Abstract：
:Melanoma antigen-encoding gene 3 (MAGE-3) is an ideal candidate for a tumor vaccine although its potency need to be increased. Heat shock proteins (HSPs) represents a potential approach for increasing the potency of DNA vaccines. In the present study, a fusion DNA vaccine composed of Mycobacterium tuberculosis HSP70 and MAGE-3 was constructed and used to immunize C57BL/6 mice against B16 or B16-MAGE-3 tumor cells. The results show that the HSP70-MAGE-3 fusion DNA vaccine enhanced the frequency of MAGE-3-specific cytotoxic T-cells as compared to the MAGE-3 DNA vaccine or the HSP70/MAGE-3 cocktail DNA vaccine (P < 0.05). In conclusion, the results indicate that the HSP70-MAGE-3 fusion DNA vaccine can strongly activate MAGE-3 specific cellular immunological reactions and thus significantly inhibit the growth of B16-MAGE-3 tumors, improving the survival of tumor-bearing mice, and the HSP70-MAGE-3 fusion DNA vaccine has a significant therapeutic effect on the tumors that express MAGE-3 antigens.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Qu P,Ma JH,Zhang XM,Huang XJ,Yang XW,Yan-Fang Ssubject
Has Abstractpub_date
2010-05-01 00:00:00pages
436-43issue
5eissn
0019-5189issn
0975-1009journal_volume
48pub_type
杂志文章abstract：:Three lipophilic amide derivatives of phthaloyl-GABA (P-GABA), namely gamma-phthalimido N-amyl butyramide (PGA), gamma-phthalimido-N-hexylbutyramide (PGH) and gamma-phthalimido N-phenylbutyramide (PGP), were synthesized and evaluated for their hypnotic and anticonvulsant activities in mice. Both PGA and PGH showed mod...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:Supplementation of cyanate in rats caused a significant decrease in serum GSH and increase in calcium and phosphate level both in serum and lens. Consequently, these changes led to induce acidosis uremia in serum and hypercalcemia and hyperphosphatemia in lens which may be possible causing factor for cataract. ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The effects of A. indica (AI, Neem) were evaluated on tests of humoral and cell-mediated immune responses after 3 weeks of oral AI (leaf extract) treatment in ovalbumin immunized mice. At the dose levels tested, AI (10, 30 or 100 mg/kg), had no appreciable influence on different organ (liver, spleen, thymus)/body weig...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:In an attempt to evaluate the effect and interaction of ethanol on endosulfan-induced hepatotoxicity in vivo to adult male rats, both, endosulfan (7.5 mg/kg body wt) and ethanol (1.5 g/kg body wt) were studied separately as well as in combination after a chronic oral exposure of 30 days. When fed separately, both the ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:Chemical composition of inter and intracellular hyphae was elucidated in Pennisetum pedicellatum roots colonized by Gigaspora margarita by employing energy dispersive X-ray microanalysis. Maximum amount of phosphate was estimated in polyphosphate granules present in the roots. Amount of phosphate was low in fungal hyp...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:The implantation failure in newly inseminated mice induced by food deprivation for 48 hr, beginning at 0900 hrs on day 4 post coitum, was prevented by simultaneous exposure to light continuously for 48 or 36 hr. Food-deprived females that were exposed to continuous light for 36 hr showed a significant increase in feta...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Skin and tendon samples of male albino rat taken for analysis, on the 120th day of smoking showed that, compared to controls, the cigarette smoke exposed group showed an increase in tensile strength of both skin and tendon while extensibility of skin remained the same and that of the tendon increased. Thermal behaviou...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10 +/- 0.55 with an average of 19.93 +/- 3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
abstract：:Creatinine in serum or urine is determined by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm. Without taking into consideration the acidi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Adrenal delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activity and serum corticosterone level were significantly higher in rats fed with 5% casein or 4% albumin diets after 1 hr of ether anaesthetic stress as compared to the controls, 5% casein and 20% casein (equivalent to 4% albumin) respectively. Eth...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:The effect of long term administration of thyroid hormones and its deprivation on delayed type hypersensitivity (DTH) reaction to 2-4 dinitrochlorobenzene (DNCB) was studied. Animals were either pre-treated with thyroid hormones (T3 or T4) for 15 days and then subjected to DNCB skin test or the animals received thyroi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:Rate of heat production during cell proliferation following UV-irradiation of respiratory-deficient yeast cells was measured as a function of time (p-t curve) in a batch microcalorimeter. Following observations were made: (a) All growing cell cultures showed 3 distinct phases of heat production namely lag, exponential...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:The xanthine oxidoreductase (XOR) system which consists of xanthine dehydrogenase (XDH) and xathine oxidase (XO), is one of the major sources of free radicals in biological systems. The XOR system is pre-dominantly present as XDH in normal tissues and converts into the free radical generating XO-form in the damaged ti...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2003-02-01 00:00:00
abstract：:Cyclophosphamide is a potent immunosuppressive agent and is being widely used in organ transplantation. The effects of this anti-rejection drug on lymphoid organs are poorly understood. Newborn Swiss mice injected with various doses of cyclophosphamide suffered from wasting disease at 4 weeks post treatment. The incid...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:In the present investigation, growth of the organisms was reduced due to presence of arsenic (III) and (V) in the culture medium. In comparison to arsenic (V), arsenic (III) had more toxic effect on microalgae. Among the different algal strains, blue green algal species Oscillatoria-Lyngbya mixed culture showed maximu...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:Enzyme cyclooxygenase (COX) is reported to play a significant role in neurodegeneration and may play a significant role in the pathogenesis of epilepsy. Bicuculline (4 mg/kg; ip), picrotoxin (8 mg/kg; ip) and electroshock (60 mA for 0.2 sec) significantly induced convulsions in male Laka mice. COX-inhibitors viz. nime...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:Four plants having known medicinal properties were screened for inhibition of goatpox virus (GTPV) replication in vitro. Of the 4 plants, extract of Acacia arabica (Babul) and Eugenia jambolana (Jamun) leaves had inhibition (%) 99.70 and 99.92 at their maximum non toxic concentrations, 99.93 +/- 0.38 and 1999.73 +/- 0...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-02-01 00:00:00
abstract：:Effects of tamoxifen (Tam) on cytosolic estradiol (E2) receptors (ERc), progesterone (P4) receptors (PRc), nuclear estradiol (ERn) and progesterone receptors (PRn) were studied in adult normal rabbit uterine tissue. The ratio of cytosol: nuclear estradiol receptors (ER) was greater in rabbits treated with Tam than E2 ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:Orchidectomy caused a significant decrease in the concentration of ascorbic acid and ascorbic acid-2-sulphate in tissues while administration of testosterone to these animals reverses this effect. Concentration of ascorbic acid in liver in experimental groups correlates with the activity of L-qualano-lactone oxidase a...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:A cyclic colony of P. papatasi was successfully established, using wild caught females. The major obstacle in the colonization was infestation of fungus, which was solved when bentonite, a dehydrant was mixed in the larval diet i.e., powdered and sterilized faecal pellets of rabbit. The average duration of development...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:Present investigation was undertaken to evaluate antihyperglycemic, antihyperlipidemic and antioxidant activities of Dihar, a polyherbal formulation containing drugs from eight different herbs viz., Syzygium cumini, Momordica charantia, Emblica officinalis, Gymnema sylvestre, Enicostemma littorale, Azadirachta indica,...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：:Lead (100 ppm) was given in doubly deionised water for 30 days to one group of rats. The other groups received lead along with exogenous antioxidants like vitamin E (50 IU/kg), vitamin C (800 mg/kg) or Spirulina (1500 mg/kg) in food for a similar period. Levels of lipid peroxidation products such as malondialdehyde, c...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The effect of exogenous administration of cortisol (0.2 microg/g body weight) for 24, 48 and 72 hr on the gill epithelium of Tilapia has been studied. The results clearly revealed that out of the three sub-types of chloride cells viz., shallow basin, wavy convex and deep hole, the shallow basin ones are the most abund...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-04-01 00:00:00
abstract：:The possibility of stimulation of C-reactive protein (CRP) due to inflammation and necrosis caused by cadmium was investigated. Cadmium injection (less than 3 mg/kg body weight) in white rat was found to be associated with the appearance of CRP in liver cytosol after 8 hr and in serum after 18 hr of injection. Althoug...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:Mowrah (M. latifolia) seeds yield 40-50% edible fat and the meal contains saponins besides protein and high level of carbohydrates. The toxicity of the meal was evaluated as it has a potential for use in animal feedstuffs. The meal was fed to young and adult rats at levels of 10 to 40% in diet. The animals showed mark...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Orange peel oil is used extensively as an approved flavour enhancer in fruit drinks, carbonated beverages and as a scenting agent in soaps and cosmetics. Limonene, which is a monocyclic monoterpene is present in orange peel oil from 90 to 95% (w/w). Monoterpenes have been shown to be very effective chemopreventive age...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:One of the major etiologies in pathogenesis of type 2 diabetes especially complications is oxidative stress. Aqueous extract of Ficus religiosa at a dose of 100 and 200 mg/kg orally decreased the fasting blood glucose in streptozotocin induced type 2 diabetic rats. The drug had enzyme induction effect with respect to ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-10-01 00:00:00
abstract：:The in vitro percutaneous absorption of verapamil hydrochloride (VHCl) was investigated in order to assess its feasibility for transdermal development. The experiments were carried across mice and guinea pig skins using Keshary-Chien diffusion cell. The values of diffusion rate (J) and permeability coefficient (Kp) ac...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:BC-8, a rat histiocytoma undergoes apoptosis after heat shock, which is due to lack of an effective heat shock response. Heat shock induced generation of free radicals, which in turn are involved in the induction of apoptotic death in BC-8 cells. Treatment of BC-8 cells with N-acetylcysteine partially inhibited the he...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-10-01 00:00:00
abstract：:The present investigation is aimed to justify the validity of traditional practice of using the frog skin in wound healing. Studies were carried out on skin wounds from female albino rats of Wistar strain weighing 100-120 g. The wounds of standard size (5 cm2) were created in the experimental and control groups. The e...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
