Abstract：
:The p38 mitogen activated protein kinase (p38MAPK) pathway is an important signaling cascade involved in cell growth, differentiation and apoptosis. High glucose activates p38MAPK pathway in different cells, including osteoblasts. In the present study, role of p38MAPK in high glucose induced osteoblast apoptosis and potential of RNA interference (RNAi) targeting p38MAPK as a therapy strategy have been reported. Lentiviral-mediated RNAi effectively reduced p38MAPK and p-p38MAPK expressions in osteoblastic cell line (MC3T3-E1) following high glucose (22 mM) induction. Inhibition of p38MAPK activity significantly suppressed high glucose induced apoptosis of MC3T3-E1 cell and was confirmed by flow cytometry and ultra-structural examination by transmission electronic microscope. Inhibition of p38MAPK also significantly attenuates caspase-3 and bax protein expressions, but increased significantly bcl-2 expression as determined by Western blot analysis. The results suggested that p38MAPK mediates high glucose induced osteoblast apoptosis, partly through modulating the expressions of caspase-3, bax and bcl-2. Inhibition of p38MAPK with lentiviral-mediated RNAi or its specific inhibitor provides a new strategy to treat high glucose induced osteoblast apoptosis.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Feng Z,Deng H,Du J,Chen D,Jiang R,Liang Xsubject
Has Abstractpub_date
2011-02-01 00:00:00pages
94-104issue
2eissn
0019-5189issn
0975-1009journal_volume
49pub_type
杂志文章abstract：:Most phenolic substances of plant origin are toxic to microorganisms and they confer some degree of protection to plants against phytopathogens. Xanthomonas oryzae pv. oryzae, bacterial blight pathogen of rice (Oryza sativa) was treated with phenol (monohydroxy benzene) and its effects on the morphology and cytologica...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-02-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
更新日期：2002-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
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更新日期：2003-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:Aerobic and microaerobic diazotrophs possess numerous oxygen restriction strategies to protect nitrogenase from inactivation by oxygen without interfering with energy generation through oxidative phosphorylation. Protection by conformational change in nitrogenase was first detected and described in Azotobacter. This s...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1973-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-11-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
