Identification and characterization of some PR-proteins induced by kitazin and Rhizoctonia solani causing sheath blight of rice.

Abstract:

:Significant reduction (68.38%) in sheath blight disease of rice was noticed when foliar spray of a systemic fungicide, kitazin (480 micrograms mg-1), was applied twice at an interval of 2 days before inoculation. SDS-PAGE analysis of proteins of Rhizoctonia-infected rice leaf sheaths revealed the presence of 16 proteins ranging from 20 to 90 kDa (approx.). Six were identified as constitutive defense proteins (increased after infection), 6 as secondary defense proteins (formed de novo) and the rest 4 appeared non-defense proteins. Non-inoculated kitazin-treated leaf sheaths showed 15 proteins of which 5 were constitutive and 4 secondary defense proteins (both are PR-proteins). Among the PR-proteins, five beta-1,3-glucanases and one chitinase was identified and characterized. One rice chitinase (MW 20 kDa) and 2 glucanases (60 & 69 kDa) showed serological relationships with tobacco chitinase (32 kDa) and tobacco glucanase (33 kDa) respectively. The implications of results have been discussed in relation to biotic and abiotic induction of PR-proteins in rice.

journal_name

Indian J Exp Biol

authors

Bera S,Purkayastha RP

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

644-9

issue

6

eissn

0019-5189

issn

0975-1009

journal_volume

35

pub_type

杂志文章
  • In vitro propagation of the Garden Heliotrope, Valeriana officinalis L.: influence of pre-chilling and light on seed germination.

    abstract::Valeriana officinalis is an important medicinal herb commonly found in Kashmir valley. This study forms an important preliminary step for in-vitro micro propagation of V. officinalis from breaking the seed dormancy, inducing rapid seed germination and its subsequent micro propagation. We investigated the influence of ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Bhat B,Sharma VD

    更新日期:2015-03-01 00:00:00

  • Lentiviral-mediated RNAi targeting p38MAPK ameliorates high glucose-induced apoptosis in osteoblast MC3T3-E1 cell line.

    abstract::The p38 mitogen activated protein kinase (p38MAPK) pathway is an important signaling cascade involved in cell growth, differentiation and apoptosis. High glucose activates p38MAPK pathway in different cells, including osteoblasts. In the present study, role of p38MAPK in high glucose induced osteoblast apoptosis and p...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Feng Z,Deng H,Du J,Chen D,Jiang R,Liang X

    更新日期:2011-02-01 00:00:00

  • Effect of gossypol on few testicular enzymes in mature rats.

    abstract::Effect of oral administration of gossypol acetic acid (15 mg/kg/day) for 10 weeks, on certain enzymes, which may be taken as markers for the different stages of spermatogenesis, was studied in male albino rats. Gossypol produced a significant decrease in hyaluronidase and sorbitol dehydrogenase, while no change was ob...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Nair IN,Bhiwgade DA

    更新日期:1989-12-01 00:00:00

  • Anti-fungal potentials of extracellular metabolites of Western Ghats isolated Streptomyces sp. NII 1006 against moulds and yeasts.

    abstract::Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Jayamurthy H,Valappil Sajna K,Dastagar SG,Pandey A

    更新日期:2014-11-01 00:00:00

  • Contribution of oxidative stress to radiosensitization by a combination of 2-DG and 6-AN in human cancer cell line.

    abstract::In the present studies, the role of oxidative stress in radiosensitization by a combination of 2-DG and 6-aminonicotinamide (6-AN) was examined in a human glioma cell line (BMG-1: wild type p53). Presence of 2-DG or 6-AN for 4 hr after irradiation (gamma ray 2.5 Gy) significantly enhanced the radiation-induced cell de...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Varshney R,Adhikari JS,Dwarakanath BS

    更新日期:2003-12-01 00:00:00

  • Biotin deficiency causes alterations in glycosaminoglycans and glycoproteins in rat aorta.

    abstract::Biotin deficiency produced by feeding raw egg white caused decrease in the concentration of many glycosaminoglycans (GAG) in the aorta in rats fed standard laboratory diet and atherogenic diet. This decrease has been observed to be due to the increased activity of enzymes involved in the degradation of GAG and glycohy...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Abraham A,Kurup PA

    更新日期:1993-02-01 00:00:00

  • Heat stress induced apoptosis in BC-8 cells derived from AK-5 tumor involves downregulation of Bcl-2 and generation of reactive oxygen species.

    abstract::BC-8, a rat histiocytoma undergoes apoptosis after heat shock, which is due to lack of an effective heat shock response. Heat shock induced generation of free radicals, which in turn are involved in the induction of apoptotic death in BC-8 cells. Treatment of BC-8 cells with N-acetylcysteine partially inhibited the he...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Khar A,Kumari AL,Pardhasaradhi BV,Varalakshmi Ch,Rangaraj N

    更新日期:2006-10-01 00:00:00

  • Synergistic effect of calcium stearate and photo treatment on the rate of biodegradation of low density polyethylene spent saline vials.

    abstract::The biodegradation of spent saline bottles, a low density polyethylene product (LDPE) by two selected Arthrobacter sp. under in vitro conditions is reported. Chemical and UV pretreatment play a vital role in enhancing the rate of biodegradation. Treated LDPE film exhibits a higher weight loss and density when compared...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Carol D,Karpagam S,Kingsley SJ,Vincent S

    更新日期:2012-07-01 00:00:00

  • Dietary requirement of tryptophan for growth and survival of the Indian major carp, Cirrhinus mrigala (Hamilton-Buchanan) fry.

    abstract::The experimental diets (40% protein) contained graded levels of tryptophan (0.60, 0.75, 1.00, 1.25, 1.50, 1.75%) of dietary protein. Each test diet was fed to triplicate groups of mrigal fry twice daily at 10% of the total biomass for 8 weeks. The dietary tryptophan requirement was estimated by plotting weight gain ag...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Benakappa S,Varghese TJ

    更新日期:2003-11-01 00:00:00

  • Antidepressant and anxiolytic-like effects of 4n, a novel 5-HT3 receptor antagonist using behaviour based rodent models.

    abstract::The present study was designed to investigate the putative antidepressant and anxiolytic-like effects of N-n-Butylquinoxalin-2-carboxamide (4n), a novel 5-HT3 receptor antagonist, with an optimal log P (2.01) and pA2 value (7.3) greater than ondansetron (6.9) using rodent behavioural models of depression and anxiety. ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Kumar B,Jindal A,Pandey DK,Bhatt S,Devadoss T,Mahesh R

    更新日期:2012-09-01 00:00:00

  • Effect of antioxidants on adenosine triphosphatase activity of brain and heart in male mice during ageing.

    abstract::The present study deals with the effect of butylated hydroxy anisole, propyl gallate and alpha-tocopherol on ATPase activity of brain and heart in ageing male mice. The activity of both the tissues significantly increased during pre-reproductive and reproductive phases. However, during the post-reproductive phase, ATP...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Garg SK,Khanna SC,Sharma SP

    更新日期:1992-04-01 00:00:00

  • Adaptogenic activity of Indian Panax pseudoginseng.

    abstract::The crude extract and saponins of Indian pseudoginseng and saponins of Korean ginseng have been studied using a battery of biological tests in rats and mice. Indian pseudoginseng saponins were found to exhibit better activity than the Korean ginseng saponins in several tests employed. The results indicate a need for i...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Dua PR,Shanker G,Srimal RC,Saxena KC,Saxena RP,Puri A,Dhawan BN,Shukla YN,Thakur RS,Husain A

    更新日期:1989-07-01 00:00:00

  • Effect of losartan and enalapril on cognitive deficit caused by Goldblatt induced hypertension.

    abstract::The present study was designed to evaluate the learning and memory, in an altered physiological state associated with increased blood pressure and activated renin angiotensin system in Wistar rats. The role of angiotensin in cognitive function was assessed by treatment with angiotensin converting enzyme (ACE) inhibito...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Srinivasan J,Jayadev S,Kumaran D,Ahamed KF,Suresh B,Ramanathan M

    更新日期:2005-03-01 00:00:00

  • Survivability of Salmonella paratyphi B var Java on experimentally infected cockroaches.

    abstract::Cockroaches are inhabitant of sewers and frequent visitors of kitchen and stores in the night to feed on left over. It is liable to disseminate a number of pathogens by contaminating kitchen surface, feeding vessels and food items left open. Salmonella paratyphi B var Java a common pathogen of man and animal was used ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Singh BR,Khurana SK,Kulshreshtha SB

    更新日期:1995-05-01 00:00:00

  • Activity of glutathione related enzymes and ovarian steroid hormones in different sizes of follicles from goat and sheep ovary of different reproductive stages.

    abstract::The investigations on enzymes related to glutathione like glutathione-S-transferase (GST) and glutathione peroxidase (GSH-Px) have been carried out mostly in human and rat ovaries, however the studies on these enzymes in ruminants are relatively absent. In the present study the changes in the activity of these enzymes...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sesh PS,Singh D,Sharma MK,Pandey RS

    更新日期:2001-11-01 00:00:00

  • Changes in rubisco content in relation to saccharides accumulation in wheat leaves during day.

    abstract::Two wheat varieties, T. durum (HD 4502) and T. aestivum (Kalyansona) were examined for photosynthesis rate and contents of sugars and rubisco protein in the flag leaf, at forenoon and afternoon at anthesis stage. A decrease in photosynthesis rate was observed in the afternoon compared to forenoon in both the varieties...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Rafique S,Sharma-Natu P,Ghildiyal MC

    更新日期:2001-11-01 00:00:00

  • Antimicrobial activity of crude extracts from plant parts and corresponding calli of Bixa orellana L.

    abstract::Ethanol extracts from the different parts of B. orellana showed differential antimicrobial activity. It was found that the extracts of in vitro leaves showed maximum activity against Bacillus pumilus followed by the extracts from the roots and hypocotyls. The callus derived from different explants too showed antimicro...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Castello MC,Phatak A,Chandra N,Sharon M

    更新日期:2002-12-01 00:00:00

  • Opioid peptides, opioid receptors and mechanism of down regulation.

    abstract::Biogenesis of various endogenous opioid peptides, anatomical distribution and the characteristics of multiple receptors with which they interact provides an opportunity for understanding the role of opioid systems and mechanism of opioid tolerance. Cellular and anatomical distribution of opioid receptor and their func...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章,评审

    doi:

    authors: Chaturvedi K

    更新日期:2003-01-01 00:00:00

  • Effect of estradiol-17beta on cell area, lumen area and trehalase activity of posterior silk gland of Bombyx mori L.

    abstract::Estradiol-17beta (E2) at the dose of 1 microg/g caused an increase in cell area, lumen area and the total (cell + lumen) area of posterior silk gland (PSG) in Bombyx mori indicating that exogenously applied estradiol-17beta has a regulatory influence on silk gland activity. A dose-dependent variation in trehalase acti...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Keshan B,Ray AK

    更新日期:2001-11-01 00:00:00

  • Circadian features of carbohydrate metabolism in domestic fowls exposed to weekly 120 degree-shifts of synchronizer schedule.

    abstract::Effects of weekly 8 hr advance- or delay-shifts on the circadian rhythm of plasma glucose, liver glycogen and muscle glycogen in male domestic fowls, beginning at about 3 days of age, were examined. Circadian rhythm in the aforesaid indices of carbohydrate metabolism in control birds was also studied. Blood and tissue...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Saini SK,Pati AK

    更新日期:1992-02-01 00:00:00

  • Alkaline lipase production by Citrobacter freundii IIT-BT L139.

    abstract::Around 150 lipase producing bacterial isolates were screened from the local soils enriched with oil. Citrobacrer freundii IIT-BT L139, an isolated microbial strain, produced lipase that had high activity (8.8 U/ml) at pH 9.0 and 40 degrees C. The 16S rDNA phylogenetic studies showed that Citrobacter freundii belongs t...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Gunasekaran V,Kotay SM,Das D

    更新日期:2006-06-01 00:00:00

  • Permeabilization and in situ adsorption studies during growth and coumarin production in hairy root cultures of Cichorium intybus L.

    abstract::Effect of addition of a permeabilizing agent dimethyl sulfoxide (DMSO) and a solid adsorbent, XAD -7, on growth and coumarin production in hairy root cultures of C. intybus was studied. Continuous permeabilization of the hairy root cultures of C. intybus with DMSO has been shown to be an effective strategy for enhance...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Bais HP,Sudha G,Suresh B,Ravishankar GA

    更新日期:2001-06-01 00:00:00

  • Aqueous extract of Ficus religiosa linn. reduces oxidative stress in experimentally induced type 2 diabetic rats.

    abstract::One of the major etiologies in pathogenesis of type 2 diabetes especially complications is oxidative stress. Aqueous extract of Ficus religiosa at a dose of 100 and 200 mg/kg orally decreased the fasting blood glucose in streptozotocin induced type 2 diabetic rats. The drug had enzyme induction effect with respect to ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Kirana H,Agrawal SS,Srinivasan BP

    更新日期:2009-10-01 00:00:00

  • Polyamines and plant alkaloids.

    abstract::Naturally occurring alkaloids are nitrogenous compounds that constitute the pharmacogenically active basic principles of flowering plants. Alkaloids are classified into several biogenically related groups. Tobacco alkaloids are metabolised from polyamines and diamines putrescine and cadaverine. N-methyl transferase is...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章,评审

    doi:

    authors: Ghosh B

    更新日期:2000-11-01 00:00:00

  • Chemical radiosensitization by misonidazole: production and repair of DNA single-strand breaks in Yoshida ascites tumor cells.

    abstract::Formation of strand-breaks in DNA and its repair in Yoshida ascites tumor cells exposed to gamma radiation (100-400 Gy) in presence and absence of misonidazole (10 mM) were studied. The methodology involved pre-labelling of cellular DNA by 3H-thymidine during cell proliferation in rats, irradiation of cells in vitro a...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Singh DR,Nair CK,Pradhan DS

    更新日期:1991-07-01 00:00:00

  • Radioiodide uptake and sodium iodide symporter expression in breast carcinoma.

    abstract::Breast cancer is a common malignancy in women all over the world and novel therapeutic approaches are required for the treatment of patients who become refractory to conventional therapies. Thyroid cancer is being treated successfully with radioiodine since many years. The iodide is transported inside the thyroid epit...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Damle AA,Narkar AA,Badwe RA

    更新日期:2011-06-01 00:00:00

  • Effect of caspase inhibitors on the post-thaw motility, and integrity of acrosome and plasma membrane of cryopreserved equine spermatozoa.

    abstract::The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spe...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Peter AT,Colenbrander B,Gadella BM

    更新日期:2005-06-01 00:00:00

  • Chloroplast ultra structure, photosynthesis and enzyme activities in regenerated plants of Stevia rebaudiana (Bert.) Bertoni as influenced by copper sulphate in the medium.

    abstract::Stevia rebaudiana (Bert.) Bertoni is an important medicinal plant used as noncaloric commercial sweetener. Plants regenerated with higher levels of copper sulphate in the medium exhibited enhanced activity of peroxidase and polyphenoloxidase (PPO) enzymes. Transmission electron microscopy (TEM) revealed increase in si...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Jain P,Kachhwaha S,Kothari SL

    更新日期:2014-09-01 00:00:00

  • Changes in Na and K ions in mouse liver following in vivo exposure to ultrasound.

    abstract::Mouse liver was exposed in vivo to a continuous and unfocussed ultrasound of 875 KHz at 5, 10 or 15 W/cm2 for 300 sec spread over five days and excised on day 0, 1, 5 and 10 for studying the changes in Na+ and K+ reserves and Na-K-ATPase activity. At 5 and 10 W intensities the change in ionic concentrations and Na-K-A...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Varma BM,Nath BS,Kumar RP

    更新日期:1994-10-01 00:00:00

  • Effect of 3-[p-(N-2-pyrimidylsulfamoyl)phenyl hydrazono]pentane-2,4-dione on blood parameters of albino rats.

    abstract::Intramuscular injection of 3-[p-(N-2-pyrimidylsulfamoyl)phenylhydrazono]pentane-2,4-dio ne (compound I) resulted in decrease in haemoglobin and alanine transaminase (ALT) and increase in aspartate transaminase (AST) values in albino rats. The serum glucose was found to decrease and after administration of 14 doses of ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Goyal RN,Garg DK

    更新日期:1996-10-01 00:00:00