Abstract:
BACKGROUND:Six human pancreatic carcinoma cell lines, designated as KMP-1 to KMP-6, were established and maintained in vitro for > 3 years. All were derived from pancreatic ductal adenocarcinomas. The six cell lines originated from either primary pancreatic tumors, metastatic liver tumors, or metastases to lymph nodes. METHODS:Each cell line was characterized by its morphology, doubling time, colony forming efficiency (CFE) on plastic dishes, tumorigenicity in nude mice, chromosomal analysis, and the amount of tumor markers secreted into the culture medium. Furthermore, mutations in the K-ras, p53, and p16/INK4a genes were analyzed. RESULTS:All cell lines grew as an adhering monolayer and were cultured in medium supplemented with 2% fetal bovine serum. The doubling time ranged from 16-70 hours, and the CFE ranged from 0.1-11%. Subcutaneous transplantation of these carcinoma cells into nude mice resulted in the formation of tumors. Chromosomal analysis showed that the modal numbers ranged from 43-124, and each karyotype was unique. Each cell line secreted detectable amounts of squamous cell carcinoma antigen, carcinoembryonic antigen, carbohydrate antigen 19-9, Dupan-II, and cytokeratin 19 fragment, respectively. Genetic alterations of the K-ras, p53, and p16 genes were detected in six, three, and five, respectively, of the six cell lines. CONCLUSIONS:The authors believe that these newly established pancreatic carcinoma cell lines will contribute to wide ranging studies regarding pancreatic carcinoma progression.
journal_name
Cancerjournal_title
Cancerauthors
Kato M,Shimada Y,Tanaka H,Hosotani R,Ohshio G,Ishizaki K,Imamura Mdoi
10.1002/(sici)1097-0142(19990215)85:4<832::aid-cncsubject
Has Abstractpub_date
1999-02-15 00:00:00pages
832-40issue
4eissn
0008-543Xissn
1097-0142pii
10.1002/(SICI)1097-0142(19990215)85:4<832::AID-CNCjournal_volume
85pub_type
杂志文章相关文献
CANCER文献大全abstract::To test the hypothesis that there is a positive association between chronic alcoholism and carcinoma of the pancreas, the mortality experience of 1382 chronic alcoholics was studied. Analysis was limited to a comparison of observed and expected proportional mortality of different causes of death in the 894 whites who ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(197509)36:3<1077::aid-cncr282036
更新日期:1975-09-01 00:00:00
abstract::Dr. Wendell G. Scott hailed mammography as the first significant contribution to the control of breast cancer since Halsted described the radical mastectomy in 1903. Mammography as engendered new approaches and has supplied new concepts of breast cancer. Through the tenacity of a few physicians convinced something cou...
journal_title:Cancer
pub_type: 历史文章,杂志文章
doi:10.1002/1097-0142(197511)36:5<1815::aid-cncr282036
更新日期:1975-11-01 00:00:00
abstract:BACKGROUND:Gastric carcinoids are uncommon, and are unlike carcinoids at other gastrointestinal sites, clinically and pathologically. METHODS:The authors studied specimens from 104 patients with gastric carcinoid, with study emphasis being placed on pathologic features, immunohistochemistry, clinical associations, and...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19940415)73:8<2053::aid-cncr2820
更新日期:1994-04-15 00:00:00
abstract::Several decades ago, it was reported that normal and malignant mesothelial cells were transformed into distinct cell types (epithelial to fibrous and fibrous to epithelial) when transferred to in vitro conditions. Those tissue culture data are still cited as evidence supporting that the mesothelial cell has multipoten...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19870801)60:3<334::aid-cncr28206
更新日期:1987-08-01 00:00:00
abstract:BACKGROUND:Little is known about cellular level genetic alterations in preinvasive breast lesions, particularly lobular carcinoma in situ. METHODS:We employed fluorescence in situ hybridization (FISH) using pericentromeric (alpha satellite) probes to assess numerical alterations of chromosomes 1, 7, 8, 16, 17, and X i...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0142(19960115)77:2<315::AID-CNC
更新日期:1996-01-15 00:00:00
abstract:BACKGROUND:Breast cancer is the second most common source of brain metastasis. Stereotactic radiosurgery (SRS) can be an effective treatment for some patients with brain metastasis (BM). Necrosis is a common feature of many brain tumors, including BM; however, the influence of tumor necrosis on treatment efficacy of SR...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.26314
更新日期:2012-03-01 00:00:00
abstract::Tissue specimens from 27 patients with mammary fibrocystic disease were assayed for estrogen (ER) and progestin (PR) receptors. Seventeen patients (63%) had ER-positive lesions (greater than 5 fmol/mg protein). A progression in ER presence was noted from fibrocystic disease (35%) and fibrosing adenosis (50%) to lobula...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19820615)49:12<2534::aid-cncr282
更新日期:1982-06-15 00:00:00
abstract::Breast cancer specimens from 600 women were assayed for estrogen receptors (ER) using an immunocytochemical assay (ICA) employing the monoclonal antiestrophilin antibody H222 Sp gamma. Results showed significant correlation with biochemical ER determinations as well as with tumor grade and menopausal status. In 449 ca...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19901015)66:8<1663::aid-cncr2820
更新日期:1990-10-15 00:00:00
abstract:BACKGROUND:Retroperitoneal lymph node dissection is a fundamental step in the surgical management of patients with pelvic gynecologic malignancies, but its applicability to geriatric patients is controversial. The objective of this study was to evaluate whether pelvic and aortic lymphadenectomy in elderly patients with...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(20011115)92:10<2562::aid-cncr160
更新日期:2001-11-15 00:00:00
abstract:BACKGROUND:Radiotherapy (XRT) is effective as the primary treatment modality for laryngopharyngeal cancer; however, complications of XRT can result in significant morbidity. Few previous studies have examined the effect of continued smoking on complications of XRT. The authors of this report hypothesized that patients ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.24499
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:The objectives of this study were to evaluate survival among current smokers, former smokers, and never smokers who are diagnosed with non-small cell lung cancer (NSCLC). METHODS:The study included patients who participated in the National Comprehensive Cancer Network's NSCLC Database Project. Current, form...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.27824
更新日期:2013-02-15 00:00:00
abstract::Radiation-induced small bowel injury is a limiting factor to postoperative tumoricidal pelvic doses exceeding 4500 to 5000 cGy. Data from a review of the literature showed the inadequacy of medical measures and the bad reproducibility of radiation therapeutic attempts to decrease small intestine damage. Recent studies...
journal_title:Cancer
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/1097-0142(19911215)68:12<2545::aid-cncr282
更新日期:1991-12-15 00:00:00
abstract::A Chinese patient with documented hepatocellular carcinoma (HCC) satisfied the criteria of Everson and Cole for spontaneous regression of malignant tumors. Subsequently he survived a tumor-free period of at least 13 years. During the period of regression, shrinkage of liver coincided with a rise of SGOT to a level com...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19820715)50:2<332::aid-cncr28205
更新日期:1982-07-15 00:00:00
abstract::A case of malignant eccrine poroma with lymph node metastases is described. The patient had a nodular growth of the right thigh 2 years before its first excision. Local recurrence in the scar occurred in 6 months, and by 8 months a right groin lymph node was involved. Subsequent skin nodules of the right thigh occurre...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/1097-0142(19840401)53:7<1579::aid-cncr2820
更新日期:1984-04-01 00:00:00
abstract::Granular cell tumor of the stomach is rare and may occur in association with similar tumors in the skin. Two such tumors were found in association with cutaneous granular cell tumors in two black females. Infiltrating carcinoma of the stomach was suspected at surgery in one case. All of the lesions were typical granul...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19840515)53:10<2104::aid-cncr282
更新日期:1984-05-15 00:00:00
abstract::Forty-three patients with widely metastatic melanoma were studied. Visceral metastases were present in 79% of the patient group, including five patients with brain metastases. No patients were excluded because of "early death," etc., from analysis. All 43 patients received 24-hour DTIC infusions. Dosages for individua...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19860601)57:11<2103::aid-cncr282
更新日期:1986-06-01 00:00:00
abstract:BACKGROUND:Neuroblastoma is the most common malignancy among infants, but risk factors remain poorly understood. Because most patients present in the first few years of life, it has been hypothesized that prenatal and perinatal exposures may contribute to the pathogenesis of neuroblastoma. METHODS:A population-based c...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.22486
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:Expression of neuroectodermal markers is a key feature of small cell lung carcinoma (SCLC). Although immune responses against a number of these proteins have been associated with paraneoplastic neuronal disease (PND), most patients with SCLC have anti-neuroectodermal antibodies in the absence of PND. Whether...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.21088
更新日期:2005-06-15 00:00:00
abstract:BACKGROUND:Preoperative chemoradiation improves survival in esophageal and gastroesophageal junction (GEJ) cancer. We evaluated irinotecan and cisplatin as induction chemotherapy followed by concurrent chemoradiation in esophageal cancer. METHODS:Patients with uT1N1M0 or uT2-4NanyM0 resectable squamous cancer or adeno...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.26591
更新日期:2012-06-01 00:00:00
abstract::After 12 cycles of MOPP therapy for Hodgkin's Disease, an adolescent black male developed hemorrhagic cystitis, which clinically and histologically was similar to that induced by cyclophosphamide. At that time he had a normal platelet count and had never been irradiated below the diaphram. The markedly contracted blad...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(197804)41:4<1261::aid-cncr282041
更新日期:1978-04-01 00:00:00
abstract:BACKGROUND:Activating mutations in RAS are frequently present in patients with acute myeloid leukemia (AML), but their overall prognostic impact is not clear. METHODS:A retrospective analysis was performed to establish the clinical characteristics of patients with RAS-mutated (RAS(mut) ) AML, to analyze their outcome ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.27596
更新日期:2012-11-15 00:00:00
abstract::In the evaluation of cervical cancer, computed tomography (CT) is used primarily to stage advanced tumors and evaluate for recurrence. Because of reports that CT imaging lacks sensitivity and specificity in the detection of primary tumors and metastatic lesions, its use in these applications has been limited, but rece...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/cncr.11684
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND:In a recently reported, randomized trial, it was found that a regimen of irinotecan once every 3 weeks for patients with advanced colorectal carcinoma was associated with a lower incidence of severe diarrhea compared with weekly treatment, and both regimens had similar efficacy. METHODS:Resource utilization...
journal_title:Cancer
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1002/cncr.20691
更新日期:2004-12-01 00:00:00
abstract::Clinical and histological correlates of survival in patients undergoing complete resection of pulmonary metastases from nonseminomatous testicular carcinoma were determined in 25 Stage C patients aged 17-38 years treated from 1969-1978. All patients had orchiectomy and retroperitoneal lymphadenectomy. Nineteen patient...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19830315)51:6<1152::aid-cncr2820
更新日期:1983-03-15 00:00:00
abstract::Some variant of mastectomy remains the mainstay of the treatment of the primary tumor for the vast majority of the 110,000 new cases of cancer of the breast which will be treated this year in the United States. Reconstruction of the breast after ablation for cancer has become an integral part of the therapeutic regime...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19840201)53:3+<809::aid-cncr2820
更新日期:1984-02-01 00:00:00
abstract:BACKGROUND:The assessment of pancreatic ductal adenocarcinoma (PDAC) response to therapy remains challenging. The objective of this study was to investigate whether changes in the tumor/parenchyma interface are associated with response. METHODS:Computed tomography (CT) scans before and after therapy were reviewed in 4...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.31251
更新日期:2018-04-15 00:00:00
abstract::The authors have performed a clinical and statistical analysis of the presenting features of adult acute leukemia classified according to the French-American-British (FAB) hematopathologic criteria. Observations were made on 70 variables of history, physical examination, and laboratory examination recorded for 195 pat...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19810515)47:10<2460::aid-cncr282
更新日期:1981-05-15 00:00:00
abstract:BACKGROUND:The influence of various clinicopathologic features on survival of patients with papillary thyroid carcinoma has been examined. In particular, histologic grade, which was obtained by a combined assessment of nuclear atypia, tumor necrosis, and vascular invasion (VAN score) was studied. According to this, his...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19931101)72:9<2680::aid-cncr2820
更新日期:1993-11-01 00:00:00
abstract::A retrospective study of 702 patients with clinically positive nodes associated with squamous cell carcinoma of the oral cavity, supraglottic larynx, and hypopharynx observed from 1954 to 1968 was done. The policies of treatment for the neck were not standardized during those years. Three hundred eleven patients who s...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(197702)39:2+<868::aid-cncr282039
更新日期:1977-02-01 00:00:00
abstract:BACKGROUND:Nipple duct lavage (NDL) may be useful for breast cancer risk stratification. Published guidelines recommend that women with a 5-year Gail risk >/=1.7% should consider undergoing lavage for any fluid-yielding ducts, but it is not known whether increased breast cancer risk or nipple fluid production predict l...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.22538
更新日期:2007-04-01 00:00:00