Abstract：
:Around 150 lipase producing bacterial isolates were screened from the local soils enriched with oil. Citrobacrer freundii IIT-BT L139, an isolated microbial strain, produced lipase that had high activity (8.8 U/ml) at pH 9.0 and 40 degrees C. The 16S rDNA phylogenetic studies showed that Citrobacter freundii belongs to the family Enterobacteriaceae and later confirmed by the microbial identification. Suitable C and N sources for lipase production were deduced to be starch and peptone-urea, respectively. In a controlled fermenter (1 L), the lipase activity was found to increase by 36% (12 Uml(-1)). The variation of lipase activity, pH and dissolved oxygen (DO) during growth of the organism in the controlled batch fermenter were monitored. The rheological characteristics of the fermentation broth indicated that it behaved like a Newtonian fluid throughout the fermentation. The fermentation time was comparatively short (60 h). The lipase was also found to be substantially resistant to common detergents. This lipase was, thus, characterized as alkaline, thermostable and solvent stable, which was essentially desirable in pharmaceutical, detergent and other industrial applications or production.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Gunasekaran V,Kotay SM,Das Dsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
485-91issue
6eissn
0019-5189issn
0975-1009journal_volume
44pub_type
杂志文章abstract：:Most phenolic substances of plant origin are toxic to microorganisms and they confer some degree of protection to plants against phytopathogens. Xanthomonas oryzae pv. oryzae, bacterial blight pathogen of rice (Oryza sativa) was treated with phenol (monohydroxy benzene) and its effects on the morphology and cytologica...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Effect of oral feeding of Liv-52, on lipid peroxidation in normal liver and damaged liver induced by CCl4 of albino rats was studied. While Liv-52 did not show any effect on normal healthy liver cells, it had a significant protective effect against damage by CCl4 as shown by significant decrease in malonaldialdehyde c...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:The present study revealed the damaging effects of copper and hydrogen peroxide on DNA isolated from human blood in in vitro. Ultra violet spectral studies showed that copper and H2O2 alone (at 20 mM) caused destabilization of DNA structure. Notwithstanding, the effect was more prominent in combination of copper and H...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：:A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of posit...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Bioremediation is the most promising and cost effective technology widely used nowadays to clean up both soils and wastewaters containing organic or inorganic contaminants. Discharge of chromium containing wastes has led to destruction of many agricultural lands and water bodies. Utilisation of chromium(Cr) reducing m...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2003-09-01 00:00:00
abstract：:Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：:Drymaria cordata hydroethanolic extract (DCHE) at 25, 50 and 100 mg/kg (p.o.) was administered to study anxiolytic effect. Different models for anxiolytic activity viz. Hole board, Open field, Elevated plus maze, Light/dark exploration model were used. In the hole board model, there was dose dependent and significant ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：:Role of dietary energy and local ovarian hormones in conceptus development in the rat was evaluated by giving ip injections of estrogen and progesterone under diet restricted and/or dextrose supplemented conditions, using the experimental model of ovarian compensatory hypertrophy. Replacement of estrogen on day 2 and ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:A bioactive compound with antibacterial activity was isolated and purified from the extract of leaves of sunflower, Helianthus annuus. The bioactive compound was characterized using 1H and 13C NMR. The compound induced auxin, gibberellins and cytokinin in Oryza sativa and Phaseolus mungo. It also showed activity again...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:Azadirachta indica (AI, Neem) was tested for analgesic potency in experimental pain models in mice. In the glacial acetic acid (GAA) induced writhing test, AI (10, 30 and 100 mg/kg) dose-dependently reduced both the incidence and the number of writhes. Similarly, AI, at the dose levels tested, also enhanced tail withd...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Development of knowledge on lipids has attracted the scientific community for the effective utilization of the natural and synthetic lipids. Bioavailability of poorly water soluble drugs from gastrointestinal tract (GIT) can be enhanced by formulating the drugs in lipid based formulations. This formulation can increas...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2004-11-01 00:00:00
abstract：:Biogenesis of various endogenous opioid peptides, anatomical distribution and the characteristics of multiple receptors with which they interact provides an opportunity for understanding the role of opioid systems and mechanism of opioid tolerance. Cellular and anatomical distribution of opioid receptor and their func...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
abstract：:Indian toad (Bufo melanostictus, Schneider) skin extract (TSE) is pharmacologically potent and probably contains several bioactive compounds [Das et. al., Indian J Pharmacol, 28 (1996) 72]. A lethal factor was isolated and purified by neutral alumina column chromatography followed by HPLC. Spectroscopic (UV, IR, FAB-M...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：:Seeds of Ocimum sanctum L. (Labiatae; popularly known as 'Tulsi' in Hindi and 'Holy Basil' in English) contain a pale yellow colored fixed oil. The oil possesses antiinflammatory activity due to dual inhibition of arachidonate metabolism supplemented by antihistaminic activity. The antiinflammatory activity is not dep...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2007-05-01 00:00:00
abstract：:The present study deals with the effect of butylated hydroxy anisole, propyl gallate and alpha-tocopherol on ATPase activity of brain and heart in ageing male mice. The activity of both the tissues significantly increased during pre-reproductive and reproductive phases. However, during the post-reproductive phase, ATP...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:Tadpoles of B. melanostictus and R. cyanophlyctis were reared in vitamin A solution. Rearing of the tadpoles of B. melanostictus in solutions containing 15, 20 and 30 IU/ml vitamin A palmitate resulted in complete disappearance of the keratinized epidermal material over the jaws and horny labial teeth. However tadpole...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:Fluorescent pseudomonads were isolated from the rhizosphere of rice and sugarcane and examined for their biodiversity. All fifty strains of the fluorescent pseudomonads produced indole acetic acid. Among these pseudomonads, halves of sugarcane rhizosphere isolates and one isolate from the rice rhizosphere exhibited ph...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Ruminal fungal isolates (Orpinomyces sp.; C-14, Piromyces sp.; C-15, Orpinomyces sp.; B-13 and Anaeromyces sp.; B-6), were evaluated under anoxic conditions for their effect on in vitro dry matter digestibility, neutral detergent fibre, acid detergent fibre and acid detergent lignin using rice and wheat straw as subst...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
abstract：:A set of 25 Kanagawa(+) and Kanagawa(-) strains of V. parahaemolyticus was studied for their ability to degrade hydrocarbons in minimal media. All strains gave positive results with respect to crystal violet (CV), methyl violet, liquid paraffin, benzene, naphthalene and phenol. The CV double ring (CVDR) response had e...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:Doses of titanium trichloride (1/10th and 1/5th of LD50) were administered once and daily to pregnant rats to assess their effect on embryonic development. 1/5th dose of TiCl3 administered once orally on 1st, 2nd and 3rd day post-coitum. Similarly 1/10th of LD50 was administered daily. Results revealed that 1/10th LD5...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:Estradiol-17beta (E2) at the dose of 1 microg/g caused an increase in cell area, lumen area and the total (cell + lumen) area of posterior silk gland (PSG) in Bombyx mori indicating that exogenously applied estradiol-17beta has a regulatory influence on silk gland activity. A dose-dependent variation in trehalase acti...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:In vitro effects of various organophosphate pesticides (dimethoate, chlorpyrifos, ethion and monocrotophos) were studied on hemolysis, K+ leakage and lipid peroxidation in rat erythrocytes. All the four pesticides increased hemolysis and K+ leakage from erythrocytes, that was concentration and time dependent. On the c...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:Growth of C. tetragonoloba suffered with increase in concentration of Pb in soil. Plant biomass declined significantly at concentrations above 60 ppm of Pb. Roots showed more pronounced impact as compared to shoots. At highest applied concentration of lead (100 ppm), fresh weight of fruits decreased by 33% and dry wei...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:Bryan Standard strain of Rous Sarcoma Virus (BS-RSV) of subgroup A and pseudotype of Bryan high titre RSV(RAV-49) of subgroup C and an equal mixture of subgroup A and subgroup C virus were inoculated to 11-day old embryos of white leghorn (WL), Australorp (AL) and f1 and f2 generations of crosses between WL and AL bre...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Treatment of full-thickness wounds with A. vera, on rats resulted in increased biosynthesis of collagen and its degradation. A corresponding increase in the urinary excretion of hydroxyproline was also observed. Elevated levels of lysyl oxidase also indicated increased crosslinking of newly synthesised collagen. The r...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:In vitro treatment of erythrocytes of normal and hypercholesterolemic rats with N-nitrosodiethylamine (NDEA), an important carcinogen frequently present in human environment and food chain resulted in a marginal increase in osmotic fragility of erythrocytes without affecting their antioxygenic potential as evidenced b...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:Although peripheral axotomy of dorsal root ganglion cells in mice, rats and cats has been reported to generate spontaneous activity in sensory nerves, we did not find evidence for such activity in the hamster. In vitro, intracellular recording from L6-S1 dorsal root ganglion cells up to 6 weeks after axotomy did not r...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:Breast cancer is a common malignancy in women all over the world and novel therapeutic approaches are required for the treatment of patients who become refractory to conventional therapies. Thyroid cancer is being treated successfully with radioiodine since many years. The iodide is transported inside the thyroid epit...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：:Role of 5-HT3 receptors in cholinergic hypofunctional models of cognitive impairment in the elevated plus maze model and a passive avoidance model is studied. Cognitive impairment was caused by scopolamine (1 mg/kg, ip) in mice and 5-HT3 ligands mCPBG (1 and 5 mg/kg, ip) and ondansetron (0.5 and 5 mg/kg, ip) were admi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-02-01 00:00:00
