Abstract：
:Aeromonas sp. from Lamellidens marginalis produced L-asparaginase when grown at 37 degrees C. The optimum enzyme activity was at pH 9 when temperature was 45 degrees C. Half-life of partially purified enzyme at 50 degrees C and 55 degrees C was 35 and 20 min, respectively. Activation and deactivation energies of partially purified enzyme were 17.48 and 24.86 kcal mol-1 respectively. The enzyme exhibited a Km (L-asparagine) value of 4.9 x 10(-6) mol l-1 and a Vmax of 9.803 IU ml-1. Three metal ions inhibited the enzyme activity at 10-20 mumol l-1 concentrations. Catalytic activity was also inhibited by EDTA, iodoacetic acid, parachloromercuribenzoic acid and phenylmethylsulphonyl fluoride at 0.1 mumol l-1.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Pattnaik S,Kabi R,Janaki Ram K,Bhanot KKkeywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
1143-6issue
11eissn
0019-5189issn
0975-1009journal_volume
38pub_type
杂志文章abstract：:Effects of sublethal doses of metal (Cu, Cd, Zn) mixtures on the activities of key respiratory enzymes (succinate dehydrogenase, SDH and glyceraldehyde dehydrogenase, GDH) and their recovery following withdrawal of treatments were studied in the freshwater fish O. mossambicus. On the basis of 96 hr LC50 Cu was highly ...
journal_title：Indian journal of experimental biology
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更新日期：2007-12-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：1992-07-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：1999-04-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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doi：
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更新日期：2003-07-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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更新日期：1994-07-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
