Abstract：
:Anticancer potential of Moringa oleifera L. extracts have been well established. However, there are no reports on the isolated molecules/fractions from these extracts which are responsible for the anticancer/cytotoxic activity. Thus, in the present study, we explored the same. The n-hexane, chloroform, ethyl acetate, methanol extracts of the M. oleifera leaves and 15 fractions (F1 to F15) of ethyl acetate extract were evaluated for their in vitro and in vivo anticancer activity using Hep-2 cell lines and Dalton's lymphoma ascites model in mice, respectively. Among the tested samples, the F1 fraction showed potential cytotoxic effect in Hep-2 cell lines with a CTC50 value of 12.5 ± 0.5 μg/ml. In vivo studies with the doses 5 and 10 mg/kg, p.o. demonstrated significant reduction in body weight and increased the mean survival time compared to the control group. These results were also comparable to the standard, 5-Fluorouracil, treated animals. We have also successfully isolated and characterized the anticancer fraction, F1 from the leaves of M. oleifera L.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Krishnamurthy PT,Vardarajalu A,Wadhwani A,Patel Vsubject
Has Abstractpub_date
2015-02-01 00:00:00pages
98-103issue
2eissn
0019-5189issn
0975-1009journal_volume
53pub_type
杂志文章abstract：:Eclosion rhythm parameters of D. ananassae strains originating between 8 degrees-34 degrees N were highly variable and latitude dependent. In the field under naturally fluctuating light intensity, temperature and R.H., the amplitude of the rhythm was high and the eclosion gate was narrow; however, under the naturally ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Maize (Zea mays L. cv kanaujia) plants grown with Zn [10 (control), 0.1 (low) and 20 microM (high)], were investigated for concentration of antioxidants and activities of antioxidative enzymes in leaves. Young leaves of low Zn plants developed whitish-necrotic spots. Leaves of both low and high Zn plants showed decrea...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-08-01 00:00:00
abstract：:Aqueous extract of Andrographis paniculata was examined for antioxidant activity using rat liver subcellular organelles as model systems. The study deals with two important biological oxidative agents, ascorbate-Fe(+2) and AAPH generating hydroxyl and peroxyl radical, respectively. Oxidative damage was examined agains...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-11-01 00:00:00
abstract：:This study was designed to check whether insulin supplementation is crucial for inducing diabetic nephropathy (DNP) in Wistar rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin. The urinary biochemical parameters such as albumin, creatinine and urea nitrogen were monitored every two wee...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：:The present study is planned to assess the diagnostic relevance of silver stained nucleolar organizer regions (NORs) in discriminating benign from malignant lesions of the breast and to ascertain the interobserver variability in the enumeration of AgNOR counts. Forty four specimens of benign and malignant breast tissu...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-10-01 00:00:00
abstract：:Fluorescent pseudomonads were isolated from the rhizosphere of rice and sugarcane and examined for their biodiversity. All fifty strains of the fluorescent pseudomonads produced indole acetic acid. Among these pseudomonads, halves of sugarcane rhizosphere isolates and one isolate from the rice rhizosphere exhibited ph...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Forty days of orally feeding the aqueous E. ribes extract (100 and 200 mg/kg) to streptozotocin (40 mg/kg, iv, single dose) induced diabetic rats produced significant decrease in heart rate, systolic blood pressure, blood glucose, blood glycosylated hemoglobin, serum lactate dehydrogenase, creatine kinase and increase...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:Lipopolysaccharide (LPS) and ratio-detoxified LPS (Rd-LPS) from Salmonella typhimurium were analysed for their ability to stimulate murine peritoneal exudate cells (PEC) and macrophages. Rd-LPS induced much more inflammatory response as compared to LPS. PEC numbers/mouse obtained were significantly higher (3-fold) in ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:A protocol has been devised for enhanced in vitro regeneration of critically endangered Mantisia spathulata Schult. and Mantisia wengeri Fischer. Highest Bud Forming Capacity (BFC) of 6.10 +/- 0.55 with an average of 19.93 +/- 3.19 roots was obtained for M. spathulata within 5-6 weeks in Murashige and Skoogs (MS) medi...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
abstract：:Urokinase-type plasminogen activator(uPA) assayed in uteri of cycling and deciduoma bearing rats shows detectable levels of this enzyme in endometrium. Zymographic analysis confirms uPA of decidual tissue and, following artificial induction of decidualisation, uPA activity constantly increases in the decidualising end...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
abstract：:Seed characteristics and in vitro culture of C. tamala embryos were studied. Embryos desiccated below 50% (fresh weight) exhibited poor morphogenetic response in vitro and confirmed the recalcitrant nature of seeds. The immature embryos of various developmental ages (4-16 week after flowering, WAF) were cultured on di...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2014-10-01 00:00:00
abstract：:Estrogenic and antiestrogenic properties of E-492, a nonsteroidal compound (3 methyl-4'-(beta-pyrrolidinoethoxy)-2,3-diphenyl propiophenone), were assessed on the basis of ponderal, histologic, and biochemical changes in the uterus, cervix, and vagina of ovariectomized adult rats. This compound was studied at its max...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Isoproturon, a nonhalogenated substituted phenylurea herbicide, was evaluated for its cumulative toxic effects on testicular histomorphology., steroid hormone biosynthesis-related enzymes, spermatogenesis and sperm cells in adult albino rats. The compound, suspended in refined groundnut oil, was administered (po) to r...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:The 50% ethanolic extract of the root bark of C. odorata administered orally at the dose of 1g/kg body weight/day for 60 days resulted in decreased epididymal sperm motility and sperm count in male albino rats. Morphological abnormalities were also observed in the sperms. The testicular glycogen, the activities of 3be...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2006-12-01 00:00:00
abstract：:Leydig cells are the primary source of androgens in the mammalian testis. It is established that the luteinizing hormone (LH) produced by the anterior pituitary is required to maintain the structure and function of the Leydig cells in the postnatal testis. Until recent years, a role by the thyroid hormones on Leydig c...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2005-11-01 00:00:00
abstract：:Although peripheral axotomy of dorsal root ganglion cells in mice, rats and cats has been reported to generate spontaneous activity in sensory nerves, we did not find evidence for such activity in the hamster. In vitro, intracellular recording from L6-S1 dorsal root ganglion cells up to 6 weeks after axotomy did not r...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Xenobiotics have played a role in elucidating the regulation of gene expression of hepatic cytochrome P450 in the eukaryotes. The major regulation of P450 genes in the eukaryotes is at the transcriptional and post transcriptional level. Polycyclic aromatic hydrocarbons regulate the gene expression by binding the cytos...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1998-05-01 00:00:00
abstract：:In the present study, antibacterial activity of aqueous and organic extracts of Psidium guajava leaves was evaluated against multidrug resistant (MDR) clinical isolates of Staphylococcus aureus strains collected from hospitals in northern (Malabar region) Kerala. The strains which exhibited resistance against all the ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Progesterone, primarily recognized as a female steroid hormone, is reported to affect several sperm functions especially capacitation, motility and acrosome reaction. These effects of progesterone on the spermatozoa are mediated via the progesterone binding sites/progesterone receptor (PR) on the acrosomal membrane. T...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：2003-07-01 00:00:00
abstract：:N-formyl-methionyl-leucyl-phenylalanine (fMLP) a potent chemotactic peptide stimulates immune responses by activating macrophages and other cells of the immune system. The present study reports inhibition of fMLP-induced activation of murine peritoneal and P388D-1 macrophage cell line by protein kinase C (PKC) inhibit...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:Cognitive disorders in mankind are not uncommon. Apart from neurodegenerative diseases such as Alzheimer's (AD), various stresses also affect cognitive functions. Plants are known to be potential source of compounds that ameliorate several diseases including cognitive impairment. Here, we evaluated effect of aqueous e...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2016-02-01 00:00:00
abstract：:One day and 7 days old male white leghorn chicks were administered vitamin E prior to their exposure to sublethal dose (2.25 Gy) of gamma radiation from 60C source. The results show that vitamin E helps in faster recovery of damage in chick thymus caused by irradiation. ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:The sites of methionine uptake by 10 day lactating mouse mammary gland were determined in vitro. Four modes of methionine entry characterised were: (i) A sodium-dependent, N-(methylamino) isobutyric acid (MeAIB)--sensitive system with a Vmax of 18.8 nmol/g cells/min (this mode of entry was similar to the A site in oth...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:Mechanism of microtuberization in three elite cultivars kufri badhsha (KB), kufri chandramukhi (KCM) and kufri jawahar (KJ) of potato was studied. Sprouts of all the three cultivars were used to obtain in vitro shoot cultures. MS medium supplemented with chlorocholine chloride was found to be most suitable for all the...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2004-12-01 00:00:00
abstract：:O. mossambicus was exposed to sublethal concentrations of a textile mill effluent to study the hematological changes as a function of feeding/starvation, aeration/non-aeration and concentration. Increase in concentration produced a dose dependent increase in RBCs (0.87 x 10(6) to 1.63 x 10(6)/mm3), WBCs (0.070 x 10(6)...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:Sodium valproate (VPA) and flunarizine (FLU) administered individually and together were examined for their effects on behavioural, and EEG changes in the pentylenetetrazole (PTZ) induced rat model of absence seizures. PTZ, 20 mg/kg, i.p., produced behavioural staring and immobility with concomitant, repetitive 7 to 9...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Chinese hamster V79 cells were repeatedly exposed to a low dose of hydrogen peroxide (H2O2) over several weeks and then exposed to H2O2, cisplatin or ultraviolet (UV) light. Cell killing was examined by colony formation, following these treatments. It was seen that cells conditioned by multiple low doses of H2O2 showe...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：:Administration of single dose (175 mg/kg body wt) of amiodarone dissolved in water through gavage for 3 weeks damaged the lung and changed the content of lung lavage. Activities of bronchoalveolar lavage (BAL) angiotensin converting enzyme (ACE) and lactate dehydrogenase (LDH) increased significantly. Also, the protei...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:The p38 mitogen activated protein kinase (p38MAPK) pathway is an important signaling cascade involved in cell growth, differentiation and apoptosis. High glucose activates p38MAPK pathway in different cells, including osteoblasts. In the present study, role of p38MAPK in high glucose induced osteoblast apoptosis and p...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2011-02-01 00:00:00
abstract：:Bovine leukemia virus (BLV), like its closest relatives human T-cell leukemia virus-I and II, contain a 'px' gene, between the 'env' gene and the 3' long terminal repeat in its genome. A monoclonal antibody prepared against a synthetic oligopeptide whose sequence was deduced from highly conserved region of 'px' gene o...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
