Abstract:
:Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so-attached particle can in turn act as a probing tool to study particle-substrate and particle-particle interactions. Attachment of the fluorescent particle occurs at the apical region of an atomic force microscope (AFM) cantilever tip and it endures repetitive loading-unloading cycles against the sample surface. Fluorescence microscopy has been used to address the exact location of the attached particle in the cantilever and to identify the moment when the particle contacts the sample. Moreover, we have observed that fluorescence intensity at the contact point is lower when the probing particle contacts another fluorescent particle than when it contacts the nonfluorescent substrate. The change in fluorescence is attributed to local changes of pH and interparticle-quenching of fluorophores in the contact region. These findings are promising since they constitute a chemical-free way to attach bioparticles to AFM probes under physiological conditions. The atomic force microscopy combined with fluorescence microscopy provides a straight forward method to study particle/particle and particle/substrate interactions, as well as to investigate mechanical properties of biocolloids.
journal_name
Microsc Res Techjournal_title
Microscopy research and techniqueauthors
Moreno-Flores S,Georgieva R,Xiong Y,Melzak K,Bäumler H,Luis Toca-Herrera Jdoi
10.1002/jemt.20816subject
Has Abstractpub_date
2010-08-01 00:00:00pages
746-51issue
8eissn
1059-910Xissn
1097-0029journal_volume
73pub_type
杂志文章abstract::Polycrystalline silver catalysts are used extensively for the partial oxidation of methanol to formaldehyde, which is then primarily incorporated in the synthesis process for adhesives and resins. In order to maximize formaldehyde production it is essential to gain a comprehensive understanding of the complex microstr...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19970301)36:5<382::AID-JEM
更新日期:1997-03-01 00:00:00
abstract::Virus particles and viral inclusions were detected by transmission electron microscopy examination of sections of the seminal vesicles and mucus gland of asymptomatic young drones from colonies of Apis mellifera lightly infested by Varroa mite. In the mucus gland the infection was found in the muscular sheath and epit...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22024
更新日期:2012-07-01 00:00:00
abstract::An energy-filtering transmission electron microscope with 300 kV acceleration voltage was developed and the spatial resolution of elemental distribution images was improved. Observing oxygen monolayers in Al(11)O(3)N(9), it was shown that the actual resolution attained is up to 0.5 nm. Surface plasmon loss images of s...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20025
更新日期:2004-02-15 00:00:00
abstract::After more than two centuries of almost sporadic inquiry as to the existence and function of the human vomeronasal system (VNS), the last decade has seen a resurgent interest in it. The principal question vexing many laboratories is whether adult humans retain the VNS that clearly develops during fetal growth. Additio...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980615)41:6<530::AID-JEM
更新日期:1998-06-15 00:00:00
abstract::The mammary adenocarcinoma cell line MTLn3 is chemotactic towards epidermal growth factor (EGF), and this induced motility is thought to promote breast cancer invasion and metastasis. Stimulation of MTLn3 cells with EGF results in the extension of a flat, thin structure filled with filamentous actin and termed a lamel...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19981201)43:5<433::AID-JEM
更新日期:1998-12-01 00:00:00
abstract::On continuing our studies on head and neck neoplasia, specimens from salivary gland tumors have been explored by using infrared microimaging spectroscopy to discern healthy from neoplastic tissues. Samples with Warthin tumor, epithelial displasia, marginal B-cell lymphoma, low-grade adenocarcinoma, and adenoid cystic ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20644
更新日期:2009-02-01 00:00:00
abstract::This study investigated the relationship between enteroendocrine and mucus-secreting cells distribution, the severity of colonic mucosal injury and intestinal motility in experimental colorectal carcinogenesis. Using a standardized murine model of colorectal carcinogenesis, eight-weeks-old female Wistar rats weighting...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22534
更新日期:2016-01-01 00:00:00
abstract::This study was conducted to provide comprehensive information on the anatomical and histological features of the upper (UL) and lower (LL) lips of Rahmani sheep by gross examination, morphometric analysis in addition to Light and scanning electron microscope (SEM). Samples from normal healthy adult male sheep heads we...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23660
更新日期:2020-12-01 00:00:00
abstract::Protein expression patterns are a primary determinant of tissue function and in this study we developed methods to study protein expression over macroscopic distances at subcellular levels of detail. Using the mammalian lens as a model tissue system, we show that by combining two-photon microscopy with novel image mon...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10360
更新日期:2003-09-01 00:00:00
abstract::In high throughput microscopy, it is often assumed that the objects under investigation are fixed spatially. In addition, it is also presumed that the objects are sufficiently populated, otherwise there will be need to search through vast tracks of field of views before any recording can be done. The ability to collec...
journal_title:Microscopy research and technique
pub_type: 信件
doi:10.1002/jemt.22238
更新日期:2013-08-01 00:00:00
abstract::The apolipoprotein E (apoE) epsilon4 allele increases risk of Alzheimer's disease (AD), perhaps by accelerating plaque formation, or by impairing neuron repair. Considerable evidence supports both mechanisms. AD patients with epsilon4 have more and earlier amyloid deposits than do patients without epsilon4. The same i...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/1097-0029(20000815)50:4<278::AID-JEMT5>3.0
更新日期:2000-08-15 00:00:00
abstract::A synchrotron radiation fiber diffraction structural study of the axial filament of siliceous spicules from two species of marine sponges (the Demosponge Geodia cydonium and the Hexactinellid Scolymastra joubini) was carried out. The sharpness of the spots in the diffraction patterns indicated that the protein units i...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10403
更新日期:2003-11-01 00:00:00
abstract::Ascidian embryos are useful for examining how events that occur during fertilization and cleavage affect gastrulation because they gastrulate early in development, during the seventh cleavage. In ascidians, both dorsal-ventral and anterior-posterior axes are determined before first cleavage. The dorsal-ventral axis is...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1070260403
更新日期:1993-11-01 00:00:00
abstract::This paper summarizes the essential features of the various facilities dedicated to in situ irradiation effects research around the world at the present time, mentions some essential techniques that are involved in this type of research, and describes very briefly some examples of studies employing these facilities an...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19980915)42:4<255::AID-JEM
更新日期:1998-08-15 00:00:00
abstract::Ketogenic diets (KDs) have shown beneficial effects in experimental models of neurodegeneration, designating aged individuals as possible recipients. However, few studies have investigated their consequences on aging brain. Here, late-adult rats (19 months of age) were fed for 8 weeks with two medium chain triglycerid...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20737
更新日期:2009-12-01 00:00:00
abstract::Peroxisomes are essential organelles that may be involved in various functions, dependent on organism, cell type, developmental stage of the cell, and the environment. Until recently, peroxisomes were viewed as a class of static organelles that developed by growth and fission from pre-existing organelles. Recent obser...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.10323
更新日期:2003-06-01 00:00:00
abstract::This review examines the morphology of the adrenal gland with particular reference to the adrenal vasculature. It examines the possibility that variability in adrenal gland responsiveness may be attributable to neural or hormonal modulation of adrenal blood flow. Changes in the rate of blood flow through the adrenal c...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19970315)36:6<546::AID-JEM
更新日期:1997-03-15 00:00:00
abstract::It is known that, besides a wide range of functions, melatonin provides protection against oxidative stress, thanks to its ability to act, directly, as a free radical scavenger and, indirectly, by stimulating antioxidant enzymes production and mitochondrial electron transport chain efficiency. Oxidative stress is one ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22663
更新日期:2016-06-01 00:00:00
abstract::Nowadays the processes of crossing of different mammalian species are well characterized in light of genetics, and possibility of reproduction. Generally, lack detailed studies on changes in anatomy and histology of internal systems of hybrids. Bison bonasus hybrid is an interspecific species between the bull of Europ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23516
更新日期:2020-10-01 00:00:00
abstract::The present study focused to elucidate the promising role of probiotics in the fish culture, by employing microscopic and biochemical parameters to rationalize the possible outcomes. After preliminary feeding trial histopathological analysis revealed clear and visible changes in the intestinal and liver cells of CTRL ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.23416
更新日期:2020-03-01 00:00:00
abstract::Studies in insect gustation have a long history in general physiology, particularly with work on fly labellar and tarsal sensilla and in the general field of insect-plant interactions, where work on immature Lepidoptera and chrysomelid beetles has been prominent. Much more emphasis has been placed on the physiological...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(19991215)47:6<401::AID-JEM
更新日期:1999-12-15 00:00:00
abstract::The glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicul...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22040
更新日期:2012-08-01 00:00:00
abstract::Term and preterm cervical ripening and dilatation have similarities with an inflammatory reaction. Since cell adhesion molecules are involved in this process, investigations on the expression of intercellular adhesion molecule-1, endothelial leukocyte adhesion molecule-1, vascular cell adhesion molecule-1, and platele...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.10281
更新日期:2003-03-01 00:00:00
abstract::Ion transport mediated by osmotically induced chloride channels in HT29 cells was investigated by X-ray microanalysis of cell cultures. The cells were cultured on cellulose nitrate filters, and exposed to isotonic, hypotonic, or hypertonic solutions. Then the NaCl-rich medium was removed by rinsing the cells with a wa...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0029(19980101)40:1<72::AID-JEMT
更新日期:1998-01-01 00:00:00
abstract::Changes in the intracellular Ca2+ concentration ([Ca2+]i) within CA1 hippocampal pyramidal neurons were imaged using confocal laser scanning microscopy in conjunction with Ca(2+)-sensitive fluorescent indicators. The imaging was performed in thick hippocampal brain slices while simultaneously measuring or controlling ...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070290404
更新日期:1994-11-01 00:00:00
abstract::Uranium-contaminated soils from the U.S. Department of Energy (DOE) Fernald Site, Ohio, have been examined by a combination of backscattered electron imaging (BSE) and analytical electron microscopy with electron diffraction (AEM). The inhomogeneous distribution of particulate uranium phases in the soil required the d...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.1070310208
更新日期:1995-06-01 00:00:00
abstract::The small GTPase Rho, which regulates cell shape, is thought to contribute to cytokinesis. Recently, Citron was characterized as a Rho target. This large protein contains a Ser/Thr kinase domain related to that of ROCK, another Rho effector. Both endogenous Citron and recombinant Citron localize to the cleavage furrow...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1097-0029(20000415)49:2<123::AID-JEM
更新日期:2000-04-15 00:00:00
abstract::Regeneration of skeletal parts of fossil echinoderms is reviewed. Among regenerations of various fossil echinoderms, those of crinoids are most common and many examples have been reported. Mesozoic and Cenozoic crinoids, the subclass Articulata, are characterized by the dominance of muscular articulations in the arms ...
journal_title:Microscopy research and technique
pub_type: 杂志文章,评审
doi:10.1002/jemt.1186
更新日期:2001-12-15 00:00:00
abstract::The nasal fluid is an easily accessible form of airway surface liquid. The objective of this study was to find a technically easy and reproducible method for sampling and analysis of this fluid. In a pilot study, several methods to carry out X-ray microanalysis of sub-microliter droplets were compared. Acceptable resu...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.20020
更新日期:2004-02-01 00:00:00
abstract::Phytoliths are an important component for interpreting the ancient botanical record. However, phytoliths can be altered through heating, either as the result of such activities as firing ceramics, clay molds use for casting metal or in hearths. Phytoliths can also be altered through heating as the result of creating c...
journal_title:Microscopy research and technique
pub_type: 杂志文章
doi:10.1002/jemt.22004
更新日期:2012-07-01 00:00:00