Abstract:
:The aim of this study was to examine the effects of cadmium in concentrations relevant to those detected in human serum on cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) expression at mRNA, protein, and enzyme activity levels in THP-1 macrophages. Macrophages were incubated with various cadmium chloride (CdCl2) solutions for 48 h at final concentrations of 5 nM, 20 nM, 200 nM, and 2 μM CdCl2. The mRNA expression and protein levels of COXs were analyzed with RT-PCR and Western blotting, respectively. Prostaglandin E2 (PGE2) and stable metabolite of thromboxane B2 (TXB2) concentrations in culture media were determined using ELISA method. Our study demonstrates that cadmium at the highest tested concentrations modulates COX-1 and COX-2 at mRNA level in THP-1 macrophages; however, the lower tested cadmium concentrations appear to inhibit COX-1 protein expression. PGE2 and TXB2 production is not altered by all tested Cd concentrations; however, the significant stimulation of PGE2 and TXB2 production is observed when macrophages are exposed to both cadmium and COX-2 selective inhibitor, NS-398. The stimulatory effect of cadmium on COXs at mRNA level is not reflected at protein and enzymatic activity levels, suggesting the existence of some posttranscriptional, translational, and posttranslational events that result in silencing of those genes' expression.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Olszowski T,Gutowska I,Baranowska-Bosiacka I,Piotrowska K,Korbecki J,Kurzawski M,Chlubek Ddoi
10.1007/s12011-015-0234-6subject
Has Abstractpub_date
2015-06-01 00:00:00pages
135-44issue
2eissn
0163-4984issn
1559-0720journal_volume
165pub_type
杂志文章abstract::The characteristics of aluminum and chromium binding to apo-transferrin (apo-tf) have been investigated and compared. Both metal ions were taken up by human transferrin forming complexes with the maximum absorbances at 405 nm for chromium-transferrin (cr-tf) and 240 nm for aluminum-transferrin (Al-tf). In the presence...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784585
更新日期:1992-01-01 00:00:00
abstract::The study was conducted to evaluate the effects of chromium-loaded chitosan nanoparticles (Cr-CNP) on glucose transporter 4 (GLUT4), relevant messenger RNA (mRNA), and proteins involved in phosphatidylinositol 3-kinase (PI3K), Akt2-kinase, and AMP-activated protein kinase (AMPK) of skeletal muscles in finishing pigs. ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-016-0890-1
更新日期:2017-07-01 00:00:00
abstract::The effect of maternal dietary selenium (Se) and gestation on the concentrations of Se and zinc (Zn) in the porcine fetus were determined. Mature gilts were randomly assigned to treatments of either adequate (0.39 ppm Se) or low (0.05 ppm Se) dietary Se. Gilts were bred and fetuses were collected throughout gestation....
journal_title:Biological trace element research
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
abstract:CONTEXT:Complementary alternative medicine therapies based on the use of cesium chloride preparations for the treatment of cancer and radiation poisoning, have generated therapeutic interest; but oral or intravenous administration of cesium chloride (CsCl) to cancer patients as an alternative mode of cancer therapy hav...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:94:2:97
更新日期:2003-08-01 00:00:00
abstract::The causes of night blindness in children are multifactorial, and particular consideration has been given to childhood nutritional deficiency, which is the most common problem found in underdeveloped countries. Such deficiency can result in physiological and pathological processes that in turn influence hair compositi...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-010-8789-8
更新日期:2011-09-01 00:00:00
abstract::Although coenzyme Q10 (CoQ10) is a component of the oxidative phosphorylation process in mitochondria that converts the energy in carbohydrates and fatty acids into ATP to drive cellular machinery and synthesis, its effect in type I diabetes is not clear. We have studied the effect of 4 wk of treatment with CoQ10 (10 ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:109:1:025
更新日期:2006-01-01 00:00:00
abstract::Aspirin is widely used as an antiinflammatory drug especially in children with rheumatic fever arthritis. The diminishing effects of aspirin on antioxidant enzymes and hepato-renal systems at high doses are well-known. It is now evident that the damage at antioxidant system worsens the clinical picture of the disease ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-008-8115-x
更新日期:2008-07-01 00:00:00
abstract::This experiment was conducted to evaluate the effects of zinc (ZnSO4.H2O) and vitamin A (retinol) supplementation on performance, carcass characteristics, and serum concentrations of glucose, cholesterol, total protein, and malondialdehyde (MDA) as an indicator of lipid peroxidation in broiler chickens (Ross) reared a...
journal_title:Biological trace element research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1385/BTER:94:3:225
更新日期:2003-09-01 00:00:00
abstract::Iron-deficiency anemia (IDA) is the most common nutritional deficiency in childhood throughout the world. Although it has been shown that IRA is associated with elevated plasma copper and depleted zinc levels in children, there are conflicting results on the effect of iron supplementation on the absorption of these el...
journal_title:Biological trace element research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1385/BTER:94:1:79
更新日期:2003-07-01 00:00:00
abstract::The kidney has been regarded as a critical organ of toxicity induced by acute exposure to hexavalent chromium [Cr(VI)] compounds. Reactive intermediates and free radicals generated during reduction process might be responsible for Cr(VI) toxicity. In this study, the effects of pretreatment or posttreatment of taurine ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:102:1-3:209
更新日期:2004-01-01 00:00:00
abstract::Chronic alcoholics frequently show associated malnutrition. Both ethanol and malnutrition exert profound changes on zinc and copper metabolism. In this study, we found higher hair zinc and copper values in 43 male alcoholics than in 39 controls. Hair copper was significantly related to the amount of ethanol consumed, ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/bter:85:3:269
更新日期:2002-03-01 00:00:00
abstract::Aluminum (Al) exposure inhibits bone formation. Osteoblastic proliferation promotes bone formation. Therefore, we inferred that Al may inhibit bone formation by the inhibition of osteoblastic proliferation. However, the effects and molecular mechanisms of Al on osteoblastic proliferation are still under investigation....
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-016-0880-3
更新日期:2017-06-01 00:00:00
abstract::Prescription food for special medical purposes (FSMPs) and modified milk products (MMPs) for babies in the infancy period available in pharmacies are increasingly frequently used for newborns and infants-especially in cases where allergies and food-borne diseases are diagnosed. However, there is a lack of scientific s...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-019-01667-w
更新日期:2019-12-01 00:00:00
abstract::In vitro distribution of aluminium between plasma and erythrocytes has been studied in the presence of variable amounts of sodium L-glutamate. With a red blood cell suspension in isotonic sodium chloride, aluminium remains confined in erythrocytes even when the sodium L-glutamate concentration increases in the medium....
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02990262
更新日期:1990-04-01 00:00:00
abstract::Microcalorimetry was employed to investigate the action of Li(I) to aquatic ecosystem from the point view of bioenergetics. Tetrahymena thermophila BF5 was chosen as the model organism. The power-time curves of T. thermophila BF5 growth metabolism in the absence and presence of Li(I) were obtained. The corresponding t...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-007-0040-x
更新日期:2007-10-01 00:00:00
abstract::Quercetin, a plant-derived flavonoid, is frequently used as an antioxidant for efficient anti-oxidative capacity. However, whether quercetin has protective effects on goat sperm and preimplantation embryos against Cd2+-induced oxidative injury is still unclear. So, we researched the influence of quercetin on goat sper...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1255-8
更新日期:2018-10-01 00:00:00
abstract::A simple, quick procedure has been developed for preparation of human lenses for total chromium (Cr) analysis by electrothermal atomic absorption spectrometry (EAAS). This procedure involves wet ashing in a mini-autoclave with HNO3 and H2SO4. Recovery was 101.6%. This procedure is simple to carry out, does not generat...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784598
更新日期:1992-01-01 00:00:00
abstract::Long-term copper application in vineyards and copper mining activities cause heavy metal pollution sites. Such sites need remediation to protect soil and water quality. Bioremediation of contaminated areas through bioleaching can help to remove copper ions from the contaminated soils. Thus, the aim of this work was to...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-011-9213-8
更新日期:2012-04-01 00:00:00
abstract::Selenium (Se) is an essential trace element for both humans and animals. Se deficiency leads to myocardial injury, reproductive disorder, increased exudation, inflammatory injury, and other diseases. The present study analyzed the relationships of Se deficiency, inflammation, and smooth muscle contraction in the small...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02245-1
更新日期:2020-06-15 00:00:00
abstract::Cadmium concentrations were determined in the hair of 226 school children in an industrial and in a rural area of Tarragona Province (NE Spain). The influence of sex, age, hair color, smoking habits of the household members, and parents' occupation on the children's hair cadmium levels was also evaluated. Children liv...
journal_title:Biological trace element research
pub_type: 杂志文章
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更新日期:1991-02-01 00:00:00
abstract::Osteoporosis is a bone disease that leads to an increased risk of fracture. Oxidative stress may play a major role in the development of osteoporosis in part by inhibiting osteoblastic differentiation of bone marrow stromal cells (MSCs). Some evidence suggested that antioxidant selenium could prevent osteoporosis, but...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-012-9488-4
更新日期:2012-12-01 00:00:00
abstract::Colon cancer in men and breast cancer in women are regarded as major health burdens, accounting for majority of cancer diagnoses globally. Doxorubicin (DOX) resistance in breast and colon cancers represents the main reason of unsuccessful therapy. The rationale of this study is to explore whether selenium nanoparticle...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-019-01730-6
更新日期:2020-02-01 00:00:00
abstract::In subjects with impaired insulin action, alterations of the serum sodium and potassium concentrations have been reported. The resulting cationic imbalance, along with the osmotic effect of the elevated sugar levels, could influence the course of diabetes mellitus management. Therefore, this study was conducted to com...
journal_title:Biological trace element research
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12011-011-9144-4
更新日期:2011-12-01 00:00:00
abstract::Zinc has been reported to enhance the response to interferon (IFN) or PEG-IFN plus ribavirin therapy, improve liver function, and ameliorate hematologic side effects in patients with chronic hepatitis C. However, the role of zinc supplementation during IFN therapy in chronic hepatitis B infection (CHB) remains unclear...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-011-8971-7
更新日期:2011-12-01 00:00:00
abstract::AFB1 is a mycotoxin which exerts their cytotoxicity through increasing oxidative damage in target organ. Kidney is one of target organs vulnerable to damage caused by AFB1. In this study, Madin-Darby canine kidney (MDCK) cells were used to evaluate the AFB1-induced cell damage by the MTT assay. The results revealed th...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-9887-9
更新日期:2014-03-01 00:00:00
abstract::The aim of this work is to verify whether there are statistically significant correlations between the concentrations of lead in blood, urine, and hair in children. The sample collected in 2007 consists of 163 children of both sexes from 11-14-year-olds, living in three municipalities of Sardinia (Italy). Inductively ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-011-8988-y
更新日期:2011-12-01 00:00:00
abstract::In the current study, 48 male rats were classified into four groups (12 rats/group): 1-control group received 1 ml distilled water, 2-origanum oil group treated daily with oral dose of origanum oil (5 mg/kg) for 30 and 60 days, 3-mercuric chloride group treated daily with oral dose of mercuric chloride (4 mg/kg) for 3...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-017-1067-2
更新日期:2018-03-01 00:00:00
abstract::The phosphorus NMR profile of normal and vanadium-treated chick muscle was obtained in vivo. The data show that the differentiation of breast and thigh muscles in terms of pH, lipid related metabolites, and bioenergetic parameters can be readily followed. Although the vanadium-treated chicks showed substantial retarda...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02990427
更新日期:1991-11-01 00:00:00
abstract::Exposure of people and animals to arsenic (As) is a global public health concern because As is widely distributed and associated with numerous adverse effects. As is a poisonous metalloid and arsenic trioxide (As2O3) is a form of As. Thus far, there have been very few reports on the inflammatory factor alterations of ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-015-0305-8
更新日期:2015-10-01 00:00:00
abstract::A dose-response experiment was conducted to find the sensitive and consistent biomarker for the estimation of dietary manganese (Mn) requirement and establish the optimal Mn level for broilers fed a practical corn-soybean meal diet from 1 to 21 days of age post-hatching. A total of 480 1-day-old Arbor Acres male chick...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-010-8931-7
更新日期:2011-11-01 00:00:00