Abstract:
:ATP hydrolysis by plasma membrane H+-ATPase from Candida albicans has been investigated in presence of nitric oxide and various nutrients (sugars and amino acids). Sodium nitroprusside (SNP) was used as nitric oxide donor. It was found that ATP concentration decreased in SNP treated cells which was more in presence of sugars like glucose, xylose and 2-deoxy-D-glucose and amino acids as compared to their respective controls. The activity of H+-ATPase from plasma membrane decreased by 70 % in SNP treated cells. Both in vivo and in vitro treatments of SNP showed almost similar effects of decrease in ATPase activity. Effect of SNP was more pronounced in presence of nutrients. Interestingly, it was observed that vanadate did not show any independent effect in presence of nitric oxide. Several workers have reported similar type of results with other P-type ATPases. For the first time, it was observed in the present study that in presence of nitric oxide, H+-ATPase activity decreased like other P-type ATPases. Our study indicated that NO had a significant effect on ATP synthesis and activity of H+- ATPase. In the presence of NO, the ATP concentration was decreased indicating it affected mitochondrial electron transport chain. It may be concluded that NO, not only affects (inhibit) mitochondrial electron transport chain but also interferes with H+- ATPase of plasma membrane by changing its conformation resulting in decreased activity.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Haque MM,Pooja,Manzoor N,Khan LA,Basir SFkeywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
873-9issue
10eissn
0019-5189issn
0975-1009journal_volume
43pub_type
杂志文章abstract::Ethanol extracts from the different parts of B. orellana showed differential antimicrobial activity. It was found that the extracts of in vitro leaves showed maximum activity against Bacillus pumilus followed by the extracts from the roots and hypocotyls. The callus derived from different explants too showed antimicro...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::Ten oleaginous yeasts were isolated from sources like fruits, flowers, curd whey and soil samples. Lipid production varied from 24 to 71.2% of dry weight of cell biomass. Strain Y-1, which was isolated from curd whey, produced the highest amount of lipid. It was identified belonging to the genus Candida. The optimal c...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Role of fatty acid binding proteins (FABPs) in modulating inhibition of human placental malate dehydrogenase by palmitoyl-CoA and oleate has been studied. Activity of human placental cytosolic malate dehydrogenase is detected throughout the gestation, showing a peak at midgestation (20-25 weeks). Inhibition (50%) of t...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract::To study antimicrobial activity of shallot in comparison with that of garlic and onion against 23 strains of fungi and bacteria, water extracts of garlic, shallot and onion bulbs were prepared. Each extract was studied in different forms for their antimicrobial activity viz., fresh extract, dry extract and autoclaved ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::BC-8, a rat histiocytoma undergoes apoptosis after heat shock, which is due to lack of an effective heat shock response. Heat shock induced generation of free radicals, which in turn are involved in the induction of apoptotic death in BC-8 cells. Treatment of BC-8 cells with N-acetylcysteine partially inhibited the he...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::Nicotine administration (2.5 mg/kg of body weight, sc, 5 days a week for 22 weeks) enhanced lipid peroxidative indices (thiobarbituric acid reactive substances and hydroperoxides) accompanied by a significant increase in the marker enzymes alanine transaminase, aspartate transaminase, alkaline phosphatase and lactate ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2007-02-01 00:00:00
abstract::EHb-a herbo-mineral formulations of iron (ferrous form) produced a significantly higher and dose dependent increase in the haemoglobin level, as compared to Fefol (a non-complex-chelated iron preparation). Also, EHb did not produce any overt toxicity or gastric irritation at these dose levels. The results suggest that...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2011-05-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Formation of strand-breaks in DNA and its repair in Yoshida ascites tumor cells exposed to gamma radiation (100-400 Gy) in presence and absence of misonidazole (10 mM) were studied. The methodology involved pre-labelling of cellular DNA by 3H-thymidine during cell proliferation in rats, irradiation of cells in vitro a...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::Biotin deficiency produced by feeding raw egg white caused decrease in the concentration of many glycosaminoglycans (GAG) in the aorta in rats fed standard laboratory diet and atherogenic diet. This decrease has been observed to be due to the increased activity of enzymes involved in the degradation of GAG and glycohy...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Anticancer potential of Moringa oleifera L. extracts have been well established. However, there are no reports on the isolated molecules/fractions from these extracts which are responsible for the anticancer/cytotoxic activity. Thus, in the present study, we explored the same. The n-hexane, chloroform, ethyl acetate, ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2015-02-01 00:00:00
abstract::Shoots of apple rootstocks raised in vitro were transferred to various rooting media to study the effect of different factors on root initiation and development. Various concentrations of indole-3-butyric acid (IBA) initiated rooting but maximum rooting percentage was found with 2.0 and 2.5 mg l(-1) of IBA in M7 and w...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2007-09-01 00:00:00
abstract::Present investigation was undertaken to evaluate antihyperglycemic, antihyperlipidemic and antioxidant activities of Dihar, a polyherbal formulation containing drugs from eight different herbs viz., Syzygium cumini, Momordica charantia, Emblica officinalis, Gymnema sylvestre, Enicostemma littorale, Azadirachta indica,...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Radiosensitizing effects of combination of a minor groove DNA ligand, Hoechst-33342, with the glucose analogue and inhibitor of glycolysis, 2-deoxy-D-glucose (2-DG) have been investigated in Ehrlich ascites tumour (EAT) bearing mice following focal irradiation of the tumour with 60Co gamma-rays. Treatment-induced tumo...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::We have found and synthesized a trapping ligand peptide H22-LP (the conservative sequence is NAHCALL) from a random phage library according to the broad-spectrum trapping receptor H22, which derived from the residue 14-35 near the N-terminal region of receptor US28 on HCMV. In this study, we will evaluate its potentia...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract::Protective effect of sex steroid hormones on aflatoxin-induced cytotoxicity on RBC (hemolysis) was examined in vitro. Addition of aflatoxin (2 micrograms/ml) in RBC suspension caused significant increase in hemolysis. Concurrent addition of aflatoxin (2 micrograms/ml) and estradiolbenzoate (100-1200 pg/ml) in RBC susp...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::The frequency and types of acrocentric chromosome association were quantitatively analysed in a Down syndrome child with unusual karyotype, 46, XX, -14, -22, t dic (14p;22p), +21, 21S+. Father and 4 sibs were heterozygous carriers for t dic (14p;22p). The variant 21S+ was inherited from the mother. The occurrence of t...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::The investigations on enzymes related to glutathione like glutathione-S-transferase (GST) and glutathione peroxidase (GSH-Px) have been carried out mostly in human and rat ovaries, however the studies on these enzymes in ruminants are relatively absent. In the present study the changes in the activity of these enzymes...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::Four plants having known medicinal properties were screened for inhibition of goatpox virus (GTPV) replication in vitro. Of the 4 plants, extract of Acacia arabica (Babul) and Eugenia jambolana (Jamun) leaves had inhibition (%) 99.70 and 99.92 at their maximum non toxic concentrations, 99.93 +/- 0.38 and 1999.73 +/- 0...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2008-02-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2007-09-01 00:00:00