Abstract:
:An efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (50 mgNL-1), benzyl adenosine (2.2 microM), adenine (0.11 mM), indole butyric acid (1.0 microM) and polyvinyl pyrrolidone (250 mgL-1). About 95% of explants produced maximum number of bulbils (546/flask at 6 weeks growth) in the medium. Shake flask cultures yielded 2737 bulbils/L medium whereas static cultures yielded 624 bulbils/L medium. Germination of bulbils was maximum (90.62%) on agar-gelled B5 medium containing benzyl adenosine (2.2 microM) and gibberellic acid (3.5 microM). Plantlets developed in vitro were successfully transferred to soil with a high rate of survivability (90%) and were comparable to natural population in growth and vigour.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Suri SS,Arora DK,Ramawat KGkeywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
145-8issue
2eissn
0019-5189issn
0975-1009journal_volume
38pub_type
杂志文章abstract::Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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更新日期:1994-09-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:2008-04-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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journal_title:Indian journal of experimental biology
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更新日期:1999-07-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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journal_title:Indian journal of experimental biology
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doi:
更新日期:2000-09-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:1990-08-01 00:00:00
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journal_title:Indian journal of experimental biology
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