Detection and typing of human papillomavirus in biopsy and cytological specimens by polymerase chain reaction and restriction enzyme analysis: a method suitable for semiautomation.

abstract：:Previously, it was reported that productive viral infection, viral protein synthesis, and viral RNA replication of respiratory syncytial virus (RSV) operated efficiently in two human epithelial cell lines (HEp-2 and A549), but not in a human mast-cell line, HMC-1. Based on these observations, it was hypothesized that ...

journal_title：Journal of medical virology

authors： Shirato K,Ujike M,Kawase M,Matsuyama S

更新日期：2012-02-01 00:00:00

abstract：:Hepatitis C virus (HCV) exposure in blood donors is determined serologically by the detection of anti-HCV antibodies in serum or plasma. However, a "window" period of 30-70 days after exposure exists where specific antibodies to HCV antigens are not detected. The use of nucleic acid testing for the detection of HCV RN...

journal_title：Journal of medical virology

authors： Leary TP,Gutierrez RA,Muerhoff AS,Birkenmeyer LG,Desai SM,Dawson GJ

更新日期：2006-11-01 00:00:00

abstract：:Human herpesvirus 6 (HHV6) is a beta-herpesvirus capable of infecting several cell types from different origins. HHV6 is the etiological agent of exantem subitum and has been associated with several diseases, all characterized by an inflammatory response triggered by chemokines. We show that strain U1102 of HHV6 is ab...

journal_title：Journal of medical virology

authors： Caruso A,Favilli F,Rotola A,Comar M,Horejsh D,Alessandri G,Grassi M,Di Luca D,Fiorentini S

更新日期：2003-07-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) prototype isolate GS is a newly identified lymphotropic herpesvirus and several subsequent herpes isolates were recognized as HHV-6 by their hybridization to a HHV-6(GS) DNA probe pZVH14. DNA restriction analysis and in vitro tropism studies show that HHV-6 isolates can be divided into two ...

journal_title：Journal of medical virology

authors： Chandran B,Tirawatnapong S,Pfeiffer B,Ablashi DV

更新日期：1992-08-01 00:00:00

abstract：BACKGROUND:Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children in low and middle-income countries. Human metapneumovirus (hMPV) is one of the most common viral etiological agents for ARIs in children. OBJECTIVES:In this study, we explored the genotypic diversity and the epidemio...

journal_title：Journal of medical virology

authors： Rahman MZ,Sumiya M,Sahabuddin M,Pell LG,Gubbay JB,Rahman R,Momtaz F,Azmuda N,Shanta SS,Jahan I,Rahman M,Mahmud AA,Roth DE,Morris SK

更新日期：2019-04-01 00:00:00

abstract：:This study aimed at developing strategies for screening, predicting, and diagnosing intrauterine HBV infection in infants born to HBsAg positive mothers. A total of 1,360 infants born to 1,355 HBsAg positive mothers were followed for 1 year. All newborn infants received active and passive immunization within 24 hr aft...

journal_title：Journal of medical virology

authors： Yin YZ,Zhang J,Wu LL,Zhou J,Zhang PZ,Zhou SS

更新日期：2013-10-01 00:00:00

abstract：:The oncoprotein Tax was characterized genetically from a large cohort of human T-cell lymphotropic virus type 1 (HTLV-1) seropositive individuals from the most endemic region of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and HTLV-1 infection in Argentina, the province of San Salvador de Jujuy....

journal_title：Journal of medical virology

authors： Iñiguez AM,Gastaldello R,Otsuki K,Balangero M,Carvalho Costa F,Remondegui C,Paula Vicente AC,Gallego S

更新日期：2010-08-01 00:00:00

abstract：:To elucidate the mechanism of heterologous antibody responses to herpes simplex virus (HSV) and varicella-zoster virus (VZV) which occur in some patients with HSV or VZV infections, stronger evidence was sought for the existence of cross-reacting antibodies to these viruses, using antibody absorption procedures. Absor...

journal_title：Journal of medical virology

authors： Schmidt NJ

更新日期：1982-01-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) is classified as two distinct species: HHV-6A and B. HHV-6B infection can cause several clinical manifestations in transplant recipients including encephalitis, bone marrow suppression, and pneumonitis. In contrast to HHV-6B, the clinical features of HHV-6A infection remain largely undefine...

journal_title：Journal of medical virology

authors： Ihira M,Yamaki A,Kato Y,Higashimoto Y,Kawamura Y,Yoshikawa T

更新日期：2016-09-01 00:00:00

abstract：:Hepatitis E virus sequences were detected by RT-PCR in 14/15 (93%) of untreated sewage samples from Edinburgh, Scotland, UK. Phylogenetic analysis of amplicons at limiting dilution revealed the co-circulation of multiple variants of HEV-3, with a pattern of diversity matching that observed in a local cohort of HEV-inf...

journal_title：Journal of medical virology

authors： Smith DB,Paddy JO,Simmonds P

更新日期：2016-05-01 00:00:00

abstract：:Human parainfluenza virus 3 (HPIV-3) is a leading cause of respiratory disease in children worldwide. Previous sequence analyses of the entire virus genome, among different HPIV-3 strains, demonstrated that HN is the most variable gene. There is a dearth of data on HPIV-3 strains circulating in Saudi Arabia. In this r...

journal_title：Journal of medical virology

authors： Almajhdi FN,Alshaman MS,Amer HM

更新日期：2012-08-01 00:00:00

abstract：:Phylogenetic analysis was performed on hepatitis B virus (HBV) strains obtained from 86 hepatitis B surface antigen (HBsAg) positive donors from Thailand originating throughout the country. Based on the S gene, 87.5% of strains were of genotype C while 10.5% were of genotype B, with all genotype B strains obtained fro...

journal_title：Journal of medical virology

authors： Louisirirotchanakul S,Olinger CM,Arunkaewchaemsri P,Poovorawan Y,Kanoksinsombat C,Thongme C,Sa-Nguanmoo P,Krasae S,Theamboonlert A,Oota S,Fongsatitkul L,Puapairoj C,Promwong C,Weber B

更新日期：2012-10-01 00:00:00

abstract：:BKPyV replication is a risk factor for the development of polyomavirus-associated nephropathy in kidney transplant recipients. Here, the case of a 42 years old Caucasian patient is described who developed a kidney allograft failure because of uncontrolled BKPyV replication 7 months after transplant despite a strong re...

journal_title：Journal of medical virology

authors： Anselmo A,Prezioso C,Saccà FA,Di Lella FM,Palmieri G,Tisone G,Pietropaolo V,Ciotti M

更新日期：2019-09-01 00:00:00

abstract：:Cervical cancer is the second most common cancer among Tunisian women, and the incidence rates vary by region. Three Tunisian registries report age-standardized rates of 6.3/10(5) in the central region, 5.4/10(5) in the north, and 2.7/10(5) in the south. High-risk human papillomavirus (HPV) types and their variants di...

journal_title：Journal of medical virology

authors： KrennHrubec K,Mrad K,Sriha B,Ben Ayed F,Bottalico DM,Ostolaza J,Smith B,Tchaikovska T,Soliman AS,Burk RD

更新日期：2011-04-01 00:00:00

abstract：:Enteroviruses (EVs) are RNA viruses that can cause many clinical syndromes including acute flaccid paralysis (AFP). Within the global polio laboratory network, EVs are categorized either as polioviruses or non-polio enteroviruses (NPEVs). Specific NPEVs have been described in polio-like residual paralytic events in AF...

journal_title：Journal of medical virology

authors： Tushabe P,Howard W,Bwogi J,Birungi M,Eliku JP,Kakooza P,Bukenya H,Namuwulya P,Gaizi J,Tibanagwa M,Kabaliisa T,Mulindwa J,Muhanguzi D,Suchard M,Gumede N,Bakamutumaho B

更新日期：2021-01-17 00:00:00

abstract：:A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg o...

journal_title：Journal of medical virology

authors： Myint S,Harmsen D,Raabe T,Siddell SG

更新日期：1990-06-01 00:00:00

abstract：:A small percentage of renal patients become infected with the BK polyomavirus (BKV), a pathogenic virus that causes BKV-associated nephropathy (BKVN), after kidney transplantation. This study presents a simple, rapid, high-throughput method for BKV genotyping using high-resolution melt analysis (HRMA). Using this nove...

journal_title：Journal of medical virology

authors： Matsuda Y,Qazi Y,Iwaki Y

更新日期：2011-12-01 00:00:00

abstract：:A metagenomic approach based on target independent next-generation sequencing has become a known method for the detection of both known and novel viruses in clinical samples. This study aimed to use the metagenomic sequencing approach to characterize the viral diversity in respiratory samples from patients with respir...

journal_title：Journal of medical virology

authors： Madi N,Al-Nakib W,Mustafa AS,Habibi N

更新日期：2018-03-01 00:00:00

abstract：:A viable virus could not be recovered from a mutant murine cytomegalovirus (MCMV) BAC in which the M34 ORF had been deleted (BACDeltaM34). In contrast, an M34 mutant virus (RcM34), in which the M34 ORF was interrupted by transposon insertion at nt 44,827 of the Smith MCMV BAC, was attenuated in replication both in tis...

journal_title：Journal of medical virology

authors： Baluchova K,Kirby M,Ahasan MM,Sweet C

更新日期：2008-07-01 00:00:00

abstract：:The role of Epstein-Barr virus (EBV) in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) has not been well understood. The aim of the study was to investigate EBV infection and its gene expression in this tumor in order to understand its role in the pathogenesis. EBV infection was scree...

journal_title：Journal of medical virology

authors： Xu WS,Chan AC,Lee JM,Liang RH,Ho FC,Srivastava G

更新日期：1998-12-01 00:00:00

abstract：:This study was designed to determine the prognostic value of erythrocyte adenosine deaminase (ADA) as a possible indicator of progression to AIDS, and compare this with other known cellular and serological markers. At the end of a 3-year study, a cohort of 114 human immunodeficiency virus-1 (HIV-1) seropositive intrav...

journal_title：Journal of medical virology

authors： Casoli C,Lisa A,Magnani G,Starcich R,Fiaccadori F,Bertazzoni U,Zei G

更新日期：1995-02-01 00:00:00

abstract：:An EBV type 1 variant strain was detected in two Turkish siblings (boy and girl), who both suffered and died from similar progressive Epstein-Barr virus (EBV)-associated lymphoproliferative disease. Molecular characterisation of this EBV isolate revealed a 51bp-deletion and six nucleotide changes within the Epstein-Ba...

journal_title：Journal of medical virology

authors： Schuster V,Seidenspinner S,Kreth HW

更新日期：1996-01-01 00:00:00

abstract：:To recognize the period of exaggerated cytokine response in patients with coronavirus disease 2019 (COVID-19) pneumonia, and to describe the clinical outcomes of using tocilizumab as a treatment option. The data of 12 adult COVID-19 pneumonia patients who were followed in the inpatient clinics of Biruni University Med...

journal_title：Journal of medical virology

authors： Borku Uysal B,Ikitimur H,Yavuzer S,Ikitimur B,Uysal H,Islamoglu MS,Ozcan E,Aktepe E,Yavuzer H,Cengiz M

更新日期：2020-11-01 00:00:00

abstract：:In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detecte...

journal_title：Journal of medical virology

authors： Yu Q,Shiramizu B,Nerurkar VR,Hu N,Shikuma CM,Melish ME,Cascio K,Imrie A,Lu Y,Yanagihara R

更新日期：2002-02-01 00:00:00

abstract：:Within the framework of a comprehensive study of the correlation between enteric diseases and wastewater utilization in agricultural settlements (kibbutzim) the prevalence of several viral antibodies was examined among kibbutz residents and overseas volunteers. The latter were assumed to be a group highly susceptible ...

journal_title：Journal of medical virology

authors： Margalith M,Fattal B,Shuval HI,Morag A

更新日期：1986-10-01 00:00:00

abstract：:A previous study revealed a high prevalence of hepatitis B surface antigen (HBsAg) and hepatitis delta virus (HDV) RNA among 249 apparently healthy individuals (mean+/-standard deviation age, 48.4+/-13.9 years; 126 males and 123 females) in Ulaanbaatar, Mongolia. To investigate further the prevalence of HDV infection ...

journal_title：Journal of medical virology

authors： Inoue J,Takahashi M,Nishizawa T,Narantuya L,Sakuma M,Kagawa Y,Shimosegawa T,Okamoto H

更新日期：2005-07-01 00:00:00

abstract：:Individuals infected with HIV have higher circulating Epstein-Barr virus (EBV) DNA load compared to healthy carriers. This study investigated whether level of spontaneous immunoglobulin secreting cells, one of the major hallmarks of HIV infection, is associated with an increase of EBV DNA load in PBMCs and the spontan...

journal_title：Journal of medical virology

authors： Ouedraogo DE,Tuaillon E,Rubbo PA,Bollore K,Foulongne V,Reynes J,Vendrell JP

更新日期：2014-01-01 00:00:00

abstract：:A pathogenic role of precore-defective mutation in the onset of fulminant hepatitis B has been suggested. However, precore-defective mutants do not always cause fulminant hepatitis B and are not always isolated from affected patients. These findings strongly suggest the presence of some additional important mutations ...

journal_title：Journal of medical virology

authors： Kaneko M,Uchida T,Moriyama M,Arakawa Y,Shikata T,Gotoh K,Mima S

更新日期：1995-11-01 00:00:00

abstract：:To provide background for future hepatitis A vaccine trials, sera were collected from 0- to 4-year-old Liberian infants and their mothers on two occasions an average of 14.75 months apart and tested for antibody to hepatitis A virus (anti-HAV). The prevalence of anti-HAV rose from 2.5% in infants 0-6 months of age to ...

journal_title：Journal of medical virology

authors： Prince AM,Brotman B,Richardson L,White T,Pollock N,Riddle J

更新日期：1985-04-01 00:00:00

abstract：:Cervical specimens collected from 163 cytologically healthy women were screened for the presence of human papillomavirus (HPV) DNA and anti-HPV secretory IgA antibodies. HPV DNA was detected by a general primer mediated polymerase chain reaction (PCR), which amplifies a conserved region from the L1 ORF of genital HPVs...

journal_title：Journal of medical virology

authors： Veress G,Kónya J,Csiky-Mészáros T,Czeglédy J,Gergely L

更新日期：1994-06-01 00:00:00