Abstract:
:The present study revealed the damaging effects of copper and hydrogen peroxide on DNA isolated from human blood in in vitro. Ultra violet spectral studies showed that copper and H2O2 alone (at 20 mM) caused destabilization of DNA structure. Notwithstanding, the effect was more prominent in combination of copper and H2O2. Further, agarose gel electrophoretic studies revealed that neither copper nor H2O2 alone had DNA fragmentation (up to 40 mM concentration), while copper and H2O2 together caused massive DNA fragmentation even at lower concentrations (4 mM copper + 4 mM H2O2). Therefore, it was concluded from the present study that the observed destabilization of DNA associated with alterations in configuration and subsequently massive DNA fragmentation was in response to copper and H2O2. Further, fluorescence spectroscopy and TUNNEL assay will address destabilization and fragmentation of naked DNA more precisely.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Prasad R,Kumar SR,Kumar Ssubject
Has Abstractpub_date
2006-05-01 00:00:00pages
377-80issue
5eissn
0019-5189issn
0975-1009journal_volume
44pub_type
杂志文章abstract::Doses of titanium trichloride (1/10th and 1/5th of LD50) were administered once and daily to pregnant rats to assess their effect on embryonic development. 1/5th dose of TiCl3 administered once orally on 1st, 2nd and 3rd day post-coitum. Similarly 1/10th of LD50 was administered daily. Results revealed that 1/10th LD5...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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更新日期:1998-01-01 00:00:00
abstract::Administration of single dose (175 mg/kg body wt) of amiodarone dissolved in water through gavage for 3 weeks damaged the lung and changed the content of lung lavage. Activities of bronchoalveolar lavage (BAL) angiotensin converting enzyme (ACE) and lactate dehydrogenase (LDH) increased significantly. Also, the protei...
journal_title:Indian journal of experimental biology
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doi:
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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abstract::Studies presented in this paper examined the tumor-specific cellular and humoral immunity induced by anti-idiotype antibodies (Ab2s) 2F10 and 3A4. A panel of Ab2s was made against a monoclonal anti-L1210/GZL lymphoma, 11C1. The Ab2s were screened for their ability to block 11C1 binding to tumor, to induce tumor-specif...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:1989-08-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::Toxic bait discs were prepared by incorporating the larvicide Abate (Temephos) technical, with yeast and dog biscuit (1:1), the latter forming the attractant source. These were assessed in 1, 10 and 25 litre aquaria and 100 litre drums against Aedes aegypti larvae. Floating as well as sinking bait discs were found equ...
journal_title:Indian journal of experimental biology
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更新日期:1996-03-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:2009-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2017-02-01 00:00:00
abstract::Protective effect of sex steroid hormones on aflatoxin-induced cytotoxicity on RBC (hemolysis) was examined in vitro. Addition of aflatoxin (2 micrograms/ml) in RBC suspension caused significant increase in hemolysis. Concurrent addition of aflatoxin (2 micrograms/ml) and estradiolbenzoate (100-1200 pg/ml) in RBC susp...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::Bipolar concentric electrodes were implanted in extreme lateral regions of hypothalamus having coordinates (A12.5 mm, L 3.5-3.7 mm, V 3.0-3.7 mm). These sites were electrically stimulated using biphasic square wave pulses (1 ms, 60 Hz) at a current strength ranging from 300-800 microA to evoke predatory attack on an a...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Ponderal, histological, and biochemical responses of the uterus, cervix, and vagina to the same dosage (1.5 mg/kg/day for 5 days) of cis- and transclomiphene were studied in ovariectomized rats in the presence and absence of estradiol-dipropionate (EDP). The results indicated that both isomers were estrogenic-cum-an...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1973-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2015-11-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Influence of piroxicam (PX) on glibenclamide (GL) induced hypoglycemia has been studied in rats, healthy human volunteers and diabetics. GL per se has significantly reduced blood sugar levels in rats and in humans. PX per se has significantly reduced BSLs, in diabetics, while having no significant influence on blood s...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Mechanisms of interleukin-18 (IL-18) and interleukin-10 (IL-10) in lipopolysaccharide (LPS) induced endotoxemia are not clear; their protective role is being investigated so that they may effectively modulate the host cytokine levels during endotoxemia. The aim of the study was to evaluate protective effects of IL-18 ...
journal_title:Indian journal of experimental biology
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doi:
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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doi:
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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更新日期:2002-12-01 00:00:00
abstract::The catfish C. batrachus were exposed to a long photoperiod of 14 hr light during resting--early preparatory (December-February) phases of the reproductive cycle. At 70-day sampling, both the seminal vesicle (SV) and testis registered marked stimulatory effects in comparisons to control fish maintained under approxima...
journal_title:Indian journal of experimental biology
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更新日期:1998-12-01 00:00:00
abstract::In the present study, antibacterial activity of aqueous and organic extracts of Psidium guajava leaves was evaluated against multidrug resistant (MDR) clinical isolates of Staphylococcus aureus strains collected from hospitals in northern (Malabar region) Kerala. The strains which exhibited resistance against all the ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Role of 5-HT3 receptors in cholinergic hypofunctional models of cognitive impairment in the elevated plus maze model and a passive avoidance model is studied. Cognitive impairment was caused by scopolamine (1 mg/kg, ip) in mice and 5-HT3 ligands mCPBG (1 and 5 mg/kg, ip) and ondansetron (0.5 and 5 mg/kg, ip) were admi...
journal_title:Indian journal of experimental biology
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doi:
更新日期:2002-02-01 00:00:00
abstract::In the present studies, the role of oxidative stress in radiosensitization by a combination of 2-DG and 6-aminonicotinamide (6-AN) was examined in a human glioma cell line (BMG-1: wild type p53). Presence of 2-DG or 6-AN for 4 hr after irradiation (gamma ray 2.5 Gy) significantly enhanced the radiation-induced cell de...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::The highly complex nature of the process of ageing implicates both genetic and epigenetic causative factors. A progressive failure of maintenance underlines and typifies this process. The instability of the nuclear and the mitochondrial genomes is an important determinant of ageing. Infidelity and misregulation of gen...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::A new gamma-aminobutyric acid derivative, N-phthaloyl GABA (P-GABA), was synthesised and its anticonvulsant activity was tested and compared with sodium valproate for efficacy against experimentally induced convulsions in mice. At a dose of 80 mg/kg, P-GABA rendered more protection than sodium valproate. ED50 of P-GAB...
journal_title:Indian journal of experimental biology
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doi:
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2000-03-01 00:00:00