Abstract：
:Frog plasma effectively hydrolysed the synthetic chromogenic substrates, H-D-Glu-Gly-Arg-p-nitroanilide (S-2444), benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide (S-2222) and acetyl-Ile-Glu-Gly-Arg- p-nitroanilide (S-2423), all sensitive substrates for trypsin. Moderate hydrolytic activities was observed with H-D-Phe-Pip-Arg-p-nitroanilide (S-2238, substrate for thrombin) and H-D-Pro-Phe-Arg-p-nitroanilide (S-2302, substrate for plasma kallikrein). Frog plasma contained moderate alpha-macroglobulin activity. When plasma was incubated at 37 degrees C, the macroglobulin activity decreased in a time dependent manner while only a moderate decrease in the protease activity was observed. Ten fold dilution of plasma with 0.1 M phosphate buffer, pH 7.6 prevented the inherent loss of macroglobulin activity but it had no effect on protease activity. Dye ligand chromatography of the plasma on red Sepharose revealed that bulk of alpha-macroglobulin activity along with minor proteolytic activity (S-2222 hydrolysis) was present i the washings. On the other hand, about one third of the alpha-macroglobulin activity and bulk of the protease activity was bound to the column and were eluted with 1.5 M NaCl. alpha-Macroglobulin activity in red Sepharose washings and elutions on chromatography on Sephadex G-200, was eluted in two regions with Ve/Vo value of 1.33 and 1.08, respectively.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Kumar RSsubject
Has Abstractpub_date
1997-11-01 00:00:00pages
1198-202issue
11eissn
0019-5189issn
0975-1009journal_volume
35pub_type
杂志文章abstract：:Incidence of obesity and diabetes is increasing at an alarming rate not only among the populations of the affluent nations but also amongst the populations of the developing nations. Understanding the mechanisms that cause the onset of these pathological conditions is a requisite to effectively tackling this problem. ...
journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
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doi：
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doi：
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
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