Abstract:
:Seed characteristics and in vitro culture of C. tamala embryos were studied. Embryos desiccated below 50% (fresh weight) exhibited poor morphogenetic response in vitro and confirmed the recalcitrant nature of seeds. The immature embryos of various developmental ages (4-16 week after flowering, WAF) were cultured on different strengths of MS medium. Morphogenesis responses were recorded after 10 days of culture. The best culture responses were achieved from the immature embryos of 12 WAF on MS medium with sucrose (3%, w/v), polyvinyl pyrollidone (100 mg L(-1)) and benzyl adenine (12 microM). Under optimum condition -60% explants responded; and -7.3 shoots buds developed per explants after 35 days of culture initiation. The shoot buds could be converted into micro-shoots on MS medium with sucrose (3%) and kinetin (3 microM). About 5.3 micro-shoots/shoot buds sprouted per sub-culture. The micro-shoots were rooted by maintaining them on MS medium with alpha-naphthalene acetic acid (3 microM) where within 6-8 wk of culture -8-10 roots developed. The rooted plantlets were acclimatized in vitro before they were transferred to community potting mix and maintained in the poly-shade ca 75% shading. The transplants registered -70% survival after two months of transfer.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Deb MS,Jamir NS,Deb CRsubject
Has Abstractpub_date
2014-10-01 00:00:00pages
1003-10issue
10eissn
0019-5189issn
0975-1009journal_volume
52pub_type
杂志文章abstract::Rate of heat production during cell proliferation following UV-irradiation of respiratory-deficient yeast cells was measured as a function of time (p-t curve) in a batch microcalorimeter. Following observations were made: (a) All growing cell cultures showed 3 distinct phases of heat production namely lag, exponential...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::FtsZ plays an important role in bacterial cell division by polymerizing to form the Z ring at the site of cytokinesis. Phytochemicals are known to disrupt bacterial cell division through inhibition of FtsZ assembly. In the present study phytochemicals like eugenol, trans-cinnamic acid, 4-formyl cinnamic acid, naringen...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::Enzymed-linked immunosorbent assay of hilsa and pomfret muscle extracts showed specific IgE binding to ten allergic patients' sera, the results corroborated to that of skin prick test. Comparison of allergen profiles of the two fish extracts by immunoblotting revealed a common antigenic protein of 50 kDa and some high...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Ricin, a glycoprotein from castor oil seeds, is specifically toxic to Kupffer cells and at low doses it leaves parenchymal cells comparatively unaffected. At a dose of approximately 1.5 microgram/100 g body weight, ricin significantly increases the hepatic antioxidant enzyme system in rats within 24 hr. Superoxide dis...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Effect of oral administration of gossypol acetic acid (15 mg/kg/day) for 10 weeks, on certain enzymes, which may be taken as markers for the different stages of spermatogenesis, was studied in male albino rats. Gossypol produced a significant decrease in hyaluronidase and sorbitol dehydrogenase, while no change was ob...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::Primary regenerants (1190) of a tall traditional salt tolerant rice cultivar pokkali were produced through in vitro culture from mature seed derived calli of fourth subculture. Out of 35000 SC2 regenerants, 26 promising lines with superior agronomic traits were chosen initially for evaluation. SC3 and SC4 generations ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of posit...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Effect of pulse treatments of cordycepin, an analog of adenosine, on S and G2 phases of the cell cycle of the mitotically synchronous plasmodia of Physarum polycephalum has been studied. Various concentrations of the drug (50-200 micrograms ml-1) were found to be effective in delaying mitosis by several hours in both ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::Although peripheral axotomy of dorsal root ganglion cells in mice, rats and cats has been reported to generate spontaneous activity in sensory nerves, we did not find evidence for such activity in the hamster. In vitro, intracellular recording from L6-S1 dorsal root ganglion cells up to 6 weeks after axotomy did not r...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Effects of ampicillin (Amp) in combination with riboflavin on septic arthritis in mice infected with Staphylococcus aureus have been reported. Ampicillin was given at 100 mg/kg after 24 h of infection, followed by riboflavin (Ribo) at 20 mg/kg body wt, after 2 h of Amp treatment. Mice were sacrificed at 3, 9, 15 days ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract::Aqueous extract of leaves of M. oleifera was investigated and rationalised for its wound healing activity. The aqueous extract was studied at dose level of 300 mg/kg body weight using resutured incision; excision and dead space wound models in rats. Significant increase in wound closure rate, skin-breaking strength, g...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract::Mouse liver was exposed in vivo to a continuous and unfocussed ultrasound of 875 KHz at 5, 10 or 15 W/cm2 for 300 sec spread over five days and excised on day 0, 1, 5 and 10 for studying the changes in Na+ and K+ reserves and Na-K-ATPase activity. At 5 and 10 W intensities the change in ionic concentrations and Na-K-A...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Ethanol extracts from the different parts of B. orellana showed differential antimicrobial activity. It was found that the extracts of in vitro leaves showed maximum activity against Bacillus pumilus followed by the extracts from the roots and hypocotyls. The callus derived from different explants too showed antimicro...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::Ten isoleucine+valine and three leucine auxotrophs of Sinorhizobium meliloti Rmd201 were obtained by random mutagenesis with transposon Tn5 followed by screening of Tn5 derivatives on minimal medium supplemented with modified Holliday pools. Based on intermediate feeding, intermediate accumulation and cross-feeding st...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disease characterized by the selective loss of dopamine (DA) neurons of the substantia nigra pars compacta (SNc). The events, which trigger and/or mediate the loss of nigral DA neurons, however, remain unclear. Neuroleptic-induced catalepsy has long been used as an anima...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Estradiol-17beta (E2) at the dose of 1 microg/g caused an increase in cell area, lumen area and the total (cell + lumen) area of posterior silk gland (PSG) in Bombyx mori indicating that exogenously applied estradiol-17beta has a regulatory influence on silk gland activity. A dose-dependent variation in trehalase acti...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Cyclooxygenase (COX) isoenzyme is known to play an important role in the pathophysiology of Parkinson's disease. The present study evaluated the neuroprotective effect of nimesulide, a preferential COX-2-inhibitor against 1-methyl-4-phenyl-1,2,3,6-tertahydropyridine (MPTP)-model of Parkinson's disease. Intrastriatal a...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract::Biochemical estimation of acidic and basic proteins of chick gastrocnemii (G. externus, G. medius and G. internus) and pectoralis muscles has been done under normal, denervated and work stress conditions from 1-56 days of postnatal growth. The reciprocal relationship of the two protein groups is clearly established. I...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::An unusual nucleolar organizer region (double NOR) on chromosome 13 was observed in a Down syndrome child [47, XY, +21, dNOR(13)]. The variant chromosome was inherited from the mother [46, XX, dNOR(13)]. The extra chromosome 21 in the proband was maternal origin. The frequency of NOR chromosome association showed rela...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract::The present study was undertaken to identify the receptor for dengue virus type 2 (DV) induced macrophage cytotoxin (CF2) on mouse peritoneal macrophages (MPhi). The binding of 125I-labelled CF2 to MPhi was saturable (15 nM), reversible, temperature, pH- and time-dependent. The saturation concentration was similar to ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::Following treatment, cypermethrin and fenvalerate, were found to have inhibitory effect on serum acetylcholine esterase (AchE) activity of cattle and buffalo experimentally infested with B. microplus. The pattern of AchE activity in infested-pyrethroid-treated group was found to be significantly different from either ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::Studies presented in this paper examined the tumor-specific cellular and humoral immunity induced by anti-idiotype antibodies (Ab2s) 2F10 and 3A4. A panel of Ab2s was made against a monoclonal anti-L1210/GZL lymphoma, 11C1. The Ab2s were screened for their ability to block 11C1 binding to tumor, to induce tumor-specif...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:1989-08-01 00:00:00
abstract::Dianex, a polyherbal formulation intended to use for diabetic patients, has been screened for toxic effects. For acute toxicity studies, Dianex was administered orally in graded doses of 0.75-10 g/kg to the mice. For subacute toxicity studies, different doses of Dianex (1.0, 1.5 and 2.5 g/kg) were administered orally ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::Leprosy bacillus (LB) and leprosy derived in vitro culture forms, the chemoautotrophic nocardioform (CAN) bacteria, showed an extremely close homology and identity with each other as regards a chemoautotrophic nutritional pattern, a nocardioform morphology, a weak acid-fastness coupled with Gram and Gomori's stain pos...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2001-10-01 00:00:00
abstract::Chemical composition of inter and intracellular hyphae was elucidated in Pennisetum pedicellatum roots colonized by Gigaspora margarita by employing energy dispersive X-ray microanalysis. Maximum amount of phosphate was estimated in polyphosphate granules present in the roots. Amount of phosphate was low in fungal hyp...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Effects of steroid hormones on beta-glucuronidase activities of granulosa cells and theca interstitial cells were studied in vitro in the presence and absence of cholesterol in minimum essential medium (MEM with Hank's salts). Conspicuous fall in the enzyme activities of both these cells were noticed during first 10 m...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::In malaria, development of resistance towards artemisinin derivatives has urged the need for new drugs or new drug combinations to tackle the drug resistant malaria. We studied the fresh root extract of Vetiver zizanioides (Linn.) Nash (VET) with a CDRI-CIMAP antimalarial α/β arteether (ART) together for their antimal...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2016-05-01 00:00:00
abstract::Pups (5 days old) were undernourished by separating them for 14 hr daily from their mothers for 7, 10, 13, 16 and 20 days. The undernourished rats showed significant decrease in body and brain weight, protein and nucleic acid contents at all stages of observation as compared to controls. The activities of SDH and AChE...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-09-01 00:00:00
abstract::Activities of several drug metabolising enzymes in the small intestine were investigated in Swiss mice, Sprague Dawley rats and Syrian Golden Hamsters fed 10% masheri, a pyrolysed tobacco product, in diet, for 20 months. The basal levels of enzymes in proximal (PI), medium (MI) and distal (DI) parts of the intestine i...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00
abstract::There was a significant increase in fucose (52%), total hexoses (16%) and hexosamine (56%) except sialic acid, which was reduced (77%) in the microvillus membrane of infants born to rat mothers made diabetic by injecting alloxan on day 3 of gestation. Expressed on the protein basis there were a significant increase in...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00