Abstract：
:Tumour-associated macrophage (TAM) polarization is associated with hepatocellular carcinoma but the molecular mechanism of this polarization is still unknown. Peripheral blood mononuclear cells were induced to differentiate into M0, M1 and M2 macrophages and TAMs. TAMs were transfected with pcDNA3.1-GAS5, pcDNA3.1-NC, si-GAS5, si-PTEN or si-Ctrl. A human liver cancer cell line (SMCC-7721) was incubated with the modified TAM supernatant. Quantitative real-time PCR and Western blot were performed to detect gene and protein expression. The cell proliferation and invasion properties of the SMCC-7721 cells were detected by MTT and Transwell assays. GAS5 is up-regulated in M1 macrophages and down-regulated in M2 macrophages and TAMs. GAS5 overexpression promoted M1-like polarization of TAMs and inhibited M2-like polarization of TAMs. Moreover, GAS5 promoted the expression of PTEN in TAMs. PTEN-silenced TAM supernatant treatment promoted cell proliferative and invasive properties of the SMCC-7721 cells and diminished the effect of GAS5-overexpressed TAM supernatant on the cell proliferation and invasion by SMCC-7721 cells. Our results demostrared that GAS5 overexpression inhibited M2-like polarization of TAMs by enhancing PTEN expression, thereby inhibiting cell proliferation and invasion by SMCC-7721 cells. Thus, our results suggest that GAS5 may be a new therapeutic target for HCC treatment.
journal_name
Int J Exp Patholjournal_title
International journal of experimental pathologyauthors
Wang X,Li FY,Zhao W,Gao ZK,Shen B,Xu H,Cui YFdoi
10.1111/iep.12374subject
Has Abstractpub_date
2020-12-01 00:00:00pages
215-222issue
6eissn
0959-9673issn
1365-2613journal_volume
101pub_type
杂志文章abstract：:Groups of mice were given oestrone acetate or vitamin E subcutaneously to determine how these treatments might modify responses to endotoxic lipopolysaccharide given intraperitoneally. Release of hepatic transaminase and tumour necrosis factor (TNF) into serum and induction of manganous superoxide dismutase in the liv...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:In chronic administration of sodium valproate to rats, significant disorders of structural integrity of the hippocampal gyrus and the neocortex of the temporal lobe, observed in the last two stages of the experiment (after 9 and 12 months), coexisted with increased number of microglial cells and, especially after 12 m...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.0959-9673.2005.00417.x
更新日期：2005-04-01 00:00:00
abstract：:Using uptake of the fluorescent bile acid derivative cholylglycylamido-fluorescein (FITC-GC) as a measurement of liver cell population size and function, the antiproliferative and toxic effects of the well known cytostatic drug, cisplatin was evaluated on rapidly growing rat hepatoma McA-RH7777 cells and rat hepatocyt...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1998.00054.x
更新日期：1998-04-01 00:00:00
abstract：:Although the exact mechanism for the progression of myelofibrosis in acute megakaryoblastic leukaemia is unclear, certain humoral factors released from the proliferating megakaryoblasts that are unable to store these factors in their defective alpha-granules, including platelet derived growth factor (PDGF), fibroblast...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:The acute phase of experimental Chagas' disease in rats is associated with early lesions of the post-ganglionic sympathetic nerve terminals of the heart, the varicosities being the target. In the superior cervical and stellate ganglia the preganglionic fibres showed no signs of lesion in the course of experimental Cha...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1996.00971.x
更新日期：1996-06-01 00:00:00
abstract：:Systemic hypertension, characterized by elevated blood pressure ≥140/90 mm Hg, is a major modifiable risk factor for cardiovascular disease. Hypertension also associates with non-alcoholic fatty liver disease (NAFLD), which is becoming common due to a modern diet and lifestyle. The aim of the present study was to exam...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12369
更新日期：2020-10-01 00:00:00
abstract：:The relationship between donor age and the growth characteristics of normal human smooth muscle cell cultures derived from aorta and stomach was examined in a series of growth studies. A negative correlation was demonstrated between donor age and growth potential of both cell types in primary culture. This decreased g...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Polymorphonuclear cells (PMN) are believed to be important effector cells responsible the myocardial damage seen following ischaemia. However, the exact kinetics of their migration remains controversial. Isolated PMN (10 x 10(6) cells) labelled with (51)Cr were injected into four groups of Lewis rats: 0 h (T0h; n = 13...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2007.00541.x
更新日期：2007-08-01 00:00:00
abstract：:An important means of determining how amyloid-beta peptide (Abeta) affects cells is to identify specific macromolecular targets and assess how Abeta interaction with such targets impacts on cellular functions. On the one hand, cell surface receptors interacting with extracellular Abeta have been identified, and their ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1111/j.0959-9673.2005.00427.x
更新日期：2005-06-01 00:00:00
abstract：:Non-alcoholic fatty liver disease (NAFLD) is the most common chronic condition of the liver in the western world. There is only little evidence about altered sensitivity of steatotic liver to acute toxic injury. The aim of this project was to test whether hepatic steatosis sensitizes rat liver to acute toxic injury in...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2011.00765.x
更新日期：2011-08-01 00:00:00
abstract：:Intratracheal instillation (IT) of bleomycin is a widely used experimental model for lung fibrosis. In this study we describe the time-course of bleomycin-induced lung fibrosis in mice using computer-assisted morphometry. C57Bl/6J mice were treated with a single IT dose of bleomycin or control saline. Animals were kil...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2002.00220.x
更新日期：2002-06-01 00:00:00
abstract：:The pathogenesis of dengue haemorrhagic fever (DHF) is incompletely understood but it has been suggested that various cytokines may have a role in the process. In this study the profile of the cytokine Transforming Growth Factor-beta 1 (TGF-beta1) was investigated in the sera of 79 patients with various grades of deng...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1999.00107.x
更新日期：1999-06-01 00:00:00
abstract：:This study aimed to characterize matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in relation to changes in left ventricle (LV) geometry and function in a porcine model with streptozotocin (STZ)-induced diabetes. In 15 Chinese Guizhou minipigs with STZ-induced diabetes (diabetic group) and 15 age-m...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2008.00579.x
更新日期：2008-04-01 00:00:00
abstract：:Mitochondrial DNA (mt-DNA) disorders and abnormal regulation of nuclear-derived proteins devoted to the cross-talk between the two cellular genomes have recently interested researchers in the field of neuromuscular diseases. We have identified, isolated and sequenced a new gene, augmenter of liver regeneration (ALR) t...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2009.00639.x
更新日期：2009-08-01 00:00:00
abstract：:In this study we have explored the pathogenesis of the hepatic alterations which occur in diabetes and the modulation of these complications by the combination of metformin adjunct treatment and insulin monotherapy. For this purpose, diabetic rats were treated with insulin (DM + Ins) or metformin plus insulin (DM + Me...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12184
更新日期：2016-06-01 00:00:00
abstract：:The aim of this study was to evaluate the use of flaxseed in animals subjected to ethanol-induced hepatotoxicity. Twenty-four male rats were divided into four groups (n = 6): control group (CG) which received a control diet and water ad libitum; flaxseed group (FG) which received control diet with an addition of 25% f...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12288
更新日期：2018-10-01 00:00:00
abstract：:The α2-adrenoceptors regulate the sympathetic nervous system, controlling presynaptic catecholamine release. However, the role of the α2-adrenoceptors in cutaneous wound healing is poorly understood. Mice lacking both the α2A/α2C-adrenoceptors were used to evaluate the participation of the α2-adrenoceptor during cutan...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12093
更新日期：2014-10-01 00:00:00
abstract：:The role of lymphocytes in the pathogenesis of lung fibrosis is not clear, but the weight of the evidence supports a pro-fibrotic effect for lymphocytes. The high-affinity interleukin-2 receptor (haIL-2R) is expressed on activated, but not quiescent, T lymphocytes. This selective expression of haIL-2R provides the bas...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.0959-9673.2005.00436.x
更新日期：2005-10-01 00:00:00
abstract：:Duchenne's muscular dystrophy (DMD) is a lethal childhood disease caused by mutations of the dystrophin gene, the protein product of which, dystrophin, has a vital role in maintaining muscle structure and function. Homologues of DMD have been identified in several animals including dogs, cats, mice, fish and invertebr...
journal_title：International journal of experimental pathology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2613.2003.00354.x
更新日期：2003-08-01 00:00:00
abstract：:Leprosy is a disease consisting of a spectrum of clinical, bacteriological, histopathological and immunological manifestations. Tuberculoid leprosy is frequently recognized as the benign polar form of the disease, while lepromatous leprosy is regarded as the malignant form. The different forms of leprosy depend on the...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12108
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abstract：:Pathological and immunopathological studies were carried out on snake coiled fibres (SCF) which occurred in affected soleus muscle in chloroquine treated rats. The SCF began to appear in denervated soleus muscle by 8 days after chloroquine injection. By day 14, typical SCF were observed with an unusual swirling patter...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1997.9950333.x
更新日期：1997-02-01 00:00:00
abstract：:A reversible impairment in the ability of the liver to secrete cholephilic compounds has been reported to exist in infant rats born from mothers with surgically induced complete cholestasis during the last third of the pregnancy. Canalicular plasma membranes (CPM) were purified from livers obtained from 4 and 8 week-o...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1997.360372.x
更新日期：1997-12-01 00:00:00
abstract：:Angiogenesis is a common finding in chronic inflammatory diseases; however, its role in Sjögren's syndrome (SS) remains to be elucidated. Previous SS studies have demonstrated an increase in VEGF-A/VEGFR-2 system expression in minor salivary gland (MSG) biopsies from patients with SS, but differences in the new blood ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/iep.12061
更新日期：2014-04-01 00:00:00
abstract：:Tumour growth is angiogenesis dependent. Thrombosis and thromboembolism are very common in cancer patients. These patients are often treated with heparin as an anti-coagulant. Many tumour angiogens, including VEGF165, and endogenous anti-angiogenesis factors bind heparin tightly. Using the non-surgical mesenteric-wind...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.2000.00150.x
更新日期：2000-06-01 00:00:00
abstract：:The roles of inducible nitric oxide synthase (iNOS) in the development and healing of gastric ulcers have not been fully characterized. We characterized iNOS expression in experimentally induced ulcers in rat and mouse stomachs and investigated the roles of iNOS using iNOS gene-deficient (iNOS-/-) mice and wildtype mi...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2003.00357.x
更新日期：2003-10-01 00:00:00
abstract：:The aim of this study was to estimate the relationship of endothelial dysfunction induced by intracellular S-adenosylhomocysteine (SAH) accumulation and DNA methylation in human umbilical vein endothelial cells (HUVEC). The isolated HUVEC were incubated with 3-deazaadenosine (DZA) to induce experimental intracellular ...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2009.00687.x
更新日期：2009-12-01 00:00:00
abstract：:BALB/c and CBA mice maintained on low (4%) or normal (18%) protein diets for 2 weeks after weaning were infected with a sublethal dose of murine cytomegalovirus, adjusted in proportion to body weight. Viral replication, histopathological changes and humoral responses to the virus were compared between the dietary grou...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:Plasmodium berghei ANKA (PbA) infection in susceptible inbred mouse strains is the most commonly used experimental model to study pathogenesis of cerebral malaria (CM). Indeed, many concepts on mechanisms related to this complication have arisen from works using this model. Although inbred strains present several adva...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2008.00622.x
更新日期：2009-04-01 00:00:00
abstract：:To confirm the existence of the cervical thymus and the development of cervical thymoma in thymoma-prone BUF/Mna (BUF) rats, we examined cervical organs and adjacent tissues, and thoracic thymic tissues of the three inbred strains, BUF, ACI/NMna (ACI), and WKY/NCrj (WKY), and 11 congenic strains, in which genetic regi...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1111/j.1365-2613.2010.00755.x
更新日期：2011-04-01 00:00:00
abstract：:The aim of the present study was to evaluate the coexpression of very early (CD69), early (CD25) and late (HLADR) antigens and to analyse the mean fluorescence intensity (MFI) of such activation markers on synovial fluid (SF) and peripheral blood (PB) lymphocytes of patients affected by rheumatoid arthritis (RA) and o...
journal_title：International journal of experimental pathology
pub_type： 杂志文章
doi：10.1046/j.1365-2613.1997.290360.x
更新日期：1997-10-01 00:00:00
