Abstract：
:A genetic locus D1S80 containing a variable number of tandem repeats (VNTR) has been used extensively in forensic analysis and paternity testing. In the current research, the D1S80 locus was amplified using polymerase chain reaction (PCR) technology and the alleles detected using a high sensitivity infrared (IR) fluorescence automated DNA sequencer. IR-labeled amplification products were generated using oligonucleotide primers which were covalently linked to an infrared fluorescent dye (IRD41) at the 5'-end. Human genomic DNA (1.0 ng or less) isolated from blood and various simulated forensic samples was successfully amplified using this technology. Allelic bands were detected by incorporation of the IR fluorescent dye into PCR products. Both Long Ranger and polyacrylamide denaturing gels permitted clear resolution of individual alleles that differ by only one repeat unit. In the smaller gels a separation distance of only 15 cm allowed separation of the alleles in less than 2 h from sample loading to visualization. This system combines IR fluorescence chemistry and laser technology thus eliminating the need for post-electrophoretic gel handling for the detection of D1S80 alleles. Real-time detection is valuable for immediate visualization of the data and the alleles are displayed as familiar autoradiogram-like images which can also be analyzed by computer. By loading a 64-lane gel twice it is possible to type at least 120 samples in 1 day using a single gel.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Roy Rdoi
10.1016/s0379-0738(97)00043-1subject
Has Abstractpub_date
1997-05-23 00:00:00pages
63-71issue
1eissn
0379-0738issn
1872-6283pii
S0379-0738(97)00043-1journal_volume
87pub_type
杂志文章abstract：:Gladiator remains from a recently unearthed cemetery in ancient Ephesus (Turkey) offer a unique opportunity for proving common theories involving the weaponry and techniques of gladiator fighting based on the evidence supplied by cranial bones. This mass grave is the first of its kind to undergo a thorough osteologica...
journal_title：Forensic science international
pub_type： 历史文章,杂志文章
doi：10.1016/j.forsciint.2005.10.010
更新日期：2006-07-13 00:00:00
abstract：:The 4977 bp deletion of mitochondrial DNA (mtDNA) accumulates in postmitotic tissues with advancing age. The purpose of our study was to detect and quantify these deletion even in blood cells with a high turnover activity. Whole venous blood, isolated human platelets and peripheral blood mononuclear cells (PBMCs) were...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00249-8
更新日期：2000-09-11 00:00:00
abstract：:Our aim was to investigate the reason for relatively low detection rates for opioids and fentanyl in particular in post-mortem cases in the State of Hamburg. We re-analysed 822 blood samples from two different time periods, 2011/12 and 2016. These samples had been previously analysed in accordance with post-mortem rou...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2019.109970
更新日期：2019-12-01 00:00:00
abstract：:Since gamma-hydroxybutyrate (GHB) is present in both blood and urine of the general population as an endogenous compound, toxicologists must be able to discriminate between these endogenous levels and a concentration resulting from exogenous exposure. The implementation of a cut-off concentration must be done cautious...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.02.036
更新日期：2004-07-16 00:00:00
abstract：:Extrahepatic biliary atresia (EHBA) is a rare disease characterized by progressive and obliterative cholangiopathy in infants and is one of the major causes of secondary vitamin K deficiency bleeding (VKDB) due to cholestasis-induced fat malabsorption. Breast feeding increases the tendency of bleeding in EHBA patients...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.04.018
更新日期：2012-09-10 00:00:00
abstract：:Allele frequencies for the 15 STRs included in the AmpFlSTR SGM Plus and Profiler kit (D3S1358, VWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, THO1, FGA, TPOX, CSF1PO, D5S818, D13S317 and D7S820) were obtained from a sample of 597 unrelated individuals living in Taiwan. All loci, except for D8S1179 and D5S81...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2003.09.006
更新日期：2003-12-17 00:00:00
abstract：:The Purkinje-cells (PCs) of the cerebellum are highly vulnerable to hypoxic/ischemic insult. Calbindin-D28k is a calcium-binding protein that is strongly expressed in PCs. Following hypoxia, a decrease in its concentration has been found in animal models before any morphological change of the PCs took place. Hypoxia-i...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.08.023
更新日期：2012-11-30 00:00:00
abstract：:This paper reports results from a field trial with a breath-alcohol screening device--Alcolmeter pocket model. Breath tests were made with drivers apprehended during routine controls (road-blocks), for traffic violations and those involved in traffic accidents. Of 908 roadside breath tests made with chemical reagent t...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(85)90126-4
更新日期：1985-08-01 00:00:00
abstract：:In the Middle European medico-legal climate, the moral rule 'salus aegroti suprema lex' has been accepted for a long time. In the last few years, under the pressure of fear of accusation of a paternalistic attitude, this postulate has been changed to 'voluntas aegroti suprema lex'. The question stands: Is this valid i...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(94)90399-9
更新日期：1994-12-16 00:00:00
abstract：:The objective of this study was to evaluate the applicability of human identification parameters, established by Tatlisumak et al. (2007), by means of cone-beam computed tomography and extraoral radiographs of the frontal sinus region. From a total of 58 dry skulls, 26 were selected. Posteroanterior, profile cephalome...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.03.030
更新日期：2016-05-01 00:00:00
abstract：:A simple and rapid method for analysis of free and conjugated cresols in biological fluids was developed. Prior to and following freeing of the conjugated cresols by acid hydrolysis in a sealed ampoule, free cresols were extracted by Extrelut column extraction, determined by gas chromatography, and confirmed by gas ch...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(90)90282-4
更新日期：1990-08-01 00:00:00
abstract：:In cases of criminal thallium poisoning, forensic investigation is required to identify the amount and time of thallium exposure. Usually, blood and urine thallium levels are respectively used as biomarkers. Additionally, hair has the unique potential to reveal retrospective information. Although several studies have ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2018.08.019
更新日期：2018-10-01 00:00:00
abstract：:This paper introduces a set of methods for image and video forensic analysis. They were designed to help to assess image and video credibility and origin and to restore and increase image quality by diminishing unwanted blur, noise, and other possible artifacts. The motivation came from the best practices used in the ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2016.04.027
更新日期：2016-07-01 00:00:00
abstract：:Numerous studies have mentioned to the high percentage of violent deaths in prison psychiatric hospitals, with suicide being the principal cause. The aim of this study was to analyze the circumstances related with the deaths recorded in a high security institution. Postmortem reports on all the deaths at the Alicante ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/s0379-0738(00)00441-2
更新日期：2001-07-15 00:00:00
abstract：:This work examines the synthesis of 3,4-methylenedioxy-N-methylamphetamine (MDMA) from common starting materials that may be utilised by clandestine laboratory operators. Piperonal was prepared from two common starting materials, piperine (from pepper) and vanillin (a common flavouring). Piperine was converted to pipe...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.10.006
更新日期：2012-11-30 00:00:00
abstract：:This is the first controlled study of torture victims published. Ten persons who alleged subjection to torture were examined by two different medical teams during the first months after torture. The testimonies of the probands were found to be credible and the method of examination was found to be reliable. The state ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(85)90111-2
更新日期：1985-11-01 00:00:00
abstract：:Positive (certain, absolute) identification of human remains needs a scientific comparison between ante mortem and post-mortem biologic features, as fingerprint, odontological, radiological or DNA comparisons. X-rays comparison has been extensively used, usually comparing some peculiarities such as outlines of the bon...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2014.09.019
更新日期：2014-12-01 00:00:00
abstract：:This report highlights the importance of an individual chemical's odor impact in the olfactory identification of marijuana, cocaine, and heroin. There are small amounts of highly odorous compounds present in headspace of these drugs, with very low odor detection thresholds, that are more likely responsible for contrib...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2015.08.027
更新日期：2015-12-01 00:00:00
abstract：:Carbofuran is a broad spectrum carbamate insecticide which inhibits cholinesterase. In the recent past several carbofuran related homicide and suicide cases were reported in Sri Lanka. The concentration of carbofuran found in blood, bile and/or tissues in eight fatal cases were presented in this paper. Liquid-liquid e...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.10.039
更新日期：2013-04-10 00:00:00
abstract：:Obtaining a genetic profile from pieces of evidence collected at a crime scene is the primary objective of forensic laboratories. New procedures, methods, kits, software or equipment must be carefully evaluated and validated before its implementation. The constant development of new methodologies for DNA testing leads...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2017.11.037
更新日期：2018-02-01 00:00:00
abstract：:Many studies in the forensic field have reported that analysis of DNA methylation is the most reliable method of predicting age. In a previous study, 5 CpG sites located in ELOVL2, FHL2, KLF14, C1orf132 and TRIM59 genes were tested for age prediction purposes in blood, saliva and buccal swab samples from Korean indivi...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2020.110267
更新日期：2020-06-01 00:00:00
abstract：:During a 3-year period (August 1st, 1985 to July 31st, 1988) a systematic investigation of medico-legal autopsy cases with regard to the presence of antibodies for HIV-virus was carried out at the Department of Forensic Medicine in Stockholm, Sweden. Prior to autopsy, blood samples were taken from femoral or subclavia...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(89)90220-x
更新日期：1989-06-01 00:00:00
abstract：:A high-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) technique was developed for the simultaneous determination of five non-steroidal anti-inflammatory oxicam drugs (ampiroxicam, tenoxicam, piroxicam, meloxicam and lornoxicam) in human plasma. These five oxicam drugs and isoxicam (internal stan...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2012.11.016
更新日期：2013-04-10 00:00:00
abstract：:Fibronectin has been proposed as the reliable marker of wound vitality and potent tool for age estimation in cases where survival time is at least few minutes. Cases of suicidal gunshot wounds were divided into two groups according to projectile energy and damage of the vital structures. Immunohistochemical reaction o...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2004.09.081
更新日期：2005-01-17 00:00:00
abstract：:The objective of this study was to supply information about: (1) normal sex-related dimensions of mouth and lips (linear distances, ratios, angles, area, volume); and (2) growth changes between childhood and old age. The three-dimensional coordinates of several soft-tissue landmarks on the lips and face were obtained ...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.04.050
更新日期：2010-07-15 00:00:00
abstract：:The Forensic Anthropology Service of the Medical Examiner's Office of Porto Alegre was created in September 1997 to examine human skeletons, establish identification and identify lesions that may explain death. From September 1997 to December 2006, 344 skeletons were examined (mean: 37 examinations/year), and 322 were...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2008.05.004
更新日期：2008-08-06 00:00:00
abstract：:A urine screening in fatalities with an unnatural or unknown cause of death (13-40 years of age) was carried out to evaluate the proportion of undetected drug-related cases. Ten out of 160 fatalities without a hint of drug consumption were classified as drug-related according to the definition of the Bundeskriminalamt...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/0379-0738(93)90062-f
更新日期：1993-11-01 00:00:00
abstract：:A recent publication in this journal [1] presented the results of a field study that revealed the data provided by the fingermarks not processed in a forensic science laboratory. In their study, the authors were interested in the usefulness of this additional data in order to determine whether such fingermarks would h...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2013.01.019
更新日期：2013-03-10 00:00:00
abstract：:Suicide attempts are one of the risk factors of suicide. Possible mechanisms by which Toxoplasma gondii may affect human behavior and it may also cause humans to attempt suicide. The aim of this study is to find out whether or not T. gondii is one of the reasons in suicide attempts. We investigated the sero-positivity...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2010.02.020
更新日期：2010-06-15 00:00:00
abstract：:Hypertension is a growing medical concern in the United States. With the number of Americans suffering from hypertension increasing, the use of antihypertensives such as beta-blockers is increasing as well. In fact, three beta-blockers - atenolol, metoprolol and propranolol - were among the 200 most prescribed medicat...
journal_title：Forensic science international
pub_type： 杂志文章
doi：10.1016/j.forsciint.2005.01.001
更新日期：2006-01-27 00:00:00
