In vitro capacitation of goat spermatozoa.

Abstract:

:Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.

journal_name

Indian J Exp Biol

authors

Anand SR,Atreja SK,Chauhan MS,Behl R

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

921-4

issue

11

eissn

0019-5189

issn

0975-1009

journal_volume

27

pub_type

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