Abstract:
:Experimentation has been carried out to study proliferation of plasma cells in the chicken Harderian gland (HG) and to determine if a HG factor influences immune cell (i.e., B cell) proliferation. In young chickens, flow cytometric analysis of propidium iodide (PI)-stained plasma cells revealed that the percentages of cells in both the synthetic (S) and mitotic (G2M) phases of the cell cycle were highest between 6 and 9 weeks of age. A pattern of plasma cell depletion and repopulation in the HG was observed following administration of emetine dihydrochloride. At 3 and 5 days posttreatment the plasma cell population decreased, and by 7 days posttreatment repopulation of the gland with plasma cells occurred. This repopulation appeared as a result of plasma cell proliferation within the HG. Anti-5-bromo-2'-deoxyuridine (BrdUrd) staining of frozen sections showed that the numbers of plasma cells incorporating BrdUrd were low at 3 days posttreatment but were as high, or higher than, controls at 5 and 7 days posttreatment. These results were verified with flow cytometric data of PI-stained plasma cells. Data from bursal cell bioassays revealed proliferative activity influenced by a HG factor. Coculture of bursal cells with phorbol dibutyrate and diluted HG supernatants resulted in prolonged and increased proliferation of these cells. It is possible that the HG of chickens supports plasma cell proliferation through the elaboration of a factor which acts like a lymphokine.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Scott TR,Savage MLdoi
10.1002/(SICI)1097-0029(19960601)34:2<149::AID-JEMsubject
Has Abstractpub_date
1996-06-01 00:00:00pages
149-55issue
2eissn
1059-910Xissn
1097-0029pii
10.1002/(SICI)1097-0029(19960601)34:2<149::AID-JEMjournal_volume
34pub_type
杂志文章abstract::Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so-attached particle can in turn act as a probing tool to study particle-substrate and particle-particle interactions. Attachment of the fluorescent particle...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20816
更新日期:2010-08-01 00:00:00
abstract::We have used high voltage electron microscopy and computer-generated three-dimensional reconstructions from serial sections to elucidate the structure of taste bud cells and their associated synapses in fungiform taste buds of the mouse. Five fungiform taste buds (two of which were serially sectioned) were examined wi...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070260207
更新日期:1993-10-01 00:00:00
abstract::Locusts are attractive model preparations for cellular investigations of neurodevelopment. In this study, we investigate the immunocytochemical localization of histamine in the developing ventral nerve cord of two locust species, Schistocerca gregaria and Locusta migratoria. Histamine is the fast neurotransmitter of p...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20980
更新日期:2011-10-01 00:00:00
abstract::Growth-year authentication has extraordinary significance for plant growth, structure and development research, and has a wide range of applications in value assessment of economic crops. Panax ginseng is the most commonly used medicinal plant in Asian countries. The fix number of growth-year is an important quality e...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22610
更新日期:2016-02-01 00:00:00
abstract::Phytoliths are an important component for interpreting the ancient botanical record. However, phytoliths can be altered through heating, either as the result of such activities as firing ceramics, clay molds use for casting metal or in hearths. Phytoliths can also be altered through heating as the result of creating c...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22004
更新日期:2012-07-01 00:00:00
abstract::Fluorescence correlation spectroscopy (FCS) is a very sensitive technique that can be used, e.g., for the measurement of low concentrations and for the investigation of transport of fluorescent molecules. Fluorescence lifetime imaging (FLIM) provides spatially resolved information about molecular fluorescence lifetime...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20428
更新日期:2007-05-01 00:00:00
abstract::Among the various corrosion protection strategies for structural steels, coating techniques provide the most cost-effective protection and have been used as the primary mode of corrosion protection. Existing coating techniques however have been used mainly for their barrier capability and therefore all have a limited ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22283
更新日期:2013-11-01 00:00:00
abstract::The preparation of optically clear, thick sections of fragile embryonic tissues greatly aids the power of confocal scanning laser microscopy in imaging three-dimensional structures. We report here conditions for embedding, sectioning, and staining embryos in polyacrylamide gels for a variety of confocal imaging techni...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070300608
更新日期:1995-04-15 00:00:00
abstract::The anterior adhesive areas of a monocotylid monogenean, Merizocotyle australensis (Merizocotylinae), were investigated. They comprise 6 ventral apertures in 2 groups of 3 arranged at the anterolateral margins of the head. These regions are also well supplied with groups of cilia. Each aperture is 13.8 to 15.8 microm ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980801)42:3<200::AID-JEM
更新日期:1998-08-01 00:00:00
abstract::Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290404
更新日期:1994-11-01 00:00:00
abstract::In certain cases of infertility in domestic species, and in the homozygous c-ros tyrosine kinase knockout mouse, males are infertile as a result of unopposed sperm swelling. This induces flagellar angulation, preventing their normal migration in the female tract. Angulation always occurs at the site of the cytoplasmic...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10314
更新日期:2003-05-01 00:00:00
abstract::The photoreceptor population in Xenopus consists of a green-sensitive rod (lambda(max) = 523 nm), a blue-sensitive rod (lambda(max) = 445 nm) and three classes of cone. The largest cone is red-sensitive (lambda(max) = 611 nm). The intermediate cone is presumed to be blue-sensitive based on physiological criteria, wher...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000901)50:5<338::AID-JEMT3>3.0
更新日期:2000-09-01 00:00:00
abstract::Voltage-gated sodium channels are largely localized to the nodes of Ranvier in myelinated axons, providing the physiological basis for saltatory conduction. Studies using antisodium channel antibodies have shown that along demyelinated axons sodium channels form new distributions. The nature of this changed distributi...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19960801)34:5<445::AID-JEM
更新日期:1996-08-01 00:00:00
abstract::We compared Hall's peak to continuum ratio method with a peak ratio method in order to quantify light elements (C, N, and O) in organic specimens as a model for biological thin sections. X-ray spectra were recorded by an energy dispersive X-ray spectrometer equipped with an ultra thin window detector. Spectra were pro...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20010115)52:2<231::AID-JEMT1008>
更新日期:2001-01-15 00:00:00
abstract::Studies on embryonic development in vitro as well as observations in vivo, suggested that two aspects of oviduct physiology are important for early development. On one side has to be considered the oviduct "environment": temperature, pH, osmotic pressure, nutrients, oxygen tension, free radical scavengers, etc. On the...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070320102
更新日期:1995-09-01 00:00:00
abstract::Several studies support the role of Western-style diet (WD) in inflammatory bowel disease (IBD). Toll-like receptors/NOD-like receptors (TLRs/NLRs) are important to maintain a healthy epithelium as well as inducing inflammation. Given that dietary factors influence IBD development, that epithelial dysfunction is thoug...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22831
更新日期:2017-06-01 00:00:00
abstract::Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA-protein complex. Visualizing the mRNA is crucial for unde...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23214
更新日期:2019-06-01 00:00:00
abstract::The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 1...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23155
更新日期:2019-02-01 00:00:00
abstract::After more than two centuries of almost sporadic inquiry as to the existence and function of the human vomeronasal system (VNS), the last decade has seen a resurgent interest in it. The principal question vexing many laboratories is whether adult humans retain the VNS that clearly develops during fetal growth. Additio...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980615)41:6<530::AID-JEM
更新日期:1998-06-15 00:00:00
abstract::Pituitary-derived factors, including ACTH, have been widely implicated in initiating adrenal regeneration. However, recent work has demonstrated that adrenal regeneration is also modulated by adrenal nerves that extensively reinnervate the regenerating adrenal. Moreover, transection of the splanchnic nerve removes sen...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10334
更新日期:2003-06-15 00:00:00
abstract::The autonomic neuromuscular junction at a varicosity in the vas deferens is defined by the localization of the vesicle-associated protein syntaxin in high concentrations in the axolemma and a high density of P2x1 receptors in a cluster beneath the varicosity. Calcium fluxes have been observed in all individual varicos...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980915)42:6<433::AID-JEM
更新日期:1998-09-15 00:00:00
abstract::Understanding the functional morphology and mobility of appendages of fossil animals is important for exploring ecological traits such as feeding and locomotion. Previous work on fossils from the 518 million-year-old Chengjiang biota of China was based mainly on two-dimensional information captured from the surface of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23628
更新日期:2020-11-06 00:00:00
abstract::STM tips made from antiferromagnetic MnNi have been used to investigate the atomic structure of the (001) and (111) surfaces of Fe3O4. The clean (001) surface displays a ( radical2 x radical2)R45 degrees superlattice, which is attributed to charge-ordering on the surface, where Fe(2+)-Fe2+ and Fe(3+)-Fe3+ dimers can b...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20148
更新日期:2005-02-01 00:00:00
abstract::The intravacuolar network inside the parasitophorous vacuole of Toxoplasma gondii consists of an intricate system of membrane-limited tubules of uncertain role in parasite development. We propose that it is an important structural support to the maintenance of the parasites in the characteristic rosette arrangement of...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20182
更新日期:2005-05-01 00:00:00
abstract::Regeneration of skeletal parts of fossil echinoderms is reviewed. Among regenerations of various fossil echinoderms, those of crinoids are most common and many examples have been reported. Mesozoic and Cenozoic crinoids, the subclass Articulata, are characterized by the dominance of muscular articulations in the arms ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1186
更新日期:2001-12-15 00:00:00
abstract::We have summarized current knowledge on the aging of the immune system in three vertebrate groups: fish, amphibians and birds. Available data are few due to difficulties in studying ageing in natural populations and in accurately determining age. In all vertebrates, the most obvious evidence of the senescence of lymph...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10409
更新日期:2003-12-15 00:00:00
abstract::The ultrastructural characteristics of peptidergic peripheral contacts in the snail, Helix pomatia, were investigated, with special attention to the innervation of the heart, buccal mass, and salivary gland by Mytilus inhibitory peptide-immunoreactive neurons. Following the application of correlative light- and electr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/1097-0029(20000615)49:6<534::AID-JEMT4>3.0
更新日期:2000-06-15 00:00:00
abstract::Transgenic mice are valuable experimental models of human endocrine diseases. Targeted ablation of specific cell lineages or insertion of genes coding for releasing factors, hormones, growth factors, and oncogenes fused with appropriate promoters, or mutated genes, can induce several pituitary disorders. Various hypos...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19971015)39:2<194::AID-JEM
更新日期:1997-10-15 00:00:00
abstract::Detailed maps of the A-T distribution within human mitotic chromosomes labeled with BrdU are obtained with a high resolution scanning ion microprobe through the detection of bromine by imaging secondary ion mass spectrometry (SIMS). Corresponding maps of the emission loci of the molecular ion CN- describe the overall ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970215)36:4<301::AID-JEM
更新日期:1997-02-15 00:00:00
abstract::The adaptations of the mouse uterus to pregnancy include extensive modifications of the cells and extracellular matrix of the endometrial connective tissue that surround the embryos. Around each implanted embryo this tissue redifferentiates into a transient structure called decidua, which is formed by polygonal cells ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20265
更新日期:2006-01-01 00:00:00