Paraffin baiting system for demonstration of growth and biofilm production in Pseudomonas aeruginosa.

Abstract:

:Pseudomonas aeruginosa is one of the commonest pathogens among the pseudomonads. This organism can grow in minimal nutritional requirements. Because of the ability of pseudomonads to grow on paraffin is not commonly found among other human pathogens and the primary human pathogen being P. aeruginosa, we studied the adaptation of this organism to paraffin baiting system for growth and biofilm formation. Strains were tested for the capacity to use paraffin as the sole source of carbon using Czapek's minimal salt medium. Of the 53 clinical isolates of P. aeruginosa, 20 strains exhibited growth by 24 hrs and 42 strains by 48 hrs. The remaining strains did not show any growth in the paraffin baiting system. The oxidase test with the paraffin baiting system was also performed. This simple and inexpensive method can be used to isolate and demonstrate the biochemical and biofilm forming capacity of the organism.

journal_name

Indian J Exp Biol

authors

Vishnu Prasad S,Ballal M,Shivananda PG

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

258-60

issue

3

eissn

0019-5189

issn

0975-1009

journal_volume

45

pub_type

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