Abstract:
:Goat epididymal and ejaculated spermatozoa were incubated in Krebs-Ringer bicarbonate buffer containing pyruvate and lactate as energy source. A 3 hr incubation for epididymal and 4 hr for ejaculated spermatozoa was required for the capacitation and acrosome reaction to take place. Calcium is an essential requirement which was needed for motility maintenance/activation and for the initiation of acrosome reaction. A 2-fold increase in cAMP content was measured over 3 hr period of incubation of goat epididymal spermatozoa which was not seen when calcium ions were either omitted or chelated with EGTA. There is thus a definite involvement of Ca2+ ions and cAMP in capacitation and acrosome reaction of goat spermatozoa.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Anand SR,Atreja SK,Chauhan MS,Behl Rsubject
Has Abstractpub_date
1989-11-01 00:00:00pages
921-4issue
11eissn
0019-5189issn
0975-1009journal_volume
27pub_type
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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doi:
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pub_type: 杂志文章,评审
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