Abstract：
:In the present study, antibacterial activity of aqueous and organic extracts of Psidium guajava leaves was evaluated against multidrug resistant (MDR) clinical isolates of Staphylococcus aureus strains collected from hospitals in northern (Malabar region) Kerala. The strains which exhibited resistance against all the antibiotics tested was selected for antibacterial assays. Minimum inhibitory concentration (MIC) for methanolic and aqueous extracts was found to be 625 ug/ml and 7.5 mg/ml, respectively. Minimum bactericidal concentration (MBC) recorded for methanolic and aqueous extracts was 1.25 and 12.5 mg/ml, respectively. Methanolic extract at minimum bactericidal concentration inhibited the growth of MDR strain by 80%. Time-kill assay revealed that methanolic extract (4 mg/ml) killed MDR bacteria within 10 hr. Total polypeptide profiling of bacterial cultures by SDS-PAGE indicated a high degree of protein degradative activity of the extract. Finally, a human RBC based haemolytic assay showed absence of haemolysis even at concentrations higher than that of MBC, advocating thereby its safety in therapeutic use.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Anas K,Jayasree PR,Vijayakumar T,Manish Kumar PRsubject
Has Abstractpub_date
2008-01-01 00:00:00pages
41-6issue
1eissn
0019-5189issn
0975-1009journal_volume
46pub_type
杂志文章abstract：:The Vero cell culture adapted buffalo pox virus was found to be completely attenuated at 40th passage for rabbits as well as buffaloes since it did not produce any thermal reaction or skin lesions. It induced high level of humoral and cell mediated immune response in rabbits as well as buffaloes. The antibody titres o...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:The epithelial and mesothelial cellular components of organs can be obtained as dissociated cells using adequate procedures of enzymatic digestion followed by pycnotic separation on density gradients. Using a specially developed procedure for tissue dissociation, the epithelial and connective tissue components of endo...
journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:The present study is planned to assess the diagnostic relevance of silver stained nucleolar organizer regions (NORs) in discriminating benign from malignant lesions of the breast and to ascertain the interobserver variability in the enumeration of AgNOR counts. Forty four specimens of benign and malignant breast tissu...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：2004-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
更新日期：2008-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
