stages: (a) During the first 3 days prominent degenerat...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The interrelationship between acinar cell apoptosis and tubular complex formation was examined in caerulein-induced pancreatitis using histology, immunohistochemistry, electron microscopy and DNA gel electrophoresis. Rats were given 8 hourly subcutaneous injections of caerulein, 24 micrograms/kg, for up to 2 days. Mor...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1999.00116.x
更新日期：1999-08-01 00:00:00
abstract：:Permethrin (PM), a synthetic pyrethroid insecticide, has broad toxicity spectra. We aimed to investigate the effects of PM on the testes of adult albino rats, examine the recovery response and evaluate the efficacy of naringenin (NG) supplementation. Adult male albino rats were randomly assigned to five groups of six ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12168
更新日期：2016-02-01 00:00:00
abstract：:The acute phase of experimental Chagas' disease in rats is associated with early lesions of the post-ganglionic sympathetic nerve terminals of the heart, the varicosities being the target. In the superior cervical and stellate ganglia the preganglionic fibres showed no signs of lesion in the course of experimental Cha...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1996.00971.x
更新日期：1996-06-01 00:00:00
abstract：:Dengue type 2 virus (DV) induces a subpopulation of T lymphocytes of mouse spleen to secrete a soluble helper cytokine (HF) which enhances the DV-specific IgM antibody plaque forming cells (PFC). The present study undertaken to purify and characterize HF shows that it can be purified by low pressure liquid chromatogra...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:BALB/c and CBA mice maintained on low (4%) or normal (18%) protein diets for 2 weeks after weaning were infected with a sublethal dose of murine cytomegalovirus, adjusted in proportion to body weight. Viral replication, histopathological changes and humoral responses to the virus were compared between the dietary grou...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:The use of non-specific inhibitors of tissue non-specific alkaline phosphatase (TNSALP) in pre-adipocytes blocks intracellular lipid accumulation. TNSALP is also expressed in hepatocytes, which are known to accumulate lipid in a similar manner to pre-adipocytes. The purpose of this study was to use specific silencing ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12243
更新日期：2017-10-01 00:00:00
abstract：:(Pre)neoplastic lesions in livers of rats induced by diethylnitrosamine are characterized by elevated activity of the first irreversible enzyme of the oxidative branch of the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PD), for production of NADPH. In the present study, the activity of G6PD, ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2008.00582.x
更新日期：2008-08-01 00:00:00
abstract：:In order to clarify the function of hepatocyte growth factor (HGF) in vivo, we have developed transgenic mice expressing HGF in the liver. The bromodeoxyuridine labelling indices in livers from HGF transgenic mice were doubled, compared to those from wild type mice. Livers of HGF transgenic mice expressed high levels ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2613.1998.730403.x
更新日期：1998-10-01 00:00:00
abstract：:C-cell tumours of the thyroid gland are among the most common spontaneous neoplasms of the laboratory rat. With the exception of calcitonin, little attention has been paid to the secretory peptides of C cells during the development of neoplasia. Of these peptides, somatostatin (SS) is of particular interest because it...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.0959-9673.2004.00367.x
更新日期：2004-02-01 00:00:00
abstract：:The aim of the present study was to evaluate the coexpression of very early (CD69), early (CD25) and late (HLADR) antigens and to analyse the mean fluorescence intensity (MFI) of such activation markers on synovial fluid (SF) and peripheral blood (PB) lymphocytes of patients affected by rheumatoid arthritis (RA) and o...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1997.290360.x
更新日期：1997-10-01 00:00:00
abstract：:Although it is well known that cadmium (Cd) causes adverse effects on male rat reproductive organs, few studies have quantified alterations caused by its low doses. Quantification of these alterations, especially in the testis, was measured using morphometry. A single dose of cadmium chloride (1 or 1.2 mg/kg BW) was i...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2009.00692.x
更新日期：2010-04-01 00:00:00
abstract：:Pathological and immunopathological studies were carried out on snake coiled fibres (SCF) which occurred in affected soleus muscle in chloroquine treated rats. The SCF began to appear in denervated soleus muscle by 8 days after chloroquine injection. By day 14, typical SCF were observed with an unusual swirling patter...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1997.9950333.x
更新日期：1997-02-01 00:00:00
abstract：:Angiogenesis is a common finding in chronic inflammatory diseases; however, its role in Sjögren's syndrome (SS) remains to be elucidated. Previous SS studies have demonstrated an increase in VEGF-A/VEGFR-2 system expression in minor salivary gland (MSG) biopsies from patients with SS, but differences in the new blood ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12061
更新日期：2014-04-01 00:00:00
abstract：:In this study, the extent of angiogenesis, evaluated as microvascular volume density, immunoreactivity of tumour cells to erythropoietin (Epo) and of endothelial cells to Epo receptor (EpoR) have been correlated in human primary melanoma specimens. Results showed that Epo/EpoR expression correlate with angiogenesis an...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2010.00731.x
更新日期：2010-12-01 00:00:00
abstract：:Cyclophilins are proteins which are remarkably conserved through evolution; moreover they have been found in every possible existing organism, which indicates their fundamental importance. Due to their enzymatic properties, multiplicity, cellular localization and role in protein folding they belong to the group of pro...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2613.1999.00128.x
更新日期：1999-12-01 00:00:00
abstract：:Progress in understanding the pathophysiology of abdominal aortic aneurysms (AAA) is dependent in part on the development and application of effective animal models that recapitulate key aspects of the disease. The objective was to produce an experimental model of AAA in rats by combining two potential causes of metal...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2010.00746.x
更新日期：2011-02-01 00:00:00
abstract：:Pterygium is a surface ocular lesion that is associated with chronic UV exposure. The primary effect is a solar actinic elastosis within the stroma. All the other changes are secondary. Pterygium is characterized by proliferation, inflammatory infiltrates, fibrosis, angiogenesis and extracellular matrix breakdown. The...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2009.00665.x
更新日期：2009-12-01 00:00:00
abstract：:Initially found expressed in neuronal and then later in endothelial cells, it is well established that the transmembrane glycoproteins neuropilin-1 (NRP1) and neuropilin-2 (NRP2) play essential roles in axonal growth and guidance and in physiological and pathological angiogenesis. Neuropilin expression and function in...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2613.2012.00810.x
更新日期：2012-04-01 00:00:00
abstract：:Haptoglobin is an acute phase protein known to be highly expressed in the liver. Recently, we showed increased local arterial haptoglobin expression after flow-induced arterial remodelling and found that haptoglobin is involved in cell migration and arterial restructuring probably through accumulation of a temporary g...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2003.00336.x
更新日期：2003-04-01 00:00:00
abstract：:The aim of this study was to estimate the relationship of endothelial dysfunction induced by intracellular S-adenosylhomocysteine (SAH) accumulation and DNA methylation in human umbilical vein endothelial cells (HUVEC). The isolated HUVEC were incubated with 3-deazaadenosine (DZA) to induce experimental intracellular ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2009.00687.x
更新日期：2009-12-01 00:00:00
abstract：:The purpose of this study was to examine the prognostic significance of insulin-like growth factor-1 receptor (IGF-1R) expression alone and in relation to the expression of the MET- receptor and the MET-homologous receptor RON, in classical Hodgkin's lymphoma (cHL). Tumour samples from patients with cHL (n = 202; medi...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12128
更新日期：2015-08-01 00:00:00
abstract：:The relationship between donor age and the growth characteristics of normal human smooth muscle cell cultures derived from aorta and stomach was examined in a series of growth studies. A negative correlation was demonstrated between donor age and growth potential of both cell types in primary culture. This decreased g...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:One of the consequences of neoplastic transformation is deregulation of tissue collagen metabolism. Although metalloproteinases initiate the breakdown of collagen in lung carcinoma, the final step of collagen degradation is mediated by prolidase (E.C.3.4.13.9). We investigated whether prolidase activity could reflect ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2000.00168.x
更新日期：2000-10-01 00:00:00
