Abstract：
:Creatinine in serum or urine is determined by Jaffe's reaction where creatinine produces quantitatively an orange color with picric acid in alkaline medium. After allowing an incubation time of 15 min at room temperature for color development the color is measured at 520 nm. Without taking into consideration the acidic nature of standard, protein free filtrate (PFF) of serum and urine, 1% picric acid and 0.75N NaOH are used in this reaction for color development in standard, PFF of serum and urine. An investigation was thought to be necessary to determine the optimum alkali concentration required in standard, PFF of serum and urine. The results show that 0.25, 0.75 and 1N NaOH give maximum color in urine, standard and PFF of serum respectively. A standard solution of creatinine is prepared in 0.1N HCl and the PFF of serum is obtained by addition of fresh tungstic acid. Alkali is consumed to neutralise the acids in both these cases. For urine creatinine measurement, a direct diluted urine sample is used. The difference in the requirement of NaOH is conceivable. The routine use of 0.75N NaOH irrespective of the nature of specimen as is done in all biochemical laboratories, for creatinine measurement needs modification in the light of this investigation.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Toora BD,Rajagopal Gkeywords:
subject
Has Abstractpub_date
2002-03-01 00:00:00pages
352-4issue
3eissn
0019-5189issn
0975-1009journal_volume
40pub_type
杂志文章abstract：:Infectious pancreatic necrosis virus (IPNV) from lake trout of Cornwall lake (LT-IPNV), from trout of Jasper (Ja-IPNV) and Arctic char of Northwest Territories (AC-IPNV) are the three isolates recorded from western Canada. Two segments (nt 453-674 and nt 1197-1503) from VP2 coding region of RNA of the isolates were am...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:Adrenal delta5-3beta-hydroxysteroid dehydrogenase (delta5-3beta-HSD) activity and serum corticosterone level were significantly higher in rats fed with 5% casein or 4% albumin diets after 1 hr of ether anaesthetic stress as compared to the controls, 5% casein and 20% casein (equivalent to 4% albumin) respectively. Eth...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1996-10-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
更新日期：2001-11-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2003-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2016-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Studies presented in this paper examined the tumor-specific cellular and humoral immunity induced by anti-idiotype antibodies (Ab2s) 2F10 and 3A4. A panel of Ab2s was made against a monoclonal anti-L1210/GZL lymphoma, 11C1. The Ab2s were screened for their ability to block 11C1 binding to tumor, to induce tumor-specif...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1989-08-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2005-08-01 00:00:00
abstract：:Photons participate in many atomic and molecular interactions and processes. Recent biophysical research has discovered an ultraweak radiation in biological tissues. It is now recognized that plants, animal and human cells emit this very weak biophotonic emission which can be readily measured with a sensitive photomul...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
