Abstract:
:The dctA gene, coding for the dicarboxylate transport protein, has an inducible promoter dependent on activation by the two-component sensor-regulator pair DctB and DctD. LacZ fusion analysis indicates that there is a single promoter for dctB and dctD. The dctA promoter is also induced by nitrogen limitation, an effect that requires DctB-DctD and NtrC. DctB alone is able to detect dicarboxylates in the absence of DctA and initiate transcription via DctD. However, DctA modifies signal detection by DctB such that in the absence of DctA, the ligand specificity of DctB is broader. dctAp also responds to heterologous induction by osmotic stress in the absence of DctA. This effect requires both DctB and DctD. A transposon insertion in the dctA-dctB intergenic region (dctA101) which locks transcription of dctA at a constitutive level independent of DctB-DctD results in improper signalling by DctB-DctD. Strain RU150, which carries this insertion, is defective in nitrogen fixation (Fix-) and grows very poorly on ammonia as a nitrogen source whenever the DctB-DctD signalling circuit is activated by the presence of a dicarboxylate ligand. Mutation of dctB or dctD in strain RU150 reinstates normal growth on dicarboxylates. This suggests that DctD-P improperly regulates a heterologous nitrogen-sensing operon. Increased expression of DctA, either via a plasmid or by chromosomal duplication, restores control of DctB-DctD and allows strain RU150 to grow on ammonia in the presence of a dicarboxylate. Thus, while DctB is a sensor for dicarboxylates in its own right, it is regulated by DctA. The absence of DctA allows DctB and DctD to become promiscuous with regard to signal detection and cross talk with other operons. This indicates that DctA contributes significantly to the signalling specificity of DctB-DctD and attenuates cross talk with other operons.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Reid CJ,Poole PSdoi
10.1128/JB.180.10.2660-2669.1998subject
Has Abstractpub_date
1998-05-01 00:00:00pages
2660-9issue
10eissn
0021-9193issn
1098-5530journal_volume
180pub_type
杂志文章abstract::Analysis of a region involved in the conjugative transfer of the lactococcal conjugative element pRS01 has revealed a bacteria] group II intron. Splicing of this lactococcal intron (designated Ll.ltrB) in vivo resulted in the ligation of two exon messages (ltrBE1 and ltrBE2) which encoded a putative conjugative relaxa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3531-3538.1996
更新日期:1996-06-01 00:00:00
abstract::The arginine biosynthetic precursors, ornithine, citrulline, and argininosuccinate, inhibit arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: soluble RNA ligase, adenosine monophosphate) activity in the in vitro attachment assay system. Ornithine is the most potent, argininosuccinate is next,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.589-591.1971
更新日期:1971-08-01 00:00:00
abstract::Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.13.4172-4178.1997
更新日期:1997-07-01 00:00:00
abstract::The glycan chains in peptidoglycan or murein are cross-linked by transpeptidation of the peptide side chains. To assess the fraction of side chains involved in cross-bridges, distinction has been made between cross-linkage and cross-linking. The first expression refers to the situation in unlabeled (or fully labeled) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.6.2028-2031.1992
更新日期:1992-03-01 00:00:00
abstract::The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.255-263.1982
更新日期:1982-01-01 00:00:00
abstract::Hydroxylamine mutagenesis was used to alter the tar gene that encodes the transmembrane Tar protein required for chemotaxis. Mutants defective in chemotaxis were selected, and the mutation was characterized by DNA sequencing. Two classes of mutations were found: nonsense and missense. The nonsense mutations were distr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.992-998.1986
更新日期:1986-09-01 00:00:00
abstract::The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.869-874.1981
更新日期:1981-09-01 00:00:00
abstract::Comamonas sp. strain CNB-1 degrades nitrobenzene and chloronitrobenzene via the intermediates 2-aminomuconate and 2-amino-5-chloromuconate, respectively. Deamination of these two compounds results in the release of ammonia, which is used as a source of nitrogen for bacterial growth. In this study, a novel deaminase wa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01762-06
更新日期:2007-04-01 00:00:00
abstract::This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.23.6908-6916.2001
更新日期:2001-12-01 00:00:00
abstract::Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1499-1503.2001
更新日期:2001-02-01 00:00:00
abstract::Whiteley, H. R. (University of Washington, Seattle) and N. G. McCormick. Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction. J. Bacteriol. 85:382-393. 1963.-At an acid pH, extracts of Micrococcus lactilyticus (Veillonella alcalescens) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.2.382-393.1963
更新日期:1963-02-01 00:00:00
abstract::Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4721-4729.1995
更新日期:1995-08-01 00:00:00
abstract::In Mexico, actinomycetoma is mainly caused by Nocardia brasiliensis, which is a soil inhabitant actinobacterium. Here, we report for the first time the draft genome of a strain isolated from a human case that has largely been found in in vitro and experimental models of actinomycetoma, N. brasiliensis HUJEG-1. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00210-12
更新日期:2012-05-01 00:00:00
abstract::Glutamine phosphoribosylpyrophosphate amidotransferase is stable in growing cells, but is inactivated in an oxygen-dependent process at various rates in starving or antibiotic-treated cells. On the basis of studies of the purified enzyme, we suggested (D.A. Bernlohr and R.L. Switzer, Biochemistry 20:5675-5681, 1981) t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.937-949.1983
更新日期:1983-02-01 00:00:00
abstract::Elongation factor P (EF-P) facilitates the translation of certain peptide motifs, including those with multiple proline residues. EF-P must be posttranslationally modified for full functionality; in enterobacteria, this is accomplished by two enzymes, namely, EpmA and EpmB, which catalyze the β-lysylation of EF-P at a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00722-18
更新日期:2019-05-08 00:00:00
abstract::Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00485-08
更新日期:2008-11-01 00:00:00
abstract::Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.17.5320-5323.2003
更新日期:2003-09-01 00:00:00
abstract::The antibiotic peptide nisin is the first known lantibiotic that uses a docking molecule within the bacterial cytoplasmic membrane for pore formation. Through specific interaction with the cell wall precursor lipid II, nisin forms defined pores which are stable for seconds and have pore diameters of 2 to 2.5 nm. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.10.3259-3261.2004
更新日期:2004-05-01 00:00:00
abstract::The gene GK3045 (741 bp) from Geobacillus kaustophilus HTA426 was cloned, sequenced, and overexpressed into Escherichia coli Rosetta (DE3). The deduced protein was a 30-kDa monomeric esterase with high homology to carboxylesterases from Geobacillus thermoleovorans NY (99% identity) and Geobacillus stearothermophilus (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01060-08
更新日期:2009-05-01 00:00:00
abstract::Cefsulodin lyses actively growing Escherichia coli by binding specifically to penicillin-binding proteins (PBPs) 1a and 1b. Recent findings (F. García del Portillo, M. A. de Pedro, D. Joseleau-Petit, and R. D'Ari, J. Bacteriol. 171:4217-4221, 1989) have linked cefsulodin-induced lysis to septation during the first div...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.1-5.1991
更新日期:1991-01-01 00:00:00
abstract::Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Mutations in gldN cause a partial defect in gliding. A novel bacteriophage selection strategy was used to aid construction of a strain with a deletion spanning gldN and the closely related gene gldO in an otherwise wild-type F. johnso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01495-09
更新日期:2010-03-01 00:00:00
abstract::The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.22.6948-6957.1999
更新日期:1999-11-01 00:00:00
abstract::The ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobU in Salmonella enterica). The latter activity is required for cobinamide salvaging in bacteria. This paper reports evidence that ar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.24.7193-7201.2003
更新日期:2003-12-01 00:00:00
abstract::Five clostridial species were found to ferment gluconate via 2-keto-3-deoxygluconate which subsequently is phosphorylated to yield 2-keto-3-deoxy-6-phosphogluconate (KDPG). This compound is then cleaved by KDPG aldolase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.570-573.1971
更新日期:1971-08-01 00:00:00
abstract::In a previous search for mutants of Salmonella typhimurium that are defective in heme synthesis, one class that is apparently defective in 5-aminolevulinic acid (ALA) uptake (alu) was found. Here, I describe the characterization of these mutations. The mutations all map to a single locus near 77.5 min on the genetic m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.325-331.1993
更新日期:1993-01-01 00:00:00
abstract::The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.445-448.1975
更新日期:1975-10-01 00:00:00
abstract::To examine the role of the amino acid residues (between positions 258 and 275 and positions 297 and 298) of the alpha-subunit of RNA polymerase in TyrR-mediated activation of the mtr promoter, we have carried out in vitro transcription experiments using a set of mutant RNA polymerases with a supercoiled mtr template. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.19.6187-6191.1997
更新日期:1997-10-01 00:00:00
abstract::Isolation of highly purified membrane fractions from phototrophically grown Rhodospirillum rubrum was achieved by velocity and isopyknic sedimentation under carefully controlled ionic conditions. Bacteriochlorophyll-rich and succinic dehydrogenase-rich chromatophores that were essentially devoid of contamination by no...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.3.1326-1338.1976
更新日期:1976-06-01 00:00:00
abstract::Ragland, T. E. (Brandeis University, Waltham, Mass.), T. Kawasaki, and J. M. Lowenstein. Comparative aspects of some bacterial dehydrogenases and transhydrogenases. J. Bacteriol. 91:236-244. 1966.-Twenty-eight diverse bacterial species were surveyed for the activities and coenzyme specificities of four enzymes: isocit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.236-244.1966
更新日期:1966-01-01 00:00:00
abstract::The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.1.290-301.2002
更新日期:2002-01-01 00:00:00