Identification of allergens in Indian fishes: hilsa and pomfret exemplified by ELISA and immunoblotting.

Abstract:

:Enzymed-linked immunosorbent assay of hilsa and pomfret muscle extracts showed specific IgE binding to ten allergic patients' sera, the results corroborated to that of skin prick test. Comparison of allergen profiles of the two fish extracts by immunoblotting revealed a common antigenic protein of 50 kDa and some high molecular weight fish allergens instead of low molecular weight parvalbumin found in several fishes. Purified and well characterized fish allergens are always considered better than crude fish extracts for diagnostic use.

journal_name

Indian J Exp Biol

authors

Das A,Chakraborti P,Chatterjee U,Monddal G,Chatterjee BP

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

1170-5

issue

12

eissn

0019-5189

issn

0975-1009

journal_volume

43

pub_type

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