Abstract：
:The hunt for an effective vaccine against malaria still continues. Several new target antigens as candidates for vaccine design are being explored and tested for their efficacy. In the present study the sera from mice immunized with 24,000 x g fraction of Plasmodium berghei has been used to identify highly immunogenic blood stage antigens. The protective antibodies present in immune sera were covalently immobilized on CNBr activated sepharose 4B and used for affinity chromatography purification of antigens present in blood stages of P. berghei. Two polypeptides of 66 and 43 kDa molecular weights proved to be highly immunogenic. They exhibited a strong humoral immune response in mice as evident by high titres in ELISA and IFA. Protective immunity by these two antigens was apparent by in vivo and in vitro studies. These two proteins could further be analysed and used as antigens in malaria vaccine design.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Pirta C,Banyal HSsubject
Has Abstractpub_date
2014-08-01 00:00:00pages
781-6issue
8eissn
0019-5189issn
0975-1009journal_volume
52pub_type
杂志文章abstract：:Present study was carried out in order to explore the ability of a bone graft-decalcified as well as fluoridated to form new bone when put in the rectus abdominis muscle of rabbit. A total of 66 implants placed inside the pouches created in rectus abdominis muscle of rabbits were followed for 2 to 20 weeks. Radiologic...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:Haloperidol, a butyrophenone used in the treatment of various psychoses has been clinically used and studied extensively. At the molecular level it is known to preferentially block D2 type of dopamine receptors but its other effects are unknown. We studied the effect of this drug on phospholipid biosynthesis in vitro ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:In the experimental group (shh inhibited group), there were significant decreases in the expression of Oct4, Nanog, Shh, GATA4, Brachyury and Goosecoid, while increases were observed for TAT and Pdx1. The expression of Sox17 did not differ between two control and experimental groups. In experimental group, the amount ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:Aqueous extract of leaves of M. oleifera was investigated and rationalised for its wound healing activity. The aqueous extract was studied at dose level of 300 mg/kg body weight using resutured incision; excision and dead space wound models in rats. Significant increase in wound closure rate, skin-breaking strength, g...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：:Aerobic and microaerobic diazotrophs possess numerous oxygen restriction strategies to protect nitrogenase from inactivation by oxygen without interfering with energy generation through oxidative phosphorylation. Protection by conformational change in nitrogenase was first detected and described in Azotobacter. This s...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-02-01 00:00:00
abstract：:N-formyl-methionyl-leucyl-phenylalanine (fMLP) a potent chemotactic peptide stimulates immune responses by activating macrophages and other cells of the immune system. The present study reports inhibition of fMLP-induced activation of murine peritoneal and P388D-1 macrophage cell line by protein kinase C (PKC) inhibit...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:Azadirachta indica (AI, Neem) was tested for analgesic potency in experimental pain models in mice. In the glacial acetic acid (GAA) induced writhing test, AI (10, 30 and 100 mg/kg) dose-dependently reduced both the incidence and the number of writhes. Similarly, AI, at the dose levels tested, also enhanced tail withd...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The implantation failure in newly inseminated mice induced by food deprivation for 48 hr, beginning at 0900 hrs on day 4 post coitum, was prevented by simultaneous exposure to light continuously for 48 or 36 hr. Food-deprived females that were exposed to continuous light for 36 hr showed a significant increase in feta...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Anther culture (AC) derived 160 doubled haploid (DH) families of an indica rice PTB 28 were field evaluated at Port Blair (lat 11 degrees.41'13.04"N; long 92 degrees 43'30.16"E), to assess the extent and range of induced variability among the androclones at segregating A2 generation and identify promising selectants f...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
abstract：:Mouse liver was exposed in vivo to a continuous and unfocussed ultrasound of 875 KHz at 5, 10 or 15 W/cm2 for 300 sec spread over five days and excised on day 0, 1, 5 and 10 for studying the changes in Na+ and K+ reserves and Na-K-ATPase activity. At 5 and 10 W intensities the change in ionic concentrations and Na-K-A...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Hydantoin derivatives, including phenytoin (5,5-diphenylhydantoin), have recently gained attention as they possess a variety of important biochemical and pharmacological properties. Nevertheless, available information on anticancer activity of hydantoin derivatives is still scarce. Here, we evaluated possible antileuk...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：:Tuberculin skin test (TST), an age old method is based on measuring delayed-type hypersensitivity (DTH) response to purified protein derivative (PPD). However, inspite of simplicity, ease and cost effectiveness, the usefulness of PPD test is limited due to its inability to distinguish among a protective immune respons...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2010-02-01 00:00:00
abstract：:Urokinase-type plasminogen activator(uPA) assayed in uteri of cycling and deciduoma bearing rats shows detectable levels of this enzyme in endometrium. Zymographic analysis confirms uPA of decidual tissue and, following artificial induction of decidualisation, uPA activity constantly increases in the decidualising end...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:Detached inflorescences of guar (Cyamopsis tetragonoloba), each bearing 4 uniformly-developing pods at 42 days post anthesis (DPA), were cultured for 6 days in complete liquid medium manipulated with a fixed concentration of mannose and varying concentration of myo-inositol. Such inflorescences, but with 2 pods, were ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:Study of antitumor and radiosensitizing properties of W. somnifera (Ashwagandha), a well known medicinal plant, have yielded encouraging results. The alcoholic extract of the dried roots of the plant as well as the active component withaferin A isolated from the extract showed significant antitumor and radiosensitizin...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
abstract：:Here, we investigated the quantitative and qualitative differences in antibody classes and subclasses in serum immune complexes (ICs) of Visceral Leishmaniasis (VL), Post Kala-azar Dermal Leishmaniasis (PKDL) and different cross reactive diseases like Malaria, Leprosy, Vitiligo as compared to control subjects. IC leve...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：:Anabas testudineus (2n = 46) had the more conserved pattern of its C-heterochromatin distributed mainly in the centromeric region, whereas Puntius sarana (2n = 50) exhibited a rather unorthodox pattern, many chromosomes showing interstitial, some telomeric and a few chromosomes showing centromeric C-band localization....
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Ruminal fungal isolates (Orpinomyces sp.; C-14, Piromyces sp.; C-15, Orpinomyces sp.; B-13 and Anaeromyces sp.; B-6), were evaluated under anoxic conditions for their effect on in vitro dry matter digestibility, neutral detergent fibre, acid detergent fibre and acid detergent lignin using rice and wheat straw as subst...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
abstract：:Administration of ethanol extract of stem bark from Z. rhoifolium (EEtOH-ZR) induced hypotension associated with a dual effect in heart rate in normotensive rats. This response was highlighted in spontaneously hypertensive rats (SHR). In rat superior mesenteric artery rings, the cumulative addition of EEtOH-ZR (0.1-75...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：:The Malnad region located in the Western Ghats of Karnataka is known for the cultivation of indigenous rain fed land race cultivar of rice. The present study was to investigate the callogenic and caulogenic potentialities of the two indigenous rice cultivar namely Karimundaga and Kanadatumba using dehusked mature embr...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2012-02-01 00:00:00
abstract：:Preventive role of lipistat against doxorubicin induced myocardial toxicity in rats has been reported. Cardiotoxicity was produced by doxorubicin administration (15 mg/kg for 2 weeks). Lipistat (350 mg/kg, orally) was administered as pretreatment for 2 weeks and then for 2 weeks alternated with doxorubicin. The genera...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Cells dissociated from spontaneous and transplanted tumours of C3HJax mammary gland have been cultured on polylysine and gelatin substrates. The isolated cells proliferated to form monolayers with high degree of organoid structure as indicated by formation of alveolar cavities. Differences were observed in the cell at...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Cells and organisms exposed to detrimental and toxic substances show different responses in photon emission dependent on amount, kind and exposure time of toxin as well as on the organism investigated. Radical reaction-generating substances and dehydrating, lipid dissolving and protein denaturating toxins which do not...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:The investigations on enzymes related to glutathione like glutathione-S-transferase (GST) and glutathione peroxidase (GSH-Px) have been carried out mostly in human and rat ovaries, however the studies on these enzymes in ruminants are relatively absent. In the present study the changes in the activity of these enzymes...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:Efficacy of vilva, a polyherbal formulation was evaluated in morphine induced constipated rats. Vilva juice, at a dose of 1.5 ml/100 g body wt was given orally for a period of 7 days. Morphine sulfate was injected to induce constipation on 8th day, 45 min before the experiments. Protein bound glycoconjungates were est...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
abstract：:Photons participate in many atomic and molecular interactions and processes. Recent biophysical research has discovered an ultraweak radiation in biological tissues. It is now recognized that plants, animal and human cells emit this very weak biophotonic emission which can be readily measured with a sensitive photomul...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：:Induction of differentially stained sister chromatids at G2/M and determination of baseline sister chromatid exchanges (SCEs) in ascites form of mouse sarcoma 180 cell line have been done by in vivo incorporation of 5-bromodeoxyuridine (BrdU) for two consecutive DNA replication cycles. The baseline SCE frequency is 6....
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:Estrogenic and antiestrogenic properties of E-492, a nonsteroidal compound (3 methyl-4'-(beta-pyrrolidinoethoxy)-2,3-diphenyl propiophenone), were assessed on the basis of ponderal, histologic, and biochemical changes in the uterus, cervix, and vagina of ovariectomized adult rats. This compound was studied at its max...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Effect of chronic treatment with hydralazine (40 mg/kg/day) on isolated heart, anococcygeus muscle and myometrial preparations from rats has been studied. The treatment for 6 weeks caused a significant increase in isoprenaline induced positive inotropic response in rat heart. However, isoprenaline induced positive chr...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:The present study on a defined deletion aroA mutant (B-26) of Salmonella enterica subspecies enterica serovar Abortusequi (S. Abortusequi) for residual virulence and safety in experimental model revealed that the virulence of the strain was at no difference in any of the cell assays (caprine alveolar macrophages, bovi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
