Abstract：
:High-conductance calcium-activated potassium (maxi-K) channels comprise a specialized family of K+ channels. They are unique in their dual requirement for depolarization and Ca2+ binding for transition to the open, or conducting, state. Ion conduction through maxi-K channels is blocked by a family of venom-derived peptides, such as charybdotoxin and iberiotoxin. These peptides have been used to study function and structure of maxi-K channels, to identify novel channel modulators, and to follow the purification of functional maxi-K channels from smooth muscle. The channel consists of two dissimilar subunits, alpha and beta. The alpha subunit is a member of the slo Ca(2+)-activated K+ channel gene family and forms the ion conduction pore. The beta subunit is a structurally unique, membrane-spanning protein that contributes to channel gating and pharmacology. Potent, selective maxi-K channel effectors (both agonists and blockers) of low molecular weight have been identified from natural product sources. These agents, together with peptidyl inhibitors and site-directed antibodies raised against alpha and beta subunit sequences, can be used to anatomically map maxi-K channel expression, and to study the physiologic role of maxi-K channels in various tissues. One goal of such investigations is to determine whether maxi-K channels represent novel therapeutic targets.
journal_name
J Bioenerg Biomembrjournal_title
Journal of bioenergetics and biomembranesauthors
Kaczorowski GJ,Knaus HG,Leonard RJ,McManus OB,Garcia MLdoi
10.1007/BF02110699subject
Has Abstractpub_date
1996-06-01 00:00:00pages
255-67issue
3eissn
0145-479Xissn
1573-6881journal_volume
28pub_type
杂志文章,评审abstract：:The fungal plasma membrane contains a proton-translocating ATPase that is closely related, both structurally and functionally, to the [Na+, K+]-, [H+, K+]-, and [Ca2+]-ATPases of animal cells, the plasma-membrane [H+]-ATPase of higher plants, and several bacterial cation-transporting ATPases. This review summarizes cu...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00808117
更新日期：1989-10-01 00:00:00
abstract：:Three classes of inner mitochondrial membrane (IMM) channel activities have been defined by direct measurement of conductance levels in membranes with patch clamp techniques in 150 mM KCl. The "107 pS activity" is slightly anion selective and voltage dependent (open with matrix positive potentials). "Multiple conducta...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00769536
更新日期：1992-02-01 00:00:00
abstract：:Members of the genus Methanosarcina are strictly anaerobic archaea that derive their metabolic energy from the conversion of a restricted number of substrates to methane. H2 + CO2 are converted to CH4 via the CO2-reducing pathway, while methanol and methylamines are metabolized by the methylotrophic pathway. Two novel...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/b:jobb.0000019598.64642.97
更新日期：2004-02-01 00:00:00
abstract：:The purification of the channel-forming component of the mitochondrial calcium uniporter and its channel properties are described. After ethanol and 50% ethanol-water extraction of mitochondria from beef heart or perfused rat liver, the extract was passed through thiopropyl-Sepharose 6B column, and absorbed components...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00763072
更新日期：1994-04-01 00:00:00
abstract：:The hexosamine biosynthetic pathway (HBP) integrates glucose, amino acids, fatty acids and nucleotides metabolisms for uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) synthesis. UDP-GlcNAc is the nucleotide sugar donor for O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) processes. O-GlcNAc transferase (OGT...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-018-9747-y
更新日期：2018-06-01 00:00:00
abstract：:Defects in Complex I of the mitochondrial respiratory chain have been identified in 38 patients. The clinical and laboratory features are reviewed and the results of recently devised strategies aimed at characterizing the primary molecular and genetic abnormalities are presented. Although not exhaustive, these studies...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00769638
更新日期：1988-06-01 00:00:00
abstract：:A study of NADH ferricyanide reductase activity in oriented vesicles or open ghosts of human and porcine erythrocytes shows that the dehydrogenase activity can have three types of orientation in the membrane. There is activity which responds only to acceptors and NADH exclusively on the inside face, or exclusively on ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743068
更新日期：1982-12-01 00:00:00
abstract：:Transformation of 3T3 cells by SV40 virus changes the properties of the transplasma membrane electron transport activity which can be assayed by reduction of external ferric salts. After 42 h of culture and before the growth rate is maximum, the transformed cells have a much slower rate of ferric reduction. The change...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00786008
更新日期：1991-12-01 00:00:00
abstract：:Evidence suggests membrane bound F(1)F(0)-ATPase complexes form stable associations such that dimers can be retrieved from detergent lysates of mitochondria isolated from a range of sources including algae, higher plants, yeast and bovine heart, and plant chloroplasts. The physiological relevance of these interactions...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-005-4128-8
更新日期：2005-04-01 00:00:00
abstract：:The role of the adenine nucleotide translocase on Ca2+ homeostasis in mitochondria from brown adipose tissue was examined. It was found that in mitochondria incubated with 50 microM Ca2+, ADP was not needed to retain the cation, but it was required for strengthening the inhibitory effect of cyclosporin on membrane per...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Cytoplasmic male sterility arises when mitochondrial activities are disrupted that are essential for pollen development. Rearrangements in the mitochondrial genome that create novel open reading frames are strongly correlated with CMS phenotypes in a number of systems. The morphological aberrations which indicate CMS-...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF02110007
更新日期：1995-08-01 00:00:00
abstract：:The ATP synthase of the yeast Saccharomyces cerevisiae is composed of 20 different subunits whose primary structure is known. The organization of proteins that constitute the membranous domain is now under investigation. Cysteine insertions combined with the use of nonpermeant maleimide reagents and cross-linking reag...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1005580020547
更新日期：2000-08-01 00:00:00
abstract：:A rapid loss of accumulated Ca2+ is produced by addition of H+ to isolated heart mitochondria. The H+-dependent Ca+ efflux requires that either (a) the NAD(P)H pool of the mitochondrion be oxidized, or (b) the endogenous adenine nucleotides be depleted. The loss of Ca2+ is accompanied by swelling and loss of endogenou...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00743069
更新日期：1982-12-01 00:00:00
abstract：:As the terminal component of the mitochondrial respiratory chain, cytochrome c oxidase plays a vital role in cellular energy transformation. Human cytochrome c oxidase is composed of 13 subunits. The three major subunits form the catalytic core and are encoded by mitochondrial DNA (mtDNA). The remaining subunits are n...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1022638013825
更新日期：1997-04-01 00:00:00
abstract：:The induction of millisecond delayed fluorescence mediated by PS I-dependent proton pumping has been used as an indicator of the time course with which those protons equilibrate with sites on the oxygen-evolving enzyme complex (Bowes, J. M., and Crofts, A. R. (1978). Z. Naturforsch. 33C, 271-275). We found that the in...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/BF00769732
更新日期：1987-02-01 00:00:00
abstract：:We report on the influence of dielectric pulse injection on the energy metabolism of yeast cells with a planar interdigitated electrode interface. The energy metabolism was measured via NADH fluorescence. The application of dielectric pulses results in a distinct decrease of the fluorescence, indicating a response of ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-006-9014-5
更新日期：2006-04-01 00:00:00
abstract：:The midgut plasma membrane V-ATPase from larval Manduca sexta, the tobacco hornworm, is the sole energizer of any epithelial ion transport in this tissue and is responsible for the alkalinization of the gut lumen up to a pH of more than 11. This mini-review deals with those topics of research on this enzyme which may ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/a:1005448614450
更新日期：1999-02-01 00:00:00
abstract：:We investigated the effects of K(ATP) channel openers diazoxide and pinacidil on the respiration rate and membrane potential (deltapsi) of rat heart mitochondria, oxidizing pyruvate and malate. Diazoxide and pinacidil (58.8-1348.3 microM) increased the V2 (-ADP) respiration rate accordingly by 13-208% and 30-273% and ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1013870704002
更新日期：2002-02-01 00:00:00
abstract：:Previous in vitro studies indicated that mutation of both K234 and K236 to arginine, glutamine, or glutamic acid impaired the ability of the voltage-dependent anion channel (VDAC1) to insert into the outer membrane of the mitochondria (Smith et al. 1995). These same mutants were expressed in a strain of Saccharomyces ...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1005451811802
更新日期：1999-04-01 00:00:00
abstract：:This study was designed to investigate the energetics of isolated rat hepatocyte swelling due to sodium-cotransported amino acid accumulation in a medium containing either glucose or octanoate as basal substrate. We show that the size of the increase in cytosolic volume is directly correlated with the total amino acid...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1022487119390
更新日期：1997-12-01 00:00:00
abstract：:NYGGF4 (also called PID1) is a recently discovered gene that is involved in obesity-related insulin resistance (IR). We aimed in the present study to further elucidate the effects of NYGGF4 on IR and the underlying mechanisms through using metformin treatment in 3T3-L1 adipocytes. Our data showed that the metformin pr...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-012-9472-x
更新日期：2012-12-01 00:00:00
abstract：:In Propionigenium modestum, ATP is manufactured from ADP and phosphate by the enzyme ATP synthase using the free energy of an electrochemical gradient of Na+ ions. The P. modestum ATP synthase is a clear member of the family of F-type ATP synthases and the only major distinction is an extension of the coupling ion spe...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1005608823087
更新日期：2000-10-01 00:00:00
abstract：:The past 5 years has witnessed an advance in our understanding of alpha1G (Cav3.1), alpha1H (Cav3.2), and alpha11 (Car3.3), the pore-forming subunits of T-type or low-voltage-activated calcium channels (LVAs). LVAs differ in their localization and molecular, biophysical, and biochemical properties, but all conduct a t...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1023/b:jobb.0000008024.77488.48
更新日期：2003-12-01 00:00:00
abstract：:Caloric restriction (CR) is widely known to increase life span and resistance to different types of injuries in several organisms. We have previously shown that mitochondria from livers or brains of CR animals exhibit higher calcium uptake rates and lower sensitivity to calcium-induced mitochondrial permeability trans...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-020-09838-4
更新日期：2020-08-01 00:00:00
abstract：:Advanced glycation end products (AGEs) and methylglyoxal (MG), an important intermediate in AGEs synthesis, are thought to contribute to protein aging and to the pathogenesis of age-and diabetes-associated complications. This study was intended to investigate brain mitochondria bioenergetics and oxidative status of ra...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-014-9551-2
更新日期：2014-10-01 00:00:00
abstract：:Yeasts capable of growing and surviving at high temperatures are regarded as thermotolerant. For appropriate functioning of cellular processes and cell survival, the maintenance of an optimal redox state is critical of reducing and oxidizing species. We studied mitochondrial functions of the thermotolerant Kluyveromyc...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-017-9696-x
更新日期：2017-04-01 00:00:00
abstract：:Proton transport-coupled unisite catalysis was measured with the H(+)-ATPase from chloroplasts. The reaction was measured in the ATP hydrolysis direction under deenergized conditions and in the ATP synthesis direction under energized conditions. The equilibrium constant of the enzyme does not change upon energization,...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF00762367
更新日期：1992-10-01 00:00:00
abstract：:Hepatic iron toxicity because of iron overload seems to be mediated by lipid peroxidation of biological membranes and the associated organelle dysfunctions. However, the basic mechanisms underlying this process in vivo are still little understood. Gerbils were dosed with weekly injections of iron-dextran alone or in c...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1023/a:1005512014280
更新日期：2000-04-01 00:00:00
abstract：:The small alkylating molecule, 3-bromopyruvate (3BP), is a potent and specific anticancer agent. 3BP is different in its action from most currently available chemo-drugs. Thus, 3BP targets cancer cells' energy metabolism, both its high glycolysis ("Warburg Effect") and mitochondrial oxidative phosphorylation. This inh...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章
doi：10.1007/s10863-012-9417-4
更新日期：2012-02-01 00:00:00
abstract：:Gap junction channels have long been viewed as static structures containing a large-diameter, aqueous pore. This pore has a high permeability to hydrophilic molecules of approximately 900 daltons in molecular weight and a weak ionic selectivity. The evidence leading to these conclusions is reviewed in the context of m...
journal_title：Journal of bioenergetics and biomembranes
pub_type： 杂志文章,评审
doi：10.1007/BF02110109
更新日期：1996-08-01 00:00:00
