A method for large-scale multiplication of Curculigo orchioides through bulbil formation from leaf explant in shake flask culture.

Abstract:

:An efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (50 mgNL-1), benzyl adenosine (2.2 microM), adenine (0.11 mM), indole butyric acid (1.0 microM) and polyvinyl pyrrolidone (250 mgL-1). About 95% of explants produced maximum number of bulbils (546/flask at 6 weeks growth) in the medium. Shake flask cultures yielded 2737 bulbils/L medium whereas static cultures yielded 624 bulbils/L medium. Germination of bulbils was maximum (90.62%) on agar-gelled B5 medium containing benzyl adenosine (2.2 microM) and gibberellic acid (3.5 microM). Plantlets developed in vitro were successfully transferred to soil with a high rate of survivability (90%) and were comparable to natural population in growth and vigour.

journal_name

Indian J Exp Biol

authors

Suri SS,Arora DK,Ramawat KG

keywords:

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

145-8

issue

2

eissn

0019-5189

issn

0975-1009

journal_volume

38

pub_type

杂志文章
  • In vitro capacitation of goat spermatozoa.

    abstract::Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Anand SR,Atreja SK,Chauhan MS,Behl R

    更新日期:1989-11-01 00:00:00

  • Mycobacterial proteins--immune targets for antituberculous subunit vaccine.

    abstract::Cellular and humoral immunity induced by Mycobacterium tuberculosis has led to identification of newer vaccine candidates, but despite this, many questions concerning the protection against tuberculosis remain unanswered. Recent progress in this field has centered on T cell subset responses and cytokines that these ce...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章,评审

    doi:

    authors: Dhiman N,Khuller GK

    更新日期:1999-12-01 00:00:00

  • Hepato and reno protective action of Calendula officinalis L. flower extract.

    abstract::Flower extract of C. officinalis L. was evaluated for its protective effect against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. The activities of serum marker enzymes of liver injury like glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT) and alkaline phospha...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Preethi KC,Kuttan R

    更新日期:2009-03-01 00:00:00

  • Infrared spectroscopic analysis of tumor pathology.

    abstract::Infrared spectra of normal and malignant breast tissues were measured in the 600 cm(-1) to 4000 cm(-1) region. The measured spectroscopic features which are the spectroscopic fingerprints of the tissues contain the vital information about the malignant and normal tissues. Fourier Transform Infrared (FTIR) data on 25 c...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Mehrotra R,Gupta A,Kaushik A,Prakash N,Kandpal H

    更新日期:2007-01-01 00:00:00

  • Isolation, identification and characterization of secretory proteins of IVMFC embryos and blood circulation of estrus and early pregnant goat.

    abstract::The aim of the present study was to isolate, identify and characterize the secretory proteins of IVM oocytes and IVMFC embryos to evaluate its immunogenecity. and identify of such proteins if any, in blood circulation of estrus and early pregnant goats. Oocytes were matured in TCM-199 with 1 microg/ml, estradiol-17bet...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Malakar D,Majumdar AC

    更新日期:2005-08-01 00:00:00

  • Osteoinductive property of fluoride impregnated decalcified allogeneic bone matrix.

    abstract::Present study was carried out in order to explore the ability of a bone graft-decalcified as well as fluoridated to form new bone when put in the rectus abdominis muscle of rabbit. A total of 66 implants placed inside the pouches created in rectus abdominis muscle of rabbits were followed for 2 to 20 weeks. Radiologic...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Saraf SK,Tuli SM

    更新日期:1991-02-01 00:00:00

  • Impact of feeding ethanolic extract of root bark of Cananga odorata (Lam) on reproductive functions in male rats.

    abstract::The 50% ethanolic extract of the root bark of C. odorata administered orally at the dose of 1g/kg body weight/day for 60 days resulted in decreased epididymal sperm motility and sperm count in male albino rats. Morphological abnormalities were also observed in the sperms. The testicular glycogen, the activities of 3be...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Anitha P,Indira M

    更新日期:2006-12-01 00:00:00

  • Hepato protective effect of Liv-52 against CCl4 induced lipid peroxidation in liver of rats.

    abstract::Effect of oral feeding of Liv-52, on lipid peroxidation in normal liver and damaged liver induced by CCl4 of albino rats was studied. While Liv-52 did not show any effect on normal healthy liver cells, it had a significant protective effect against damage by CCl4 as shown by significant decrease in malonaldialdehyde c...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Pandey S,Gujrati VR,Shanker K,Singh N,Dhawan KN

    更新日期:1994-09-01 00:00:00

  • DNA extraction from crayfish exoskeleton.

    abstract::Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Li Y,Wang W,Liu X,Luo W,Zhang J,Gul Y

    更新日期:2011-12-01 00:00:00

  • Fishmeal extract bile salt lactose agar--a differential medium for enteric bacteria.

    abstract::Fishmeal extract bile salt lactose agar (FEBLA), a new differential medium for enteric bacteria was developed and evaluated for its ability to grow and differentiate lactose fermenters (LF) from non-lactose fermenters (NLF) in comparison with MacConkeys agar. Performance of FEBLA was at par with the latter. On FEBLA m...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Subbannayya K,Udayalaxmi J,Anugraha M

    更新日期:2006-08-01 00:00:00

  • gamma-Glutamyl transpeptidase activity in mice: age dependent changes and effect of cortisol.

    abstract::gamma-Glutamyl transpeptidase (gamma-GT) activity in various organs of mice during ontogenesis has been determined. The activity profile in foetus, neonates and the adult animal were compared to ascertain the functional significance of the enzyme. In addition, the effect of cortisol on gamma-GT activity in adult kidne...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Thusu N,Raina PN,Johri RK

    更新日期:1991-12-01 00:00:00

  • Hypersensitivity responses to Staphylococcus aureus infections in rabbits. An experimental study.

    abstract::Repeated weekly infections with S aureus, of bovine mammary origin, for three times evoked an immediate and a delayed type hypersensitivity in rabbits. The skin responses at 6 hr were characterized by oedema, haemorrhages and infiltration of polymorphonuclear cells. While at 24 hr it was dominated by mononuclear cells...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Varshney JP,Kapur MP,Sadana JR

    更新日期:1991-01-01 00:00:00

  • Cellular and molecular determinants of ageing.

    abstract::The highly complex nature of the process of ageing implicates both genetic and epigenetic causative factors. A progressive failure of maintenance underlines and typifies this process. The instability of the nuclear and the mitochondrial genomes is an important determinant of ageing. Infidelity and misregulation of gen...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章,评审

    doi:

    authors: Rattan SI

    更新日期:1996-01-01 00:00:00

  • Targeting folate metabolism for therapeutic option: A bioinformatics approach.

    abstract::Lymphatic filariasis, commonly called elephantiasis, poses a burden of estimated level of 5.09 million disability adjusted life year. Limitations of its sole drug, diethylcarbamazine (DEC) drive exploration of effective filarial target. A few plant extracts having polyphenolic ingredients and some synthetic compounds ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Hande S,Goswami K,Sharma R,Bhoj P,Jena L,Reddy MV

    更新日期:2015-11-01 00:00:00

  • Chloroplast ultra structure, photosynthesis and enzyme activities in regenerated plants of Stevia rebaudiana (Bert.) Bertoni as influenced by copper sulphate in the medium.

    abstract::Stevia rebaudiana (Bert.) Bertoni is an important medicinal plant used as noncaloric commercial sweetener. Plants regenerated with higher levels of copper sulphate in the medium exhibited enhanced activity of peroxidase and polyphenoloxidase (PPO) enzymes. Transmission electron microscopy (TEM) revealed increase in si...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Jain P,Kachhwaha S,Kothari SL

    更新日期:2014-09-01 00:00:00

  • In vitro antibacterial activity of Psidium guajava Linn. leaf extract on clinical isolates of multidrug resistant Staphylococcus aureus.

    abstract::In the present study, antibacterial activity of aqueous and organic extracts of Psidium guajava leaves was evaluated against multidrug resistant (MDR) clinical isolates of Staphylococcus aureus strains collected from hospitals in northern (Malabar region) Kerala. The strains which exhibited resistance against all the ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Anas K,Jayasree PR,Vijayakumar T,Manish Kumar PR

    更新日期:2008-01-01 00:00:00

  • Effect of silymarin on curcumin-induced mortality in zebrafish (Danio rerio) embryos and larvae.

    abstract::In presence of 7.5 microM of curcumin, no embryos or larva of zebrafish survived 3 days of incubation; however, coincubation with 144 microg/ml silymarin increased the survival rates of curcumin-treated embryos and larvae to about 70%. Moreover, in presence of 12.5 microM curcumin, all embryos died after 2 days of inc...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Shiau RJ,Shih PC,Wen YD

    更新日期:2011-07-01 00:00:00

  • Effect of tolmetin and its copper complex on wound healing.

    abstract::Dose matched comparison between copper complex of tolmetin(Tol-Cu) and tolmetin(Tol) was carried out in male albino rats bearing either sutured incision, dead space or excision wounds. Results showed that Tol significantly decreased tesile strength of incision and dead space wound. This effect was totally reversed by ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Somayaji SN,Jacob AP,Bairy KL

    更新日期:1995-03-01 00:00:00

  • Nimodipine is more effective than nifedipine in attenuating morphine tolerance on chronic co-administration in the rat tail-flick test.

    abstract::Opioids, when co-administered with L-type calcium channel blockers (L-CCBs) show morphine like higher antinociceptive effect. This antinociceptive effect has been further investigated using a different experimental paradigm. The effect of two different L-CCBs (nifedipine and nimodipine) on morphine-induced antinocicep...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ray SB,Mishra P,Verma D,Gupta A,Wadhwa S

    更新日期:2008-04-01 00:00:00

  • Biophotons from stressed and dying organisms: toxicological aspects.

    abstract::Cells and organisms exposed to detrimental and toxic substances show different responses in photon emission dependent on amount, kind and exposure time of toxin as well as on the organism investigated. Radical reaction-generating substances and dehydrating, lipid dissolving and protein denaturating toxins which do not...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sławinski J

    更新日期:2003-05-01 00:00:00

  • Ethanol potentiates in vivo hepatotoxicity of endosulfan in adult male rats.

    abstract::In an attempt to evaluate the effect and interaction of ethanol on endosulfan-induced hepatotoxicity in vivo to adult male rats, both, endosulfan (7.5 mg/kg body wt) and ethanol (1.5 g/kg body wt) were studied separately as well as in combination after a chronic oral exposure of 30 days. When fed separately, both the ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Singh SK,Pandey RS

    更新日期:1991-11-01 00:00:00

  • Effect of zinc on antioxidant response in maize (Zea mays L.) leaves.

    abstract::Maize (Zea mays L. cv kanaujia) plants grown with Zn [10 (control), 0.1 (low) and 20 microM (high)], were investigated for concentration of antioxidants and activities of antioxidative enzymes in leaves. Young leaves of low Zn plants developed whitish-necrotic spots. Leaves of both low and high Zn plants showed decrea...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Pandey N,Singh AK,Pathak GC,Sharma CP

    更新日期:2002-08-01 00:00:00

  • Effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity in rats.

    abstract::In order to find out the effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity, male rats fed with ethanol 1.6g/kg body weight per day for four weeks were studied. Besides a drastic reduction in body and testis weight, there was decrease in ascorbic acid, reduced g...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Maneesh M,Jayalekshmi H,Dutta S,Chakrabarti A,Vasudevan DM

    更新日期:2005-05-01 00:00:00

  • Inhibitory effect of cinnamoyl compounds against human malignant cell line.

    abstract::In the present study, anti-proliferative effects of dietary polyphenolic compounds have been observed and demonstrated the strong anticancer efficacy of curcumin (CMN), an active constituent of dietary spice (turmeric) using human leukemia cancer cell line. CMN inhibited the proliferation of K562 leukemic cells by ind...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Indap MA,Radhika S,Motiwale L,Rao KV

    更新日期:2006-03-01 00:00:00

  • Latitudinal variation in eclosion rhythm among strains of Drosophila ananassae.

    abstract::Eclosion rhythm parameters of D. ananassae strains originating between 8 degrees-34 degrees N were highly variable and latitude dependent. In the field under naturally fluctuating light intensity, temperature and R.H., the amplitude of the rhythm was high and the eclosion gate was narrow; however, under the naturally ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Joshi DS,Gore AP

    更新日期:1999-07-01 00:00:00

  • Effect of corticosterone on epididymal lipids in pubertal rat.

    abstract::Serum corticosterone excess was induced by the administration of corticosterone acetate to adrenal intact rats. Different lipid classes were studied in unwashed and washed (epididymal sperm and fluid free) caput and cauda epididymides. The unwashed caput epididymidis registered a significant decrease in total lipid, c...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Balasubramanian K,Arunakaran J,Srinivasan N,Aruldhas MM,Govindarajulu P

    更新日期:1989-08-01 00:00:00

  • Effect of aspirin on blood-lipid interaction and lipid peroxidation phenomena in relation to partition coefficient and biological activity.

    abstract::Considering the lipophilicity of aspirin (log P = -1.15), a significant contributor to its action mechanism, interaction of the drug with the whole lipids of goat blood have been investigated using phospholipid binding and lipid peroxidation phenomena as the parameters under investigation. The lipid content change alo...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Saha A,De AU,Sengupta C

    更新日期:2000-09-01 00:00:00

  • Effect of nutritional status on mutagenicity of urine excreted by rats treated with standard/experimental carcinogens.

    abstract::Urine samples, collected from Sprague Dawley rats treated with extracts of tobacco/masheri, benzo (a) pyrene, N'-nitrosonornicotine, N'-nitrosodiethylamine and maintained on semi-synthetic diets sufficient or deficient in Vitamin A, B and protein were tested for mutagenicity using Salmonella/microsome assay. The mutag...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ammigan N,Nagabhushan M,Nair UJ,Amonkar AJ,Bhide SV

    更新日期:1990-08-01 00:00:00

  • Studies on synergism between penicillins and ambodryl (bromodiphenhydramine HCl), an antihistamine with antimicrobial property.

    abstract::With respect to 31 different selected test bacteria, all sensitive to benzyl penicillin (Pc), ampicillin (Ap), methicillin (Me), ceporan (Ce), cloxacillin (Cx), streptomycin (Sm), kanamycin (Km), gentamicin (Gm), chloramphenicol (Cm), tetracycline (Tc), polymyxin (Pm) as well as ambodryl [Am; an antihistamine (bromodi...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ray S,Chattopadhyay D,Dastidar SG,Chakrabarty AN

    更新日期:1990-03-01 00:00:00

  • Effect of propofol in altering pentylenetetrazol induced seizure threshold in rats.

    abstract::The present study was undertaken to evaluate the role of propofol in altering pentylenetetrazol induced seizure threshold in rats. Total 42 Wistar rats were used to evaluate different parameters (onset of action, duration of seizure, seizure severity score and number of seizure) following propofol injection. The prese...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Prakash A,Medhi B,Puri A,Saikia B

    更新日期:2008-03-01 00:00:00