Abstract：
:Immunomagnetic systems have been used for positive selection of a cell fraction from a mixture using appropriate surface markers with satisfactory results, as haematopoietic CD34+ cells. This work reports on the development of poly(ethylene glycol)-grafted (PEG) immunoliposomes loaded with citrate-magnetite stabilized particles as the separation vehicles for immunomagnetic separations. The magnetic ferrofluid was encapsulated into PEG-liposomes by the DRV methodology. The magnetoliposomes had a liposomal size of approximately 450 nm and a Fe/lipid molar ratio of 1.52+/-0.26, and were retained in the magnetic field created by the MiniMACS system. Anti-CD34 immunomagnetoliposomes with 100 mAb/vesicle were prepared by coupling the My10 mAb and bound specifically for CD34+ KG-1a cells in culture and in mixtures with CD34-cells (CHO or Jurkat). The magnetic cell sorting was carried out in cell mixtures KG-1a/CHO or KG-1a/Jurkat with different initial% of CD34+ Kg-1a cells. For 10(6) positive cells and 100 microM of immunomagnetoliposomes, the capture efficiency was > 85% and independent of the starting percentage of CD34+ cells. The decrease of the final purity, when the starting percentage of CD34+ cells decreases and, dependent of the CD34- cell line used, point to the degree of non-specific cell binding of My10-immunomagnetoliposomes as being crucial, among of the methodological aspects as the number of starting CD34+ cells. The CD34+ cells isolated retained the viability with an estimated recovery of 45-50%.
journal_name
J Microencapsuljournal_title
Journal of microencapsulationauthors
Domingo JC,Mercadal M,Petriz J,De Madariaga MAdoi
10.1080/026520401750038593subject
Has Abstractpub_date
2001-01-01 00:00:00pages
41-54issue
1eissn
0265-2048issn
1464-5246journal_volume
18pub_type
杂志文章abstract：:Optimum conditions for the preparation of non-magnetic and magnetic microspheres of albumin-globulin mix (alglomix) containing mefenamic acid have been standardized. The effect of various parameters has been investigated with regard to the appearance, yield, drug content and encapsulation efficiency. The physicochemic...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652049109021856
更新日期：1991-01-01 00:00:00
abstract：:In recent years, wormwood has been successfully applied in various fields, such as flavouring agent, plant dye, insect repellent and medical product. Wormwood oil encapsulated microcapsules with continuous release behaviours would be further applied in the health care textiles. Here, wormwood oil encapsulated microcap...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652048.2020.1749320
更新日期：2020-06-01 00:00:00
abstract：:Silica gel microspheres are ideal materials for bioencapsulation due to their mechanical properties, biocompatibility, and stability. Encapsulated cells are isolated from the environment and protected from predators, changes in pH, and osmotic stress. However methods for the production of silica gel microspheres suita...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652048.2016.1202346
更新日期：2016-08-01 00:00:00
abstract：:Graft copolymer polyethylenimine-graft-poly(N-vinylpyrrolidone) (PEI-g-PVP) was prepared by coupling mono carboxyl-terminated PVP (PVP-COOH) with PEI using N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) as coupling agents. In aqueous medium, PVP-g-PEI can self-assemble into stable polyion complex m...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048.2010.534823
更新日期：2011-01-01 00:00:00
abstract：:A new material for the microencapsulation of biological systems was discovered and characterized with regards to the effects of reaction conditions on product yield. By using poly(cyanoacrylate ester), membrane microcapsules were produced with sufficient strength and porosity to be effective in a process environment f...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652040110055225
更新日期：2002-11-01 00:00:00
abstract：:The body distribution of surfactant-coated and non-coated poly(methyl methacrylate) nanoparticles with a size of 131 +/- 30 nm after intravenous injection into rats was investigated. The coating materials were poloxamine 904, poloxamine 908, poloxamine 1508, poloxamer 338, and Brij 35. These materials were preselected...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652049209021219
更新日期：1992-01-01 00:00:00
abstract：:Polyisohexylcyanoacrylate nanocapsules containing cyclosporin were prepared by mixing in a 1:2 ratio an oil/ethanol solution of monomer and drug with an aqueous phase. Drug nanoencapsulation rate was controlled by its partition coefficient between the inner (organic) and outer (aqueous) phases. Thus highest encapsulat...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652049109021233
更新日期：1992-04-01 00:00:00
abstract：:Bacampicillin hydrochloride has been microencapsulated to mask its very bitter taste. The objective of the study was to compare the in vitro release and bioavailability of bacampicillin hydrochloride from microcapsules coated with two principally different polymers: a water-insoluble polymer, ethylcellulose, and an ac...
journal_title：Journal of microencapsulation
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.3109/02652048509031556
更新日期：1985-04-01 00:00:00
abstract：:The size dependent and size independent contributions to the mechanical properties of poly(ethyleneglycol) microspheres with encapsulated air, made for medical ultrasound imaging, have been studied. A relation between the size of microspheres in a reference group and their acoustic attenuation efficacy was derived by ...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/026520400288292
更新日期：2000-05-01 00:00:00
abstract：:We studied a model system of controlled drug release using beta-carotene and κ-carrageenan/NaCMC hydrogel as a drug and a device, respectively. Different concentrations of genipin were added to crosslink the beta-carotene loaded beads by using the dripping method. Results have shown that the cross-linked beads possess...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048.2011.651501
更新日期：2012-01-01 00:00:00
abstract：:Polyurethane modified with polylactic acid microcapsules were fabricated for controlled release of chlorpyrifos (one of the high usage solid phosphorous insecticides) via interfacial polymerisation with diphenylmethane diisocyanate, polyether triol, 1,4-butanediol and polylactic acid as modifier. The structure, morpho...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652048.2019.1599075
更新日期：2019-01-01 00:00:00
abstract：:This study demonstrates the feasibility of using a single-capillary electrospray (ES) system to generate novel alpha-lipoic acid encapsulated poly(ethylene oxide)-chitosan (ALA/PEO/CS) particles with a monodispersed diameter. Scanning electron microscopic images (SEM) and dynamic light scattering (DLS) results indicat...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048.2013.863395
更新日期：2014-01-01 00:00:00
abstract：:The sorption of 5-fluorouracil from aqueous solutions onto poly(methyl methacrylate) was investigated. Linear sorption isotherms were obtained and no crystallinity of the nanoparticles after sorption was detectable by X-ray analysis indicating a partitioning of the drug into the polymer particles-forming a solid solut...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048609031560
更新日期：1986-04-01 00:00:00
abstract：:The use of polymeric carriers in formulations of therapeutic drug delivery systems has gained widespread application, due to their advantage of being biodegradable and biocompatible. Among the microparticulate systems, microspheres have a special importance since it is possible to target drugs and provide controlled r...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/026520400288382
更新日期：2000-03-01 00:00:00
abstract：:Conditions of microencapsulation by in situ polycondensation, using melamine-formaldehyde as wall material, are influenced by the chemical nature of the core to encapsulate. In our study concerning the encapsulation of a photopolymerizable phase containing an electrically charged compound, it was necessary to modify t...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652049309031522
更新日期：1993-07-01 00:00:00
abstract：:In this work we have examined the ability of various lipopolyplexes to deliver genes into liver cancer cells. We evaluated different parameters such as the protocol of preparation, the lipid/DNA molar ratio, and the molecular weight and type of PEI, to optimize the formulation to achieve high transfection activity. Ou...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048.2010.501396
更新日期：2010-01-01 00:00:00
abstract：:The bioavailability of marketed nitrofurantoin capsules was compared to capsules filled with Gantrez-gelatin nitrofurantoin microcapsules of a core:coat ratio of 1:2. The collective results obtained from the analysis of urine samples of five volunteers indicated that nitrofurantoin microcapsules provided a prolonged r...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048809036727
更新日期：1988-10-01 00:00:00
abstract：:The factors influencing the encapsulation of azathioprine (AZA) into liposomes were investigated to find out the conditions for its optimal entrapment. Similar studies for comparison were also carried out on 6-mercaptopurine (6-MP), of which AZA is a prodrug. AZA and also 6-MP show higher encapsulation efficiencies in...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652049809006875
更新日期：1998-07-01 00:00:00
abstract：:For the preparation of microcapsules using the W/O/W (water in oil in water) emulsion system, it is essential to control various factors such as the dispersed state of the organic phase in the W/O/W emulsion, the difference in the solute concentration between the inner and outer aqueous phases and the volume fraction ...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/0265204031000093096
更新日期：2003-07-01 00:00:00
abstract：:Rosin was esterified with pentaerythritol and intermediate reaction products with different acid values were isolated. Microcapsules containing sulphadiazine were prepared by a solvent evaporation technique using these esters as wall materials. The microcapsules were evaluated for their size, drug content, and release...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652049409040434
更新日期：1994-01-01 00:00:00
abstract：:The degradation of polybutylcyanoacrylate (PBC) and polyhexylcyanoacrylate (PHC) nanoparticles, together with their association with and toxicity towards isolated hepatocytes, were determined. Nanoparticles were not taken up by rat hepatocytes at a significant level. The LD50S of PBC and PHC nanoparticles towards hepa...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048409049364
更新日期：1984-07-01 00:00:00
abstract：:A kind of electronic ink, which is supported on indium-tin-oxide (ITO) glass before polymerization, is prepared using urea and formaldehyde resin as wall materials, in which TiO(2) modified with PMMA are dispersed in tetrachloroethylene (TCE) using a mixture of oil blue dyes and charge control additive (span80). Rotar...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652040600788197
更新日期：2006-11-01 00:00:00
abstract：:Ibuprofen-loaded polystyrene microparticles were prepared by the emulsion-solvent evaporation process from an aqueous system. The effects of different parameters on the drug content and on the release of the drug from the microparticles were investigated. The drug content, in all the formulations, was less than the th...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/026520400288553
更新日期：2000-01-01 00:00:00
abstract：:For slow release of tea tree oil (TTO), TTO were encapsulated by urea-formaldehyde (UF) resin via in situ polymerisation. The effects of curing time and drying condition on particle size, and TTO loading of the TTO/UF microcapsules were investigated. The results indicated that TTO/UF resin microcapsules with curing ti...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652048.2020.1735548
更新日期：2020-06-01 00:00:00
abstract：:This paper prepared novel biodegradable and pH-sensitive microgels based on Poly(epsilon-caprolactone)-Pluronic-Poly(epsilon-caprolactone)-dimethacrylate (PCFC-DMA), Poly(ethylene glycol) dimethacrylate (PEG-DMA) and methylacrylic acid (MAA) cross-linked with N,N'-methylenebisacrylamide (BIS), initiated by NaHSO(3), K...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652040802610827
更新日期：2009-11-01 00:00:00
abstract：:Anal fissure is common and painful disease of anorectum. In this study, microparticles containing nifedipine and lidocaine HCl were prepared by spray drying and applied to bio-degradable and bio-stable tampons. Characterization of microparticles was determined by visual analyses, mass yield, particle size measurement,...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652048.2017.1393113
更新日期：2017-12-01 00:00:00
abstract：:Ionotropic gelation was used to entrap sulindac into calcium alginate beads as a potential drug carrier for the oral delivery of this anti-inflammatory drug. Beads were investigated in vitro for a possible sustained drug release and their use in vivo as a gastroprotective system for sulindac. Process parameters such a...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/02652040701298153
更新日期：2007-06-01 00:00:00
abstract：:The aim of the present study is to construct a type of polyion complex micelles made of PF127-PEI copolymer and cholic acid (CA) and to evaluate the potential of this type of micelles as a targeted drug delivery system for paclitaxel (PTX). To further improve the targeting capability of micelles, folate was also incor...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652048.2014.940010
更新日期：2014-01-01 00:00:00
abstract：:In this study, ethylcellulose and acrylate-methacrylate copolymer (Eudragit RL 100, Eudragit RS 100) membranes were prepared by using appropriate types and amounts of plasticizers. Thirty percent and 20% of triacetin based on polymer weight were found to be appropriate plasticizers for ethylcellulose membranes and acr...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.3109/02652049609026048
更新日期：1996-11-01 00:00:00
abstract：:This work investigated the means for the efficient encapsulation of muramyl dipeptide (MDP) in poly(epsilon-caprolactone) (PCL) microparticles (MP) by a solvent evaporation method in order to optimize the effect of the adjuvant for oral immunization. Therefore, the influence of MDP concentration in the inner aqueous p...
journal_title：Journal of microencapsulation
pub_type： 杂志文章
doi：10.1080/026520499288799
更新日期：1999-09-01 00:00:00
