Abstract：
:Limb remote ischemic postconditioning (LRIP) can reduce ischemia-reperfusion injury (IRI), but its mechanisms are still unclear. We hypothesize that LRIP reduces IRI by reversing eNOS uncoupling. Focal ischemia was induced in Sprague-Dawley rats by middle cerebral artery occlusion for 2 h followed by a 24 h reperfusion. Before this surgery, folic acid (FA) was administered to the drug treatment group by gavage for 11 days. After a 24 h reperfusion, behavioural testing, vascular function, NO concentration and superoxide dismutase activity in the serum were determined. In addition, the infarct size of the brain was also detected. The mRNA of eNOS, nNOS, GTP cyclohydrolase I (GTPCH), P22(phox) and xanthine oxidase (XO) in the ischemic region were detected by RT-PCR, and nitrotyrosine (Tyr-NO2) was detected using Western blot analysis. The results showed that LRIP, FA and FA+LRIP all could improve behavioural score, and increase NO-mediated endothelium-dependent vasomotor responses, reduce infarction of rats subjected to IRI. Western blot and RT-PCR analyses showed that the Tyr-NO2 levels and the mRNA expression of NADPH oxidase catalytic subunit P22(phox) and XO were up-regulated in the ischemic brain, which was significantly inhibited by LRIP, FA and FA+LRIP. The mRNA expression of the rate-limiting enzyme in BH4 synthesis, GTPCH, was down-regulated in the ischemic brain, which could be significantly augmented by LRIP and FA+LRIP. It can be concluded that IRI induces eNOS uncoupling in the cerebral ischemic region and LRIP partially reverses the eNOS uncoupling induced by IRI.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Chen G,Yang J,Lu G,Guo J,Dou Ysubject
Has Abstractpub_date
2014-06-01 00:00:00pages
597-605issue
6eissn
0019-5189issn
0975-1009journal_volume
52pub_type
杂志文章abstract：:Anticancer potential of Moringa oleifera L. extracts have been well established. However, there are no reports on the isolated molecules/fractions from these extracts which are responsible for the anticancer/cytotoxic activity. Thus, in the present study, we explored the same. The n-hexane, chloroform, ethyl acetate, ...
journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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doi：
更新日期：1992-07-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：1990-04-01 00:00:00
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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更新日期：2000-01-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：2010-01-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：2005-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
更新日期：1996-03-01 00:00:00
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doi：
更新日期：2006-06-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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更新日期：2000-09-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
更新日期：2012-11-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2001-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2012-08-01 00:00:00
