当前位置： SCI文献检索 > JOURNAL OF NEUROIMMUNOLOGY期刊下所有文献 > ATP and adenosine induce ramification of microglia in vitro.

ATP and adenosine induce ramification of microglia in vitro.

Abstract：

:Microglial cells in the healthy adult brain possess a characteristic ramified morphology with multiple branched processes, small somata and down-regulated inflammatory properties. In contrast, microglial cells isolated from new-born rat brain inevitably show a non-ramified amoeboid phenotype, which is observed in vivo after pathologic activation or during development. To identify factors that control microglial morphology we investigated the effects of purines alone or in combination with astrocyte-conditioned medium (ACM). Under optimized culture conditions postnatal rat microglial cells developed an amoeboid to ovoid phenotype. Addition of 0.6-1 mM ATP or adenosine induced the outgrowth of numerous processes after 2-3 days that could be observed also in the presence of ACM as previously reported. Culture in ACM plus ATP or adenosine yielded an optimized ramified phenotype. ATP or adenosine, but not ACM alone, also prevented the formation of a flat, amoeboid morphology induced by lipopolysaccharide (LPS); however, at 0.6-1 mM they did not reduce the initial LPS-induced activation of the transcription factor NF-kappaB. By using specific agonists or antagonists the morphological transformations could not be confined to a distinct purinoreceptor subtype, but appeared to be mediated by long-term presence of adenosine in the medium to which phosphorylated purines were rapidly hydrolyzed by microglial cells. Since ACM did not contain sufficient concentrations of ATP or adenosine, purines are not the only ramification-inducing factors present in ACM; however, they are a valuable tool to induce microglial ramification in vitro.

journal_name

J Neuroimmunol

journal_title

Journal of neuroimmunology

authors

Wollmer MA,Lucius R,Wilms H,Held-Feindt J,Sievers J,Mentlein R

doi

10.1016/s0165-5728(01)00257-0

subject

Has Abstract

pub_date

2001-04-02 00:00:00

pages

19-27

issue

1-2

eissn

0165-5728

issn

1872-8421

pii

S0165572801002570

journal_volume

115

pub_type

杂志文章

相关文献

JOURNAL OF NEUROIMMUNOLOGY文献大全
  • Expression of an alpha7 duplicate nicotinic acetylcholine receptor-related protein in human leukocytes.

    abstract：:We examined the potential expression and function of alpha7 nicotinic acetylcholine receptors (nAChRs) in leukocytes. RT-PCR with alpha7 specific primers revealed the presence of the receptor mRNA in leukocytes. Immunoblotting and immunofluorescence experiments demonstrated the expression of a protein that is recogniz...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(02)00057-7

    authors： Villiger Y,Szanto I,Jaconi S,Blanchet C,Buisson B,Krause KH,Bertrand D,Romand JA

    更新日期：2002-05-01 00:00:00

  • Central nervous system infection and immune response in mice inoculated into the lip with herpes simplex virus type 1.

    abstract：:Virus may be recovered from various areas of the central nervous system (CNS) of mice for as long as 11 days after inoculation of herpes simplex virus type 1 (HSV-1) into the lip. The probability of isolation from any particular region of the CNS seems to be a function of the distance of that area from the root-entry ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(82)90062-5

    authors： Kastrukoff L,Hamada T,Schumacher U,Long C,Doherty PC,Koprowski H

    更新日期：1982-06-01 00:00:00

  • Relationships among neurotransmitters, cytokines and cognitive performance for individuals with hepatitis C achieving sustained virologic response: A pilot study.

    abstract：:An important extrahepatic consequence of Hepatitis C is its adverse impact on the central nervous system and cognitive performance. We aimed to determine whether there is a significant relationship between selected neurotransmitters and cytokines and cognitive performance in patients with Chronic Hepatitis C before an...

    journal_title：Journal of neuroimmunology

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1016/j.jneuroim.2019.577022

    authors： Weinstein AA,Estep JM,de Avila L,Curry M,Golabi P,Escheik C,Birerdinc A,Stepanova M,Price JK,Gerber L,Younossi ZM

    更新日期：2019-10-15 00:00:00

  • Rat thymocytes release a factor which inhibits muscarinic ligand binding.

    abstract：:Rat thymocytes incubated in RPMI medium for 120-300 min release a soluble factor of molecular weight below 10,000 Da which inhibits the binding of 3H-labeled muscarinic antagonists in an uncompetitive manner, i.e. it reduces maximal specific binding (Bmax) without changing the affinity of the ligand (KD). This factor ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(88)90119-1

    authors： Maslinski W,Ryzewski J,Bartfai T

    更新日期：1988-03-01 00:00:00

  • Myelin-induced experimental allergic encephalomyelitis in Lewis rats: tumor necrosis factor alpha levels in serum and cerebrospinal fluid immunohistochemical expression in glial cells and macrophages of optic nerve and spinal cord.

    abstract：:Tumor necrosis factor alpha (TNFalpha) activity was measured in serum and cerebrospinal fluid (CSF) of Lewis rats after experimental allergic encephalomyelitis (EAE) induction and during the clinical course of acute disease. TNFalpha bioactivity expression preceded the clinical symptoms and paralleled the severity of ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(95)00151-4

    authors： Villarroya H,Violleau K,Ben Younes-Chennoufi A,Baumann N

    更新日期：1996-01-01 00:00:00

  • Tumour necrosis factor-alpha polymorphism and secretion in myasthenia gravis.

    abstract：:The mechanism behind the association between MHC genes and myasthenia gravis (MG) is not fully understood. In the present study we studied the associations with polymorphisms at HLA-DR3, HLA-B8 and TNF-alpha genes in Swedish patients and healthy individuals. The TNF-alpha-308 allele 2 was associated with female patien...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(98)00253-7

    authors： Huang DR,Pirskanen R,Matell G,Lefvert AK

    更新日期：1999-02-01 00:00:00

  • Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis.

    abstract：:Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of som...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2007.10.004

    authors： D'Aguanno S,Barassi A,Lupisella S,d'eril GM,Del Boccio P,Pieragostino D,Pallotti F,Carelli V,Valentino ML,Liguori R,Avoni P,Bernardini S,Gambi D,Urbani A,Federici G

    更新日期：2008-01-01 00:00:00

  • IFN-gamma up-regulation of IL-12 beta 2 receptor is associated with experimental autoimmune uveitis susceptible B10.A mice, but not in EAU resistant BALB/c mice.

    abstract：:IFN-gamma production is a hallmark of Th1 response, and IFN-gamma has multiple roles in Th1 development, depending on the experimental conditions. In this study, the correlation between IFN-gamma and interleukin 12 receptor beta2 (IL-12R beta 2) expression was investigated in experimental autoimmune uveitis (EAU) susc...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(03)00071-7

    authors： Wu Y,Lin Y,Hou W,Wang Y,Sun B

    更新日期：2003-04-01 00:00:00

  • Protective effect of the SOD/catalase mimetic MnTMPyP on inflammation-mediated dopaminergic neurodegeneration in mesencephalic neuronal-glial cultures.

    abstract：:Reactive oxygen species (ROS) produced by activated microglia are deleterious to neurons. In this study, we studied the effect of Mn(III)tetrakis(1-methyl-4-pyridyl)porphyrin (MnTMPyP), a superoxide dismutase/catalase mimetic, on the lipopolysaccharide (LPS)-induced degeneration of dopaminergic neurons in rat mesencep...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2003.10.040

    authors： Wang T,Liu B,Qin L,Wilson B,Hong JS

    更新日期：2004-02-01 00:00:00

  • Effect of repeated exercise stress on caspase 3, Bcl-2, HSP 70 and CuZn-SOD protein expression in mouse intestinal lymphocytes.

    abstract：:The purpose of this study was to characterize the expression of apoptosis (caspase 3, Bcl-2) and survival (HSP 70, antioxidant CuZn-SOD) proteins in intestinal lymphocytes (IL) of mice after repeated exercise stress. Plasma corticosterone concentration was greater than twofold higher immediately after exercise compare...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2007.04.012

    authors： Hoffman-Goetz L,Spagnuolo PA

    更新日期：2007-07-01 00:00:00

  • Neuronal antigens recognized by cerebrospinal fluid IgM in multiple sclerosis.

    abstract：:Axonal injury is the major cause of disability in patients with multiple sclerosis (MS), but the mechanisms leading to axonal damage are poorly understood. Oligoclonal IgM against lipids predicts an aggressive disease course in MS; however, the antigen that elicits the immune response has not yet been identified. We s...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2012.03.013

    authors： Beltrán E,Hernández A,Lafuente EM,Coret F,Simó-Castelló M,Boscá I,Pérez-Miralles FC,Burgal M,Casanova B

    更新日期：2012-06-15 00:00:00

  • Circulating T-cell subsets in Guillain-Barré syndrome.

    abstract：:We compared the distribution of circulating T-cell subsets within 2 weeks of onset of symptoms in 14 patients with acute Guillain-Barré syndrome (GBS) and 37 normal controls. The levels of OKT4+ (putative helper-inducer) cells was definitely abnormal (decreased) in 3/13 tested. The levels of OKT8+ (putative suppressor...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(85)80050-3

    authors： Lisak RP,Zweiman B,Guerrero F,Moskovitz AR

    更新日期：1985-05-01 00:00:00

  • The change of PD1, PDL1 in experimental autoimmune encephalomyelitis treated by 1,25(OH)2D3.

    abstract：:Experimental autoimmune encephalomyelitis (EAE) is a common animal model that has the same pathology and pathogenesis as multiple sclerosis (MS). Dendritic cells (DCs) exert an important role in central and peripheral tolerance. DCs not only drive T cell priming and differentiation via playing antigen presentation fun...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2019.577079

    authors： Cao Q,Zheng C,Xie Z,Liu L,Zhu J,Jin T

    更新日期：2020-01-15 00:00:00

  • Interleukin-1 activity in lesioned peripheral nerve.

    abstract：:The cytokine interleukin-1 (IL-1) is involved in a wide range of inflammatory and immune responses. As such, IL-1 could play a role in peripheral nerve repair mechanisms. Specifically, by its already established properties as a regulator of nerve growth factor (NGF) synthesis, and as a chemotactant to macrophages. We ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(92)90176-l

    authors： Rotshenker S,Aamar S,Barak V

    更新日期：1992-07-01 00:00:00

  • Metformin ameliorates the development of experimental autoimmune encephalomyelitis by regulating T helper 17 and regulatory T cells in mice.

    abstract：:Immoderate immunoreaction of antigen-specific Th17 and Treg cell dysfunction play critical roles in the pathogenesis of multiple sclerosis. We examined Th17/Treg immune responses and the underlying mechanisms in response to metformin in C57BL/6 mice with experimental autoimmune encephalomyelitis (EAE). Metformin reduc...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2016.01.014

    authors： Sun Y,Tian T,Gao J,Liu X,Hou H,Cao R,Li B,Quan M,Guo L

    更新日期：2016-03-15 00:00:00

  • Role of gut microbiota in the GBR12909 model of mania-like behavior in mice.

    abstract：:Growing evidence suggests a role for brain-gut-microbiota axis in affective disorders including major depression and bipolar disorder (BD). Herein, we aim to explore, by employing germ-free (GF) mice, the effect of the indigenous microbiota in the development of mania-like behavior. Conventional and GF mice were evalu...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2020.577292

    authors： de Miranda AS,Vieira ÉLM,Dos Reis Bastos J,Ferreira RN,Nicoli JR,Teixeira MM,Vieira LQ,Moreira FA,Teixeira AL

    更新日期：2020-06-16 00:00:00

  • Combination treatment of celecoxib and ciprofloxacin attenuates live S. aureus induced oxidative damage and inflammation in murine microglia via regulation of cytokine balance.

    abstract：:Microglial activation is the most common phenomenon in S. aureus induced brain abscesses as well as other common neurodegenerative diseases. The main objective of this study is to reduce the microglial inflammation with effective bacterial elimination. Ciprofloxacin and celecoxib were used in combination to regulate S...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2017.12.006

    authors： Dey R,Sultana S,Bishayi B

    更新日期：2018-03-15 00:00:00

  • Differential effects of interleukin-1beta on neurotoxicity, cytokine induction and glial reaction in specific brain regions.

    abstract：:An appropriate inflammatory response is crucial for the maintenance of tissue homeostasis. The inflammatory responses seen in the brain parenchyma differ from those elicited in the periphery, ventricles and meninges. However, although an inflammatory component has been associated with many CNS diseases, the difference...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2005.07.009

    authors： Depino A,Ferrari C,Pott Godoy MC,Tarelli R,Pitossi FJ

    更新日期：2005-11-01 00:00:00

  • Failure of intravenous immunoglobulin (IVIg) therapy in experimental autoimmune neuritis (EAN) of the Lewis rat.

    abstract：:Experimental autoimmune neuritis (EAN) is an animal model for Guillain-Barré syndrome (GBS). Intravenous immunoglobulins (IVIg) are an effective treatment for GBS, but their mechanism of action is not well understood. Here we tested whether IVIg treatment, a potent modulator of proinflammatory assaults, reduces inflam...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(97)00039-8

    authors： Enders U,Toyka KV,Hartung HP,Gold R

    更新日期：1997-06-01 00:00:00

  • The neurotrophic hepatocyte growth factor induces protolerogenic human dendritic cells.

    abstract：:Hepatocyte growth factor (HGF) limits mouse autoimmune neuroinflammation by promoting the development of tolerogenic dendritic cells (DCs). Given the role played by DCs in the establishment of immunological tolerance, agents that coerce DCs to adopt a protolerogenic function are currently under investigation for multi...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2013.12.004

    authors： Molnarfi N,Benkhoucha M,Juillard C,Bjarnadóttir K,Lalive PH

    更新日期：2014-02-15 00:00:00

  • Dopaminergic receptors and adrenoceptors in circulating lymphocytes as putative biomarkers for the early onset and progression of multiple sclerosis.

    abstract：:Clinically isolated syndrome (CIS) is a first, usually recovering, episode of neurological disturbance(s) suggestive of multiple sclerosis (MS). CIS subjects might benefit from early disease-modifying drugs, provided that those at high risk of developing MS can be identified. Gene expression for dopaminergic receptors...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2016.07.008

    authors： Cosentino M,Zaffaroni M,Legnaro M,Bombelli R,Schembri L,Baroncini D,Bianchi A,Clerici R,Guidotti M,Banfi P,Bono G,Marino F

    更新日期：2016-09-15 00:00:00

  • Continuous in vivo treatment with catecholamines suppresses in vitro reactivity of rat peripheral blood T-lymphocytes via alpha-mediated mechanisms.

    abstract：:A 20 h continuous treatment of rats with catecholamines, using subcutaneously implantable retard tablets, had either no (adrenaline, isoproterenol, midodrine) or a slight (noradrenaline) suppressive effect on the in vitro responsiveness of peripheral blood T-lymphocytes. A marked suppression of the mitogen response en...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(92)90154-d

    authors： Felsner P,Hofer D,Rinner I,Mangge H,Gruber M,Korsatko W,Schauenstein K

    更新日期：1992-03-01 00:00:00

  • Glial transforming growth factor (TGF)-beta isotypes in multiple sclerosis: differential glial expression of TGF-beta 1, 2 and 3 isotypes in multiple sclerosis.

    abstract：:We studied glial transforming growth factor (TGF)-beta isotype expression in 14 cases of multiple sclerosis. Acute active lesions exhibited selective TGF-beta 2 immunoreactivity of lesion encircling ramified microglia. In contrast, astrocytes within chronic active white matter lesions expressed all three isotypes. Chr...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(96)00135-x

    authors： Peress NS,Perillo E,Seidman RJ

    更新日期：1996-12-01 00:00:00

  • Expression of the urokinase plasminogen activator and its receptor in HIV-1-associated central nervous system disease.

    abstract：:The urokinase plasminogen activator (uPA) and its receptor (uPAR) play important physiological functions in extracellular proteolysis, as well as cell adhesion and migration. Through dysregulation of these functions, the uPA/uPAR system might be involved in the pathogenesis of AIDS dementia complex (ADC), and, in fact...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2004.08.038

    authors： Sidenius N,Nebuloni M,Sala S,Zerbi P,Price RW,Gisslen M,Hagberg L,Vago L,Lazzarin A,Blasi F,Cinque P

    更新日期：2004-12-01 00:00:00

  • Preferential association of V lambda x light chains with gamma 2a heavy chains in naturally occurring human myelin basic protein reactive antibodies.

    abstract：:Active immunization with myelin basic protein (MBP) induces experimental allergic encephalomyelitis (EAE) in a variety of animal species, including rats and mice. We have previously described the ability of the newly described mouse lambda (lambda) variable (V) region V lambda x, to confer MBP reactivity to an Ab. In ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(96)00072-0

    authors： Galin FS,Zhou SR,Whitaker JN,Blalock JE

    更新日期：1996-10-01 00:00:00

  • False-positive acetylcholine receptor antibody results in patients without myasthenia gravis.

    abstract：:Acetylcholine receptor antibodies are very specific for myasthenia. During a large prospective cohort study of myasthenia, we encountered five patients, positive for acetylcholine receptor (AChR) antibodies by radioimmunoprecipitation assay (RIA), whose clinical course revealed diagnoses other than myasthenia. Two pat...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2019.04.001

    authors： Maddison P,Sadalage G,Ambrose PA,Jacob S,Vincent A

    更新日期：2019-07-15 00:00:00

  • Parental genes do not codominantly confer susceptibility to experimental autoimmune encephalomyelitis in F1 rats.

    abstract：:Lewis (LEW) and DA rats are highly susceptible to experimental autoimmune encephalomyelitis (EAE) induced with guinea pig myelin basic protein (MBP), but respond to different epitopes. The dominant epitope for LEW rats is MBP73-86, and disease is mediated primarily by Vbeta8.2 Th1 cells. DA rats lack conventional Vbet...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(01)00244-2

    authors： Lenz DC,Wolf NA,Swanborg RH

    更新日期：2001-03-01 00:00:00

  • MicroRNAs associated with the pathogenesis of multiple sclerosis.

    abstract：:Multiple sclerosis (MS) is not only an autoimmune disease in which autoreactive immune cells against myelin damage axons and nerves in the central nervous system, but also a neurodegenerative disease, in which progressive loss of structure and function of neurons occurs. The mechanisms of MS pathogenesis have not been...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jneuroim.2016.04.014

    authors： Huang Q,Xiao B,Ma X,Qu M,Li Y,Nagarkatti P,Nagarkatti M,Zhou J

    更新日期：2016-06-15 00:00:00

  • Immunoblot and immunohistochemical comparison of murine monoclonal antibodies specific for the rat D1a and D1b dopamine receptor subtypes.

    abstract：:The two D1-like dopamine receptor subtypes, D1a and D1b, are structurally similar and pharmacologically indistinguishable using currently available ligands. To differentiate between the D1-like dopamine receptor subtypes, murine monoclonal antibodies to the rat Dla and the rat D1b dopamine receptor have been prepared....

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(99)00142-3

    authors： Luedtke RR,Griffin SA,Conroy SS,Jin X,Pinto A,Sesack SR

    更新日期：1999-11-15 00:00:00

  • Unique HLA haplotype associations in IgG4 anti-neurofascin 155 antibody-positive chronic inflammatory demyelinating polyneuropathy.

    abstract：:To clarify the immunogenetic background of patients with immunoglobulin G (IgG)4 anti-neurofascin 155 (NF155) antibody-positive chronic inflammatory demyelinating polyneuropathy (CIDP), we genotyped the extended human leukocyte antigen (HLA) haplotypes in 22 Japanese patients with this disorder and compared them with ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2019.577139

    authors： Ogata H,Isobe N,Zhang X,Yamasaki R,Fujii T,Machida A,Morimoto N,Kaida K,Masuda T,Ando Y,Kuwahara M,Kusunoki S,Nakamura Y,Matsushita T,Kira JI

    更新日期：2020-02-15 00:00:00