Abstract:
:L-Tryptophan (Trp) is an essential amino acid and its deficiency is involved in various pathologies. In this present investigation an attempt was made to study the role of tryptophan and its metabolites in cataract formation in wistar rats. Rats were divided and maintained in 3 groups, Group A--control; Group B--marginal-tryptophan and Group C--Tryptophan-deficient diet for 3 months. Slit lamp microscope observations indicated lenticular opacities in Group-C (tryptophan-deficient) rats. In the rats that were maintained on tryptophan deficient diet, a decrease in protein content, kynurenines, reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione-s-tranferase (GSTs) and tryptophan-fluorescence intensities and an increase in lipid peroxidation indicative of oxidative stress have been observed. The above changes were normalized in the rats on supplementation of 0.05% tryptophan (Group-B) in their diets. These results suggest that tryptophan-deficiency in the diet leads to an overall significant decrease in kynurenines and levels of antioxidant enzymes (except SOD) in ocular tissue with a concomitant lenticular opacification. The results suggest that diet with adequate tryptophan has protective influence and is of immense benefit in mitigating the changes that may otherwise contribute to the lenticular opacities.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Raju TN,Kanth VR,Reddy PUsubject
Has Abstractpub_date
2007-06-01 00:00:00pages
543-8issue
6eissn
0019-5189issn
0975-1009journal_volume
45pub_type
杂志文章abstract::The sites of methionine uptake by 10 day lactating mouse mammary gland were determined in vitro. Four modes of methionine entry characterised were: (i) A sodium-dependent, N-(methylamino) isobutyric acid (MeAIB)--sensitive system with a Vmax of 18.8 nmol/g cells/min (this mode of entry was similar to the A site in oth...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::Tuberculin skin test (TST), an age old method is based on measuring delayed-type hypersensitivity (DTH) response to purified protein derivative (PPD). However, inspite of simplicity, ease and cost effectiveness, the usefulness of PPD test is limited due to its inability to distinguish among a protective immune respons...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Most phenolic substances of plant origin are toxic to microorganisms and they confer some degree of protection to plants against phytopathogens. Xanthomonas oryzae pv. oryzae, bacterial blight pathogen of rice (Oryza sativa) was treated with phenol (monohydroxy benzene) and its effects on the morphology and cytologica...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::A cobalt-resistant wall-less mutant of N. crassa (Cor-sl) characterized previously was also found to be 3-fold more resistant to nickel when compared to the parent wall-less mutant (W-sl). The Cor-sl strain accumulates relatively lower amounts of nickel when compared to W-sl. Sub-cellular fractionation showed signific...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract::The present study is planned to assess the diagnostic relevance of silver stained nucleolar organizer regions (NORs) in discriminating benign from malignant lesions of the breast and to ascertain the interobserver variability in the enumeration of AgNOR counts. Forty four specimens of benign and malignant breast tissu...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::The [125I]Insulin binding and receptor kinase activity were assessed in rat brain in the presence of 10 microM concentrations of the beta agonist isoproterenol. While insulin binding remained unaltered, beta agonist treatment enhanced significantly receptor kinase activity in control and hyperglycaemic conditions. Ant...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Limb remote ischemic postconditioning (LRIP) can reduce ischemia-reperfusion injury (IRI), but its mechanisms are still unclear. We hypothesize that LRIP reduces IRI by reversing eNOS uncoupling. Focal ischemia was induced in Sprague-Dawley rats by middle cerebral artery occlusion for 2 h followed by a 24 h reperfusio...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract::Stocks of three Indian Chandipura virus (CHPV) isolates; one isolate from an adult febrile case in 1965 from Chandipura town, Maharashtra, and two isolates from two pediatric encephalitis cases from Andhra Pradesh, 2003 were inoculated in 10-day-old chick embryos by allantoic route. All three virus isolates replicated...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract::Breast cancer susceptibility gene (BRCA1) is known to be responsible for hereditary breast and ovarian cancer. This gene is highly penetrant conferring a risk for 0.92 by the age of 70. Germline mutation in this gene leads to susceptibility to breast and ovarian cancer, with a genotype phenotype correlation. Frequency...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2001-05-01 00:00:00
abstract::Thirteen strains of M. bovoculi, 6 from frozen bull-semen (3.5% of 168), 3 from neat bull-semen (3.0% of 100), one each from heart blood and stomach contents of aborted foetus of 85 (1.18%) bovine-abortions, one each from stomach contents and pooled internal organs of 9 (11.1%) stillborn calves, were isolated. All the...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::Salmonella pathogenesis is a complex phenomenon and a Type III secretion system plays a central role in the development of Salmonella-induced enteritis. One such Type III secretion protein is Salmonella outer protein E (SopE). Prevalence of sopE gene and its phenotypic expression (SopE protein) among different serovar...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::A simple dot-immunobinding assay (Dot-Iba) in nitrocellulose paper was developed for the detection of specific IgG antibody to Mycobacterium tuberculosis antigen 5 and mycobacterial antigen in cerebrospinal fluid of patients with tuberculous meningitis (TBM). The assay gave 77.1% sensitivity for the detection of IgG a...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::The neutral detergent fiber (NDF) isolated from coconut kernel was digested with cellulase and hemicellulase and the residual fiber rich in hemicellulose (without cellulose) and cellulose (with out hemicellulose) were fed to rats and compared with a fiber free group. The results indicate that hemicellulose rich fiber ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Eclosion rhythm parameters of D. ananassae strains originating between 8 degrees-34 degrees N were highly variable and latitude dependent. In the field under naturally fluctuating light intensity, temperature and R.H., the amplitude of the rhythm was high and the eclosion gate was narrow; however, under the naturally ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::In the present investigation, growth of the organisms was reduced due to presence of arsenic (III) and (V) in the culture medium. In comparison to arsenic (V), arsenic (III) had more toxic effect on microalgae. Among the different algal strains, blue green algal species Oscillatoria-Lyngbya mixed culture showed maximu...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::Staphylococcus sp. strain BP/SU1, capable of degrading the biopolymer and utilize it as a source of carbon and energy, was isolated from activated sludge using METABOLIX (MBX D411G). It was found that this strain was capable of accumulating poly(3-hydroxybutyric acid) P(3-HB), as granule poly (3-hydroxybutyric acid), ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2009-04-01 00:00:00
abstract::Getting higher yields of monoclonal antibody (MAb) is a problem in Hybridoma Technology which has two major bottlenecks--(a) poor yield of hybridized cells; and (b) low cellular productivity of MAb in culture. There are three ways of obtaining high MAb yield in vitro--(a) large scale culture of hybrid cells; (b) high ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:1998-02-01 00:00:00
abstract::In this study, we explored the effects of consumption of banana in thirty hypercholesterolemic and fifteen type 2 diabetic subjects. They were given a daily dose of 250 or 500 grams of banana for breakfast for 12 weeks. Fasting serum lipid, glucose and insulin levels were measured initially as well as every 4 weeks. D...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2014-12-01 00:00:00
abstract::Effects of exogenous androgens (testosterone, testosterone propionate and dihydrotestosterone) and estradiol-17beta on the oviductal growth/hypertrophy were studied in young and bilaterally ovariectomized (BLO) adult frogs (Rana cyanophlyctis) during postbreeding phase of the reproductive cycle. Estradiol-17beta injec...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Indian toad (Bufo melanostictus, Schneider) skin extract (TSE) is pharmacologically potent and probably contains several bioactive compounds [Das et. al., Indian J Pharmacol, 28 (1996) 72]. A lethal factor was isolated and purified by neutral alumina column chromatography followed by HPLC. Spectroscopic (UV, IR, FAB-M...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::The high intake of refined sugars, mainly fructose has been implicated in the epidemiology of metabolic diseases in adults and children. With an aim to determine whether honey can substitute refined sugars without adverse effect, the long-term effects of natural honey and cane syrup have been compared on visceral morp...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2013-04-01 00:00:00
abstract::Extract of the flower Malva viscus conzattii (M. conzattii) was administered at a dose of 25/50 mg/day/animal to 30 healthy adult male gerbils and 30 adult male house rats to determine its effect on fertility. After 25 days' treatment fin l body weight, and the weights of testes, epididymides, seminal vesicles, and a...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1977-07-01 00:00:00
abstract::The antifungal activity of saponins isolated as a byproduct from the defatted cake of M. butyracea oil seed is reported. The inhibitory concentrations against plant pathogenic fungi ranged from 500 to 2000 ppm. Maximum sensitivity to saponins was shown by Penicillium expansum. Cephalosporium acrimonium, Helminthospori...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Efforts have been made to minimize the toxic effect caused by beryllium. Adult cyclic rats of Sprague Dawley strain were administered a bolus dose of 50mg/kg beryllium nitrate intramuscularly. The chelation therapy with glutathione (GSH), dimercapto propane sulfonic acid (DMPS)+ selenium (Se) and D-Penicillamine (DPA)...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::Reperfusion injury is remarkable clinical issue that needs to be resolved as ischemia-reperfusion is a common phenomenon encountered in numerous clinical situations. The present communication report the involvement of nitric oxide (NO) in cardioprotection offered by flavonoids (rutin and quercetin) against myocardial ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2011-03-01 00:00:00
abstract::Thermoactinomycetes vulgaris is a thermophilic actinomycetes growing optimally at 50 degrees C and Streptomyces albus, S. coelicolor, S. fasciculus and S. olivochromogenes are thermophilically disposed transition species of actinomycetes, which have optimum biomass at 40 degrees C. The acid/alkaline phosphatase and ac...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2011-12-01 00:00:00
abstract::Dicarboxylic acids and a few amino acids were found to support mitochondrial phosphorylation in A. ceylanicum. Anaerobiasis markedly reduced this activity. Maximum effect was observed on succinate supported phosphorylation which in anaerobic atmosphere yielded only 2% ATP compared to that in the presence of air. Known...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract::Administration of ethanol extract of stem bark from Z. rhoifolium (EEtOH-ZR) induced hypotension associated with a dual effect in heart rate in normotensive rats. This response was highlighted in spontaneously hypertensive rats (SHR). In rat superior mesenteric artery rings, the cumulative addition of EEtOH-ZR (0.1-75...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2013-08-01 00:00:00
abstract::Amines such as dopamine, norepinephrine and epinephrine were analysed in the brain regions of O.mossambicus exposed to quinalphos, phenthoate and their combination for 96 hr. The three types of treatments significantly (P < 0.05) altered the amines level at various intervals in the brain regions. ...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00