Leprosy bacillus--possibly the first chemoautotrophic human pathogen cultivated in vitro and characterised.

Abstract:

:Leprosy bacillus (LB) and leprosy derived in vitro culture forms, the chemoautotrophic nocardioform (CAN) bacteria, showed an extremely close homology and identity with each other as regards a chemoautotrophic nutritional pattern, a nocardioform morphology, a weak acid-fastness coupled with Gram and Gomori's stain positivity, an exclusive mycolate and lipid profile, a phenolic glycolipid (PGL-I) and a highly sequestrated DNA characteristic, namely, a unique small size, a low G+C % mole, an exceptionally high gamma and UV radiation resistance, and a high thermal resistance. LB/CAN bacteria (CANb) gave positive signals for 36 kDa protein PCR, as well as, for 65 kDa epitope, and hybridisation with two or more probes and also by RFLP-analysis. Both LB/and CAN bacteria exhibited bacillary multiplication in the mouse footpads (MFP), nerve infiltration and evidences for local pathogenicity associated with pronounced systemic invasion. A highly reproducible mutilation model could be established which enabled a successful application of the postulates of Koch. The proof of their total identity was their anergic reactions in LL cases counterpoised against Mitsuda type strong nodular responses, mirroring the reactions of leprosy bacilli in TT cases, in accordance with the dictum of XIth International Leprosy Congress (1978). Thus, the chemoautotrophic nutritional requirements of LB, entirely unsuspected for a medically important pathogenic bacterium, having dimorphic (both bacillary and mycelial) characters with spores, mycelia and granules and unique pathogenicity of multilation manifested through the virulence factor, the enzyme collagenase, made LB or M leprae the highly enigmatic bacterium for so long.

journal_name

Indian J Exp Biol

authors

Chakrabarty AN,Dastidar SG,Sen A,Banerjee P,Roy R

keywords:

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

962-83

issue

10

eissn

0019-5189

issn

0975-1009

journal_volume

39

pub_type

杂志文章,评审
  • Infrared spectroscopic analysis of tumor pathology.

    abstract::Infrared spectra of normal and malignant breast tissues were measured in the 600 cm(-1) to 4000 cm(-1) region. The measured spectroscopic features which are the spectroscopic fingerprints of the tissues contain the vital information about the malignant and normal tissues. Fourier Transform Infrared (FTIR) data on 25 c...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Mehrotra R,Gupta A,Kaushik A,Prakash N,Kandpal H

    更新日期:2007-01-01 00:00:00

  • Isolation and identification of poly beta hydroxybutyric acid accumulating bacteria of Staphylococcal sp. and characterization of biodegradable polyester.

    abstract::Staphylococcus sp. strain BP/SU1, capable of degrading the biopolymer and utilize it as a source of carbon and energy, was isolated from activated sludge using METABOLIX (MBX D411G). It was found that this strain was capable of accumulating poly(3-hydroxybutyric acid) P(3-HB), as granule poly (3-hydroxybutyric acid), ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Roy B,Banerjee R,Chatterjee S

    更新日期:2009-04-01 00:00:00

  • Anti-oxidative protection against iron overload-induced liver damage in mice by Cajanus cajan (L.) Millsp. leaf extract.

    abstract::In view of the contribution of iron deposition in the oxidative pathologic process of liver disease, the potential of 70% methanolic extract of C. cajan leaf (CLME) towards antioxidative protection against iron-overload-induced liver damage in mice has been investigated. DPPH radical scavenging and protection of Fento...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sarkar R,Hazra B,Mandal N

    更新日期:2013-02-01 00:00:00

  • Anticonvulsant and behavioral actions of triterpene isolated from Rubia cordifolia Linn.

    abstract::Effect of a triterpene isolated from the acetone soluble part of petroleum ether extract of R. cordifolia was studied on convulsions induced by maximum electro shock (MES), electrical kindling and various chemoconvulsants in rats and mice. The effect of triterpene was also investigated on behavior and gamma-aminobutyr...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Kasture VS,Deshmukh VK,Chopde CT

    更新日期:2000-07-01 00:00:00

  • Aqueous extract of Ficus religiosa linn. reduces oxidative stress in experimentally induced type 2 diabetic rats.

    abstract::One of the major etiologies in pathogenesis of type 2 diabetes especially complications is oxidative stress. Aqueous extract of Ficus religiosa at a dose of 100 and 200 mg/kg orally decreased the fasting blood glucose in streptozotocin induced type 2 diabetic rats. The drug had enzyme induction effect with respect to ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Kirana H,Agrawal SS,Srinivasan BP

    更新日期:2009-10-01 00:00:00

  • Antisnake venom activity of ethanolic seed extract of Strychnos nux vomica Linn.

    abstract::The whole seed extract of S. nux vomica (in low doses) effectively neutralized Daboia russelii venom induced lethal, haemorrhage, defibrinogenating, PLA2 enzyme activity and Naja kaouthia venom induced lethal, cardiotoxic, neurotoxic, PLA2 enzyme activity. The seed extract potentiated polyvalent snake venom antiserum ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Chatterjee I,Chakravarty AK,Gomes A

    更新日期:2004-05-01 00:00:00

  • Role of antioxidant enzyme defence in sparing rat hepatocytes from toxicity of ricin at low dose.

    abstract::Ricin, a glycoprotein from castor oil seeds, is specifically toxic to Kupffer cells and at low doses it leaves parenchymal cells comparatively unaffected. At a dose of approximately 1.5 microgram/100 g body weight, ricin significantly increases the hepatic antioxidant enzyme system in rats within 24 hr. Superoxide dis...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sadani G,Nadkarni GD

    更新日期:1994-05-01 00:00:00

  • Molluscicidal activity of Piper cubeba Linn., Piper longum Linn. and Tribulus terrestris Linn. and their combinations against snail Indoplanorbis exustus Desh.

    abstract::The toxic effect of dried berries powder of P. cubeba, dried fruit powder of P. longum and T. terrestris singly as well as in combination [binary (1:1) and tertiary (1:1:1)] were studied against snail L. exustus. Toxicity of these plant products were time and concentration dependent. Ethanol extracts of these plants w...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Pandey JK,Singh DK

    更新日期:2009-08-01 00:00:00

  • Effect of silymarin on curcumin-induced mortality in zebrafish (Danio rerio) embryos and larvae.

    abstract::In presence of 7.5 microM of curcumin, no embryos or larva of zebrafish survived 3 days of incubation; however, coincubation with 144 microg/ml silymarin increased the survival rates of curcumin-treated embryos and larvae to about 70%. Moreover, in presence of 12.5 microM curcumin, all embryos died after 2 days of inc...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Shiau RJ,Shih PC,Wen YD

    更新日期:2011-07-01 00:00:00

  • Polyamines and plant alkaloids.

    abstract::Naturally occurring alkaloids are nitrogenous compounds that constitute the pharmacogenically active basic principles of flowering plants. Alkaloids are classified into several biogenically related groups. Tobacco alkaloids are metabolised from polyamines and diamines putrescine and cadaverine. N-methyl transferase is...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章,评审

    doi:

    authors: Ghosh B

    更新日期:2000-11-01 00:00:00

  • Effect of caspase inhibitors on the post-thaw motility, and integrity of acrosome and plasma membrane of cryopreserved equine spermatozoa.

    abstract::The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spe...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Peter AT,Colenbrander B,Gadella BM

    更新日期:2005-06-01 00:00:00

  • Binding pattern of 125iodine thyroxine and tri-iodothyronine in skin and liver tissues of spotted munia, Lonchura punctulata: co-relation to seasonal cycles of breeding and molting.

    abstract::Prevalent notion about thyroid hormones is that thyroxine (T4) is a mere precursor and physiological effects of thyroid hormones are elicited by tri-iodothyronine (T3) after mono-deiodination of T4. Earlier studies on feather regeneration and molt done on spotted munia L. punctulata suggest that T4 (mono-deiodination ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Thapliyal A,Chandola-Saklani A,Bhatt D,Anthwal P

    更新日期:2014-05-01 00:00:00

  • Effect of dietary fiber from banana (Musa paradisiaca) on metabolism of carbohydrates in rats fed cholesterol free diet.

    abstract::Effect of feeding isolated dietary fiber from M. paradisiaca on the metabolism of carbohydrates in the liver has been studied. Fiber fed rats showed significantly lower levels of fasting blood glucose and higher concentration of liver glycogen. Activity of glycogen phosphorylase, glucose-1-phosphate, uridyl transferas...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Usha V,Vijayammal PL,Kurup PA

    更新日期:1989-05-01 00:00:00

  • The lld mutation in Pisum sativum used as a genetic tool to discern the plant leaflet/leaf developmental process.

    abstract::Leaves of P. sativum the double mutant genotype tendril-less (tl) leaflet-development (lld), due to the action of lld mutation, produce many leaflets that are aborted at different stages of development. Morphological, vein pattern and histological observations showed that aborted leaflets became cup/bell/trumpet (cup)...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sharma V,Tripathi BN,Kumar S

    更新日期:2013-06-01 00:00:00

  • Polypeptide pattern of mRNA isolated from polysomal and non-polysomal mRNA-protein(mRNP) fractions in rat myoblast.

    abstract::Polypeptides synthesized from polysome-bound and unbound fractions of polyA mRNA were studied by in-vivo cells labelling and cDNA-mRNA hybrid-arrest translation. The polypeptides synthesized from unbound(free) fraction appeared to be significantly reduced in the absence of cycloheximide in L6 myoblast. The cDNA excess...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Bhattacharya P

    更新日期:1997-09-01 00:00:00

  • Radioiodide uptake and sodium iodide symporter expression in breast carcinoma.

    abstract::Breast cancer is a common malignancy in women all over the world and novel therapeutic approaches are required for the treatment of patients who become refractory to conventional therapies. Thyroid cancer is being treated successfully with radioiodine since many years. The iodide is transported inside the thyroid epit...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Damle AA,Narkar AA,Badwe RA

    更新日期:2011-06-01 00:00:00

  • Dioxygen reduction, reduced oxygen species, oxygen toxicity and antioxidants — A commentary.

    abstract::Molecular oxygen, a diradical, needs intervention of redox metal ions or other radicals to receive electrons for its reduction. The oxygen radicals thus produced are responsible for oxygen toxicity and oxidative stress. But, autoxidation, relevant in ischemia-reperfusion injury, is absent in any discussion on oxygen t...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ramasarma T

    更新日期:2016-11-01 00:00:00

  • Effect of undernutrition on succinate dehydrogenase and acetylcholinesterase in developing rat brain.

    abstract::Pups (5 days old) were undernourished by separating them for 14 hr daily from their mothers for 7, 10, 13, 16 and 20 days. The undernourished rats showed significant decrease in body and brain weight, protein and nucleic acid contents at all stages of observation as compared to controls. The activities of SDH and AChE...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Singh UK,Agarwal KN,Shanker R

    更新日期:1990-09-01 00:00:00

  • Circadian features of carbohydrate metabolism in domestic fowls exposed to weekly 120 degree-shifts of synchronizer schedule.

    abstract::Effects of weekly 8 hr advance- or delay-shifts on the circadian rhythm of plasma glucose, liver glycogen and muscle glycogen in male domestic fowls, beginning at about 3 days of age, were examined. Circadian rhythm in the aforesaid indices of carbohydrate metabolism in control birds was also studied. Blood and tissue...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Saini SK,Pati AK

    更新日期:1992-02-01 00:00:00

  • Potential application of beta-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction.

    abstract::Pseudomonas aeruginosa MCCB 123 was grown in a synthetic medium for beta-1,3 glucanase production. From the culture filtrate, beta-1,3 glucanase was purified with a molecular mass of 45 kDa. The enzyme was a metallozyme as its beta-1,3 glucanase activity got inhibited by the metal chelator EDTA. Optimum pH and tempera...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Jose D,Jayesh P,Gopinath P,Mohandas A,Singh IS

    更新日期:2014-01-01 00:00:00

  • Biophotons from stressed and dying organisms: toxicological aspects.

    abstract::Cells and organisms exposed to detrimental and toxic substances show different responses in photon emission dependent on amount, kind and exposure time of toxin as well as on the organism investigated. Radical reaction-generating substances and dehydrating, lipid dissolving and protein denaturating toxins which do not...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sławinski J

    更新日期:2003-05-01 00:00:00

  • Investigations on wound healing by using amphibian skin.

    abstract::The present investigation is aimed to justify the validity of traditional practice of using the frog skin in wound healing. Studies were carried out on skin wounds from female albino rats of Wistar strain weighing 100-120 g. The wounds of standard size (5 cm2) were created in the experimental and control groups. The e...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Sai KP,Reddy PN,Babu M

    更新日期:1995-09-01 00:00:00

  • Antioxidant DL-alpha lipoic acid as an attenuator of adriamycin induced hepatotoxicity in rat model.

    abstract::Protective efficacy of DL-alpha lipoic acid on adriamycin induced hepatotoxicity was evaluated in rats. Adriamycin toxicity, induced by a single injection (ip; 15 mg/kg body wt), was expressed by an elevation in alanine transaminase, aspartate transaminase, bilirubin levels in serum and alkaline phosphatase, lactate d...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Anandakumar PP,Malarkodi SP,Sivaprasad TR,Saravanan GD

    更新日期:2007-12-01 00:00:00

  • Evaluation of Mycobacterium tuberculosis specific RD antigens for delayed type hypersensitivity responses in guinea pig.

    abstract::Tuberculin skin test (TST), an age old method is based on measuring delayed-type hypersensitivity (DTH) response to purified protein derivative (PPD). However, inspite of simplicity, ease and cost effectiveness, the usefulness of PPD test is limited due to its inability to distinguish among a protective immune respons...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Kalra M,Khuller GK,Sheikh JA,Verma I

    更新日期:2010-02-01 00:00:00

  • Effect of sodium nitroprusside on H+-ATPase activity and ATP concentration in Candida albicans.

    abstract::ATP hydrolysis by plasma membrane H+-ATPase from Candida albicans has been investigated in presence of nitric oxide and various nutrients (sugars and amino acids). Sodium nitroprusside (SNP) was used as nitric oxide donor. It was found that ATP concentration decreased in SNP treated cells which was more in presence of...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Haque MM,Pooja,Manzoor N,Khan LA,Basir SF

    更新日期:2005-10-01 00:00:00

  • Wheat (Triticum aestivum) peptide (s) mimic gibberellin action and regulate stomatal opening.

    abstract::Wheat peptides (0.5 to 3 KDa M(r)) mimick hormonal activity like that of gibberellins and forced open dark closed stomata. The deionized amphoteric peptides solution after passing through cation and anion exchanger resins was run through Amicon's ultrafilters, 10, 3 and 0.5 kDa (M(r)) cut off system. The 3 to 0.5 kDa ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Ghosh A,Mandal P,Sircar PK

    更新日期:2010-01-01 00:00:00

  • Anti-fungal potentials of extracellular metabolites of Western Ghats isolated Streptomyces sp. NII 1006 against moulds and yeasts.

    abstract::Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Jayamurthy H,Valappil Sajna K,Dastagar SG,Pandey A

    更新日期:2014-11-01 00:00:00

  • Antiparkinsonian activity and dopamine receptor binding studies of imidazolone derivatives.

    abstract::Mannich bases and styryl derivatives of imidazolones were evaluated for their antiparkinsonian activity. Two compounds showed potent antiparkinsonian activity. These active compounds also showed binding with dopamine receptors in striatal membrane preparation of rat brain. ...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Naithani PK,Srivastava VK,Saxena AK,Barthwal JP,Gupta TK,Shanker K

    更新日期:1990-12-01 00:00:00

  • Influence of gonadotropins on adrenalectomy induced changes in the testis of albino mouse.

    abstract::Effects of adrenalectomy and administration of gonadotropins on cell counts of different cell types of spermatogenesis and morphology of the Leydig cells were studied in 30 day old mice. Adrenalectomy (duration, 12 days; age at autopsy 42 days) caused a significant decrease in the diameters of seminiferous tubules and...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Bhagya M,Yajurvedi HN

    更新日期:1999-02-01 00:00:00

  • Impact of feeding ethanolic extract of root bark of Cananga odorata (Lam) on reproductive functions in male rats.

    abstract::The 50% ethanolic extract of the root bark of C. odorata administered orally at the dose of 1g/kg body weight/day for 60 days resulted in decreased epididymal sperm motility and sperm count in male albino rats. Morphological abnormalities were also observed in the sperms. The testicular glycogen, the activities of 3be...

    journal_title:Indian journal of experimental biology

    pub_type: 杂志文章

    doi:

    authors: Anitha P,Indira M

    更新日期:2006-12-01 00:00:00