Citron, a Rho target that affects contractility during cytokinesis.

Abstract:

:The small GTPase Rho, which regulates cell shape, is thought to contribute to cytokinesis. Recently, Citron was characterized as a Rho target. This large protein contains a Ser/Thr kinase domain related to that of ROCK, another Rho effector. Both endogenous Citron and recombinant Citron localize to the cleavage furrow in dividing cells and to the midbody in post-mitotic cells. Moreover, overexpression of Citron deleted from its C-terminal sequence caused abnormal contractions specifically during cytokinesis, resulting in the formation of multinucleated cells. Cell shape, F-actin, intermediate filaments, and microtubules appeared essentially normal in these cells during interphase. Thus, Citron is a Rho effector that appears to function during cytokinesis, modulating its contractile process. In brain, however, Citron is highly expressed in a subset of neurons as a brain-specific isoform that lacks a kinase domain, Citron-N. This protein accumulates in synapses and associates to the NMDA receptor via interaction with the adaptor protein PSD95, suggesting that the function of Citron is specialized in the neurons.

journal_name

Microsc Res Tech

authors

Madaule P,Furuyashiki T,Eda M,Bito H,Ishizaki T,Narumiya S

doi

10.1002/(SICI)1097-0029(20000415)49:2<123::AID-JEM

keywords:

subject

Has Abstract

pub_date

2000-04-15 00:00:00

pages

123-6

issue

2

eissn

1059-910X

issn

1097-0029

pii

10.1002/(SICI)1097-0029(20000415)49:2<123::AID-JEM

journal_volume

49

pub_type

杂志文章,评审
  • Microvasculature of the bovine claw demonstrated by improved micro-corrosion-casting technique.

    abstract::A new and improved technique for microvascular corrosion casting was developed and verified by examination of corrosion casts of 90 bovine limbs. The described technique renders a complete filling of the vasculature of the claw even in regions that hitherto proved to be difficult regarding completeness of filling such...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19990501)45:3<184::AID-JEM

    authors: Hirschberg RM,Mülling CK,Bragulla H

    更新日期:1999-05-01 00:00:00

  • Diffracted-light contrast enhancement: a re-examination of oblique illumination.

    abstract::A re-examination and modification of the very old oblique illumination technique has resulted in a method for contrast enhancement in microscopes, diffracted-light contrast (DLC), which provides high-contrast, high-resolution images of unstained biological material. The technique, which utilizes the diffracted light f...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0029(19990815/01)46:4/5<334::AI

    authors: Piekos WB

    更新日期:1999-08-15 00:00:00

  • Unique dentition of lungfish.

    abstract::Lungfish are a group of ancient fish, represented almost continuously in the fossil record from their first appearance in the Devonian to the present time. They have numerous unique characters in the dentition, found only among lungfish, as well as some that are shared with other groups of fish and with higher vertebr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10221

    authors: Kemp A

    更新日期:2002-12-01 00:00:00

  • Laminins and their roles in mammals.

    abstract::Laminins are alpha-beta-gamma heterotrimeric components of all basement membranes. Laminins are now known to play the central role in organizing and establishing the basement membrane. The diversity of laminins allows them to impart special structural and signaling properties to the basement membrane. Of the 12 known ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.20563

    authors: Miner JH

    更新日期:2008-05-01 00:00:00

  • Raman microscopy of freeze-dried mouse eyeball-slice in conjunction with the "in vivo cryotechnique".

    abstract::The wavelength of Raman-scattered light depends on the molecular composition of the substance. This is the first attempt to acquire Raman spectra of a mouse eyeball removed from a living mouse, in which the eyeball was preserved using the "in vivo cryotechnique" followed by freeze-drying. Eyeballs were cryofixed using...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20449

    authors: Terada N,Ohno N,Saitoh S,Fujii Y,Ohguro H,Ohno S

    更新日期:2007-07-01 00:00:00

  • Microscopic techniques used for the identification of medicinal plants: A case study of Senna.

    abstract:BACKGROUND:The use of quality control tool for adulteration of Senna (Cassia aungustifolia) a pharmaceutically very important. They were used for multiple health disorders such as constipation, indigestion, epilepsy, asthma, piles, migraine, and heart problems. Two different species of same family or same genus used co...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23332

    authors: Shaheen S,Jaffer M,Khalid S,Khan MA,Hussain K,Butt MM,Rauf Siddiqui A,Ashfaq M,Ahmad M,Zafar M,Khan F

    更新日期:2019-10-01 00:00:00

  • Combination of multiphoton and reflective confocal imaging of cornea.

    abstract::We combine reflective confocal microscopy with multiphoton microscopy to form a minimally invasive technique to observe the cornea. The two imaging modalities allow detection of complementary information from the cornea. The autofluorescence signal shows the cytoplasm of epithelial cells, and the second harmonic gener...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20528

    authors: Chen WL,Sun Y,Lo W,Tan HY,Dong CY

    更新日期:2008-02-01 00:00:00

  • High resolution and image processing of otoconia matrix.

    abstract::This study was designed to investigate patterns of fibrils organization in histochemically stained otoconia. Transmission electron microscope and video imaging were used. These data indicate that otoconia of the chick (Gallus domesticus) inner ear may have central cores in vivo. The data also show that the ultrastruct...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070250406

    authors: Fermin CD

    更新日期:1993-07-01 00:00:00

  • MRT letter: Micro- to nanoscale sample collection for high throughput microscopy.

    abstract::In high throughput microscopy, it is often assumed that the objects under investigation are fixed spatially. In addition, it is also presumed that the objects are sufficiently populated, otherwise there will be need to search through vast tracks of field of views before any recording can be done. The ability to collec...

    journal_title:Microscopy research and technique

    pub_type: 信件

    doi:10.1002/jemt.22238

    authors: Cheong BH,Liew OW,Ng TW

    更新日期:2013-08-01 00:00:00

  • In situ transmission electron microscopy employed for studies of effects of ion and electron irradiation on materials.

    abstract::This paper summarizes the essential features of the various facilities dedicated to in situ irradiation effects research around the world at the present time, mentions some essential techniques that are involved in this type of research, and describes very briefly some examples of studies employing these facilities an...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19980915)42:4<255::AID-JEM

    authors: Allen CW,Ryan EA

    更新日期:1998-08-15 00:00:00

  • Monolayer and three-dimensional cell culture and living tissue culture of gallbladder epithelium.

    abstract::Several models for preparing and isolating human and animal gallbladder epithelial cells, including low-grade gallbladder carcinoma cells, as well as proposed systems for culturing these isolated epithelial cells are reviewed here. Several reports concerning tissue culture of the gallbladder are also reviewed. The cel...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19971001)39:1<71::AID-JEMT

    authors: Nakanuma Y,Katayanagi K,Kawamura Y,Yoshida K

    更新日期:1997-10-01 00:00:00

  • Histological description of immature Chironomus columbiensis (Diptera: Chironomidae): A potential contribution to environmental monitoring.

    abstract::Chironomids have been widely used in environmental monitoring and toxicity assays. Assessment criteria for chironomids range from the molecular-biochemical level to the population level. However, the use of markers to evaluate cellular and anatomical changes in organs and systems of individuals under contaminant expos...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23278

    authors: Ospina-Pérez EM,Campeón-Morales OI,Richardi VS,Rivera-Páez FA

    更新日期:2019-08-01 00:00:00

  • Morphological investigation of various orthodontic lingual bracket slots using scanning electron microscopy and atomic force microscopy.

    abstract::Various labial and lingual orthodontic appliances with aesthetic materials have been developed due to an increased demand in aesthetic orthodontic treatment. However, there are few reports regarding the morphology of lingual orthodontic appliances. Therefore, this study evaluates the roughness of slot surfaces of vari...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22775

    authors: Choi S,Kim JH,Kim NJ,Lee SH,Kim H,Kim KA,Park KH,Park YG

    更新日期:2016-12-01 00:00:00

  • Organ culture of the bovine subcommissural organ: evidence for synthesis and release of the secretory material.

    abstract::The subcommissural organ (SCO) is a brain circumventricular organ formed by ependymal and hypendymal secretory cells. It secretes glycoproteins into the cerebrospinal fluid of the third ventricle where they condense into a thread-like structure known as Reissner's fiber (RF). The present study was designed to investig...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20010301)52:5<496::AID-JEMT1035>

    authors: Schöbitz K,Gonzalez C,Peruzzo B,Yulis CR,Rodríguez EM

    更新日期:2001-03-01 00:00:00

  • Synaptic prion protein immuno-reactivity in the rodent cerebellum.

    abstract::The cellular prion protein PrP(c) is a neurolemmal glycoprotein essential for the development of the transmissible spongiform encephalopathies. In these neurodegenerative diseases, host PrP(c) is converted to infectious protease-resistant isoforms PrP(res) or prions. Prions provoque predictable and distinctive pattern...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/1097-0029(20000701)50:1<66::AID-JEMT10>3.0

    authors: Haeberlé AM,Ribaut-Barassin C,Bombarde G,Mariani J,Hunsmann G,Grassi J,Bailly Y

    更新日期:2000-07-01 00:00:00

  • Effects of vitamin A deficiency on the inter-Sertoli cell tight junctions and on the germ cell population.

    abstract::When 20-day-old rats are placed on a vitamin A deficient diet (VAD) for a period of 10 weeks, the seminiferous tubules are found to contain only Sertoli cells, a few residual A0, A1 spermatogonia, and preleptotene spermatocytes (PL). The type A1 spermatogonia and PL spermatocytes are arrested in their G2 phase. In VAD...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070200106

    authors: Ismail N,Morales CR

    更新日期:1992-01-01 00:00:00

  • Pododermal angioarchitecture of the bovine claw in relation to form and function of the papillary body: a scanning electron microscopic study.

    abstract::The region-specific angioarchitecture of the bovine pododerma was examined using systematic scanning electron microscopy of micro-corrosion casts of juvenile and adult bovine claws. Particular emphasis was laid on the demonstration of specialised vascular structures such as arteriovenous anastomoses. Comparing the res...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1150

    authors: Hirschberg RM,Mülling CK,Budras KD

    更新日期:2001-09-15 00:00:00

  • Apatite-like forming ability, porosity, and bond strength of calcium aluminate cement with chitosan, zirconium oxide, and hydroxyapatite additives.

    abstract::This study evaluated the effect of chitosan, zirconium oxide, and hydroxyapatite on the apatite-like forming ability, porosity, and bond-strength of calcium-aluminate cements (C). Three hundred bovine root-slices were assigned to one of five groups, according to the material: MTA, C, C + chitosan (Cchi), C + zirconium...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23678

    authors: Saltareli FM,Leoni GB,de Lima Ferraz Aguiar N,de Faria NS,Oliveira IR,Bachmann L,Raucci-Neto W

    更新日期:2020-12-22 00:00:00

  • Response of donor and recipient cells after transplantation of cells to the ligament and tendon.

    abstract::The mechanical properties of healing ligaments and tendons are not comparable to those of normal tissue. To improve the quality of the ligament healing, therapeutic strategies include gene transfer or placement of mesenchymal stem cells at the healing site. Studies show that marker genes, growth factors, and antisense...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.10114

    authors: Hildebrand KA,Jia F,Woo SL

    更新日期:2002-07-01 00:00:00

  • Role of PO4 tetrahedron in LiFePO4 and FePO4 system.

    abstract::Using high resolution transmission electron microscopy with image simulation and Fourier analysis, the Li1- x FePO4 (x < 0.01), Li1- x FePO4 (x ∼ 0.5), and FePO4 particles, prepared by charging or discharging the 053048 electrochemical cells (thickness: 5 mm, width: 30 mm, height: 48 mm) and dismantled inside an Ar-fi...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22495

    authors: Zeng Y

    更新日期:2015-06-01 00:00:00

  • Perivitelline space: does it play a role in blocking polyspermy in mammals?

    abstract::The perivitelline space of mammalian oocytes changes in size and composition during preimplantation development. Often overlooked in the past, this space contains a hyaluronan-rich extracellular matrix prior to fertilization and a cortical granule envelope following release of the cortical granules at fertilization. T...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10348

    authors: Talbot P,Dandekar P

    更新日期:2003-07-01 00:00:00

  • Efficiency of spermatogenesis.

    abstract::Spermatogenesis is a process of division and differentiation by which spermatozoa are produced in seminiferous tubules. A measure of efficiency of spermatogenesis is the estimated number of spermatozoa produced per day per gram of testicular parenchyma. This measure is not influenced by species differences in testicul...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1070320504

    authors: Johnson L

    更新日期:1995-12-01 00:00:00

  • Peripheral and central structures involved in insect gustation.

    abstract::Studies in insect gustation have a long history in general physiology, particularly with work on fly labellar and tarsal sensilla and in the general field of insect-plant interactions, where work on immature Lepidoptera and chrysomelid beetles has been prominent. Much more emphasis has been placed on the physiological...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-0029(19991215)47:6<401::AID-JEM

    authors: Mitchell BK,Itagaki H,Rivet MP

    更新日期:1999-12-15 00:00:00

  • Introduction to olfactory neuroepithelium.

    abstract::Among the five senses, the sense of smell (olfaction) is the most sensitive and emotional window on the outside world (Stern and Marx, 1999). The olfactory system recognizes and discriminates myriad odorants of diverse molecular structures. What makes the olfactory system so specific and sensitive? OE harboring the ol...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.10130

    authors: Nibu K

    更新日期:2002-08-01 00:00:00

  • Retinal characterization in the eyes of two bats endemic in the Egyptian fauna, the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrellus kuhlii), using the light microscope and transmission electron microscope.

    abstract::Bats are the only mammals that can fly in the dark without eye usage. This study was conducted to describe the structural and functional adaptations of the retina of two bats very common in the Egyptian fauna having a different lifestyle: the Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrell...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.23530

    authors: Aboelnour A,Noreldin AE,Massoud D Dr,Abumandour MMA

    更新日期:2020-11-01 00:00:00

  • Fossil record of echinoderm regeneration with special regard to crinoids.

    abstract::Regeneration of skeletal parts of fossil echinoderms is reviewed. Among regenerations of various fossil echinoderms, those of crinoids are most common and many examples have been reported. Mesozoic and Cenozoic crinoids, the subclass Articulata, are characterized by the dominance of muscular articulations in the arms ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章,评审

    doi:10.1002/jemt.1186

    authors: Oji T

    更新日期:2001-12-15 00:00:00

  • Effect of Aloe vera application on the content and molecular arrangement of glycosaminoglycans during calcaneal tendon healing.

    abstract::Although several treatments for tendon lesions have been proposed, successful tendon repair remains a great challenge for orthopedics, especially considering the high incidence of re-rupture of injured tendons. Our aim was to evaluate the pharmacological potential of Aloe vera on the content and arrangement of glycosa...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.22422

    authors: Aro AA,Esquisatto MA,Nishan U,Perez MO,Rodrigues RA,Foglio MA,Carvalho JE,Gomes L,Vidal Bde C,Pimentel ER

    更新日期:2014-12-01 00:00:00

  • In situ hybridization studies confirming recent findings of the existence of a local nonneuronal catecholamine production in human patellar tendinosis.

    abstract::We have in recent studies presented unexpected immunohistochemical evidence favoring the existence of a local production of catecholamines, and an occurrence of adrenergic receptors on the tendon cells (tenocytes), in the human patellar tendon. This was particularly noticed for tendons from patients suffering from ten...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.20495

    authors: Danielson P,Alfredson H,Forsgren S

    更新日期:2007-10-01 00:00:00

  • Ultrastructural and functional connectivity of intracellularly stained neurones in the vertebrate retina: correlative analyses.

    abstract::A variety of intracellular recording and staining techniques has been used to establish structure-function and, in some cases, structure-function-neurochemical correlations in fish, turtle, and cat retinae. Cone photoreceptor-horizontal cell connectivity has been studied extensively in the cyprinid fish retina by intr...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070240106

    authors: Djamgoz MB,Kolb H

    更新日期:1993-01-01 00:00:00

  • Section directed cryosectioning of specimens for scanning electron microscopy: a new method to study cardiac development.

    abstract::A new method to study the developing heart was developed. Using this "section directed" cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with ...

    journal_title:Microscopy research and technique

    pub_type: 杂志文章

    doi:10.1002/jemt.1070300606

    authors: Seo JW,Kim EK,Brown NA,Wessels A

    更新日期:1995-04-15 00:00:00