Abstract:
:We have previously used antisense oligodeoxynucleotides (ODN) to the cloned delta opioid receptor (DOR) to inhibit the antinociceptive response to spinally administered delta opioid receptor selective agonists in mice. Here we have examined the effect of DOR antisense ODN treatment on the level of DOR expressed in NG 108-15 cells and the spinal cord, through immuno-fluorescence microscopy, to determine the efficiency and selectivity of the antisense ODN-mediated "knock-down' of the DOR in these tissues. Antisense ODN, but not mismatch control, treatment resulted in a significant reduction in DOR immunoreactivity (-ir) in NG 108-15 cells and spinal cord. Thus, the inhibition of antinociceptive response to intrathecal delta selective agonists by DOR antisense ODN correlates with the loss of DOR-ir in the superficial layers of the dorsal horn of the spinal cord.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Lai J,Riedl M,Stone LS,Arvidsson U,Bilsky EJ,Wilcox GL,Elde R,Porreca Fdoi
10.1016/0304-3940(96)12883-4subject
Has Abstractpub_date
1996-08-09 00:00:00pages
205-8issue
3eissn
0304-3940issn
1872-7972pii
S0304394096128834journal_volume
213pub_type
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