Abstract：
:Valeriana officinalis is an important medicinal herb commonly found in Kashmir valley. This study forms an important preliminary step for in-vitro micro propagation of V. officinalis from breaking the seed dormancy, inducing rapid seed germination and its subsequent micro propagation. We investigated the influence of pretreatment of V. officinalis seeds with reduced temperature and light on seed germination and in-vitro propagation. Culture of explants from cultivated seeds have demonstrated its potential for in vitro propagation and plantlet regeneration. Individual as well as combinations of treatments such as temperature and light availability influenced the germination of seeds variedly. Unchilled seeds of V. officinalis were given dip in GA3 (200 ppm) for 24, 48 and 120 h. Seeds treated with GA3 for 24 h and kept in darkness showed the best results, i.e. 48%. Seeds pretreated with GA3 for 120 h and incubated in dark showed 40% germination. Pre-chilling up to 72 h and kept in light showed maximum germination of 60% followed by 40% kept in darkness. Pre-chilling for 48 h resulted in 40 and 25% seed germination in light and darkness, respectively. GA3 pre-treatment for 72 h and 24 h pre chilling were most effective in inducing seed germination. Maximum shoot response was obtained on MS enriched with BAP (1 mg/L) + IAA (0.1 mg/L) combinations using shoot tips as explants. Multiple shoot regeneration from shoot apices was recorded on BAP (1 mg/L) and BAP (1 mg/L) + IAA (0.1 mg/L).
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Bhat B,Sharma VDsubject
Has Abstractpub_date
2015-03-01 00:00:00pages
184-8issue
3eissn
0019-5189issn
0975-1009journal_volume
53pub_type
杂志文章abstract：:Toxic bait discs were prepared by incorporating the larvicide Abate (Temephos) technical, with yeast and dog biscuit (1:1), the latter forming the attractant source. These were assessed in 1, 10 and 25 litre aquaria and 100 litre drums against Aedes aegypti larvae. Floating as well as sinking bait discs were found equ...
journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：1998-12-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
更新日期：2000-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
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journal_title：Indian journal of experimental biology
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更新日期：1994-05-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：2002-07-01 00:00:00
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更新日期：2000-03-01 00:00:00
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更新日期：2013-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章,评审
doi：
更新日期：1998-02-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：2003-04-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：1990-06-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
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journal_title：Indian journal of experimental biology
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doi：
更新日期：1995-12-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：2001-03-01 00:00:00
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journal_title：Indian journal of experimental biology
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更新日期：2000-12-01 00:00:00
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journal_title：Indian journal of experimental biology
pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
