Abstract:
:The genomic and cDNA sequences of BnSUT1C were isolated from B. napus. Combination of cDNA and genomic DNA sequences revealed that the BnSUT1C gene contained three exons and two introns. The cDNA encodes a protein of 513 amino acids with a calculated molecular mass of 54.7 kDa and an isoelectric point of 9.12. It exhibits typical features of sucrose transporter with 12 trans-membranes spanning domains. BnSUT1C showed highly homologous with AtSUC1 and AtSUC5. A histidine residue, which is conserved across all functional sucrose transporter proteins in higher plants, is located at position 66 of the BnSUT1C. Two putative pollen-specific cis-elements, AGAAA and GTGA motifs, are located in 5'-upstream of BnSUT1C. The spatial and temporal expression patterns carried out by semi-quantitative RT-PCR and Real-Time PCR, which indicated that BnSUT1C predominantly expressed in later developmental stages of anther, as tapetal cells began to shrink and collapse. BnSUT1C could mediate the uptake of sucrose in the pollen and retrieval of tapetal degenerated products during pollen maturation.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Li F,Yan L,Lai J,Ma C,Gautam M,Fu Tsubject
Has Abstractpub_date
2013-12-01 00:00:00pages
1130-6issue
12eissn
0019-5189issn
0975-1009journal_volume
51pub_type
杂志文章abstract::Urokinase-type plasminogen activator(uPA) assayed in uteri of cycling and deciduoma bearing rats shows detectable levels of this enzyme in endometrium. Zymographic analysis confirms uPA of decidual tissue and, following artificial induction of decidualisation, uPA activity constantly increases in the decidualising end...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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abstract::Getting higher yields of monoclonal antibody (MAb) is a problem in Hybridoma Technology which has two major bottlenecks--(a) poor yield of hybridized cells; and (b) low cellular productivity of MAb in culture. There are three ways of obtaining high MAb yield in vitro--(a) large scale culture of hybrid cells; (b) high ...
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pub_type: 杂志文章,评审
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
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doi:
更新日期:2012-10-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
abstract::Effect of chloride and diamide on testicular and epididymal angiotensin converting enzyme (ACE) activity was investigated using Hip-His-Leu as substrate in sheep. The chloride ions functioned as ACE activators, however, there was no linear correlation between the two. The optimum chloride concentrations were 500 mM fo...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2014-11-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:1989-08-01 00:00:00
abstract::In the present studies, the role of oxidative stress in radiosensitization by a combination of 2-DG and 6-aminonicotinamide (6-AN) was examined in a human glioma cell line (BMG-1: wild type p53). Presence of 2-DG or 6-AN for 4 hr after irradiation (gamma ray 2.5 Gy) significantly enhanced the radiation-induced cell de...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:2004-04-01 00:00:00
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更新日期:2003-01-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:1994-02-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2013-10-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2008-02-01 00:00:00
abstract::Breast cancer susceptibility gene (BRCA1) is known to be responsible for hereditary breast and ovarian cancer. This gene is highly penetrant conferring a risk for 0.92 by the age of 70. Germline mutation in this gene leads to susceptibility to breast and ovarian cancer, with a genotype phenotype correlation. Frequency...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2001-05-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
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更新日期:2006-07-01 00:00:00