Abstract：
:Various storage treatments on human blood samples have been described with respect to DNA yield, quality and fingerprinting. Blood samples were stored at room temperature, 4 degrees C, -20 degrees C and -70 degrees C for different duration varying from 1, 2, 4 and 8 weeks with or without anticoagulant/preservative (EDTA or Heparin). DNA was isolated from these stored samples, quantitated by spectrophotometry and subjected to DNA fingerprinting using a human alphoid satellite DNA sequence (TRF 5.6) and a 33 mer oligonucleotide (O-chi-1) as probes. The polymorphic DNA bands were apparent between 2 to 9 kb size range and the fingerprints were individual-specific. Our results suggest that higher amount of genomic DNA can be recovered from blood samples stored at temperatures 4 degrees C or below in the presence of EDTA or heparin.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Bomjen G,Raina A,Sulaiman IM,Hasnain SE,Dogra TDsubject
Has Abstractpub_date
1996-04-01 00:00:00pages
384-6issue
4eissn
0019-5189issn
0975-1009journal_volume
34pub_type
杂志文章abstract：:The investigations on enzymes related to glutathione like glutathione-S-transferase (GST) and glutathione peroxidase (GSH-Px) have been carried out mostly in human and rat ovaries, however the studies on these enzymes in ruminants are relatively absent. In the present study the changes in the activity of these enzymes...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:The toxic effect of dried berries powder of P. cubeba, dried fruit powder of P. longum and T. terrestris singly as well as in combination [binary (1:1) and tertiary (1:1:1)] were studied against snail L. exustus. Toxicity of these plant products were time and concentration dependent. Ethanol extracts of these plants w...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：:The crude buffer (Tris Buffer Saline-I) extracts of muscles, liver, kidney and heart of goat and chicken (White leghorn) were screened against 16 clinical isolates. Among the five tissues, the heart tissue of each animal showed significant bactericidal activities on many isolates. The acid extracted crude proteins of ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:Lipopolysaccharide (LPS) and ratio-detoxified LPS (Rd-LPS) from Salmonella typhimurium were analysed for their ability to stimulate murine peritoneal exudate cells (PEC) and macrophages. Rd-LPS induced much more inflammatory response as compared to LPS. PEC numbers/mouse obtained were significantly higher (3-fold) in ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:This review is an attempt to comprehend the diverse groups of environmental chemical contaminants with a potential for pathogenesis of breast cancer, their probable sources and the possible mechanisms by which these environmental contaminants act and interplay with other risk factors. Estrogens are closely related to ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2006-08-01 00:00:00
abstract：:Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:To investigate the acute effect of ethanol (4 g/kg, i.p.) on spleen adult female Wistar rats were treated intraperitoneally with: a) ethanol (4 g/kg body wt), b) naltrexone (5 mg/kg body wt) followed 45 minutes later by ethanol (4 g/kg body wt) and c) naltrexone (5 mg/kg body wt) alone. Untreated and saline-treated ra...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:Xenobiotics have played a role in elucidating the regulation of gene expression of hepatic cytochrome P450 in the eukaryotes. The major regulation of P450 genes in the eukaryotes is at the transcriptional and post transcriptional level. Polycyclic aromatic hydrocarbons regulate the gene expression by binding the cytos...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1998-05-01 00:00:00
abstract：:Staphylococcus sp. strain BP/SU1, capable of degrading the biopolymer and utilize it as a source of carbon and energy, was isolated from activated sludge using METABOLIX (MBX D411G). It was found that this strain was capable of accumulating poly(3-hydroxybutyric acid) P(3-HB), as granule poly (3-hydroxybutyric acid), ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-04-01 00:00:00
abstract：:Dose matched comparison between copper complex of tolmetin(Tol-Cu) and tolmetin(Tol) was carried out in male albino rats bearing either sutured incision, dead space or excision wounds. Results showed that Tol significantly decreased tesile strength of incision and dead space wound. This effect was totally reversed by ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:Shoot tip explants of 5.chickpea varieties were evaluated for efficiency of rooting using MS basal medium (1/4, 1/2 and full strength). Maximum rooting was observed in 3 varieties when cultured in MS medium with 1/4 strength. Plantlets thus developed, were further cultured in Hoagland solution (1/4 strength) for 15-20...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Nine halophilic archaea viz., Halobacterium salinarum, Halobacterium sp.1, Halobacterium sp.2, Halobaculum sp., Halococcus saccharolyticus, Halorubrum saccharovorum, Haloterrigena turkmenica, Halogeometricum sp. and Natrialba sp. isolated from marine salterns around Bhavnagar coast were screened for siderophore produc...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:On the basis of inhibition analysis, tyrosine uptake in mouse mammary gland was found to be mediated by Na(+)-dependent and Na(+)-independent systems. Na(+)-dependent system was insensitive to 2-(methylamino) isobutyric acid (MeAIB), with an apparent Km of 1.67 mM and maximal velocity 74.5 nmol.g-1 cell. min-1. Compet...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Seven L. acidophilus strains were examined for their antibiotic sensitivity against various chemotherapeutic agents and all of them were found to be sensitive to novobicin, chloramphenicol, ampicillin, erythromycin, oxytetracycline and chlorotetracycline, whereas, all were resistant to norfloxacin and nalidixic acid. ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:Dicarboxylic acids and a few amino acids were found to support mitochondrial phosphorylation in A. ceylanicum. Anaerobiasis markedly reduced this activity. Maximum effect was observed on succinate supported phosphorylation which in anaerobic atmosphere yielded only 2% ATP compared to that in the presence of air. Known...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:Effects of ampicillin (Amp) in combination with riboflavin on septic arthritis in mice infected with Staphylococcus aureus have been reported. Ampicillin was given at 100 mg/kg after 24 h of infection, followed by riboflavin (Ribo) at 20 mg/kg body wt, after 2 h of Amp treatment. Mice were sacrificed at 3, 9, 15 days ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：:The present study was undertaken to identify the receptor for dengue virus type 2 (DV) induced macrophage cytotoxin (CF2) on mouse peritoneal macrophages (MPhi). The binding of 125I-labelled CF2 to MPhi was saturable (15 nM), reversible, temperature, pH- and time-dependent. The saturation concentration was similar to ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:The p38 mitogen activated protein kinase (p38MAPK) pathway is an important signaling cascade involved in cell growth, differentiation and apoptosis. High glucose activates p38MAPK pathway in different cells, including osteoblasts. In the present study, role of p38MAPK in high glucose induced osteoblast apoptosis and p...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-02-01 00:00:00
abstract：:Blood samples of malaria patients (n = 30), selected based on the severity of parasitemia, were divided into low (LP), medium (MP) and high (HP) parasitemia, which represent increasing levels of the disease severity. Healthy subjects (n = 10) without any history of disease were selected as a control group. By processi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:Effects of 1,1,1,-trichloro-2,2-bis (p-chlorophenyl) ethane (DDT) and lindane were studied on gamma glutamyl transpeptidase (GGT) activity in different tissues of the lymphoid system in rats. DDT (100 or 200 ppm) and lindane (30 or 80 ppm) exposure for 8 weeks suppressed the GGT activity in a dose dependent manner in ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:ATP hydrolysis by plasma membrane H+-ATPase from Candida albicans has been investigated in presence of nitric oxide and various nutrients (sugars and amino acids). Sodium nitroprusside (SNP) was used as nitric oxide donor. It was found that ATP concentration decreased in SNP treated cells which was more in presence of...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
abstract：:Ganoderma lucidum, a white rot fungus, was exploited for its potentials to produce xylanase employing shake and solid-state culture conditions. Different culture conditions such as pH, temperature, carbon and nitrogen requirements for its growth and production of xylanase were optimized. The culture media pH 6.0-7.0 a...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:When compared with the standard free muscle grafts, predenervated and nerve crushed chick gastrocnemius muscle grafts followed an identical and ordered sequence of tissue degeneration-regeneration. Atrophy of the fibres during ischaemic degeneration was accompanied by loss of weight, noncollagenous proteins and increm...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Understanding the biochemical and molecular basis of drought mechanism in rice is important as drought is one of the major causes affecting rice crop adversely. A 1017 bp gene sequence encoding AP2/ERF family TF was isolated from Oryza sativa sp. Indica cv N22 encoding a protein of 338 amino acid residues, with a mole...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：:Efficacy of two colorimetric assays, viz. MTT (3-4,5-dimethylthiazol-2-(yl-2,5-diphenyl tetrazolium bromide) and neutral red (NR) assays, performed by integrating them to micro culture virus titration (MCVT), was compared with the conventional MCVT method in terms of percentages of infectivity and 50% infectivity end ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Two wheat varieties, T. durum (HD 4502) and T. aestivum (Kalyansona) were examined for photosynthesis rate and contents of sugars and rubisco protein in the flag leaf, at forenoon and afternoon at anthesis stage. A decrease in photosynthesis rate was observed in the afternoon compared to forenoon in both the varieties...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:A new gamma-aminobutyric acid derivative, N-phthaloyl GABA (P-GABA), was synthesised and its anticonvulsant activity was tested and compared with sodium valproate for efficacy against experimentally induced convulsions in mice. At a dose of 80 mg/kg, P-GABA rendered more protection than sodium valproate. ED50 of P-GAB...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Effect of cerebellar lesion and vestibular stimulation (VS) on the activity and alternation of ECL-cells along with changes in gastric volume and acid secretion was studied. The results suggest that cerebellar lesion caused increased gastric volume and acid secretion and tended to decrease ECL-cell density. On the oth...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:A bioactive compound with antibacterial activity was isolated and purified from the extract of leaves of sunflower, Helianthus annuus. The bioactive compound was characterized using 1H and 13C NMR. The compound induced auxin, gibberellins and cytokinin in Oryza sativa and Phaseolus mungo. It also showed activity again...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:Antinociceptive activity of methanolic extract of leaves of A. aspera was studied by peripheral/non-narcotic model of nociception like acetic acid induced writhing syndrome test and central/narcotic models like hot plate and tail flick tests. The methanolic extract of the plant, administered orally (@ 300, 600 and 900...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2010-08-01 00:00:00
