Abstract:
:Previous studies showed that different HLA-A and -B antigens are differentially expressed in cells. Their relative quantities are genetically predetermined and inherited according to Mendelian laws. To investigate mechanisms responsible for this differential expression, a correlation study between the relative quantities of different HLA-A and -B proteins and their mRNA levels in eight different HLA-phenotyped lymphoblastoid cell lines (LCLs) were performed. The results show proportional correlation in all the studied cell lines except those that are positive for HLA-A24. Study of the turnover of HLA antigens reveals that different HLA-A and -B antigens are proportionally degraded. Measurement of the relative quantities of HLA-A and -B mRNAs in six LCLs before and after treatment with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), an inhibitor of RNA polymerase II, demonstrates that HLA-A and -B mRNAs are proportionally degraded except slight differences in two LCLs. Measurement of the relative quantities of different HLA-A and -B pre-mRNAs in nuclei shows that they are not proportional to the relative quantities of their respective mature mRNAs in cytoplasm in four of six LCLs. These results indicate that combinations of different regulatory steps which include gene transcription, pre-mRNA splicing and mRNA degradation are involved in the genetically predetermined quantitative differential expression of HLA-A and -B antigens. Transcription of HLA genes and splicing of HLA pre-mRNAs appear to be the dominant regulatory steps.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Liu K,Kao KJdoi
10.1016/s0198-8859(00)00142-7subject
Has Abstractpub_date
2000-08-01 00:00:00pages
799-807issue
8eissn
0198-8859issn
1879-1166pii
S0198-8859(00)00142-7journal_volume
61pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract::A flow cytometric method of simultaneously screening both HLA Class I and Class II panel reactive antibodies (PRA) was developed using a pool of 30 different Class I and 30 different Class II microbeads coated with purified HLA antigens. The antibodies in the serum that react specifically to the coated HLA antigens ar...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(98)00020-2
更新日期:1998-05-01 00:00:00
abstract::The loss of melanocytes in vitiligo is mainly attributed to defective autoimmune mechanisms and lately autoinflammatory mediators have become more emphasized. Among these, a number of class II cytokines and their receptors have displayed altered expression patterns in vitiligo. Thus, we selected 30 SNPs from the regio...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2015.09.050
更新日期:2016-05-01 00:00:00
abstract::The relevance of pre-existing HLA-antibodies (HLA-Ab) in liver transplantation (LTx) is controversial. While livers are allocated without HLA match or preoperative crossmatch testing, several studies point to an impact of donor specific antibodies (DSA) for acute rejection, bile duct complications or even graft loss. ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2018.04.009
更新日期:2018-07-01 00:00:00
abstract::Using a large battery of Bw16, w38, and w39 antisera, a new variant of Bw16 has been identified in four unrelated Mexican-American families. The serologic pattern obtained is distinct from that for Bw38, Bw39, and 8W57 antigens. Absorption studies confirm the existence of this new Bw16 subtype which we term ST-16. ST-...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(82)90050-7
更新日期:1982-02-01 00:00:00
abstract::Dendritic cells (DCs) exposed to tumor antigens followed by treatment with T(h)1-polarizing differentiation signals have paved the way for the development of DC-based cancer vaccines. Critical parameters for assessment of the optimal functional state of DCs and prediction of the vaccine potency of activated DCs have i...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.10.001
更新日期:2010-01-01 00:00:00
abstract::Mucin 1 is a cell-membrane associated mucin, expressed on epithelial and immune cells that helps protect against pathogenic infections. In humans, MUC1 is highly polymorphic, predominantly due to the presence of a variable number tandem repeat (VNTR) region in the extracellular domain that results in MUC1 molecules of...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2019.06.001
更新日期:2019-10-01 00:00:00
abstract::The HLA-B5,B35 cross-reacting group is a large and serologically complex antigen family which includes the World Health Organization-recognized specificities HLA-B5,B51,Bw52,B35,Bw53,B18,Bw70,Bw71, and Bw72. In addition, several variants of antigens in this cross-reacting group have been described in the past but have...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(89)90035-9
更新日期:1989-11-01 00:00:00
abstract::In previous studies, major histocompatibility complex (MHC) class II DP, DQ, and DR families of genes were characterized in different primate species mostly on the basis of their second exon sequences. Resemblances were found between Old World monkey (OWM) and New World monkey (NWM) genes and were interpreted as being...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(00)00233-0
更新日期:2001-01-01 00:00:00
abstract::Allogeneic hematopoietic stem cell transplantation (alloHCT) is the definitive therapy for numerous otherwise incurable hematologic malignancies and non-malignant diseases. The genetic disparity between donor and recipient both underpins therapeutic effects and confers donor immune system-mediated damage in the recipi...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2019.03.003
更新日期:2019-06-01 00:00:00
abstract::We compared the immunohistochemical reaction patterns of HLA-G-specific antibodies 87G, 4H84, G233, 16G1, and BFL.1 on human placentas under three different preparative conditions and on cryosections of other human tissues. Human and murine cell lines, either naturally expressing or transfected with HLA-G, were analyz...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(00)00207-x
更新日期:2000-11-01 00:00:00
abstract::Five techniques, the direct and the antiglobulin enhanced cytotoxicity assays, indirect immunofluorescence, the enzyme-linked immunosorbent assay, and the radioimmunoassay, were evaluated in a workshop to determine their utility in studies of the interactions of monoclonal antibodies with HLA antigens expressed on lym...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(82)90016-7
更新日期:1982-04-01 00:00:00
abstract::To simplify PCR-SSO HLA-DRB generic typing, we labeled eight of 15 oligonucleotide probes with DIG and the others with biotin, and hybridized each dot blot with both a biotin-labeled probe and a DIG-labeled probe simultaneously. We chose oligonucleotide pairs which require the same hybridization and stringent washing ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(94)90097-3
更新日期:1994-01-01 00:00:00
abstract::The human leukocyte antigen (HLA) genes are highly variable and are known to play an important role in disease outcomes, including infectious diseases. Prior knowledge of HLA polymorphisms in a population usually forms the basis for an effective case-control study design. As a prelude to future disease association ana...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2018.09.005
更新日期:2018-11-01 00:00:00
abstract::Haplotype V at the Y-chromosome specific DNA polymorphism (p49/TaqI) was reported in a study concerning 487 males originating from five different geographic locations in Iberia and North Africa. The highest frequency of haplotype V (68.9%) was previously observed in Berbers from Morocco, and it was previously establis...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(01)00287-7
更新日期:2001-09-01 00:00:00
abstract::A large percentage of healthy individuals (50-90%) is chronically infected with Cytomegalovirus (CMV). Over the past few years, several techniques were developed in order to monitor CMV-specific T-cell responses. In addition to the identification of antigen-specific T cells with peptide-loaded MHC complexes, most of t...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2004.02.012
更新日期:2004-05-01 00:00:00
abstract::The compatibility of HLA-A,-B expression between mother and offspring was examined in 410 families serologically tissue typed within a single transplant lab from 1972 thru 1982. The study group included 410 mothers of 1719 children (range 2-13/mother), with 352 one-father and 58 multiple-father families There were sev...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(84)90072-7
更新日期:1984-11-01 00:00:00
abstract::The occurrence of graft rejection episodes after orthotopic liver transplantation (OLT) despite the use of immunosuppressive drugs designed to suppress T lymphocyte functions, indicates the involvement of other types of cells in this process. The activity of natural killer cells and their killer immunoglobulin-like re...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2005.10.016
更新日期:2005-11-01 00:00:00
abstract::Human B cells nonspecifically activated by Epstein Barr virus (EBV) can present tetanus toxoid (TT) antigen to TT specific helper T cell lines and T cell clones. Presentation of TT antigen by EBV-B cells did not require the presence of TT specific B cells and did not involve B cell surface immunoglobulins. Immunosorbe...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(83)90039-3
更新日期:1983-11-01 00:00:00
abstract::Previously we have shown that the supratype HLA B17 BfS C4A6 B1 DR7 (17 6 1 7) carrying a C4/Bgl II RFLP correlating with C4A6 coexists in whites and Thai/Chinese. Using conventional and PFGE/Southern blotting with class II, class III, and TNF probes as well as serologic DQ subtyping, we have extended these comparison...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(88)90015-8
更新日期:1988-09-01 00:00:00
abstract::To identify disease-specific T cell changes that occur in Crohn's disease (CD) the T-cell receptor (TCR) BV repertoires of lamina propria lymphocytes (LPL) from both disease-active and disease-inactive colonic tissue of three CD patients were compared by a quantitative polymerase chain reaction (qPCR) and CDR3 length ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(96)00079-1
更新日期:1996-06-01 00:00:00
abstract::The human major histocompatibility complex includes a group of non-classical HLA class I genes, HLA-E, -F and -G. While nearly all focus since the discovery of these class Ib molecules have been on basic biochemistry and molecular biology of HLA-G and HLA-E, as well as their expression patterns, functions in immune mo...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2019.09.006
更新日期:2020-04-01 00:00:00
abstract::Cytomegalovirus (CMV) is a well-known cause of disease occurring after bone marrow transplantation (BMT). The manifestations of CMV range from asymptomatic infection, defined as active CMV replication in the blood in the absence of clinical manifestations or organ failure abnormalities, to CMV disease, characterized b...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2004.02.013
更新日期:2004-05-01 00:00:00
abstract::The 14-base pair (bp) polymorphism within the HLA-G gene has been investigated in heart transplant patients for the first time. The 14-bp polymorphism is associated with HLA-G mRNA stability and the patterns of alternative isoforms splicing, and therefore may influence the functionality of the HLA-G molecule. In heart...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.07.012
更新日期:2009-10-01 00:00:00
abstract::Complement receptor 1 (CR1/CD35) levels on erythrocytes and related CR1 polymorphisms have been associated with response to falciparum malaria in populations inhabiting malaria-endemic regions. Differences in disease association profiles of its low expression alleles have been observed in populations from different re...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2009.02.001
更新日期:2009-04-01 00:00:00
abstract::When lymphocytes of healthy donors were incubated with penicillin and washed before HLA typing, the cytotoxic reactivity of some typing sera with their corresponding antigen was blocked. When the penicillin was added to the cytotoxicity assay at different times, competition between penicillin and alloantiserum for bin...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(82)90033-7
更新日期:1982-08-01 00:00:00
abstract::Genomic aberrations in the CREBBP (CREB-binding protein - CREBBP or CBP) gene such as point mutations, small insertions or exonic copy number changes are usually associated with Rubinstein-Taybi syndrome (RTs). In this study, the disruption of the CREBBP gene on chromosome 16p13.3, as revealed by CGH-array and FISH, s...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2013.04.024
更新日期:2013-08-01 00:00:00
abstract::CD1 glycoproteins are a class of antigen presenting molecules that bind and present non-peptidic antigens (lipids and glycolipids) for immune recognition. CD1 polymorphisms, although limited, could have a critical role in antimicrobial, anticancer, and autoimmune responses and disease susceptibility. Ethnic difference...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2020.05.005
更新日期:2020-07-01 00:00:00
abstract::To clarify the molecular relationship between HLA loci and ulcerative colitis (UC) in Japanese patients, we performed HLA-DP genotyping by the PCR-RFLP method and studied tumor necrosis factor beta-chain genetic polymorphism by Southern hybridization, in addition to conventional serologic typing. Significant increase ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(93)90113-f
更新日期:1993-02-01 00:00:00
abstract::In humans, the in vitro antibody response directed towards mannan, a polysaccharide extracted from the cell wall of Candida albicans, has been previously shown to be dependent on the presence of T lymphocytes and monocytes. Evidence is now given for the existence of a genetic restriction governing this response since ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(86)90040-6
更新日期:1986-05-01 00:00:00
abstract::The purpose of this article is to study the amounts of gamma-aminobutyric acid-transaminase (GABA-t) during immune response in the human thymus. GABA-t was studied by biochemical and histochemical methods in 7 immunostimulated (treated) and 7 non-immunostimulated (untreated) patients (4 young adult, age range: 24-36 y...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(00)00130-0
更新日期:2000-07-01 00:00:00