Abstract:
:Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Li Y,Wang W,Liu X,Luo W,Zhang J,Gul Ysubject
Has Abstractpub_date
2011-12-01 00:00:00pages
953-7issue
12eissn
0019-5189issn
0975-1009journal_volume
49pub_type
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