Chemical forms of selenium present in rat and ram spermatozoa.

Abstract:

:In vivo and in vitro studies were conducted to investigate the chemical forms by ion-exchange chromatography of selenium (Se) present in rat and ovine spermatozoa. After injection with 75Se-selenite, the form of 75Se in rat sperm was selenocysteine, but selenocysteine and selenomethionine (SeMet) were present in ovine sperm. Presumably, synthesis of SeMet by rumen microbes are responsible for its presence in ovine sperm. In vitro incubation of ram sperm with selenocysteine or SeMet produced no changes, but incubation with selenite produced a compound that eluted one fraction before SeMet from the ion-exchange column. After treatment of this fraction with mercaptoethanol, it eluted in a later fraction upon rechromatography, suggesting it to be selenodicysteine. This compound is apparently formed because of high levels of cysteine in semen. Cysteine, reduced glutathione, and oxidized glutathione were also found in semen. The significance of the results is discussed.

journal_name

Biol Trace Elem Res

authors

Alabi NS,Beilstein MA,Whanger PD

doi

10.1385/bter:76:2:161

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

161-73

issue

2

eissn

0163-4984

issn

1559-0720

journal_volume

76

pub_type

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